##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552066_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	197395
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25264571037767	33.0	31.0	34.0	30.0	34.0
2	32.39470098026799	34.0	31.0	34.0	30.0	34.0
3	32.408171432913704	34.0	31.0	34.0	30.0	34.0
4	35.9186605537121	37.0	35.0	37.0	35.0	37.0
5	35.97799842954482	37.0	35.0	37.0	35.0	37.0
6	36.0055016591099	37.0	35.0	37.0	35.0	37.0
7	36.161726487499685	37.0	36.0	37.0	35.0	37.0
8	35.85006205830948	37.0	36.0	37.0	35.0	37.0
9	37.67227640011145	39.0	38.0	39.0	35.0	39.0
10	37.54081916968515	39.0	37.0	39.0	35.0	39.0
11	37.61050178575952	39.0	37.0	39.0	35.0	39.0
12	37.421277134679194	39.0	37.0	39.0	35.0	39.0
13	37.333772385318774	39.0	37.0	39.0	34.0	39.0
14	38.56145292433952	40.0	38.0	41.0	34.0	41.0
15	38.67155703031992	40.0	38.0	41.0	35.0	41.0
16	38.78243623192077	40.0	38.0	41.0	35.0	41.0
17	38.73024139415892	40.0	38.0	41.0	35.0	41.0
18	38.60085108538717	40.0	38.0	41.0	35.0	41.0
19	38.46829960232022	40.0	37.0	41.0	35.0	41.0
20	38.2656804883609	40.0	36.0	41.0	34.0	41.0
21	38.27329972896983	40.0	36.0	41.0	34.0	41.0
22	38.26306644038603	40.0	36.0	41.0	34.0	41.0
23	38.16358570379189	40.0	36.0	41.0	34.0	41.0
24	38.15488234251121	40.0	36.0	41.0	34.0	41.0
25	38.09394868157755	40.0	36.0	41.0	34.0	41.0
26	38.07758048582791	40.0	36.0	41.0	34.0	41.0
27	38.01262949922744	40.0	36.0	41.0	34.0	41.0
28	37.925590820436184	40.0	36.0	41.0	34.0	41.0
29	37.93629017958915	40.0	36.0	41.0	34.0	41.0
30	37.83006155171103	40.0	36.0	41.0	34.0	41.0
31	37.72105676435574	40.0	36.0	41.0	33.0	41.0
32	37.435005952531725	40.0	35.0	41.0	33.0	41.0
33	37.167532105676436	40.0	35.0	41.0	32.0	41.0
34	36.918214747080725	40.0	35.0	41.0	31.0	41.0
35	36.78912839737582	40.0	35.0	41.0	31.0	41.0
36	36.61029914638162	40.0	35.0	41.0	30.0	41.0
37	36.507581245725575	40.0	35.0	41.0	30.0	41.0
38	36.48217026773728	40.0	35.0	41.0	30.0	41.0
39	36.45836520681881	40.0	35.0	41.0	30.0	41.0
40	36.404437802375945	40.0	35.0	41.0	30.0	41.0
41	36.34958838876365	40.0	35.0	41.0	29.0	41.0
42	36.30635021150485	40.0	35.0	41.0	30.0	41.0
43	36.2344588262114	40.0	35.0	41.0	29.0	41.0
44	36.1571569695281	39.0	35.0	41.0	29.0	41.0
45	36.128123812659894	39.0	35.0	41.0	29.0	41.0
46	36.08002229033157	39.0	35.0	41.0	29.0	41.0
47	35.971833126472305	39.0	35.0	41.0	28.0	41.0
48	35.98661060310545	39.0	35.0	41.0	28.0	41.0
49	35.97024240735581	39.0	35.0	41.0	28.0	41.0
50	35.885503685503686	39.0	35.0	41.0	28.0	41.0
51	35.13453734897034	38.0	35.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	1.0
11	3.0
12	6.0
13	3.0
14	9.0
15	9.0
16	10.0
17	22.0
18	49.0
19	57.0
20	179.0
21	386.0
22	658.0
23	958.0
24	1238.0
25	1873.0
26	2614.0
27	3059.0
28	2746.0
29	2490.0
30	2565.0
31	2868.0
32	3714.0
33	5075.0
34	9481.0
35	15228.0
36	11668.0
37	16816.0
38	32480.0
39	80967.0
40	157.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.813141163656624	18.319106360343472	24.028977431039287	14.838775044960611
2	34.95731908103042	25.826388712986653	23.855214164492512	15.361078041490412
3	28.602548190177057	26.131867575166545	30.0017730945566	15.2638111400998
4	27.152663441323234	22.480305985460625	32.933458294283035	17.433572278933106
5	26.668861926593884	26.170369056966997	28.76972567694217	18.39104333949695
6	22.910914663491983	34.10471389852833	28.615213151295627	14.369158286684062
7	73.92588464753413	5.321310063578104	16.449758099242633	4.3030471896451274
8	74.25517363661693	7.388231718128625	13.066187086805645	5.290407558448795
9	67.91408090377162	7.970819929582816	15.264824336989285	8.850274829656273
10	32.526153144709845	33.789609665898325	21.30702398743636	12.37721320195547
11	31.330074216672156	22.869373591023077	29.62435725322323	16.176194939081537
12	27.01740165657692	21.023835456825147	32.70143620659084	19.257326680007093
13	25.03406874540895	23.375465437321107	34.99126117682819	16.59920464044175
14	19.285189594467944	28.735783581144407	31.84984422097824	20.129182603409408
15	17.088072139618532	24.428683603941337	39.525823855720766	18.957420400719368
16	19.86625801058791	24.868411053978065	34.158413333671064	21.106917601762962
17	20.435674662478785	24.274677676739532	33.061121102358214	22.228526558423464
18	20.940753311887335	23.34152334152334	35.419843461080575	20.297879885508753
19	20.520783201195574	25.391220648952604	32.509941994478076	21.578054155373742
20	22.778185870969374	25.012285012285012	33.90055472529699	18.30897439144862
21	21.705716963448925	26.61718888523012	33.78707667367461	17.890017477646346
22	20.39970617290205	23.40130195800299	33.71716608830011	22.481825780794853
23	19.81559816611363	26.239773043896754	32.876719268471845	21.06790952151777
24	20.123103422072493	24.478330251526128	33.717672686744855	21.680893639656524
25	18.09063046176448	27.722586691658858	32.9780389574204	21.20874388915626
26	18.13369132956762	26.288913093036804	35.2009929329517	20.376402644443882
27	21.69051901010664	25.514830669469845	32.66445452012462	20.130195800298893
28	17.094151320955444	26.3907393804301	36.1544112059576	20.360698092656857
29	20.331315382861774	23.009701360216823	35.77750196306898	20.881481293852428
30	20.586640999012133	26.45051799690975	32.644190582334915	20.318650421743207
31	21.514222751336153	24.91349831556017	31.630487094404618	21.941791838699054
32	24.092808835076877	24.696167582765522	32.25360318143824	18.957420400719368
33	23.4843841029408	24.176904176904177	30.044833962359736	22.293877757795283
34	20.323716406190634	24.92616327667874	34.04898806960663	20.701132247524
35	22.407862407862407	23.734137136198992	32.58137237518681	21.27662808075179
36	22.42863294409686	25.658198029332052	32.1608956660503	19.752273360520782
37	22.783251855416804	25.380582081613007	32.77995896552597	19.05620709744421
38	21.05575115884394	25.68758073912713	30.829554953266292	22.427113148762633
39	23.184477823653083	25.880594746574126	30.45467210415664	20.480255325616152
40	21.35464424124218	24.338509080777122	31.875680741660123	22.43116593632058
41	20.07497656982193	25.88667392791104	30.796119455913267	23.242230046353757
42	21.28169406519922	23.5826642012209	33.39800906811216	21.737632665467718
43	24.422604422604422	23.178905240760912	30.27330986093873	22.12518047569594
44	21.611996251171508	23.965146027001698	31.452671040299908	22.97018668152689
45	20.416930520023303	24.346108057448262	31.351351351351354	23.885610071177084
46	22.35314977583019	25.968236277514627	30.912637098204108	20.765976848451075
47	19.772537298310493	25.040654525190607	33.749081790318904	21.437726386179996
48	21.01015729881709	24.041135793713114	32.271840725448975	22.67686618202082
49	21.783226525494566	22.084652600116517	33.811393399022265	22.32072747536665
50	21.200638314040376	23.148509334076344	32.16849464272145	23.482357709161832
51	20.046607056916336	22.918007041718383	30.966336533346844	26.069049368018437
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	85.0
1	208.5
2	332.0
3	2081.0
4	3830.0
5	2540.0
6	1250.0
7	1251.5
8	1253.0
9	1246.5
10	1240.0
11	1237.0
12	1234.0
13	1191.0
14	1148.0
15	1070.5
16	993.0
17	934.0
18	875.0
19	831.5
20	788.0
21	980.5
22	1173.0
23	1033.5
24	894.0
25	1170.5
26	1597.5
27	1748.0
28	2313.5
29	2879.0
30	3424.5
31	3970.0
32	4183.0
33	4396.0
34	4920.5
35	5445.0
36	5950.0
37	6455.0
38	6905.5
39	7356.0
40	8578.0
41	9800.0
42	10807.0
43	11814.0
44	12665.0
45	13516.0
46	16813.5
47	20111.0
48	20015.0
49	19919.0
50	19661.5
51	19404.0
52	16524.5
53	13645.0
54	12081.5
55	10518.0
56	9187.0
57	7856.0
58	7065.5
59	6275.0
60	5712.0
61	5149.0
62	4237.0
63	3325.0
64	2977.0
65	2629.0
66	2192.0
67	1755.0
68	1432.5
69	1110.0
70	903.5
71	697.0
72	613.5
73	530.0
74	384.5
75	201.0
76	163.0
77	122.5
78	82.0
79	64.5
80	47.0
81	30.5
82	14.0
83	9.0
84	4.0
85	3.0
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	197395.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.52118847995137
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.23269201425975	27.475873249069128
2	9.18699976418693	6.710402999062792
3	3.2001220679419067	3.5061678360647432
4	1.7255968151364247	2.5208338610400465
5	1.1832267550734488	2.160642366827934
6	0.889153985934444	1.9483776184807111
7	0.7726345868416308	1.9752273360520785
8	0.7129877515917382	2.0831328047822897
9	0.606178302423326	1.9924516831733328
>10	6.419664035732615	43.303528458167634
>50	0.03883979969760441	0.975708604574584
>100	0.023581306959259824	1.7877859114972516
>500	0.002774271406971744	0.7411535246586793
>1k	0.005548542813943488	2.818713746548798
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2256	1.1428860913396997	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCG	1235	0.6256490792573266	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTC	1056	0.53496795764837	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGC	1017	0.5152106183034018	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	885	0.4483396235973556	RNA PCR Primer, Index 24 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	578	0.29281390106132377	RNA PCR Primer, Index 3 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGCT	496	0.25127282859241623	TruSeq Adapter, Index 3 (95% over 24bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTGCATGT	430	0.21783733123939308	No Hit
TCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	360	0.18237544010739887	RNA PCR Primer, Index 24 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCC	290	0.14691354897540465	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCT	263	0.1332353909673497	No Hit
ACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	218	0.11043846095392486	TruSeq Adapter, Index 3 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2583652068188151	0.0
2	0.0	0.0	0.0	1.319688948554928	0.0
3	5.065984447427746E-4	0.0	0.0	1.7209149167912055	0.0
4	5.065984447427746E-4	0.0	0.0	2.2335925428708934	0.0
5	5.065984447427746E-4	0.0	0.0	3.8268446515869194	0.0
6	5.065984447427746E-4	0.0	0.0	4.435775982167735	0.0
7	5.065984447427746E-4	0.0	0.0	5.1987132399503535	0.0
8	5.065984447427746E-4	0.0	0.0	6.500164644494541	0.0
9	5.065984447427746E-4	0.0	0.0	7.035639200587654	0.0
10	5.065984447427746E-4	0.0	0.0	8.87915094100661	0.0
11	5.065984447427746E-4	0.0	0.0	10.252539324704273	0.0
12	5.065984447427746E-4	0.0	0.0	11.93545935813977	0.0
13	5.065984447427746E-4	0.0	0.0	12.460295346893284	0.0
14	5.065984447427746E-4	0.0	0.0	12.670027103016794	0.0
15	5.065984447427746E-4	0.0	0.0	13.106208363940322	0.0
16	5.065984447427746E-4	0.0	0.0	13.911699891081334	0.0
17	5.065984447427746E-4	0.0	0.0	14.767851262696624	0.0
18	5.065984447427746E-4	0.0	0.0	15.754705033055549	0.0
19	5.065984447427746E-4	0.0	0.0	16.534360039514677	0.0
20	5.065984447427746E-4	0.0	0.0	17.148863952987664	0.0
21	5.065984447427746E-4	0.0	0.0	17.791230780921502	0.0
22	5.065984447427746E-4	0.0	0.0	18.46652650776362	0.0
23	5.065984447427746E-4	0.0	0.0	19.086096405684035	0.0
24	5.065984447427746E-4	0.0	0.0	19.573950707971328	0.0
25	5.065984447427746E-4	0.0	0.0	20.03495529268725	0.0
26	5.065984447427746E-4	0.0	0.0	20.473669545834493	0.0
27	5.065984447427746E-4	0.0	0.0	20.930621342992477	0.0
28	5.065984447427746E-4	0.0	0.0	21.35312444590795	0.0
29	5.065984447427746E-4	0.0	0.0	21.8333797715241	0.0
30	5.065984447427746E-4	0.0	0.0	22.363788343169787	0.0
31	5.065984447427746E-4	0.0	0.0	22.84303047189645	0.0
32	5.065984447427746E-4	0.0	0.0	23.30454165505712	0.0
33	5.065984447427746E-4	0.0	0.0	23.790876162010182	0.0
34	5.065984447427746E-4	0.0	0.0	24.23769599027331	0.0
35	5.065984447427746E-4	0.0	0.0	24.66881126674941	0.0
36	5.065984447427746E-4	0.0	0.0	25.11968388257048	0.0
37	5.065984447427746E-4	0.0	0.0	25.55434534815978	0.0
38	5.065984447427746E-4	0.0	0.0	25.99103320752805	0.0
39	5.065984447427746E-4	0.0	0.0	26.440386028014895	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGA	25	3.879032E-5	45.0	24
CGAACGG	35	1.2050987E-7	45.0	2
ATTAGCG	25	3.879032E-5	45.0	1
GTCGAAT	20	7.019076E-4	45.0	43
GCGAACG	20	7.019076E-4	45.0	1
TAGCGCG	20	7.019076E-4	45.0	1
CACGACG	25	3.879032E-5	45.0	26
CGACAGG	35	1.2050987E-7	45.0	2
ATGTCGA	20	7.019076E-4	45.0	41
TATGATT	20	7.019076E-4	45.0	12
ATGTAAG	20	7.019076E-4	45.0	23
CGCCTGG	25	3.879032E-5	45.0	2
GCCGATT	20	7.019076E-4	45.0	9
GGGCGCA	20	7.019076E-4	45.0	1
GGAACGT	20	7.019076E-4	45.0	8
GACGGTC	25	3.879032E-5	45.0	29
TTAGTGA	20	7.019076E-4	45.0	14
CGTAAGG	25	3.879032E-5	45.0	2
CGGTCTA	25	3.879032E-5	45.0	31
CACAACG	25	3.879032E-5	45.0	12
>>END_MODULE