##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552059_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	431366
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16953121015565	33.0	31.0	34.0	30.0	34.0
2	32.30376988450643	34.0	31.0	34.0	30.0	34.0
3	32.33852459396429	34.0	31.0	34.0	30.0	34.0
4	35.83482935604568	37.0	35.0	37.0	35.0	37.0
5	35.8879350713779	37.0	35.0	37.0	35.0	37.0
6	35.88694751093039	37.0	35.0	37.0	35.0	37.0
7	36.00724674638242	37.0	35.0	37.0	35.0	37.0
8	35.63748881460291	37.0	35.0	37.0	35.0	37.0
9	37.43960349216211	39.0	37.0	39.0	34.0	39.0
10	37.31962417065786	39.0	37.0	39.0	34.0	39.0
11	37.29615685983596	39.0	37.0	39.0	34.0	39.0
12	37.191000217912396	39.0	37.0	39.0	34.0	39.0
13	37.17010380975784	39.0	37.0	39.0	34.0	39.0
14	38.3607562951183	40.0	38.0	41.0	34.0	41.0
15	38.473127228386105	40.0	38.0	41.0	34.0	41.0
16	38.57259032932591	40.0	38.0	41.0	34.0	41.0
17	38.52748014447128	40.0	38.0	41.0	34.0	41.0
18	38.487690267661335	40.0	38.0	41.0	34.0	41.0
19	38.440023089441446	40.0	37.0	41.0	34.0	41.0
20	38.29081105140414	40.0	37.0	41.0	34.0	41.0
21	38.28178855078982	40.0	37.0	41.0	34.0	41.0
22	38.26471488248958	40.0	37.0	41.0	34.0	41.0
23	38.15041982910104	40.0	37.0	41.0	34.0	41.0
24	38.11179833366561	40.0	37.0	41.0	34.0	41.0
25	38.02469596583875	40.0	36.0	41.0	34.0	41.0
26	38.011034712981555	40.0	36.0	41.0	34.0	41.0
27	37.942160485527374	40.0	36.0	41.0	34.0	41.0
28	37.89466485536644	40.0	36.0	41.0	33.0	41.0
29	37.874190362708234	40.0	36.0	41.0	33.0	41.0
30	37.764745019310745	40.0	36.0	41.0	33.0	41.0
31	37.74787071767362	40.0	36.0	41.0	33.0	41.0
32	37.564854439153756	40.0	36.0	41.0	33.0	41.0
33	37.48310715262677	40.0	36.0	41.0	33.0	41.0
34	37.40307071025533	40.0	36.0	41.0	33.0	41.0
35	37.262721215858456	40.0	35.0	41.0	32.0	41.0
36	37.19000338459684	40.0	35.0	41.0	32.0	41.0
37	37.05706522999031	40.0	35.0	41.0	32.0	41.0
38	36.97742056629405	40.0	35.0	41.0	31.0	41.0
39	36.935198879837536	40.0	35.0	41.0	31.0	41.0
40	36.829937918148396	40.0	35.0	41.0	31.0	41.0
41	36.78789705261889	39.0	35.0	41.0	31.0	41.0
42	36.69189504967939	39.0	35.0	41.0	31.0	41.0
43	36.648080748134994	39.0	35.0	41.0	31.0	41.0
44	36.608893607748406	39.0	35.0	41.0	31.0	41.0
45	36.53195662152326	39.0	35.0	41.0	31.0	41.0
46	36.43360394653264	39.0	35.0	41.0	31.0	41.0
47	36.29949972876861	39.0	35.0	41.0	30.0	41.0
48	36.244782852612396	39.0	35.0	41.0	30.0	41.0
49	36.204411103332205	39.0	35.0	41.0	30.0	41.0
50	36.0981718540636	38.0	35.0	41.0	30.0	41.0
51	35.293602184687714	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	5.0
10	12.0
11	9.0
12	21.0
13	12.0
14	27.0
15	36.0
16	58.0
17	79.0
18	127.0
19	252.0
20	499.0
21	816.0
22	1310.0
23	1822.0
24	2387.0
25	3206.0
26	3759.0
27	3985.0
28	4355.0
29	4954.0
30	5547.0
31	6985.0
32	8964.0
33	12416.0
34	23272.0
35	33997.0
36	28465.0
37	42152.0
38	76499.0
39	165089.0
40	246.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.633791258467284	19.976307822127843	23.36020919590325	12.029691723501621
2	33.19199936944497	26.512057046684255	22.968430520717906	17.327513063152868
3	32.38757806595791	25.64944849617262	24.607641770561425	17.35533166730804
4	28.768609487071306	24.1481711585985	27.697593227097173	19.38562612723302
5	28.490887088922168	27.88768702215752	23.01039024865196	20.61103564026836
6	26.379223211843307	36.014892226091064	22.70114009912696	14.904744462938666
7	76.4200238312709	6.742766003811148	11.711864171028779	5.12534599388918
8	77.34684699304071	8.36945888178484	8.687518255958977	5.596175869215469
9	70.680350328955	8.175655939503809	11.190497164820593	9.953496566720604
10	41.557053638905245	24.103197748547636	18.423566066866652	15.916182545680465
11	32.40334194164584	24.45834859492867	23.80437957558083	19.33392988784466
12	27.680670242902778	22.335325454486448	28.25581988381096	21.728184418799813
13	26.10358720900581	23.952281821005826	29.6189314874144	20.325199482573964
14	21.260368225590334	28.553247126570014	28.17839143557907	22.007993212260583
15	20.278371498912755	24.76342595383039	32.71467848648248	22.243524060774376
16	22.41507211973127	24.368401774826946	29.74596977972302	23.470556325718764
17	22.464450142106703	25.184877806781248	28.370803447652342	23.979868603459707
18	23.52109345660066	23.577426130014885	29.12121029473811	23.780270118646346
19	23.975463991135136	25.675644348418746	27.128007307020024	23.220884353426094
20	26.7793474682752	24.548759058432978	28.766754913460957	19.905138559830863
21	24.733984597766163	26.6717821988752	27.5791323377364	21.015100865622234
22	23.735760351998074	23.74897418897178	28.56947464566053	23.945790813369623
23	23.7550015532054	26.322890538429082	27.518163230296317	22.403944678069205
24	22.92461621917351	26.06927759721443	28.265324573563984	22.74078161004808
25	22.28803382742265	27.680902064604073	27.37281102358554	22.658253084387738
26	21.479671555013606	25.950816707853658	28.809873749901477	23.75963798723126
27	23.207670516452385	26.233175540028654	28.586629451556217	21.97252449196274
28	20.257971189198965	28.037907484595447	29.38873253803035	22.315388788175238
29	22.17490483719162	25.61560252778383	28.953371383001908	23.256121252022645
30	21.853368137498087	27.055447114515285	28.99950390155923	22.091680846427394
31	23.831270892930828	26.847503048455373	25.190673349313574	24.130552709300225
32	24.032028486250656	28.146631862501913	26.153197053082533	21.6681425981649
33	23.303412879086437	26.429992164426498	26.751297042418738	23.51529791406833
34	22.021438870935587	26.41677832745279	26.927713357102785	24.63406944450884
35	22.54118312523472	25.745422680507968	27.63847869326743	24.07491550098988
36	22.41136297251058	28.026548221232083	26.643499951317445	22.918588854939888
37	22.223123751060584	27.91481016120881	27.66745640592907	22.194609681801534
38	20.948568037351112	28.967048863378196	25.965653296736413	24.118729802534276
39	23.51947070469161	26.607335765915717	26.798356847781236	23.074836681611437
40	22.939220986354975	25.153581877106678	28.851833477835527	23.055363658702817
41	21.093688422360593	25.861797174557104	26.559812317150634	26.484702085931666
42	21.6917884116968	26.137433177394602	27.519090517101485	24.651687893807114
43	22.309361423941617	26.01271310209891	26.87508983090925	24.802835643050216
44	22.017729723714897	25.91812984797133	28.375439881678204	23.688700546635573
45	22.383776190056704	25.251642456753657	27.112938896435974	25.251642456753657
46	22.56737897748084	26.922149636271755	27.6565607859683	22.85391060027911
47	21.377670006444642	26.741792352665716	29.753156252463103	22.127381388426535
48	21.93334662444421	25.66057593783469	28.01194345405062	24.39413398367048
49	22.576651845532563	24.446293866461428	29.78468400383897	23.19237028416704
50	21.26917745023947	25.130167885276077	28.455882012026912	25.144772652457544
51	21.207744699396798	25.373117028231245	26.96016839528382	26.458969877088133
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	121.0
1	453.0
2	785.0
3	1497.5
4	2210.0
5	1643.5
6	1077.0
7	1024.5
8	972.0
9	989.5
10	1007.0
11	984.0
12	961.0
13	1041.0
14	1121.0
15	1014.5
16	908.0
17	998.0
18	1088.0
19	1127.5
20	1167.0
21	1412.0
22	1657.0
23	1756.5
24	1856.0
25	1971.0
26	2483.5
27	2881.0
28	3663.0
29	4445.0
30	5080.5
31	5716.0
32	7059.0
33	8402.0
34	9089.0
35	9776.0
36	10599.0
37	11422.0
38	12665.5
39	13909.0
40	16246.5
41	18584.0
42	21454.0
43	24324.0
44	31637.5
45	38951.0
46	38615.0
47	38279.0
48	38693.0
49	39107.0
50	36196.5
51	33286.0
52	30576.5
53	27867.0
54	26978.0
55	26089.0
56	24241.0
57	22393.0
58	21758.0
59	21123.0
60	19828.0
61	18533.0
62	16693.0
63	14853.0
64	13233.5
65	11614.0
66	9764.5
67	7915.0
68	6707.0
69	5499.0
70	4597.0
71	3695.0
72	3124.5
73	2554.0
74	2044.0
75	1177.0
76	820.0
77	624.0
78	428.0
79	319.5
80	211.0
81	152.5
82	94.0
83	61.5
84	29.0
85	20.0
86	11.0
87	7.5
88	4.0
89	2.0
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	431366.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.785620631842214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.46938855466304	22.618007240972666
2	8.509354584723397	5.239315241342435
3	3.0479533935524974	2.8149941063232973
4	1.7144772252505234	2.111249817539844
5	1.1721076958513361	1.8042031432070966
6	0.951119600743292	1.7568484322395332
7	0.8072133049275285	1.7395393802132124
8	0.7411105330610768	1.825243817366453
9	0.6486164554007838	1.797125411838486
>10	8.812121863977618	52.35759716808418
>50	0.08637840607649691	1.808031467088656
>100	0.034096739240722464	1.907168787887608
>500	0.0022731159493814975	0.39118420465845377
>1k	0.0037885265823024957	1.829491781238076
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTC	1856	0.4302610775999963	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCG	1754	0.40661526406809995	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGC	1754	0.40661526406809995	No Hit
GCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	1414	0.32779588562844547	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1065	0.24689011187715304	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTAATGACT	589	0.13654298206163676	No Hit
CCTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGC	582	0.13492023015258503	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTAATGACTCGTATGCCGTCTTCTGCT	506	0.11730178085430933	TruSeq Adapter, Index 14 (95% over 22bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	444	0.10292883537413704	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12286550168534377	0.0
2	0.0	0.0	0.0	0.7425249092418039	0.0
3	0.0	0.0	0.0	0.9750420756387846	0.0
4	0.0	0.0	0.0	1.3084016820982647	0.0
5	0.0	0.0	0.0	2.4169730576818758	0.0
6	0.0	0.0	0.0	2.8595206854504065	0.0
7	0.0	0.0	0.0	3.3917833116193674	0.0
8	0.0	0.0	0.0	4.21196849079436	0.0
9	0.0	0.0	0.0	4.543473523643495	0.0
10	0.0	0.0	0.0	5.661781410681416	0.0
11	0.0	0.0	0.0	6.653746470514598	0.0
12	0.0	0.0	0.0	7.88425606097838	0.0
13	0.0	0.0	0.0	8.262357255787428	0.0
14	0.0	0.0	0.0	8.410491322913721	0.0
15	0.0	0.0	0.0	8.738055386840873	0.0
16	0.0	0.0	0.0	9.2916456095288	0.0
17	0.0	0.0	0.0	9.945846450577932	0.0
18	0.0	0.0	0.0	10.636675120431374	0.0
19	0.0	0.0	0.0	11.18725166100249	0.0
20	0.0	0.0	0.0	11.601285219511968	0.0
21	0.0	0.0	0.0	12.082315249695155	0.0
22	0.0	0.0	0.0	12.577950047059806	0.0
23	0.0	0.0	0.0	13.064775619775318	0.0
24	0.0	0.0	0.0	13.464436232804625	0.0
25	0.0	0.0	0.0	13.825382621717985	0.0
26	0.0	0.0	0.0	14.155496724359361	0.0
27	0.0	0.0	0.0	14.534757027674875	0.0
28	0.0	0.0	0.0	14.894080664679182	0.0
29	0.0	0.0	0.0	15.251549728073144	0.0
30	0.0	0.0	0.0	15.652833093011504	0.0
31	0.0	0.0	0.0	16.01007033470417	0.0
32	0.0	0.0	0.0	16.393967072045548	0.0
33	0.0	0.0	0.0	16.76256357710158	0.0
34	0.0	0.0	0.0	17.13927384170287	0.0
35	0.0	0.0	0.0	17.54217995855028	0.0
36	0.0	0.0	0.0	17.92190390526838	0.0
37	0.0	0.0	0.0	18.294209557545102	0.0
38	0.0	0.0	0.0	18.682974550613633	0.0
39	0.0	0.0	0.0	19.081707876837765	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	20	7.028382E-4	45.0	33
ACTCGAG	20	7.028382E-4	45.0	1
CGTAAGC	20	7.028382E-4	45.0	43
ACCCGTT	20	7.028382E-4	45.0	16
AGTACGG	50	2.1827873E-11	45.0	2
ATCCGGC	55	6.002665E-11	40.909092	6
GATGAAT	705	0.0	40.212765	20
CTCGAAT	90	0.0	40.0	43
CGATGAA	640	0.0	39.726562	19
TTATAGG	80	0.0	39.375	2
GAACGCC	40	3.4515324E-7	39.375	29
GCGTAAG	40	3.4515324E-7	39.375	1
ACGGGCG	110	0.0	38.863636	5
CCGATGA	645	0.0	38.72093	18
ATGAATG	705	0.0	38.61702	21
ACAACGG	35	6.2392173E-6	38.571426	2
ATGGGTA	70	0.0	38.571426	5
GCGAACC	35	6.2392173E-6	38.571426	33
TAGCATA	35	6.2392173E-6	38.571426	30
TAGTGCG	35	6.2392173E-6	38.571426	1
>>END_MODULE