##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552067_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	639388
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.34234924646693	33.0	31.0	34.0	30.0	34.0
2	32.52406519984736	34.0	31.0	34.0	31.0	34.0
3	32.48696096892654	34.0	31.0	34.0	31.0	34.0
4	35.99166077561669	37.0	35.0	37.0	35.0	37.0
5	36.06362959580098	37.0	35.0	37.0	35.0	37.0
6	36.08045975213798	37.0	36.0	37.0	35.0	37.0
7	36.24347031849206	37.0	37.0	37.0	35.0	37.0
8	36.080383116355016	37.0	37.0	37.0	35.0	37.0
9	37.87089060163782	39.0	38.0	39.0	35.0	39.0
10	37.71294894492859	39.0	38.0	39.0	35.0	39.0
11	37.689069860554156	39.0	37.0	39.0	35.0	39.0
12	37.696703722935055	39.0	37.0	39.0	35.0	39.0
13	37.73207661075904	39.0	38.0	39.0	35.0	39.0
14	39.069177088090484	40.0	39.0	41.0	35.0	41.0
15	39.14853265935551	41.0	39.0	41.0	35.0	41.0
16	39.19788766758212	41.0	39.0	41.0	35.0	41.0
17	39.15296189481192	41.0	39.0	41.0	35.0	41.0
18	38.89503400126371	40.0	39.0	41.0	35.0	41.0
19	38.62494604215281	40.0	37.0	41.0	35.0	41.0
20	38.36074965435698	40.0	36.0	41.0	35.0	41.0
21	38.30337760483462	40.0	36.0	41.0	35.0	41.0
22	38.30835893072751	40.0	36.0	41.0	35.0	41.0
23	38.254202456098646	40.0	35.0	41.0	35.0	41.0
24	38.20373857501236	40.0	35.0	41.0	35.0	41.0
25	38.0688658529719	40.0	35.0	41.0	34.0	41.0
26	38.08850807334514	40.0	35.0	41.0	35.0	41.0
27	38.06593336127672	40.0	35.0	41.0	35.0	41.0
28	37.96708102122655	40.0	35.0	41.0	34.0	41.0
29	37.904541530338385	40.0	35.0	41.0	34.0	41.0
30	37.688502130161964	40.0	35.0	41.0	33.0	41.0
31	37.4588559685199	40.0	35.0	41.0	33.0	41.0
32	37.000403510857254	40.0	35.0	41.0	32.0	41.0
33	36.480623658873796	40.0	35.0	41.0	30.0	41.0
34	36.0411174435554	40.0	35.0	41.0	24.0	41.0
35	35.73154954425169	40.0	35.0	41.0	22.0	41.0
36	35.54409059913542	40.0	35.0	41.0	21.0	41.0
37	35.4390729885453	40.0	35.0	41.0	20.0	41.0
38	35.340994826302655	40.0	35.0	41.0	18.0	41.0
39	35.277196318980025	40.0	35.0	41.0	18.0	41.0
40	35.17118713519803	40.0	35.0	41.0	18.0	41.0
41	35.10755284741034	40.0	35.0	41.0	18.0	41.0
42	35.03686024761178	40.0	35.0	41.0	18.0	41.0
43	34.984682227379935	40.0	35.0	41.0	18.0	41.0
44	34.90246767221155	39.0	35.0	41.0	17.0	41.0
45	34.83554586573411	39.0	35.0	41.0	18.0	41.0
46	34.7418468910896	39.0	35.0	41.0	17.0	41.0
47	34.674671717329694	39.0	35.0	41.0	17.0	41.0
48	34.60957196569219	39.0	35.0	41.0	17.0	41.0
49	34.55952254343216	39.0	35.0	41.0	16.0	41.0
50	34.46094703059801	39.0	35.0	41.0	15.0	41.0
51	33.787728265153554	38.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	9.0
10	11.0
11	15.0
12	7.0
13	17.0
14	18.0
15	39.0
16	53.0
17	100.0
18	197.0
19	345.0
20	641.0
21	1042.0
22	1590.0
23	2644.0
24	4848.0
25	9452.0
26	15107.0
27	17401.0
28	15708.0
29	12744.0
30	10948.0
31	10396.0
32	11688.0
33	14237.0
34	22470.0
35	32591.0
36	36271.0
37	51213.0
38	104316.0
39	262853.0
40	415.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.06024510938585	19.331298053763913	22.83636852740433	22.772088309445905
2	41.165614618979404	21.69168016916176	22.650565853597502	14.492139358261339
3	27.5865358749304	21.42580092213179	36.65129780352462	14.33636539941319
4	24.759301081659338	23.33731630872021	35.807834992211305	16.09554761740915
5	22.89329796618016	26.808448078475045	33.95606423642608	16.342189718918714
6	23.417392881943357	30.11379631772883	33.27228537288782	13.19652542743999
7	73.00011886366337	4.632711280161654	18.863506978548237	3.5036628776267307
8	75.39616007807466	3.821623177163162	17.81406595056523	2.968150794196951
9	68.69991304184626	7.9890457750223645	19.446564527329258	3.8644766558021106
10	26.873979492890076	39.551414790393316	24.27524445250771	9.2993612642089
11	22.376241030485403	23.139158069904344	40.03093583238972	14.45366506722053
12	21.524176243532878	22.32838276602001	39.73909425888506	16.408346731562055
13	21.338529969283126	21.928469098575512	40.605235005974464	16.127765926166898
14	18.5907461510069	24.864557983571792	38.97993080883595	17.56476505658536
15	17.795610802830204	25.341263833540822	40.1360363347451	16.72708902888387
16	21.380132251465465	25.31139151813922	38.16289952266855	15.145576707726763
17	21.743917621225297	24.33170469261231	37.21981019349753	16.70456749266486
18	21.0617027532578	23.669352568393524	38.27409960774991	16.99484507059876
19	20.117675026744326	25.165627130943964	37.43892597296165	17.27777186935007
20	21.141153728252643	24.794021783330308	37.67289971034802	16.39192477806903
21	21.579385287180866	24.69126727433108	38.16477631735347	15.564571121134584
22	21.166959655170256	22.996208874736467	37.983352831144785	17.853478638948495
23	18.602163318673483	25.112764080652124	38.686994438431746	17.598078162242643
24	18.988939423323554	23.756154322570957	39.15197032162005	18.10293593248544
25	18.925441203150513	26.385230877026157	37.04730148204221	17.642026437781126
26	19.37149273993256	26.248068465470105	36.669752951259646	17.710685843337693
27	19.231671535906212	25.03472070167097	37.533860504107054	18.199747258315764
28	18.392587912191033	25.54630365286806	38.21263458181887	17.848473853122048
29	19.214311185070724	24.10492533485145	37.49257102103887	19.188192459038955
30	19.958147478526342	24.575375202537426	36.78580142261037	18.680675896325862
31	20.272197789135863	25.468573073001053	35.47392193785307	18.78530720001001
32	20.14113496030579	26.633593373663565	35.016140434290286	18.209131231740354
33	20.059337991954806	26.603721058261964	33.66203306912235	19.674907880660882
34	19.97191063954907	27.52303765475736	33.44166609320162	19.063385612491945
35	20.228405913154454	27.93702728233874	32.23238471788648	19.60218208662033
36	20.335070411080597	28.899040957916007	31.47056247536707	19.295326155636328
37	20.181329646474442	28.910614525139668	31.46696528555431	19.44109054283158
38	19.91154041051756	29.08812802242144	31.63368721339781	19.36664435366319
39	20.642239141178752	27.99692831269902	31.39580348708452	19.965029059037708
40	21.33086639098638	27.81409723047664	31.74660769360701	19.108428684929965
41	20.26922619755141	27.859765901142968	31.401121072025123	20.4698868292805
42	20.64599273054859	27.54024160603577	31.301025355496193	20.512740307919447
43	20.532759451225235	27.501767314994964	31.36858996415322	20.59688326962658
44	20.878715271478352	27.641119320350082	31.127891045812557	20.352274362359005
45	20.838364185752624	27.118275601043496	31.17934650009071	20.864013713113163
46	20.570138945366505	27.586379475373324	30.999487009452793	20.84399456980738
47	20.226216319355384	27.39338242194098	31.84639061102179	20.534010647681846
48	20.45002408553179	27.122654788641636	31.647606773977614	20.779714351848956
49	20.85853972861549	26.46968663784744	31.538596282695323	21.13317735084174
50	19.97316183600568	26.690992011110627	32.09240711430305	21.243439038580643
51	20.47332761953618	26.15125713963978	31.38016353137688	21.995251709447157
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	166.0
1	383.0
2	600.0
3	11942.5
4	23285.0
5	15826.0
6	8367.0
7	8269.0
8	8171.0
9	8140.5
10	8110.0
11	8064.0
12	8018.0
13	7734.0
14	7450.0
15	7139.5
16	6829.0
17	6411.0
18	5993.0
19	5699.5
20	5406.0
21	5410.0
22	5414.0
23	5505.0
24	5596.0
25	5951.0
26	7027.0
27	7748.0
28	8943.0
29	10138.0
30	11576.5
31	13015.0
32	14720.5
33	16426.0
34	18610.0
35	20794.0
36	22715.5
37	24637.0
38	26522.5
39	28408.0
40	30718.5
41	33029.0
42	34937.0
43	36845.0
44	38563.5
45	40282.0
46	44625.0
47	48968.0
48	50418.5
49	51869.0
50	50472.5
51	49076.0
52	44224.0
53	39372.0
54	35189.0
55	31006.0
56	27362.5
57	23719.0
58	21624.5
59	19530.0
60	17322.5
61	15115.0
62	12958.0
63	10801.0
64	9102.5
65	7404.0
66	5860.5
67	4317.0
68	3575.5
69	2834.0
70	2359.5
71	1885.0
72	1428.0
73	971.0
74	808.5
75	548.0
76	450.0
77	327.5
78	205.0
79	167.5
80	130.0
81	81.5
82	33.0
83	21.0
84	9.0
85	6.5
86	4.0
87	7.5
88	11.0
89	5.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	639388.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.68425033288227
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.9555712114552	27.869602590437793
2	7.814615566096325	5.88975858496025
3	3.441227744248955	3.890402633002122
4	2.2484924540260596	3.389310100364593
5	1.6608780019213207	3.129447119839019
6	1.3790996815903815	3.118220258103011
7	1.145095763678202	3.0206452789500657
8	1.0013317973270692	3.018755049339857
9	0.8587946657937587	2.912672985328438
>10	6.446131607614284	39.057866210894545
>50	0.032924435868674884	0.8281498020304034
>100	0.012919715309850748	1.0828926368501557
>500	0.0025005900599711123	0.6203663401824677
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.1676500999518544E-4	2.1719104097173028
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13829	2.162849474810287	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCC	847	0.13247042484375685	No Hit
CGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTG	666	0.10416210501291862	Illumina Single End Adapter 1 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.691986712293631E-4	0.0	0.0	0.09884452007231916	0.0
2	4.691986712293631E-4	0.0	0.0	0.3483018136092639	0.0
3	4.691986712293631E-4	0.0	0.0	0.5847779439088628	0.0
4	4.691986712293631E-4	0.0	0.0	0.7801209906973543	0.0
5	4.691986712293631E-4	0.0	0.0	1.2652724167485159	0.0
6	4.691986712293631E-4	0.0	0.0	1.8397279898903327	0.0
7	4.691986712293631E-4	0.0	0.0	2.3459933561468156	0.0
8	4.691986712293631E-4	0.0	0.0	3.112507585378518	0.0
9	4.691986712293631E-4	0.0	0.0	3.547141954493985	0.0
10	4.691986712293631E-4	0.0	0.0	4.207930083142005	0.0
11	4.691986712293631E-4	0.0	0.0	4.895618935607175	0.0
12	4.691986712293631E-4	0.0	0.0	5.474766495461285	0.0
13	4.691986712293631E-4	0.0	0.0	5.779276433089142	0.0
14	4.691986712293631E-4	0.0	0.0	5.950221148973706	0.0
15	4.691986712293631E-4	0.0	0.0	6.14149780727821	0.0
16	4.691986712293631E-4	0.0	0.0	6.4397517626230085	0.0
17	4.691986712293631E-4	0.0	0.0	6.801973136812077	0.0
18	4.691986712293631E-4	0.0	0.0	7.203607199384411	0.0
19	4.691986712293631E-4	0.0	0.0	7.4715196406563775	0.0
20	4.691986712293631E-4	0.0	0.0	7.7317685036315975	0.0
21	4.691986712293631E-4	0.0	0.0	8.024079275807491	0.0
22	4.691986712293631E-4	0.0	0.0	8.357366731937415	0.0
23	4.691986712293631E-4	0.0	0.0	8.703791750861761	0.0
24	4.691986712293631E-4	0.0	0.0	8.974988582832333	0.0
25	6.255982283058174E-4	0.0	0.0	9.23273505289433	0.0
26	6.255982283058174E-4	0.0	0.0	9.498145101253073	0.0
27	6.255982283058174E-4	0.0	0.0	9.768090736767034	0.0
28	6.255982283058174E-4	0.0	0.0	10.030841992655477	0.0
29	6.255982283058174E-4	0.0	0.0	10.321588769260606	0.0
30	6.255982283058174E-4	0.0	0.0	10.661601406344817	0.0
31	6.255982283058174E-4	0.0	0.0	10.991135273104907	0.0
32	6.255982283058174E-4	0.0	0.0	11.304090786814891	0.0
33	6.255982283058174E-4	0.0	0.0	11.599529550132313	0.0
34	6.255982283058174E-4	0.0	0.0	11.904352286874323	0.0
35	6.255982283058174E-4	0.0	0.0	12.226535374451819	0.0
36	6.255982283058174E-4	0.0	0.0	12.527291722709842	0.0
37	6.255982283058174E-4	0.0	0.0	12.840247236419826	0.0
38	6.255982283058174E-4	0.0	0.0	13.153359149686889	0.0
39	6.255982283058174E-4	0.0	0.0	13.489148998730036	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATG	30	2.1639844E-6	45.000004	1
ACGGGTC	30	2.1639844E-6	45.000004	5
TTAAGCG	25	3.8888567E-5	45.0	1
TGTTACG	25	3.8888567E-5	45.0	1
GCGAACG	55	1.8189894E-12	44.999996	1
CGTTTTT	9295	0.0	44.394836	1
CGAACGG	80	0.0	42.1875	2
GACGTAG	55	6.002665E-11	40.90909	1
ACGCGAG	50	1.0804797E-9	40.5	1
CGGCGTC	40	3.4550067E-7	39.375	5
TCGGCGT	40	3.4550067E-7	39.375	4
TCACGAC	35	6.2439394E-6	38.57143	25
CTCACGA	35	6.2439394E-6	38.57143	24
CGACGGT	35	6.2439394E-6	38.57143	28
TAGACGG	70	0.0	38.57143	2
GTGCTCG	35	6.2439394E-6	38.57143	1
TTTGACG	65	9.094947E-12	38.07692	1
GTTTTTT	11020	0.0	37.833485	2
GTTTGCG	30	1.1393552E-4	37.500004	1
CAACGCG	30	1.1393552E-4	37.500004	1
>>END_MODULE