FastQCFastQC Report
Sat 18 Jun 2016
SRR3552053_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552053_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106498
Sequences flagged as poor quality0
Sequence length42-51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36363.4141486225093427No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGC24202.272343142594227No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTC23192.177505680857857Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCG21552.0235121786324624No Hit
GCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC9620.9033033484196886Illumina Single End Adapter 1 (95% over 24bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAAGAACT8630.8103438562226521No Hit
GAACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCT7920.7436759375762926Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTA5330.5004788822325302No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCT4270.4009464966478244No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTC3290.30892598922045483No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTT1890.1774681214670698No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCAAGAAC1490.13990873068038837No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATG1450.13615279160172022No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCAAGAACTCGT1360.12770192867471689No Hit
TAGAATGGGACCACCCATTGGAAAAATGGAAAAAAAATAAATAAAAAAATA1330.12488497436571577No Hit
CTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGCT1310.12300700482638172Illumina Single End Adapter 1 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC1220.11455614189937839Illumina Single End Adapter 1 (95% over 24bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC1080.1014103551240399No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCACT302.0432362E-645.32417338
ACCACTC502.0008883E-1145.32417339
CCGCAAA253.7062E-545.32417341
CTGTAGT351.13221176E-745.3241742
CACTCGA406.3009793E-945.3241741
TGTAGTT351.13221176E-745.3241743
CTCGAAT750.045.3241743
CCGCCAA206.760805E-445.3241741
TCGAATG700.045.3241744
GTAGTTT351.13221176E-745.3241744
CCCGCCA351.13221176E-745.3241740
TATTTGG207.066362E-444.9196816
ACAACGA207.066362E-444.9196813
ACAACCA207.066362E-444.9196836
ATGGGCT453.8380676E-1044.919685
GGGTGTA207.066362E-444.919687
GACAACC253.9079285E-544.9196835
CGACCTG302.1734904E-644.9196812
AAGACGG207.066362E-444.919682
GTAGGGC302.1734904E-644.919684
Produced by FastQC (version 0.11.5)