##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552075_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	200777
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.77184637682603	31.0	31.0	34.0	30.0	34.0
2	31.881022228641726	33.0	31.0	34.0	30.0	34.0
3	31.162518615180026	31.0	31.0	34.0	28.0	34.0
4	35.30873556234031	37.0	35.0	37.0	33.0	37.0
5	35.52738610498214	37.0	35.0	37.0	33.0	37.0
6	35.55704089611858	37.0	35.0	37.0	33.0	37.0
7	30.128261703282746	37.0	35.0	37.0	0.0	37.0
8	31.997051455096948	37.0	33.0	37.0	17.0	37.0
9	36.020689620823106	39.0	34.0	39.0	32.0	39.0
10	36.56877032727852	38.0	35.0	39.0	32.0	39.0
11	36.93313477141306	39.0	37.0	39.0	33.0	39.0
12	37.12309178840206	39.0	37.0	39.0	34.0	39.0
13	37.09521508937777	39.0	37.0	39.0	33.0	39.0
14	38.132046997415046	40.0	37.0	41.0	33.0	41.0
15	38.33821105007048	40.0	38.0	41.0	33.0	41.0
16	38.22717243508968	40.0	37.0	41.0	33.0	41.0
17	38.07805674952808	40.0	37.0	41.0	33.0	41.0
18	37.78971694965061	39.0	37.0	40.0	33.0	41.0
19	37.379301414006584	38.0	37.0	40.0	33.0	41.0
20	37.16297185434587	39.0	35.0	40.0	33.0	41.0
21	37.06283090194594	38.0	35.0	40.0	33.0	41.0
22	37.12160755465018	38.0	35.0	40.0	33.0	41.0
23	37.07041145151088	38.0	35.0	40.0	33.0	41.0
24	36.935062283030426	38.0	35.0	40.0	33.0	41.0
25	36.588782579677954	38.0	35.0	40.0	33.0	41.0
26	36.61443292807443	38.0	35.0	40.0	33.0	41.0
27	36.63146675166976	38.0	35.0	40.0	33.0	41.0
28	36.55609457258551	38.0	35.0	40.0	33.0	41.0
29	36.47205606219836	38.0	35.0	40.0	33.0	41.0
30	36.16607978005449	38.0	35.0	40.0	31.0	41.0
31	35.88445389661166	38.0	35.0	40.0	31.0	41.0
32	35.4141460426244	38.0	35.0	40.0	28.0	41.0
33	34.750414639126994	38.0	34.0	40.0	22.0	41.0
34	34.03054134686742	38.0	34.0	40.0	18.0	41.0
35	33.552533407711046	38.0	33.0	40.0	15.0	41.0
36	33.250138213042334	38.0	33.0	40.0	10.0	41.0
37	33.035392500136965	38.0	33.0	40.0	10.0	41.0
38	32.9334734556249	37.0	33.0	40.0	10.0	41.0
39	32.83295397381174	37.0	32.0	40.0	10.0	41.0
40	32.71000164361456	37.0	32.0	40.0	10.0	41.0
41	32.544091205665985	37.0	31.0	40.0	10.0	41.0
42	32.41071935530464	37.0	31.0	40.0	10.0	41.0
43	32.325580121229024	37.0	31.0	40.0	10.0	41.0
44	32.202363816572614	37.0	31.0	40.0	10.0	41.0
45	32.14120143243499	36.0	31.0	40.0	9.0	41.0
46	32.03150759300119	36.0	31.0	40.0	9.0	41.0
47	31.95202637752332	36.0	31.0	40.0	8.0	41.0
48	31.91621550277173	36.0	30.0	40.0	8.0	41.0
49	31.858524631805437	36.0	30.0	40.0	8.0	41.0
50	31.63809101640128	35.0	29.0	40.0	8.0	41.0
51	30.18465262455361	35.0	26.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	7.0
11	7.0
12	5.0
13	7.0
14	9.0
15	9.0
16	21.0
17	59.0
18	111.0
19	189.0
20	336.0
21	588.0
22	902.0
23	1533.0
24	2567.0
25	4724.0
26	6926.0
27	7686.0
28	7111.0
29	6021.0
30	5478.0
31	5663.0
32	6421.0
33	8674.0
34	13792.0
35	17845.0
36	18257.0
37	26105.0
38	33393.0
39	26323.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.763942084999776	16.66724774252031	17.750041090363936	30.81876908211598
2	46.70256055225449	18.032443955233916	20.733450544634096	14.531544947877496
3	26.179293444966305	19.456411840001593	41.05948390502896	13.304810810003136
4	23.717856128939072	20.682647912858545	40.20231401007087	15.397181948131507
5	20.441584444433374	22.89355852512987	40.054886764918294	16.609970265518463
6	20.249829412731536	26.91792386578144	40.06534613028385	12.766900591203175
7	51.99699168729486	20.765824770765576	23.397102257728726	3.840081284210841
8	60.61003003332055	6.159072005259566	27.80896218192323	5.421935779496655
9	56.88251144304377	6.305005055359926	30.867081388804497	5.945402112791804
10	31.923477290725533	18.64805231674943	36.4210043979141	13.007465994610937
11	25.54924119794598	19.090334052207176	39.68582058701943	15.674604162827416
12	22.124048073235482	17.772454016147268	44.05185852961245	16.051639381004794
13	19.331397520632343	20.45802059000782	44.81788252638499	15.392699362974843
14	15.90122374574777	22.86915333927691	44.653521070640565	16.576101844334758
15	15.600392475233718	19.53709837282159	48.94484926062248	15.917659891322215
16	17.743068180120233	19.012635909491628	45.63421108991568	17.610084820472466
17	17.576216399288764	18.92398033639311	43.138407287687336	20.36139597663079
18	19.42254341881789	18.45779148009981	45.1695164286746	16.950148672407696
19	18.57384062915573	20.236879722278946	43.074156900441785	18.11512274812354
20	19.895705185354895	20.88037972476927	43.10752725660808	16.116387833267755
21	18.94738939221126	21.310209834791834	43.67233298634804	16.07006778664887
22	17.603111910228762	19.63173072612899	43.27537516747436	19.489782196167887
23	16.558171503708095	20.668702092371138	43.61057292418952	19.162553479731244
24	18.47173730058722	18.657515552080167	44.51157254067946	18.359174606653152
25	17.090104942299167	19.741803094976017	43.108523386642894	20.059568576081922
26	15.492810431473725	21.996045363761784	43.62352261464211	18.887621590122375
27	16.43216105430403	22.29488437420621	43.091091111033634	18.181863460456128
28	14.341782176245289	21.948729187108086	44.85224901258611	18.857239624060526
29	16.13830269403368	22.05033445065919	43.100554346364376	18.71080850894276
30	18.6191645457398	21.58563978941811	42.48644017990109	17.308755484941006
31	19.446948604670855	22.212703646333992	40.91853150510268	17.421816243892476
32	20.448059289659675	23.649621221554263	39.128485832540576	16.773833656245486
33	21.1592961345174	22.812373927292466	38.37242313611619	17.655906802073943
34	18.59077483974758	24.03861000014942	39.17630007421169	18.194315085891315
35	19.373733047111973	26.828770227665522	35.30135423878233	18.496142486440178
36	22.705289948549883	26.082668831589274	34.16178147895426	17.05025974090658
37	21.245461382528873	26.810341822021442	35.112587597184934	16.83160919826474
38	21.45166029973553	27.064354980899207	33.25878960239469	18.225195116970568
39	23.135618123589854	24.05405001568905	33.43709687862654	19.373234982094562
40	23.61027408517908	23.40108677786798	34.26687319762722	18.721765939325717
41	21.620006275619218	24.12925783331756	32.19093820507329	22.059797685989928
42	21.421278333673676	24.611882835185305	33.446560113957275	20.52027871718374
43	21.880494279723276	23.74325744482685	34.06764719066427	20.308601084785607
44	20.146231889110805	24.9087295855601	34.03079037937612	20.914248145952975
45	20.85398227884668	24.094891347116455	33.5830299287269	21.46809644530997
46	22.37407671197398	24.16711077464052	34.25093511707018	19.207877396315315
47	20.29913784945487	24.109833297638676	35.80888249152044	19.782146361386015
48	20.531236147566702	23.076846451535786	35.5224951065112	20.869422294386307
49	21.896930425297718	21.194658750753323	36.87972227894629	20.028688545002666
50	19.736324379784538	21.556752018408485	36.4747954197941	22.232128182012882
51	19.32591880544086	21.93079884648142	34.59559610911608	24.147686238961636
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	85.0
1	224.5
2	364.0
3	7322.5
4	14281.0
5	9389.5
6	4498.0
7	4140.0
8	3782.0
9	3717.0
10	3652.0
11	3487.5
12	3323.0
13	3129.5
14	2936.0
15	2753.0
16	2570.0
17	2324.0
18	2078.0
19	1924.0
20	1770.0
21	1677.5
22	1585.0
23	1544.5
24	1504.0
25	1537.0
26	1568.0
27	1566.0
28	1840.0
29	2114.0
30	2263.5
31	2413.0
32	2723.0
33	3033.0
34	3437.0
35	3841.0
36	4084.5
37	4328.0
38	4807.0
39	5286.0
40	6014.5
41	6743.0
42	8306.0
43	9869.0
44	11157.5
45	12446.0
46	13948.5
47	15451.0
48	16763.0
49	18075.0
50	17987.0
51	17899.0
52	15406.5
53	12914.0
54	11318.5
55	9723.0
56	8571.0
57	7419.0
58	6933.5
59	6448.0
60	5788.5
61	5129.0
62	4644.0
63	4159.0
64	3502.0
65	2845.0
66	2283.0
67	1721.0
68	1452.0
69	1183.0
70	1014.5
71	846.0
72	717.0
73	588.0
74	445.0
75	237.0
76	172.0
77	143.5
78	115.0
79	84.0
80	53.0
81	44.5
82	36.0
83	33.0
84	30.0
85	21.0
86	12.0
87	10.5
88	9.0
89	7.5
90	6.0
91	3.5
92	1.0
93	0.5
94	0.0
95	1.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	200777.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.96219619895558
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.62536447394977	40.0692125053582
2	9.053820874108547	9.228051914730385
3	3.3086150772531573	5.058428721413939
4	1.9624076096269922	4.000344064965368
5	1.2366133586498307	3.1510266302881043
6	0.9297087322871135	2.8427999293618886
7	0.77575693540099	2.7673994001224034
8	0.6547948345478227	2.6695826262631037
9	0.6368004726037846	2.9207475561979535
>10	2.7711317269142706	20.55782406886598
>50	0.026991542794617216	0.9241646724058459
>100	0.01499530155256512	1.4728556052509925
>500	9.99686770171008E-4	0.3693601915624246
>1k	9.99686770171008E-4	0.6638294201459851
>5k	9.99686770171008E-4	3.304372693067429
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6486	3.230449702904217	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1303	0.6489787176818063	No Hit
GCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC	725	0.36109713762034495	No Hit
CCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC	496	0.24704024863405669	No Hit
CTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGCT	374	0.18627631651035725	Illumina Single End Adapter 2 (95% over 21bp)
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	359	0.17880534124924669	No Hit
TCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC	242	0.12053173421258412	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.22761571295516916	0.0
2	0.0	0.0	0.0	1.1684605308376956	0.0
3	0.0	0.0	0.0	1.5420092938932248	0.0
4	0.0	0.0	0.0	1.9494264781324555	0.0
5	0.0	0.0	0.0	3.0232546556627504	0.0
6	0.0	0.0	0.0	3.6513146426134466	0.0
7	0.0	0.0	0.0	4.376497307958581	0.0
8	0.0	0.0	0.0	5.796480672586999	0.0
9	0.0	0.0	0.0	6.370251572640292	0.0
10	0.0	0.0	0.0	7.476453976302066	0.0
11	0.0	0.0	0.0	9.058806536605289	0.0
12	0.0	0.0	0.0	10.249181928208909	0.0
13	0.0	0.0	0.0	10.776134716625908	0.0
14	0.0	0.0	0.0	10.987314284006635	0.0
15	0.0	0.0	0.0	11.406685028663642	0.0
16	0.0	0.0	0.0	12.266843313726174	0.0
17	0.0	0.0	0.0	13.235579772583513	0.0
18	0.0	0.0	0.0	14.337797656106028	0.0
19	0.0	0.0	0.0	14.943444717273392	0.0
20	0.0	0.0	0.0	15.504763991891501	0.0
21	0.0	0.0	0.0	16.172669180234788	0.0
22	0.0	0.0	0.0	16.832605328299557	0.0
23	0.0	0.0	0.0	17.489553086259882	0.0
24	0.0	0.0	0.0	18.020988459833546	0.0
25	0.0	0.0	0.0	18.522539932362772	0.0
26	0.0	0.0	0.0	18.974284903151258	0.0
27	0.0	0.0	0.0	19.451929254844927	0.0
28	0.0	0.0	0.0	19.89122260019823	0.0
29	0.0	0.0	0.0	20.32105271022079	0.0
30	0.0	0.0	0.0	20.83704806825483	0.0
31	0.0	0.0	0.0	21.32614791534887	0.0
32	0.0	0.0	0.0	21.774904496032914	0.0
33	0.0	0.0	0.0	22.249560457622138	0.0
34	0.0	0.0	0.0	22.697320908271365	0.0
35	0.0	0.0	0.0	23.143089098850965	0.0
36	0.0	0.0	0.0	23.570926948803898	0.0
37	0.0	0.0	0.0	24.035621610044974	0.0
38	0.0	0.0	0.0	24.47292269532865	0.0
39	0.0	0.0	0.0	25.009836784093796	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGGA	20	7.0193666E-4	45.0	4
AGGACCG	25	3.8792725E-5	45.0	7
AGTGCGC	20	7.0193666E-4	45.0	11
CTTGCGG	20	7.0193666E-4	45.0	2
GGTATGC	20	7.0193666E-4	45.0	8
AGCGACT	25	3.8792725E-5	45.0	19
GGCGAGG	35	1.2052442E-7	45.0	2
GTGCGCA	25	3.8792725E-5	45.0	12
AGTGTTG	25	3.8792725E-5	45.0	1
AGGGTAC	30	2.1565265E-6	44.999996	6
TACCGGG	30	2.1565265E-6	44.999996	3
CGTTTTA	610	0.0	44.63115	1
CGTTTTT	3435	0.0	44.213974	1
GTTTTAT	660	0.0	41.931816	2
AGGCGAT	60	3.6379788E-12	41.249996	7
GGCGATA	50	1.0713848E-9	40.5	8
GGGCACC	45	1.9146682E-8	40.0	7
GTGATAG	40	3.439236E-7	39.375	1
AACGGGC	40	3.439236E-7	39.375	4
TTGGCGG	35	6.2225627E-6	38.571426	2
>>END_MODULE