##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552083_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	460078
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.138650402757794	28.0	16.0	31.0	16.0	33.0
2	25.8515534322441	28.0	19.0	31.0	16.0	33.0
3	26.288592369120018	30.0	23.0	33.0	16.0	33.0
4	29.213689852590214	32.0	19.0	35.0	19.0	37.0
5	31.259288642360644	33.0	28.0	35.0	19.0	37.0
6	29.997243945591833	33.0	28.0	35.0	17.0	37.0
7	31.949910667321628	33.0	31.0	35.0	28.0	36.0
8	32.72202322214929	35.0	32.0	35.0	28.0	37.0
9	34.106021152934936	35.0	32.0	37.0	28.0	39.0
10	33.662444194245325	35.0	32.0	39.0	27.0	39.0
11	34.19288903185981	35.0	32.0	39.0	27.0	39.0
12	34.396484943857345	37.0	32.0	39.0	27.0	39.0
13	34.62637639704572	37.0	33.0	39.0	27.0	39.0
14	35.08786118875495	37.0	33.0	39.0	27.0	41.0
15	35.446393872343386	37.0	33.0	40.0	27.0	41.0
16	35.255524063311	37.0	33.0	40.0	27.0	41.0
17	34.2331626376397	36.0	32.0	39.0	25.0	41.0
18	34.338166571755224	36.0	32.0	39.0	27.0	40.0
19	33.85537452345037	37.0	32.0	39.0	25.0	40.0
20	33.793421984967765	35.0	32.0	38.0	25.0	40.0
21	34.752731058646575	36.0	33.0	39.0	27.0	40.0
22	35.521009481001045	37.0	34.0	39.0	30.0	40.0
23	36.00091506222858	37.0	34.0	40.0	30.0	40.0
24	35.293995800712054	36.0	34.0	39.0	30.0	40.0
25	34.19806641482531	36.0	33.0	39.0	25.0	40.0
26	34.639263342302826	36.0	33.0	39.0	27.0	40.0
27	35.19239129017254	37.0	34.0	39.0	30.0	40.0
28	35.18536856793848	37.0	34.0	39.0	28.0	40.0
29	35.2962910636892	37.0	34.0	40.0	27.0	40.0
30	34.25722594864349	36.0	33.0	39.0	24.0	40.0
31	34.40758306200253	36.0	33.0	39.0	25.0	40.0
32	34.10947926221206	36.0	33.0	39.0	24.0	40.0
33	33.81615943383513	37.0	33.0	39.0	21.0	40.0
34	34.023059133451284	37.0	33.0	40.0	21.0	40.0
35	33.820862984102696	37.0	33.0	40.0	18.0	41.0
36	33.746614704463155	37.0	33.0	40.0	18.0	41.0
37	33.74638865583662	37.0	33.0	40.0	16.0	40.0
38	33.39309856154826	37.0	33.0	40.0	16.0	41.0
39	33.35088180699795	37.0	33.0	40.0	15.0	40.0
40	32.77795286886137	36.0	31.0	39.0	15.0	40.0
41	32.54532057607623	36.0	31.0	39.0	15.0	40.0
42	33.04605523411248	37.0	33.0	40.0	15.0	40.0
43	33.13652032916158	37.0	33.0	40.0	15.0	40.0
44	33.449873717065365	37.0	33.0	40.0	15.0	40.0
45	33.42502575650216	37.0	33.0	40.0	17.0	40.0
46	33.17700042166763	36.0	33.0	40.0	17.0	40.0
47	33.015958163615736	36.0	32.0	40.0	17.0	40.0
48	32.97679741261264	36.0	32.0	39.0	15.0	40.0
49	33.095244719373675	36.0	33.0	40.0	15.0	40.0
50	33.087798155964855	36.0	33.0	40.0	15.0	40.0
51	31.842313694634388	35.0	30.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	7.0
10	11.0
11	11.0
12	17.0
13	27.0
14	39.0
15	63.0
16	128.0
17	279.0
18	525.0
19	959.0
20	1604.0
21	2397.0
22	3543.0
23	5078.0
24	7567.0
25	11086.0
26	13369.0
27	14159.0
28	14019.0
29	15373.0
30	19039.0
31	24267.0
32	31614.0
33	39969.0
34	53032.0
35	70625.0
36	70136.0
37	46695.0
38	13741.0
39	694.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.098174657340714	13.357300283864909	16.57588495863745	25.968640100156932
2	34.33874256104399	32.521007307456564	18.89157925395259	14.248670877546852
3	22.4990110372589	30.994527014984413	32.99853503101648	13.507926916740203
4	20.381761353509624	17.873273662292046	47.42587126530719	14.319093718891143
5	29.08115580401584	20.683666682605992	32.57360708401619	17.661570429361976
6	18.990692882511226	33.29348501775786	34.45415777324715	13.261664326483771
7	60.69057855407126	3.2924851872943286	32.459278644056006	3.5576576145783974
8	57.769117410526036	12.27465777542069	23.80878894448333	6.147435869569942
9	53.285747199387934	3.8139185094701338	22.776355313664205	20.123978977477734
10	33.33282617295328	16.905394302705194	31.525523932898338	18.23625559144319
11	25.272671155760545	17.958911315037884	36.51359117367055	20.25482635553102
12	20.73257143353953	14.951377809849633	42.01265872308609	22.303392033524748
13	19.651450406235465	17.15296101965319	47.84210503436374	15.353483539747609
14	17.050152365468463	24.079612587430827	38.509774429553254	20.36046061754746
15	14.395602484796054	19.221740661366116	47.990775477201694	18.391881376636135
16	16.849968918313852	21.340294471806953	35.805885089050115	26.003851520829073
17	17.292937284547403	21.546346489073592	39.2737753163594	21.886940910019607
18	17.6441820734745	21.26617660483657	38.107451345206684	22.982189976482246
19	17.31271653936941	23.761623029138537	34.32939631975447	24.596264111737575
20	19.659927229730613	22.649855024582788	40.85720247436304	16.83301527132356
21	19.660144584179204	25.950817035372264	36.83897078321502	17.55006759723351
22	17.71069253474411	17.91565777976778	38.42044175118132	25.953207934306793
23	19.231738966001416	24.484543925160516	37.96421476358356	18.319502345254502
24	22.385769369541684	19.91597077017375	33.98554158207956	23.712718278205
25	17.244467242511053	28.781424019405407	32.47231991097162	21.501788827111927
26	17.895661170497178	18.791378853150988	37.09001517134051	26.222944805011323
27	23.108690265563666	20.49348154008668	32.28300418624668	24.114824008102975
28	17.476384439160316	20.409148014032404	37.56276109703137	24.551706449775907
29	22.695716813236018	18.29168097583453	32.530788257643266	26.48181395328618
30	24.584092262616338	22.86047148526989	33.70841466012285	18.847021591990924
31	26.595055621003393	20.035950425797363	29.539773690548127	23.829220262651116
32	24.22219710570816	29.097240033211758	28.929877107794766	17.75068575328531
33	20.627371880420277	26.093618908098193	28.028725563926116	25.250283647555417
34	21.686322753967804	23.654032577084756	28.09654015188729	26.56310451706015
35	22.1445059316029	29.462830215746028	26.91760962271615	21.475054229934923
36	23.352996665782758	29.311334165076357	26.02210929451093	21.313559874629952
37	21.869335199683533	28.1530523085216	30.545907433087432	19.431705058707436
38	23.083911858424006	29.20722138420007	25.62761097031373	22.081255787062194
39	23.30539604154078	26.014936597707344	27.126921956711687	23.552745404040184
40	20.5363003664596	29.281773960067643	30.58655271497442	19.59537295849834
41	19.094370954490326	30.47309369280861	27.49468568373189	22.937849668969175
42	20.273084129212872	25.97059629019427	33.902946891614036	19.853372688978826
43	23.572307304413602	24.37543199196658	27.8287594712201	24.223501232399723
44	23.68120188315894	21.376810019170662	29.47804502714757	25.463943070522827
45	21.25117914788362	20.447185042536265	30.51526045583575	27.786375353744365
46	26.332926155999637	25.026843274401294	28.216085098613714	20.424145470985355
47	18.529032033698634	21.83999234912341	38.64431683323263	20.98665878394533
48	22.183629732349733	22.713757232469277	29.452179847764942	25.65043318741605
49	19.00264737718387	21.006872747664527	37.01807084885607	22.97240902629554
50	20.01899677880707	20.304383169810336	34.3152682805959	25.361351770786694
51	20.460443663900467	19.56711688018119	30.850203661118332	29.122235794800012
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	79.0
1	303.5
2	528.0
3	9775.5
4	19023.0
5	12861.0
6	6699.0
7	6445.5
8	6192.0
9	6023.5
10	5855.0
11	5598.0
12	5341.0
13	5033.5
14	4726.0
15	4398.0
16	4070.0
17	3805.5
18	3541.0
19	3328.0
20	3115.0
21	2894.0
22	2673.0
23	2570.5
24	2468.0
25	2530.5
26	2832.0
27	3071.0
28	3431.0
29	3791.0
30	4072.0
31	4353.0
32	4850.0
33	5347.0
34	5962.5
35	6578.0
36	7284.5
37	7991.0
38	8853.5
39	9716.0
40	10536.5
41	11357.0
42	12591.5
43	13826.0
44	16323.5
45	18821.0
46	52715.0
47	86609.0
48	62031.0
49	37453.0
50	35702.0
51	33951.0
52	29904.0
53	25857.0
54	24202.0
55	22547.0
56	20780.5
57	19014.0
58	18131.0
59	17248.0
60	16491.0
61	15734.0
62	14796.5
63	13859.0
64	12621.0
65	11383.0
66	9815.5
67	8248.0
68	7035.0
69	5822.0
70	4970.0
71	4118.0
72	3406.0
73	2694.0
74	2186.5
75	1335.5
76	992.0
77	778.0
78	564.0
79	435.5
80	307.0
81	236.0
82	165.0
83	103.5
84	42.0
85	33.5
86	25.0
87	16.5
88	8.0
89	4.5
90	1.0
91	0.5
92	0.0
93	1.0
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	460078.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.69829709140911
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.62216741925143	34.5580427342123
2	9.708104488437774	8.872876862141434
3	5.015350640668525	6.875789507845778
4	3.120857375839607	5.704714701641351
5	2.080021340771205	4.752671659351682
6	1.3791174630411867	3.7813991730003944
7	0.9342634166263406	2.988597302223781
8	0.6335458849311986	2.3161574456500484
9	0.42039225462313723	1.7290089132025888
>10	1.0229834800781155	6.930984393742021
>50	0.03257036764626486	0.9923476083155534
>100	0.021875620060897674	2.191739759040063
>500	0.003402874231695194	1.115863451212065
>1k	0.002916749341453023	3.3289296867236304
>5k	4.8612489024217056E-4	1.2169420636551251
>10k+	0.0019444995609686822	12.643934738042187
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTC	19501	4.238629102021831	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCG	16073	3.4935380522433155	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGC	10952	2.3804659209960053	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10390	2.258312720886458	No Hit
GAACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT	5478	1.1906676693951896	No Hit
GCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGC	3601	0.7826933693851913	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCT	2476	0.5381696147175044	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGGAACAAT	2446	0.5316489812596995	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTC	2223	0.4831789392233491	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGGAACAATCGTA	2142	0.46557322888727565	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTT	2097	0.45579227870056815	No Hit
GAATGCTGCCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTT	919	0.19974873825742592	No Hit
GAATCTTTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTC	839	0.18236038236994595	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCC	753	0.163667899790905	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGGAACAATCGT	749	0.16279848199653102	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCGGAACAATCGTATG	642	0.1395415559970266	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCGGAACAATCGTAT	604	0.1312820869504736	No Hit
GAACTGTCCCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCT	517	0.11237224992283916	No Hit
CTGTCTCTTATACACATCTGACGCCGGAACAATCGTATGCCGTCTTCTGCT	467	0.10150452749316421	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10824251539956269	0.0
2	0.0	0.0	0.0	1.0904672685935863	0.0
3	0.0	0.0	0.0	1.36585535496155	0.0
4	0.0	0.0	0.0	2.9751476923478193	0.0
5	0.0	0.0	0.0	8.15405213898513	0.0
6	0.0	0.0	0.0	9.33667769378236	0.0
7	0.0	0.0	0.0	10.505392563869604	0.0
8	0.0	0.0	0.0	11.70084203113385	0.0
9	0.0	0.0	0.0	12.184238324805793	0.0
10	0.0	0.0	0.0	16.529588461087034	0.0
11	0.0	0.0	0.0	17.380096418433396	0.0
12	0.0	0.0	0.0	20.606288498906707	0.0
13	0.0	0.0	0.0	21.08838066588709	0.0
14	0.0	0.0	0.0	21.378983563656597	0.0
15	0.0	0.0	0.0	22.0543038354366	0.0
16	0.0	0.0	0.0	22.50596637961389	0.0
17	0.0	0.0	0.0	22.958933050482745	0.0
18	0.0	0.0	0.0	23.420593899295337	0.0
19	0.0	0.0	0.0	24.275666300062163	0.0
20	0.0	0.0	0.0	24.636257330278777	0.0
21	0.0	0.0	0.0	24.947291546216075	0.0
22	0.0	0.0	0.0	25.373958328805116	0.0
23	0.0	0.0	0.0	25.67499424010711	0.0
24	0.0	0.0	0.0	25.934080742830563	0.0
25	0.0	0.0	0.0	26.165780585031232	0.0
26	0.0	0.0	0.0	26.444646342576693	0.0
27	0.0	0.0	0.0	26.74263929159838	0.0
28	0.0	0.0	0.0	26.956950777911572	0.0
29	0.0	0.0	0.0	27.173870517607885	0.0
30	0.0	0.0	0.0	27.421437234555878	0.0
31	0.0	0.0	0.0	27.65313707675655	0.0
32	0.0	0.0	0.0	27.88657575454597	0.0
33	0.0	0.0	0.0	28.12327474906429	0.0
34	0.0	0.0	0.0	28.364538187003074	0.0
35	0.0	0.0	0.0	28.623842044175117	0.0
36	0.0	0.0	0.0	28.854889823030007	0.0
37	0.0	0.0	0.0	29.09658796986598	0.0
38	0.0	0.0	0.0	29.366976903916292	0.0
39	0.0	0.0	0.0	29.6375831924152	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTACGG	30	2.162653E-6	45.000004	2
CTATGCG	20	7.02887E-4	45.0	1
TCGCACG	25	3.887146E-5	45.0	35
CGAACAC	20	7.02887E-4	45.0	34
CGCTATC	20	7.02887E-4	45.0	26
TAACAGG	35	1.2098099E-7	45.0	2
CGCTACG	20	7.02887E-4	45.0	3
CGGTCTA	25	3.887146E-5	45.0	31
CTCTACG	25	3.887146E-5	45.0	1
CGTTTTT	6885	0.0	44.183006	1
TACGGCT	1215	0.0	42.03704	7
GAATCTG	2305	0.0	41.095444	1
ACGGCTG	1245	0.0	41.024097	8
AAGGGCG	145	0.0	40.344826	5
GATACCT	1970	0.0	40.317257	5
TGATACC	2005	0.0	40.062344	4
TTAACGG	45	1.9239451E-8	40.000004	2
AATCTGT	2380	0.0	39.80042	2
CGTTAGG	40	3.4521872E-7	39.375	2
CGACCAC	1030	0.0	39.10194	12
>>END_MODULE