FastQCFastQC Report
Sat 18 Jun 2016
SRR3552069_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552069_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194512
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTC12490.6421197663897343No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCG12180.6261824463272189No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12000.6169285185489841No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGC11610.5968783416961421No Hit
GCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCTGC7460.3835238956979518No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGACAGAGT4570.23494694414740477No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCT2800.14394998766142963No Hit
CTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCTGCT2720.13983713087110308No Hit
CCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCTGC2500.12852677469770502No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGACAGAGTCGTA2430.12492802500616929No Hit
GAACTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTCTTCT2040.1048778481533273No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGACAGAGTCGTATGCCGTC1990.1023073126593732No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGT253.8788225E-545.00000445
CGACGGT253.8788225E-545.00000428
ATAGCGG302.1561773E-645.0000042
CACGACG253.8788225E-545.00000426
CGCGAGG253.8788225E-545.0000042
CGACAAA302.1561773E-645.00000412
ACGGGCT253.8788225E-545.0000045
ATCCGGC502.1827873E-1145.0000046
TACGAAT253.8788225E-545.00000412
AGACCTA253.8788225E-545.00000432
CGAATAT253.8788225E-545.00000414
CGTAAGC253.8788225E-545.00000443
GTGCAAG406.7648216E-945.01
ATAGGGC453.8198777E-1045.04
CGACAGG351.2049713E-745.02
CCGTCGA207.018825E-445.041
ATGTACG207.018825E-445.01
GTATGGT207.018825E-445.03
GGAACAC351.2049713E-745.08
CGAATGC800.045.045