FastQCFastQC Report
Sat 18 Jun 2016
SRR3552066_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552066_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197395
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22561.1428860913396997No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCG12350.6256490792573266No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTC10560.53496795764837No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGC10170.5152106183034018No Hit
GCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC8850.4483396235973556RNA PCR Primer, Index 24 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC5780.29281390106132377RNA PCR Primer, Index 3 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGCT4960.25127282859241623TruSeq Adapter, Index 3 (95% over 24bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTGCATGT4300.21783733123939308No Hit
TCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC3600.18237544010739887RNA PCR Primer, Index 24 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCC2900.14691354897540465No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCT2630.1332353909673497No Hit
ACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC2180.11043846095392486TruSeq Adapter, Index 3 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGA253.879032E-545.024
CGAACGG351.2050987E-745.02
ATTAGCG253.879032E-545.01
GTCGAAT207.019076E-445.043
GCGAACG207.019076E-445.01
TAGCGCG207.019076E-445.01
CACGACG253.879032E-545.026
CGACAGG351.2050987E-745.02
ATGTCGA207.019076E-445.041
TATGATT207.019076E-445.012
ATGTAAG207.019076E-445.023
CGCCTGG253.879032E-545.02
GCCGATT207.019076E-445.09
GGGCGCA207.019076E-445.01
GGAACGT207.019076E-445.08
GACGGTC253.879032E-545.029
TTAGTGA207.019076E-445.014
CGTAAGG253.879032E-545.02
CGGTCTA253.879032E-545.031
CACAACG253.879032E-545.012
Produced by FastQC (version 0.11.5)