FastQCFastQC Report
Sat 18 Jun 2016
SRR3552055_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552055_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459810
Sequences flagged as poor quality0
Sequence length42-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209484.555794784802418No Hit
CGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG7560.1644157369348209No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG7260.15789130292947087No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTT5800.12613905743676737No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCC4850.10547834975315892No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACGA302.193001E-644.90898524
CGTTAGG406.9230737E-944.9089852
AAGCACG207.0993195E-444.9089851
TGCGACG302.193001E-644.9089851
GCTTACG207.0993195E-444.9089851
GCGATAC207.0993195E-444.9089859
CGGTCTA302.193001E-644.90898531
CGTTTTT88050.044.143921
CCGCCCA451.9586878E-839.91909436
TCGGCGT1100.038.785034
GTCGGCG1100.038.785033
GCGATCA356.3274438E-638.4934129
ACGTAGG700.038.4934122
GTTTTTT103700.038.4779432
TCGCAAC301.15250994E-437.42415216
ATAGCGG601.5825208E-1037.4241522
TAACGCC301.15250994E-437.42415212
TATAGCG301.15250994E-437.4241521
ACAACGA850.036.98386813
CGTAGGG1650.036.7437133
Produced by FastQC (version 0.11.5)