##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552079_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	412164
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.119224386409293	28.0	16.0	31.0	16.0	33.0
2	25.646441707669762	28.0	16.0	31.0	16.0	33.0
3	26.032176512262108	30.0	16.0	31.0	16.0	33.0
4	29.30951999689444	32.0	25.0	35.0	19.0	37.0
5	31.255592434079638	33.0	28.0	35.0	19.0	37.0
6	30.175643675818364	33.0	28.0	35.0	17.0	37.0
7	31.88985209770868	33.0	31.0	35.0	28.0	35.0
8	32.46505031977562	35.0	32.0	35.0	27.0	37.0
9	33.947353480653334	35.0	32.0	37.0	28.0	39.0
10	33.74361904484623	35.0	32.0	39.0	27.0	39.0
11	34.39372676895605	37.0	32.0	39.0	27.0	39.0
12	34.50625964421929	37.0	33.0	39.0	27.0	39.0
13	34.73741762987549	37.0	33.0	39.0	29.0	39.0
14	35.16198891703303	37.0	34.0	39.0	27.0	41.0
15	35.42851631874691	37.0	33.0	40.0	27.0	41.0
16	35.05463359245349	37.0	32.0	40.0	27.0	41.0
17	34.10591415067788	36.0	32.0	39.0	25.0	41.0
18	34.17368814355451	36.0	32.0	39.0	26.0	40.0
19	33.65181092962995	36.0	32.0	38.0	25.0	40.0
20	33.59748546695005	35.0	32.0	38.0	25.0	40.0
21	34.79154656884153	36.0	33.0	39.0	27.0	40.0
22	35.60164157956542	37.0	34.0	39.0	30.0	40.0
23	36.01486058947409	37.0	34.0	40.0	30.0	40.0
24	35.394432798594735	37.0	34.0	39.0	30.0	40.0
25	34.154094001416915	36.0	32.0	39.0	25.0	40.0
26	34.68908492735901	36.0	33.0	39.0	27.0	40.0
27	35.29506701216021	37.0	34.0	39.0	30.0	40.0
28	35.267721101309185	37.0	34.0	39.0	27.0	40.0
29	35.409332207567864	38.0	34.0	40.0	27.0	40.0
30	34.58797226346794	37.0	33.0	40.0	25.0	40.0
31	34.62561262021914	36.0	33.0	39.0	26.0	40.0
32	34.526091555788476	37.0	33.0	39.0	26.0	40.0
33	34.412297046806614	37.0	34.0	39.0	24.0	40.0
34	34.44724915324968	38.0	34.0	40.0	23.0	40.0
35	34.26743238128512	38.0	34.0	40.0	21.0	41.0
36	34.24743306062635	38.0	34.0	40.0	20.0	41.0
37	34.340478547374346	38.0	34.0	40.0	20.0	40.0
38	33.94174406304287	37.0	33.0	40.0	19.0	40.0
39	33.742306460535126	37.0	33.0	40.0	18.0	40.0
40	33.225910074630484	37.0	33.0	40.0	17.0	40.0
41	32.764489378014574	36.0	31.0	39.0	15.0	40.0
42	33.466998573383414	37.0	33.0	40.0	17.0	40.0
43	33.566980619365104	37.0	33.0	40.0	18.0	40.0
44	33.962621674867286	37.0	34.0	40.0	18.0	40.0
45	33.97672528411021	37.0	34.0	40.0	20.0	40.0
46	33.68473956968585	37.0	33.0	40.0	18.0	40.0
47	33.47634679399462	37.0	33.0	40.0	18.0	40.0
48	33.466268281557824	37.0	33.0	40.0	18.0	40.0
49	33.610261934569735	37.0	33.0	40.0	18.0	40.0
50	33.54609815510331	37.0	33.0	40.0	18.0	40.0
51	32.05056724992964	35.0	31.0	38.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	8.0
10	10.0
11	11.0
12	19.0
13	20.0
14	32.0
15	46.0
16	90.0
17	208.0
18	442.0
19	758.0
20	1334.0
21	2064.0
22	2915.0
23	4345.0
24	6203.0
25	8540.0
26	10884.0
27	11571.0
28	11578.0
29	12937.0
30	15926.0
31	20870.0
32	27579.0
33	36141.0
34	49180.0
35	67345.0
36	66447.0
37	42391.0
38	11771.0
39	496.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.684416882600125	11.492027445385817	16.014499082889337	24.80905658912472
2	31.535020040566376	36.9571335681913	18.15345347968284	13.354392911559476
3	21.030706223736182	35.17725953746567	31.251637697615514	12.540396541182636
4	19.2188061063072	16.360963111771042	50.43768985161247	13.982540930309295
5	30.91876049339583	18.660533185819236	31.65293426888326	18.76777205190167
6	17.848477790394117	34.613891557729445	34.13762482895159	13.400005822924854
7	57.50769111324618	3.078628895294106	35.25950835104473	4.15417164041498
8	52.474015197833864	14.793625838258556	24.777515746159295	7.954843217748275
9	48.03379237390942	3.7861142651954074	23.32445337292922	24.855639987965954
10	32.47129783290147	15.72408070573849	32.168990984171344	19.63563047718869
11	24.2794130491746	16.91899341039004	36.68879378111625	22.112799759319106
12	19.601906037402586	13.843761221261438	42.836346696945874	23.717986044390095
13	18.495550314923186	16.83431837812133	49.58535922593919	15.084772081016295
14	16.37552042390893	25.461952038508944	36.27148416649683	21.8910433710853
15	12.771615182306073	19.101862365466175	49.86777108141419	18.25875137081356
16	14.120107530012325	22.23580904688425	34.04542851874496	29.598654904358458
17	14.990392173988996	22.085383488126087	39.21375957143273	23.710464766452187
18	15.104667074271408	22.210091128773986	37.84270338991275	24.842538407041857
19	15.393872342077424	24.1423316932095	32.988810279403346	27.474985685309733
20	17.613377199367243	23.165050805019362	41.67297483525975	17.548597160353648
21	18.41815393872342	27.260022709406933	36.230966314379714	18.090857037489933
22	16.05428906939956	17.368571733581778	37.414475791189915	29.16266340582875
23	18.29975446666861	25.169835308275346	37.205335740142274	19.325074484913774
24	22.90059296784775	19.78265932978135	31.585728011180013	25.731019691190887
25	15.564435515959666	31.384837103677178	29.84443085761978	23.206296522743376
26	17.134926873768695	18.099348802903698	35.59165768965752	29.174066633670094
27	22.881425840199533	20.321522500752128	29.436098252152053	27.360953406896282
28	14.992333148940713	19.359526790306774	36.86881920788812	28.779320852864394
29	20.37344358071059	15.3540823555672	31.58645587678691	32.68601818693529
30	16.262943876709272	21.500422162052	33.49637522927766	28.740258731961067
31	20.22326064382139	16.614260342970276	29.40843935909007	33.75403965411826
32	20.67477994196485	20.672111101406237	37.269387913548975	21.38372104307994
33	18.591385953164274	17.865218699352685	34.552993468619285	28.990401878863754
34	26.8613949786978	17.378519230209335	31.67671121204181	24.083374579051057
35	17.758465077008182	19.610397802816355	35.30269504372046	27.328442076455
36	18.606671130909056	25.297939655088754	28.48550576954804	27.609883444454148
37	18.02486388913151	19.556050504168244	38.9260100348405	23.493075571859745
38	18.639667705088268	21.056666763715416	32.3339738550674	27.96969167612892
39	28.308391805203755	19.870245824477635	32.56980231170117	19.251560058617443
40	19.60724371851981	19.580070069195756	41.26585533913685	19.546830873147584
41	21.23717743422521	29.635048184703177	27.894721518618802	21.233052862452812
42	19.788482254636506	19.991556758960023	40.48898011471162	19.730980871691848
43	24.327209557360664	22.116924331091507	28.027678302811506	25.52818780873633
44	22.71620034743452	18.835463553342844	30.315117283411457	28.133218815811183
45	19.516503139526982	16.941072000465834	31.771333740938072	31.771091119069105
46	27.951737657825525	22.540056870566087	29.30581030851797	20.20239516309042
47	17.17787094457546	18.045486747993518	43.19251560058618	21.58412670684485
48	22.468968662959405	20.222047534476566	29.512281519007	27.79670228355703
49	18.207072912724062	16.98086198697606	41.21975718403354	23.592307916266343
50	20.22738521559379	17.182238138216828	35.923807028270296	26.666569617919077
51	20.276394833124677	16.605040711949613	30.780223406216944	32.33834104870877
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	57.0
1	221.5
2	386.0
3	7251.0
4	14116.0
5	9813.5
6	5511.0
7	5248.5
8	4986.0
9	4816.0
10	4646.0
11	4482.0
12	4318.0
13	4019.0
14	3720.0
15	3457.0
16	3194.0
17	2932.5
18	2671.0
19	2519.5
20	2368.0
21	2244.0
22	2120.0
23	2046.5
24	1973.0
25	1980.0
26	2131.0
27	2275.0
28	2499.0
29	2723.0
30	3104.0
31	3485.0
32	3917.0
33	4349.0
34	4797.0
35	5245.0
36	5668.5
37	6092.0
38	6744.5
39	7397.0
40	8408.5
41	9420.0
42	10761.0
43	12102.0
44	14814.5
45	17527.0
46	57362.5
47	97198.0
48	67194.0
49	37190.0
50	35150.0
51	33110.0
52	27336.5
53	21563.0
54	19464.5
55	17366.0
56	16159.0
57	14952.0
58	14094.5
59	13237.0
60	12671.0
61	12105.0
62	11388.5
63	10672.0
64	9943.0
65	9214.0
66	8275.5
67	7337.0
68	6273.5
69	5210.0
70	4474.0
71	3738.0
72	3159.0
73	2580.0
74	2126.5
75	1428.0
76	1183.0
77	922.0
78	661.0
79	472.0
80	283.0
81	204.5
82	126.0
83	91.0
84	56.0
85	38.0
86	20.0
87	19.5
88	19.0
89	10.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	412164.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.44376242283796
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.18134681442194	33.85785678871993
2	10.307232861788219	9.161844170923676
3	5.121771597389418	6.828924001752437
4	3.0901090739036565	5.493442941649198
5	1.9271370011307312	4.282460951725732
6	1.1440677423555652	3.050800496213
7	0.7235384805916449	2.250974063463736
8	0.43641049723465686	1.551657956634374
9	0.28116023017308683	1.1246236625306798
>10	0.7216157828947426	5.059627247647244
>50	0.029468861526836164	0.8735499112859838
>100	0.025020731485049557	2.522545260086936
>500	0.004448130041786587	1.390019307208955
>1k	0.0033360975313399405	3.8441138387453346
>5k	0.0016680487656699702	5.054480872827017
>10k+	0.0016680487656699702	13.653078528585738
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC	23982	5.818557661513378	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCG	18045	4.378111625469473	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGC	13223	3.2081889733212994	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8236	1.9982337127939362	No Hit
GAACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT	6785	1.6461893809260393	No Hit
GCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	5433	1.318164614085655	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCT	3115	0.7557671218252928	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTC	3045	0.7387835909977581	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCTTGTAT	2963	0.7188885977426461	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTA	2690	0.6526528275152609	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTT	2523	0.6121349753981425	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCC	1220	0.2959986801370328	No Hit
GAATGCTGCCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTT	915	0.22199901010277462	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATG	762	0.18487786415116314	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGT	758	0.18390737667530402	No Hit
CTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGCT	752	0.18245164546151532	Illumina Paired End PCR Primer 2 (95% over 21bp)
GAATCTTTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC	732	0.17759920808221968	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTAT	612	0.14848458380644597	No Hit
CCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	592	0.14363214642715036	TruSeq Adapter, Index 14 (95% over 21bp)
GAACTGTCCCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT	502	0.12179617822032007	No Hit
GACTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC	492	0.11936995953067227	No Hit
CGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTG	476	0.11548800962723575	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGC	447	0.10845197542725712	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCCCTTGTA	435	0.10554051299967973	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.42621868964781E-4	0.0	0.0	0.19531060451664872	0.0
2	2.42621868964781E-4	0.0	0.0	1.8235459671392942	0.0
3	2.42621868964781E-4	0.0	0.0	2.2221736978484294	0.0
4	2.42621868964781E-4	0.0	0.0	4.49262914762085	0.0
5	2.42621868964781E-4	0.0	0.0	11.675934822061121	0.0
6	2.42621868964781E-4	0.0	0.0	13.180433031511729	0.0
7	2.42621868964781E-4	0.0	0.0	14.753835851748333	0.0
8	2.42621868964781E-4	0.0	0.0	16.538562319853263	0.0
9	2.42621868964781E-4	0.0	0.0	17.113090905561865	0.0
10	2.42621868964781E-4	0.0	0.0	22.63055482768995	0.0
11	2.42621868964781E-4	0.0	0.0	23.733513844003845	0.0
12	2.42621868964781E-4	0.0	0.0	28.12351394105259	0.0
13	2.42621868964781E-4	0.0	0.0	28.756029153443773	0.0
14	2.42621868964781E-4	0.0	0.0	29.09982434176687	0.0
15	2.42621868964781E-4	0.0	0.0	30.02445628439165	0.0
16	2.42621868964781E-4	0.0	0.0	30.56380469910036	0.0
17	2.42621868964781E-4	0.0	0.0	31.06894343028503	0.0
18	2.42621868964781E-4	0.0	0.0	31.605137760697197	0.0
19	4.85243737929562E-4	0.0	0.0	32.70130336468008	0.0
20	4.85243737929562E-4	0.0	0.0	33.14627187236149	0.0
21	4.85243737929562E-4	0.0	0.0	33.49491949806388	0.0
22	4.85243737929562E-4	0.0	0.0	34.008307372793354	0.0
23	4.85243737929562E-4	0.0	0.0	34.367630360730196	0.0
24	4.85243737929562E-4	0.0	0.0	34.65465203171553	0.0
25	4.85243737929562E-4	0.0	0.0	34.91644102832853	0.0
26	4.85243737929562E-4	0.0	0.0	35.235731407886185	0.0
27	4.85243737929562E-4	0.0	0.0	35.56035946856106	0.0
28	4.85243737929562E-4	0.0	0.0	35.789151890994845	0.0
29	4.85243737929562E-4	0.0	0.0	36.017944313428636	0.0
30	4.85243737929562E-4	0.0	0.0	36.282159528731285	0.0
31	4.85243737929562E-4	0.0	0.0	36.539823953571876	0.0
32	4.85243737929562E-4	0.0	0.0	36.76570491357809	0.0
33	4.85243737929562E-4	0.0	0.0	37.012451354315274	0.0
34	4.85243737929562E-4	0.0	0.0	37.2657485855145	0.0
35	4.85243737929562E-4	0.0	0.0	37.49890820158966	0.0
36	4.85243737929562E-4	0.0	0.0	37.72673013654759	0.0
37	4.85243737929562E-4	0.0	0.0	37.95576518085034	0.0
38	4.85243737929562E-4	0.0	0.0	38.19765918420823	0.0
39	4.85243737929562E-4	0.0	0.0	38.434943372055784	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTAG	35	1.2093915E-7	45.0	1
ACCGGTG	40	6.7975634E-9	45.0	17
CGAACCC	25	3.8864375E-5	45.0	34
TCACATG	25	3.8864375E-5	45.0	22
GGATATC	20	7.028015E-4	45.0	8
TATTGCG	20	7.028015E-4	45.0	1
CTACGAC	20	7.028015E-4	45.0	5
AGCGACG	20	7.028015E-4	45.0	1
CTATTCG	20	7.028015E-4	45.0	26
CGGTAGT	25	3.8864375E-5	45.0	12
CACCGGT	55	1.8189894E-12	45.0	16
ACGTAGG	30	2.1621017E-6	44.999996	2
ATCGGGT	30	2.1621017E-6	44.999996	4
CGTTTTT	5085	0.0	43.80531	1
TACGGCT	1470	0.0	42.704082	7
ACGGCTG	1505	0.0	41.561462	8
ACGGGCC	65	0.0	41.538464	5
AGCGCAA	65	0.0	41.538464	21
CGCAAGT	65	0.0	41.538464	23
TGATCCG	60	3.6379788E-12	41.249996	4
>>END_MODULE