SRR3552079_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552079_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	412164
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC	23982	5.818557661513378	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCG	18045	4.378111625469473	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGC	13223	3.2081889733212994	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8236	1.9982337127939362	No Hit
GAACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT	6785	1.6461893809260393	No Hit
GCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	5433	1.318164614085655	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCT	3115	0.7557671218252928	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTC	3045	0.7387835909977581	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCTTGTAT	2963	0.7188885977426461	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTA	2690	0.6526528275152609	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTT	2523	0.6121349753981425	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCC	1220	0.2959986801370328	No Hit
GAATGCTGCCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTT	915	0.22199901010277462	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATG	762	0.18487786415116314	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGT	758	0.18390737667530402	No Hit
CTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGCT	752	0.18245164546151532	Illumina Paired End PCR Primer 2 (95% over 21bp)
GAATCTTTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC	732	0.17759920808221968	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTAT	612	0.14848458380644597	No Hit
CCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	592	0.14363214642715036	TruSeq Adapter, Index 14 (95% over 21bp)
GAACTGTCCCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT	502	0.12179617822032007	No Hit
GACTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC	492	0.11936995953067227	No Hit
CGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTG	476	0.11548800962723575	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGC	447	0.10845197542725712	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCCCTTGTA	435	0.10554051299967973	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTAG	35	1.2093915E-7	45.0	1
ACCGGTG	40	6.7975634E-9	45.0	17
CGAACCC	25	3.8864375E-5	45.0	34
TCACATG	25	3.8864375E-5	45.0	22
GGATATC	20	7.028015E-4	45.0	8
TATTGCG	20	7.028015E-4	45.0	1
CTACGAC	20	7.028015E-4	45.0	5
AGCGACG	20	7.028015E-4	45.0	1
CTATTCG	20	7.028015E-4	45.0	26
CGGTAGT	25	3.8864375E-5	45.0	12
CACCGGT	55	1.8189894E-12	45.0	16
ACGTAGG	30	2.1621017E-6	44.999996	2
ATCGGGT	30	2.1621017E-6	44.999996	4
CGTTTTT	5085	0.0	43.80531	1
TACGGCT	1470	0.0	42.704082	7
ACGGCTG	1505	0.0	41.561462	8
ACGGGCC	65	0.0	41.538464	5
AGCGCAA	65	0.0	41.538464	21
CGCAAGT	65	0.0	41.538464	23
TGATCCG	60	3.6379788E-12	41.249996	4

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content