FastQCFastQC Report
Sat 18 Jun 2016
SRR3552065_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552065_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461094
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38020.8245607186387158No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA7390.16027100764703076No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG453.8380676E-1045.01
GTATTCG207.0288865E-445.01
CGTAAGG700.041.7857132
CGTTTTT16300.040.720861
TCGGCGT501.0786607E-940.54
GCGTCCC501.0786607E-940.57
CGACGGT403.4522236E-739.37528
GTTAGCG403.4522236E-739.3751
TTAGCGG1200.039.3749962
ACGTAGG700.038.5714262
GACGCAC356.240154E-638.5714269
GTAATGG1000.038.252
TAAGGGA4400.037.8409084
CGAAAGG601.546141E-1037.4999962
GTATGCG301.13886184E-437.4999961
AAACCGG301.13886184E-437.4999962
CATACGG301.13886184E-437.4999962
CTCGATC301.13886184E-437.49999616
CATCGTT301.13886184E-437.49999636
CGCACCC301.13886184E-437.49999630