FastQCFastQC Report
Sat 18 Jun 2016
SRR3552067_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552067_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences639388
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138292.162849474810287No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCC8470.13247042484375685No Hit
CGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTG6660.10416210501291862Illumina Single End Adapter 1 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATG302.1639844E-645.0000041
ACGGGTC302.1639844E-645.0000045
TTAAGCG253.8888567E-545.01
TGTTACG253.8888567E-545.01
GCGAACG551.8189894E-1244.9999961
CGTTTTT92950.044.3948361
CGAACGG800.042.18752
GACGTAG556.002665E-1140.909091
ACGCGAG501.0804797E-940.51
CGGCGTC403.4550067E-739.3755
TCGGCGT403.4550067E-739.3754
TCACGAC356.2439394E-638.5714325
CTCACGA356.2439394E-638.5714324
CGACGGT356.2439394E-638.5714328
TAGACGG700.038.571432
GTGCTCG356.2439394E-638.571431
TTTGACG659.094947E-1238.076921
GTTTTTT110200.037.8334852
GTTTGCG301.1393552E-437.5000041
CAACGCG301.1393552E-437.5000041
Produced by FastQC (version 0.11.5)