##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552055_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	459810
Sequences flagged as poor quality	0
Sequence length	42-51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.132619995215414	30.0	28.0	33.0	25.0	33.0
2	28.812035405928537	31.0	28.0	33.0	16.0	33.0
3	28.33419455862204	31.0	26.0	33.0	16.0	34.0
4	32.7586111654814	35.0	32.0	35.0	28.0	37.0
5	34.1284943781127	35.0	33.0	35.0	32.0	37.0
6	34.106937648159025	35.0	35.0	37.0	30.0	37.0
7	34.792233748722296	35.0	35.0	37.0	32.0	37.0
8	34.649468258628566	35.0	35.0	37.0	32.0	37.0
9	36.65054044061678	39.0	37.0	39.0	32.0	39.0
10	36.098756007916315	37.0	35.0	39.0	31.0	39.0
11	36.48203605837194	38.0	35.0	39.0	32.0	39.0
12	36.57310845784128	39.0	35.0	39.0	32.0	39.0
13	36.66761923403145	39.0	35.0	39.0	33.0	39.0
14	37.477636415041	39.0	37.0	41.0	32.0	41.0
15	37.82218307561819	39.0	37.0	41.0	33.0	41.0
16	37.56116439398882	39.0	36.0	41.0	32.0	41.0
17	37.544514038407165	39.0	36.0	41.0	33.0	41.0
18	37.17992866618821	39.0	36.0	40.0	32.0	41.0
19	36.57000500206607	37.0	35.0	40.0	32.0	41.0
20	36.5725212587808	38.0	35.0	40.0	32.0	41.0
21	36.52510602205259	38.0	35.0	40.0	32.0	41.0
22	36.78413257649899	38.0	35.0	40.0	33.0	41.0
23	36.99420847741458	38.0	35.0	40.0	33.0	41.0
24	36.91023901176573	38.0	35.0	40.0	33.0	41.0
25	36.203479698136185	38.0	35.0	40.0	31.0	41.0
26	36.37642069550466	38.0	35.0	40.0	31.0	41.0
27	36.61962114786542	38.0	35.0	40.0	33.0	41.0
28	36.54708031578261	38.0	35.0	40.0	32.0	41.0
29	36.46789760988234	38.0	35.0	40.0	32.0	41.0
30	35.96954611687436	38.0	35.0	40.0	31.0	41.0
31	35.8541462778104	38.0	35.0	40.0	30.0	41.0
32	35.41411670037624	38.0	35.0	40.0	27.0	41.0
33	34.99112459494139	38.0	35.0	40.0	23.0	41.0
34	34.551121115243255	38.0	34.0	40.0	18.0	41.0
35	34.178976098823426	38.0	34.0	40.0	16.0	41.0
36	33.95516843913791	38.0	34.0	40.0	15.0	41.0
37	33.875200626345666	38.0	34.0	40.0	15.0	41.0
38	33.71430808377373	38.0	34.0	40.0	15.0	41.0
39	33.617735586437874	38.0	33.0	40.0	12.0	41.0
40	33.50961484091255	38.0	33.0	40.0	12.0	41.0
41	33.33842456666884	38.0	33.0	40.0	10.0	41.0
42	33.34659315804354	38.0	33.0	40.0	10.0	41.0
43	33.274913492909164	37.0	33.0	40.0	10.0	41.0
44	33.2069820834203	37.0	33.0	40.0	10.0	41.0
45	33.13948348401129	37.0	33.0	40.0	10.0	41.0
46	33.03913525864247	37.0	33.0	40.0	10.0	41.0
47	33.00248041918864	37.0	33.0	40.0	10.0	41.0
48	32.900198828996075	37.0	33.0	40.0	10.0	41.0
49	32.872681335339934	36.0	33.0	40.0	10.0	41.0
50	32.795821295574136	36.0	33.0	40.0	10.0	41.0
51	31.98931815934881	35.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	9.0
10	12.0
11	17.0
12	20.0
13	22.0
14	51.0
15	72.0
16	125.0
17	209.0
18	378.0
19	597.0
20	1048.0
21	1598.0
22	2250.0
23	3585.0
24	5763.0
25	9866.0
26	13733.0
27	15371.0
28	13963.0
29	12026.0
30	12036.0
31	13085.0
32	16369.0
33	23339.0
34	31651.0
35	40419.0
36	43289.0
37	66829.0
38	93027.0
39	39045.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.496422435353736	16.827820186598814	18.381070442139144	30.29468693590831
2	48.12966225179965	17.168613122811596	19.95846110349927	14.743263521889476
3	28.562014745220853	16.925904177812576	39.68530479980862	14.826776277157958
4	24.099301885561427	21.265957678171418	38.429568734912245	16.205171701354907
5	20.52543441856419	24.228485678867358	38.91998869098106	16.326091211587396
6	21.727017681216154	27.214284160849044	38.503947282573236	12.554750875361561
7	64.99380178769492	4.968139013940541	25.798047019421066	4.240012178943477
8	66.56640786411778	3.64324394858746	25.49009373436854	4.300254452926209
9	61.29488266892847	5.996389813183707	27.53854853091494	5.17017898697288
10	32.890541745503576	21.18244492290294	33.389008503512315	12.538004828081165
11	24.704334398990888	21.12807463952502	39.089623975120155	15.077966986363933
12	22.34575150605685	19.28927165568387	41.85968117265827	16.505295665601007
13	20.837954807420456	18.66444835910485	43.778734694765234	16.71886213870947
14	17.842587155564253	20.46453970118092	44.092777451556074	17.600095691698744
15	17.460472803984253	20.91777038341924	44.79850372980144	16.823253082795066
16	21.2711772253757	20.81751158087036	42.431004110393424	15.480307083360517
17	21.028033318109653	20.637654683456212	41.3531676127096	16.98114438572454
18	21.47191231160697	20.36254104956395	41.71201148300385	16.453535155825232
19	20.43887692742655	22.123268306474415	41.050433874861355	16.387420891237685
20	20.709640938648572	22.13066266501381	40.605032513429464	16.55466388290816
21	20.514560361888606	22.091516060981707	41.87033774820034	15.52358582892934
22	19.89430416911333	19.861899480220092	42.5293055827407	17.714490767925884
23	18.65444422696331	21.901437550292513	42.16850438224484	17.27561384049934
24	18.438702942519736	21.927535286313912	42.07607490050238	17.557686870663968
25	19.96977012244188	21.432113264174333	40.81729409973685	17.78082251364694
26	18.339531545638415	22.09673560818599	40.87427415671691	18.68945868945869
27	18.620082207868467	21.359257084447925	42.03518844740219	17.98547226028142
28	17.571605663208718	22.889889302103043	41.29662251799656	18.241882516691675
29	18.713816576411997	22.807246471368607	40.40799460646789	18.070942345751504
30	19.6700811204628	21.981253126291296	40.86796720384507	17.480698549400838
31	20.041973858767754	22.537787346947653	39.73271568691416	17.68752310737044
32	20.184206520084384	23.43206976794763	38.273634762184386	18.110088949783606
33	20.208346925904177	23.542550183771557	37.30649616145799	18.94260672886627
34	19.226637089232508	24.020791196363717	38.349970640046976	18.4026010743568
35	19.496748657054	25.31980600682891	35.78043104760662	19.403014288510473
36	21.13960113960114	25.708662273547773	34.689980644179116	18.461755942671974
37	21.07979382788543	26.151236380244015	33.459254909636584	19.309714882233965
38	21.398403688480023	25.65385702790283	34.18890411256824	18.75883517104891
39	21.624801548465673	23.909658337139252	33.78177943063439	20.68376068376068
40	22.61173093234162	23.693264609295145	34.96900893847459	18.72599551988865
41	21.21680694199778	24.0149192057589	34.5312194167156	20.237054435527718
42	21.18157499836889	25.13994910941476	33.97185794132359	19.70661795089276
43	20.845407709292235	24.32722199641888	34.86142387925258	19.965946415036306
44	21.2296639680116	24.356661869871367	34.27789922335856	20.135774938758473
45	20.690079421748155	24.089945404413786	34.14124548240748	21.07872969143058
46	20.464227258247007	24.136082519525885	34.334581964782004	21.065108257445104
47	19.95474168708051	24.80990410071073	35.089912449331564	20.145441762877198
48	19.83280788285567	25.20756209286742	35.101556578383665	19.858073445893247
49	20.353058780881657	24.437291971043468	34.64260213329232	20.567047114782554
50	18.867883075368272	24.293827513099643	35.936858062460864	20.901431349071217
51	19.664077861874265	23.708325552272253	35.077169817538696	21.550426768314786
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	283.0
1	720.0
2	1157.0
3	16100.0
4	31256.0
5	20286.5
6	8944.5
7	8421.5
8	8032.0
9	7796.5
10	7587.0
11	7324.0
12	7061.0
13	6747.5
14	6434.0
15	6031.0
16	5628.0
17	5235.5
18	4836.0
19	4530.5
20	4229.0
21	3896.0
22	3566.0
23	3689.5
24	3813.0
25	3846.5
26	4282.5
27	4691.0
28	5334.0
29	5971.0
30	6641.5
31	7312.0
32	7991.5
33	8671.0
34	9756.5
35	10861.5
36	11667.0
37	12460.5
38	13580.5
39	14717.0
40	15659.5
41	16578.0
42	17507.5
43	18437.0
44	20353.0
45	22269.0
46	24954.0
47	27639.0
48	30166.5
49	32717.0
50	32904.0
51	33028.0
52	30441.0
53	27894.0
54	25777.0
55	23660.0
56	22008.5
57	20357.0
58	19186.5
59	18016.0
60	16559.5
61	15075.0
62	13804.0
63	12543.5
64	11196.0
65	9847.5
66	8518.5
67	7208.0
68	6019.5
69	4831.0
70	4111.5
71	3392.0
72	2900.0
73	2391.5
74	2018.5
75	1243.5
76	825.0
77	641.5
78	458.0
79	349.0
80	237.5
81	167.0
82	98.5
83	69.0
84	40.0
85	28.5
86	17.0
87	13.0
88	9.0
89	8.5
90	8.0
91	5.0
92	2.0
93	1.5
94	1.0
95	1.5
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
42	4645.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	455165.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.55429623069894
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.64433941852076	33.25726010397611
2	8.399483583752918	7.484661595508404
3	4.055928742880145	5.421271521026649
4	2.694063165753311	4.801283534047504
5	2.054958535178782	4.57786156590793
6	1.6299779628995517	4.35735126051227
7	1.292074229192728	4.029722057165336
8	1.00337115512576	3.5763596539849347
9	0.8280611273992778	3.3204312690546556
>10	3.344437392306723	21.242334650076028
>50	0.032081524577395396	0.945802968127388
>100	0.019248914746399275	1.9256909072026565
>500	0.0014806857497230211	0.4534400651652252
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.935619165743404E-4	4.606528848244975
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20948	4.555794784802418	No Hit
CGCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTG	756	0.1644157369348209	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	726	0.15789130292947087	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTT	580	0.12613905743676737	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCC	485	0.10547834975315892	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.3496226702333573E-4	0.0	0.0	0.06437441551945369	0.0
2	4.3496226702333573E-4	0.0	0.0	0.2520606337400231	0.0
3	4.3496226702333573E-4	0.0	0.0	0.5315238903025162	0.0
4	4.3496226702333573E-4	0.0	0.0	0.731389051999739	0.0
5	4.3496226702333573E-4	0.0	0.0	1.1789652247667515	0.0
6	4.3496226702333573E-4	0.0	0.0	1.8501120027837585	0.0
7	4.3496226702333573E-4	0.0	0.0	2.3894652138926946	0.0
8	4.3496226702333573E-4	0.0	0.0	3.1880559361475393	0.0
9	4.3496226702333573E-4	0.0	0.0	3.7304538831256386	0.0
10	4.3496226702333573E-4	0.0	0.0	4.427480916030534	0.0
11	4.3496226702333573E-4	0.0	0.0	5.118418477197103	0.0
12	4.3496226702333573E-4	0.0	0.0	5.609273395532938	0.0
13	4.3496226702333573E-4	0.0	0.0	5.891128944564059	0.0
14	4.3496226702333573E-4	0.0	0.0	6.079902568452186	0.0
15	4.3496226702333573E-4	0.0	0.0	6.2301820317087495	0.0
16	4.3496226702333573E-4	0.0	0.0	6.44026880668102	0.0
17	4.3496226702333573E-4	0.0	0.0	6.668189034601248	0.0
18	4.3496226702333573E-4	0.0	0.0	6.944390074161067	0.0
19	4.3496226702333573E-4	0.0	0.0	7.115112763967725	0.0
20	4.3496226702333573E-4	0.0	0.0	7.290837519845153	0.0
21	4.3496226702333573E-4	0.0	0.0	7.477001370131141	0.0
22	4.3496226702333573E-4	0.0	0.0	7.676649050694852	0.0
23	4.3496226702333573E-4	0.0	0.0	7.894782627607055	0.0
24	4.3496226702333573E-4	0.0	0.0	8.072899675953112	0.0
25	6.524434005350035E-4	0.0	0.0	8.240577629890607	0.0
26	6.524434005350035E-4	0.0	0.0	8.408473064961614	0.0
27	6.524434005350035E-4	0.0	0.0	8.599856462451882	0.0
28	6.524434005350035E-4	0.0	0.0	8.783193058002219	0.0
29	6.524434005350035E-4	0.0	0.0	8.977186229094626	0.0
30	6.524434005350035E-4	0.0	0.0	9.249037645984211	0.0
31	6.524434005350035E-4	0.0	0.0	9.449555251081968	0.0
32	6.524434005350035E-4	0.0	0.0	9.679432809203801	0.0
33	6.524434005350035E-4	0.0	0.0	9.897783867249515	0.0
34	6.524434005350035E-4	0.0	0.0	10.09656162327918	0.0
35	6.524434005350035E-4	0.0	0.0	10.315782605858942	0.0
36	6.524434005350035E-4	0.0	0.0	10.525869380831212	0.0
37	6.524434005350035E-4	0.0	0.0	10.748787542680672	0.0
38	6.524434005350035E-4	0.0	0.0	11.012157195363303	0.0
39	6.524434005350035E-4	0.0	0.0	11.450816641656337	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGA	30	2.193001E-6	44.908985	24
CGTTAGG	40	6.9230737E-9	44.908985	2
AAGCACG	20	7.0993195E-4	44.908985	1
TGCGACG	30	2.193001E-6	44.908985	1
GCTTACG	20	7.0993195E-4	44.908985	1
GCGATAC	20	7.0993195E-4	44.908985	9
CGGTCTA	30	2.193001E-6	44.908985	31
CGTTTTT	8805	0.0	44.14392	1
CCGCCCA	45	1.9586878E-8	39.919094	36
TCGGCGT	110	0.0	38.78503	4
GTCGGCG	110	0.0	38.78503	3
GCGATCA	35	6.3274438E-6	38.493412	9
ACGTAGG	70	0.0	38.493412	2
GTTTTTT	10370	0.0	38.477943	2
TCGCAAC	30	1.15250994E-4	37.424152	16
ATAGCGG	60	1.5825208E-10	37.424152	2
TAACGCC	30	1.15250994E-4	37.424152	12
TATAGCG	30	1.15250994E-4	37.424152	1
ACAACGA	85	0.0	36.983868	13
CGTAGGG	165	0.0	36.743713	3
>>END_MODULE