##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552065_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	461094
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39579348245694	33.0	31.0	34.0	31.0	34.0
2	32.53483671442265	34.0	31.0	34.0	31.0	34.0
3	32.559488954529876	34.0	31.0	34.0	31.0	34.0
4	36.00121233414445	37.0	35.0	37.0	35.0	37.0
5	36.0576910564874	37.0	35.0	37.0	35.0	37.0
6	36.0705083995888	37.0	36.0	37.0	35.0	37.0
7	36.17848638238624	37.0	37.0	37.0	35.0	37.0
8	35.936754761501994	37.0	37.0	37.0	35.0	37.0
9	37.71147314864214	39.0	38.0	39.0	35.0	39.0
10	37.59308947850113	39.0	37.0	39.0	35.0	39.0
11	37.62701314699389	39.0	37.0	39.0	35.0	39.0
12	37.56187024771522	39.0	37.0	39.0	35.0	39.0
13	37.548187571297824	39.0	37.0	39.0	35.0	39.0
14	38.858807531652985	40.0	38.0	41.0	35.0	41.0
15	38.96209883451097	40.0	38.0	41.0	35.0	41.0
16	39.01571046250873	40.0	38.0	41.0	35.0	41.0
17	38.98860102278494	40.0	38.0	41.0	35.0	41.0
18	38.8974460739025	40.0	38.0	41.0	35.0	41.0
19	38.82491856324307	40.0	38.0	41.0	35.0	41.0
20	38.74409990153851	40.0	38.0	41.0	35.0	41.0
21	38.69222978394861	40.0	38.0	41.0	35.0	41.0
22	38.68876411317432	40.0	38.0	41.0	35.0	41.0
23	38.653406897508965	40.0	38.0	41.0	35.0	41.0
24	38.603941929411356	40.0	38.0	41.0	35.0	41.0
25	38.480338065557135	40.0	38.0	41.0	35.0	41.0
26	38.490357714479046	40.0	38.0	41.0	35.0	41.0
27	38.468425093364914	40.0	38.0	41.0	35.0	41.0
28	38.407110046975234	40.0	37.0	41.0	35.0	41.0
29	38.37226032002152	40.0	37.0	41.0	35.0	41.0
30	38.256531206218256	40.0	37.0	41.0	34.0	41.0
31	38.200599010180134	40.0	37.0	41.0	34.0	41.0
32	38.06580436960793	40.0	37.0	41.0	34.0	41.0
33	37.95104035185884	40.0	37.0	41.0	34.0	41.0
34	37.82975705604497	40.0	37.0	41.0	34.0	41.0
35	37.716712861151954	40.0	37.0	41.0	33.0	41.0
36	37.61510451231202	40.0	37.0	41.0	33.0	41.0
37	37.57346875040664	40.0	37.0	41.0	33.0	41.0
38	37.4822552451344	40.0	36.0	41.0	33.0	41.0
39	37.43102491032197	40.0	36.0	41.0	33.0	41.0
40	37.32053117151817	40.0	36.0	41.0	33.0	41.0
41	37.246377094475314	40.0	36.0	41.0	33.0	41.0
42	37.21181798071543	40.0	35.0	41.0	33.0	41.0
43	37.14479043318716	40.0	35.0	41.0	32.0	41.0
44	37.06676295939656	40.0	35.0	41.0	32.0	41.0
45	36.98588140379185	40.0	35.0	41.0	32.0	41.0
46	36.908652031906726	40.0	35.0	41.0	32.0	41.0
47	36.836495378382715	39.0	35.0	41.0	32.0	41.0
48	36.76829019679285	39.0	35.0	41.0	31.0	41.0
49	36.70218870772554	39.0	35.0	41.0	31.0	41.0
50	36.604553518371524	39.0	35.0	41.0	31.0	41.0
51	35.823090302628096	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	6.0
10	8.0
11	11.0
12	10.0
13	17.0
14	15.0
15	27.0
16	43.0
17	67.0
18	98.0
19	162.0
20	262.0
21	431.0
22	662.0
23	1016.0
24	1500.0
25	2492.0
26	3728.0
27	4463.0
28	4728.0
29	4789.0
30	5188.0
31	6012.0
32	7697.0
33	10835.0
34	19676.0
35	29138.0
36	29175.0
37	42723.0
38	81964.0
39	203804.0
40	344.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.55029993884111	21.03757585221235	24.455750888105246	13.956373320841303
2	33.05876892781082	24.392423236910478	25.065387968613773	17.48341986666493
3	32.00366953376101	23.428845311368182	27.961760508703215	16.605724646167594
4	28.743379874819453	25.398725639457464	27.466199950552383	18.391694535170704
5	25.8741167744538	29.61890634013889	25.389833743228063	19.117143142179252
6	26.393967390597144	33.624380278207916	24.61255188746763	15.36910044372731
7	80.05352487779065	6.2720399744954385	9.438856285269381	4.235578862444534
8	81.9928257578715	5.376994712574876	8.81208603885542	3.8180934906982094
9	74.37116943616702	9.600428546023155	10.773291346233089	5.255110671576729
10	34.734999804812034	35.08720564570348	16.992413694387697	13.185380855096792
11	30.874398712626927	25.892984944501556	25.55552663881985	17.677089704051667
12	28.812346289476764	23.90879083223811	27.513261937912876	19.765600940372245
13	25.565719788155995	23.94284028853119	29.97479906483277	20.516640858480052
14	22.044962632348284	26.677206816831273	29.43434527449934	21.843485276321097
15	21.213461897140277	26.1625612131149	31.36171800110173	21.262258888643096
16	24.61732314885902	26.051954699041847	30.009716023197004	19.321006128902134
17	24.108966935158556	25.63620433143784	28.698269767119065	21.55655896628453
18	24.107231931016234	25.017675354699907	29.25065171093096	21.624441003352896
19	24.694313957674574	25.826187285022144	28.445392913375585	21.0341058439277
20	26.18077875660928	26.02788151656712	28.362763341097473	19.42857638572612
21	25.55682789192659	25.922480014921035	28.76571805315185	19.75497404000052
22	25.40219564774211	24.229332847532174	28.93184469977922	21.436626804946496
23	23.358794519121915	26.073859126338668	29.57509748554525	20.992248868994174
24	22.442278580940112	25.00466282363249	30.744056526434953	21.809002068992438
25	22.75306119793361	26.771330791552266	28.63168898315745	21.84391902735668
26	22.83113638433812	27.758981899569285	28.020967525059966	21.388914191032633
27	22.70903546782218	26.990158189002678	29.01187176584384	21.288934577331304
28	21.346623465063523	27.677219829362343	29.426320880341102	21.549835825233032
29	22.453989858900787	26.618650427027895	29.06956065357606	21.857799060495257
30	22.875812741002918	26.10725795607837	29.15522648310323	21.86170281981548
31	23.36204765188877	26.787813330904324	27.963712388363327	21.886426628843576
32	23.317805046259547	28.18535916754501	28.11704337944107	20.379792406754373
33	23.169028441055403	27.471188087461556	27.57658958910764	21.783193882375393
34	22.16359354057958	27.481164361279912	28.93835096531293	21.41689113282758
35	22.729421766494468	27.360147822352925	28.3399914117295	21.57043899942311
36	22.108507159060842	29.123128906470264	27.07799277370775	21.690371160761146
37	23.160570295861582	28.15326159091205	27.636230356499976	21.049937756726393
38	22.007876918806144	27.882384069191968	27.73555934364793	22.374179668353957
39	22.835907645729502	26.153018690332125	28.141984063986953	22.86908959995142
40	23.72010913176055	26.02462838380027	28.95071286982698	21.304549614612206
41	21.86734158327803	26.99341132176953	28.36710085145328	22.772146243499154
42	22.22323430797191	27.649242887567393	27.956772371794038	22.170750432666658
43	22.615995870690142	26.95806061236971	28.570096336105	21.855847180835145
44	22.338828958954142	27.39853478900181	27.677002953844553	22.5856332981995
45	21.725288119125384	26.734895704563495	27.96739927216576	23.572416904145356
46	21.452675593262978	28.428042871952357	27.469886834354817	22.64939470042985
47	22.15253288917227	27.388992266219038	28.46903234481472	21.989442499793967
48	21.535305165541082	27.055654595375344	28.762031169349417	22.647009069734153
49	21.901174164053316	26.719497542800386	28.579638858887773	22.799689434258525
50	20.699900671012852	27.014231371477397	28.920567172854124	23.365300784655624
51	21.509713854441827	26.23694951571697	28.067812636902673	24.18552399293853
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	128.0
1	408.0
2	688.0
3	3437.0
4	6186.0
5	4007.0
6	1828.0
7	1791.5
8	1755.0
9	1666.5
10	1578.0
11	1563.0
12	1548.0
13	1512.5
14	1477.0
15	1357.5
16	1238.0
17	1241.5
18	1245.0
19	1311.5
20	1378.0
21	2107.0
22	2836.0
23	2763.5
24	2691.0
25	3089.0
26	3844.5
27	4202.0
28	5349.0
29	6496.0
30	7520.5
31	8545.0
32	9424.5
33	10304.0
34	12086.5
35	13869.0
36	14626.5
37	15384.0
38	17266.0
39	19148.0
40	20959.5
41	22771.0
42	23501.0
43	24231.0
44	26164.0
45	28097.0
46	29793.5
47	31490.0
48	33132.5
49	34775.0
50	35287.5
51	35800.0
52	33604.0
53	31408.0
54	29521.0
55	27634.0
56	26429.5
57	25225.0
58	24218.0
59	23211.0
60	21913.0
61	20615.0
62	18754.0
63	16893.0
64	14676.0
65	12459.0
66	10187.5
67	7916.0
68	6237.5
69	4559.0
70	4022.5
71	3486.0
72	2704.0
73	1922.0
74	1568.5
75	989.5
76	764.0
77	571.0
78	378.0
79	265.5
80	153.0
81	94.0
82	35.0
83	26.5
84	18.0
85	16.5
86	15.0
87	7.5
88	0.0
89	6.0
90	12.0
91	6.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	461094.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.740508756973288
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.4666044634999	21.354387628274186
2	7.420606383898155	4.562264310525463
3	3.264486791422131	3.01055954396207
4	2.2701238888155792	2.791390531341983
5	1.9139405692981293	2.941775341541779
6	1.649313550835042	3.042044259146359
7	1.443929909459703	3.107099801834115
8	1.3187849697793779	3.243209672965418
9	1.1838494969993956	3.27529222465035
>10	9.995097932748065	49.185138069347055
>50	0.041965636421602164	0.8785425196425861
>100	0.029873842876394763	1.615398739452321
>500	7.112819732474943E-4	0.16158360428468668
>1k	7.112819732474943E-4	0.8313137530316357
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3802	0.8245607186387158	No Hit
CAAGGAAGGTGAAATAATCTATATCTTTTATTTTGTTTTGGTTTAATATAA	739	0.16027100764703076	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07720768433334635	0.0
2	0.0	0.0	0.0	0.29234819798132267	0.0
3	0.0	0.0	0.0	0.4229072596910825	0.0
4	0.0	0.0	0.0	0.5361162799776185	0.0
5	0.0	0.0	0.0	0.8475495235244874	0.0
6	0.0	0.0	0.0	1.1459702360039385	0.0
7	2.1687551779029873E-4	0.0	0.0	1.4103414921903126	0.0
8	2.1687551779029873E-4	0.0	0.0	1.8787926106173578	0.0
9	2.1687551779029873E-4	0.0	0.0	2.115837551562154	0.0
10	2.1687551779029873E-4	0.0	0.0	2.5576129813009927	0.0
11	2.1687551779029873E-4	0.0	0.0	3.1449118834771217	0.0
12	2.1687551779029873E-4	0.0	0.0	3.65912373615792	0.0
13	2.1687551779029873E-4	0.0	0.0	3.8874936563911047	0.0
14	2.1687551779029873E-4	0.0	0.0	4.000052050124269	0.0
15	2.1687551779029873E-4	0.0	0.0	4.170516207107444	0.0
16	2.1687551779029873E-4	0.0	0.0	4.482383201689894	0.0
17	2.1687551779029873E-4	0.0	0.0	4.825697146351937	0.0
18	2.1687551779029873E-4	0.0	0.0	5.214771825267733	0.0
19	2.1687551779029873E-4	0.0	0.0	5.467648679011221	0.0
20	2.1687551779029873E-4	0.0	0.0	5.717923026541226	0.0
21	2.1687551779029873E-4	0.0	0.0	6.019813747305322	0.0
22	2.1687551779029873E-4	0.0	0.0	6.329295111192078	0.0
23	2.1687551779029873E-4	0.0	0.0	6.627498948153739	0.0
24	4.3375103558059746E-4	0.0	0.0	6.899894598498354	0.0
25	4.3375103558059746E-4	0.0	0.0	7.136288912889779	0.0
26	4.3375103558059746E-4	0.0	0.0	7.378538866261543	0.0
27	4.3375103558059746E-4	0.0	0.0	7.608210039601469	0.0
28	4.3375103558059746E-4	0.0	0.0	7.855014378846829	0.0
29	4.3375103558059746E-4	0.0	0.0	8.100734340503237	0.0
30	4.3375103558059746E-4	0.0	0.0	8.403058812302914	0.0
31	4.3375103558059746E-4	0.0	0.0	8.666128815382546	0.0
32	4.3375103558059746E-4	0.0	0.0	8.953488876454692	0.0
33	4.3375103558059746E-4	0.0	0.0	9.21352262228526	0.0
34	4.3375103558059746E-4	0.0	0.0	9.462929467744104	0.0
35	4.3375103558059746E-4	0.0	0.0	9.740313254997897	0.0
36	4.3375103558059746E-4	0.0	0.0	10.030926448836897	0.0
37	4.3375103558059746E-4	0.0	0.0	10.305707729877206	0.0
38	4.3375103558059746E-4	0.0	0.0	10.610200956854785	0.0
39	4.3375103558059746E-4	0.0	0.0	10.91968232074154	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	45	3.8380676E-10	45.0	1
GTATTCG	20	7.0288865E-4	45.0	1
CGTAAGG	70	0.0	41.785713	2
CGTTTTT	1630	0.0	40.72086	1
TCGGCGT	50	1.0786607E-9	40.5	4
GCGTCCC	50	1.0786607E-9	40.5	7
CGACGGT	40	3.4522236E-7	39.375	28
GTTAGCG	40	3.4522236E-7	39.375	1
TTAGCGG	120	0.0	39.374996	2
ACGTAGG	70	0.0	38.571426	2
GACGCAC	35	6.240154E-6	38.571426	9
GTAATGG	100	0.0	38.25	2
TAAGGGA	440	0.0	37.840908	4
CGAAAGG	60	1.546141E-10	37.499996	2
GTATGCG	30	1.13886184E-4	37.499996	1
AAACCGG	30	1.13886184E-4	37.499996	2
CATACGG	30	1.13886184E-4	37.499996	2
CTCGATC	30	1.13886184E-4	37.499996	16
CATCGTT	30	1.13886184E-4	37.499996	36
CGCACCC	30	1.13886184E-4	37.499996	30
>>END_MODULE