##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552053_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	106498
Sequences flagged as poor quality	0
Sequence length	42-51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.103955003849837	30.0	28.0	31.0	25.0	33.0
2	28.158754155007607	31.0	26.0	33.0	16.0	33.0
3	27.867077316005933	31.0	25.0	33.0	16.0	34.0
4	32.90564142049616	35.0	32.0	35.0	28.0	37.0
5	34.1336738718098	35.0	33.0	35.0	32.0	37.0
6	34.18649176510357	35.0	35.0	37.0	31.0	37.0
7	34.715637852354035	35.0	35.0	37.0	32.0	37.0
8	33.43813968337434	35.0	33.0	37.0	28.0	37.0
9	35.477642772634226	38.0	34.0	39.0	28.0	39.0
10	35.62087550939924	37.0	34.0	39.0	31.0	39.0
11	36.20138406355049	37.0	35.0	39.0	32.0	39.0
12	36.15430336719938	37.0	35.0	39.0	32.0	39.0
13	36.040676820221975	37.0	35.0	39.0	31.0	39.0
14	36.84523653026348	39.0	36.0	40.0	31.0	41.0
15	37.102180322635164	39.0	36.0	40.0	31.0	41.0
16	36.991830832503894	38.0	35.0	40.0	32.0	41.0
17	36.84536798813123	38.0	35.0	40.0	31.0	41.0
18	36.57047080696351	38.0	35.0	39.0	31.0	41.0
19	36.0002816954309	37.0	35.0	39.0	30.0	41.0
20	35.457370091457115	36.0	34.0	39.0	30.0	41.0
21	35.87493661852805	37.0	35.0	40.0	30.0	41.0
22	36.23851152134312	37.0	35.0	40.0	31.0	41.0
23	36.23432364927041	37.0	35.0	40.0	31.0	41.0
24	36.27146049691074	38.0	35.0	40.0	31.0	41.0
25	35.88365039719056	37.0	35.0	40.0	30.0	41.0
26	35.75142256192605	37.0	35.0	40.0	30.0	41.0
27	35.712003981295425	37.0	35.0	40.0	30.0	41.0
28	35.587381922665216	37.0	35.0	40.0	30.0	41.0
29	35.56532517042574	37.0	35.0	40.0	29.0	41.0
30	35.206445191459	37.0	34.0	40.0	27.0	41.0
31	35.0078968619129	37.0	34.0	40.0	25.0	41.0
32	34.310456534395016	37.0	34.0	40.0	22.0	41.0
33	33.891256173824864	37.0	33.0	40.0	18.0	41.0
34	33.24988262690379	37.0	33.0	40.0	14.0	41.0
35	32.892420514939246	37.0	33.0	40.0	12.0	41.0
36	32.89950046010254	37.0	33.0	40.0	11.0	41.0
37	32.74161956093072	37.0	33.0	40.0	10.0	41.0
38	32.550094837461735	37.0	32.0	40.0	10.0	41.0
39	32.50483577156378	37.0	32.0	40.0	10.0	41.0
40	32.329114161768295	36.0	31.0	40.0	10.0	41.0
41	32.12651880786493	36.0	30.0	40.0	10.0	41.0
42	32.11692238351894	37.0	31.0	40.0	8.0	41.0
43	32.17554235023172	37.0	31.0	40.0	9.0	41.0
44	32.306929004008985	37.0	31.0	40.0	10.0	41.0
45	32.24722072161724	36.0	31.0	40.0	10.0	41.0
46	32.15289111294343	36.0	31.0	40.0	10.0	41.0
47	31.967814392539307	35.0	31.0	40.0	10.0	41.0
48	32.03735084776284	35.0	31.0	40.0	10.0	41.0
49	32.15216134504753	36.0	31.0	40.0	10.0	41.0
50	31.840067100736402	35.0	31.0	40.0	8.0	41.0
51	31.199672078322102	35.0	29.0	39.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	1.0
14	6.0
15	9.0
16	18.0
17	52.0
18	142.0
19	370.0
20	665.0
21	1010.0
22	1212.0
23	1592.0
24	1909.0
25	2682.0
26	3343.0
27	3624.0
28	3323.0
29	2998.0
30	2972.0
31	3466.0
32	4306.0
33	6053.0
34	8621.0
35	9423.0
36	10170.0
37	14021.0
38	17465.0
39	7042.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.602734323649265	12.984281394955774	14.447219666097016	26.96576461529794
2	37.377227741366035	31.532047550188736	17.199384025991098	13.891340682454132
3	22.970384420364702	30.177092527559203	34.30299160547616	12.549531446599937
4	20.479257826438054	16.635993164190875	48.41781066311104	14.466938346260024
5	29.85314278202408	20.05014178670022	33.84195008356964	16.25476534770606
6	17.792822400420665	36.198801855433906	34.511446224342244	11.496929519803189
7	54.526845574564774	4.623561005840485	36.48237525587335	4.367218163721384
8	53.4779995868467	15.89982910477192	24.16852898645984	6.453642321921538
9	49.5671280211835	5.4104302428214615	25.484985633533025	19.537456102462016
10	38.0561137298353	18.146819658585137	30.65503577531972	13.142030836259835
11	33.08512835921801	16.979661589889012	34.44102236661721	15.494187684275762
12	22.19102706154106	14.879152660143852	38.66927078442788	24.26054949388721
13	24.26242746342654	19.695205543766082	42.60643392364176	13.435933069165618
14	16.17870758136303	29.982722680238126	36.235422261450914	17.603147476947925
15	13.16926139458018	18.698003718379688	46.79993990497474	21.33279498206539
16	14.681965858513774	18.73274615485737	39.36881443782982	27.216473548799037
17	15.406862100696728	24.014535484234443	39.97164265995606	20.606959755112772
18	16.29701966234108	17.994704125899077	40.135964994647786	25.57231121711206
19	16.950553062029332	20.298033765892317	37.0420101785949	25.709402993483444
20	23.35818513023719	19.276418336494583	41.6111100677947	15.75428646547353
21	18.074517831320776	27.442768878288792	37.111495051550264	17.371218238840168
22	16.16274484027869	17.747751131476647	44.039324682153655	22.050179346091006
23	21.175045540761328	22.788221375049297	37.37065484797837	18.66607823621101
24	18.904580367706437	23.868053860166388	37.30116997502301	19.92619579710417
25	15.531747075062444	29.42684369659524	36.287066423782605	18.75434280455971
26	16.39749103269545	19.22007925031456	43.78392082480423	20.59850889218577
27	22.97414035944337	20.80132960243385	36.74810794568912	19.47642209243366
28	14.809667787188491	25.27465304512761	42.304080827808974	17.611598339874927
29	19.643561381434395	18.147758643354805	42.063700726774215	20.14497924843659
30	16.41251478901012	27.201449792484368	37.42699393415839	18.959041484347125
31	19.52149336137768	22.431407162575823	33.513305414186185	24.533794061860316
32	25.05305263948619	27.14604969107401	32.09543841198896	15.705459257450844
33	24.47276005183196	21.46237488027944	30.62780521699938	23.43705985088922
34	22.033277620236998	25.257751319273602	31.437210088452368	21.271760972037036
35	20.8510957952262	24.451163402129616	30.95457191684351	23.74316888580067
36	22.318728990215778	27.085954665815322	30.341414862250932	20.253901481717968
37	17.221919660463108	25.786399744596146	34.74525343198933	22.246427162951417
38	20.82104828259686	27.48596217769348	28.28973314052846	23.403256399181206
39	20.62667843527578	29.330128265319537	30.636256079926383	19.4069372194783
40	21.235140566020018	25.44930421228568	30.56864917651036	22.746906045183948
41	16.23880260662172	26.566696088189452	29.236229788352837	27.958271516835996
42	19.10552310841518	26.143213957069616	32.16586226971398	22.585400664801217
43	22.31573360628548	22.513813463743805	28.735795589169104	26.43465734080161
44	20.184242699951664	23.181977576222835	33.03668742240293	23.597092301422574
45	18.322860690151924	25.16182840029191	31.516495597698864	24.998815311857307
46	25.365594760835158	26.880100082454295	29.37837043776596	18.375934718944585
47	17.801597907366865	24.979860301574213	37.53092036052429	19.687621430534627
48	19.972894335295177	25.904864803389156	31.27481921658942	22.847421644726243
49	19.631704150199504	21.689270516429254	37.548927620293234	21.13009771307801
50	20.394643314094	22.476851193691772	33.44232464246112	23.68618084975311
51	20.109370409333447	22.143243012709334	31.279557969160198	26.467828608797024
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	53.0
1	138.0
2	223.0
3	2914.5
4	5644.5
5	3773.5
6	1833.0
7	1803.0
8	1804.0
9	1765.0
10	1726.0
11	1670.5
12	1615.0
13	1478.0
14	1341.0
15	1244.0
16	1147.0
17	1054.5
18	964.0
19	865.5
20	760.0
21	734.0
22	713.0
23	660.5
24	608.0
25	662.5
26	682.5
27	649.5
28	809.0
29	967.0
30	1021.5
31	1076.0
32	1353.0
33	1630.0
34	1706.5
35	1790.5
36	1878.5
37	1955.0
38	2078.0
39	2206.5
40	2666.5
41	3125.0
42	3594.5
43	4064.0
44	4629.0
45	5194.0
46	10255.0
47	15316.0
48	11885.5
49	8460.0
50	8528.5
51	8575.5
52	7506.0
53	6453.0
54	6233.0
55	6013.0
56	5323.5
57	4634.0
58	4281.0
59	3928.0
60	3442.5
61	2955.0
62	2646.0
63	2337.0
64	2050.0
65	1760.5
66	1455.5
67	1155.0
68	1045.0
69	935.0
70	848.0
71	761.0
72	682.0
73	599.5
74	490.0
75	318.5
76	253.0
77	199.5
78	146.0
79	107.5
80	68.5
81	64.0
82	60.0
83	47.0
84	34.0
85	20.5
86	7.0
87	5.0
88	3.0
89	2.5
90	2.0
91	1.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
42	985.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	105513.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.58485605363481
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.16317297541849	34.06730642828973
2	7.540340823405218	6.572893387669251
3	3.2681991511730617	4.2733196867546805
4	2.216860202081134	3.8648613119495203
5	1.6524118318719434	3.601006591673083
6	1.316328069457311	3.442318165599354
7	1.1116616756791693	3.391612988037334
8	0.833746256759377	2.9070968468891434
9	0.7217183359544994	2.831039080546114
>10	3.0936941206885407	19.150594377359198
>50	0.04093327875562833	1.198144566095138
>100	0.023698214016416398	1.854494920092396
>500	0.008617532369605963	2.9578020244511634
>1k	0.008617532369605963	9.88750962459389
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3636	3.4141486225093427	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGC	2420	2.272343142594227	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTC	2319	2.177505680857857	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCG	2155	2.0235121786324624	No Hit
GCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC	962	0.9033033484196886	Illumina Single End Adapter 1 (95% over 24bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAAGAACT	863	0.8103438562226521	No Hit
GAACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCT	792	0.7436759375762926	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTA	533	0.5004788822325302	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCT	427	0.4009464966478244	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTC	329	0.30892598922045483	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTT	189	0.1774681214670698	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGCAAGAAC	149	0.13990873068038837	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATG	145	0.13615279160172022	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCAAGAACTCGT	136	0.12770192867471689	No Hit
TAGAATGGGACCACCCATTGGAAAAATGGAAAAAAAATAAATAAAAAAATA	133	0.12488497436571577	No Hit
CTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGCT	131	0.12300700482638172	Illumina Single End Adapter 1 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC	122	0.11455614189937839	Illumina Single End Adapter 1 (95% over 24bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	108	0.1014103551240399	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12864091344438394	0.0
2	0.0	0.0	0.0	1.3361753272361923	0.0
3	0.0	0.0	0.0	1.515521418242596	0.0
4	0.0	0.0	0.0	2.506150350241319	0.0
5	0.0	0.0	0.0	5.345640293714436	0.0
6	0.0	0.0	0.0	5.777573287761273	0.0
7	0.0	0.0	0.0	6.444252474224868	0.0
8	0.0	0.0	0.0	7.179477548874157	0.0
9	0.0	0.0	0.0	7.3400439444872205	0.0
10	0.0	0.0	0.0	9.902533380908562	0.0
11	0.0	0.0	0.0	10.464985257939116	0.0
12	0.0	0.0	0.0	13.519502713665984	0.0
13	0.0	0.0	0.0	13.947679768634153	0.0
14	0.0	0.0	0.0	14.122330935792222	0.0
15	0.0	0.0	0.0	14.835020375969501	0.0
16	0.0	0.0	0.0	15.290427989258013	0.0
17	0.0	0.0	0.0	15.696069409754173	0.0
18	0.0	0.0	0.0	16.107344738868335	0.0
19	0.0	0.0	0.0	17.22755356908111	0.0
20	0.0	0.0	0.0	17.62004920280193	0.0
21	0.0	0.0	0.0	17.906439557550378	0.0
22	0.0	0.0	0.0	18.2247553944675	0.0
23	0.0	0.0	0.0	18.50363387105861	0.0
24	0.0	0.0	0.0	18.725234276700032	0.0
25	0.0	0.0	0.0	18.940261788953784	0.0
26	0.0	0.0	0.0	19.150594377359198	0.0
27	0.0	0.0	0.0	19.42289996056264	0.0
28	0.0	0.0	0.0	19.6548291986704	0.0
29	0.0	0.0	0.0	19.86703975661515	0.0
30	0.0	0.0	0.0	20.10741985764991	0.0
31	0.0	0.0	0.0	20.33183721760033	0.0
32	0.0	0.0	0.0	20.559071531859754	0.0
33	0.0	0.0	0.0	20.78724483088884	0.0
34	0.0	0.0	0.0	20.99476046498526	0.0
35	0.0	0.0	0.0	21.24453041371669	0.0
36	0.0	0.0	0.0	21.45016807827377	0.0
37	0.0	0.0	0.0	21.67176848391519	0.0
38	0.0	0.0	0.0	21.88491802662961	0.0
39	0.0	0.0	0.0	22.132810005821707	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCACT	30	2.0432362E-6	45.324173	38
ACCACTC	50	2.0008883E-11	45.324173	39
CCGCAAA	25	3.7062E-5	45.324173	41
CTGTAGT	35	1.13221176E-7	45.32417	42
CACTCGA	40	6.3009793E-9	45.32417	41
TGTAGTT	35	1.13221176E-7	45.32417	43
CTCGAAT	75	0.0	45.32417	43
CCGCCAA	20	6.760805E-4	45.32417	41
TCGAATG	70	0.0	45.32417	44
GTAGTTT	35	1.13221176E-7	45.32417	44
CCCGCCA	35	1.13221176E-7	45.32417	40
TATTTGG	20	7.066362E-4	44.91968	16
ACAACGA	20	7.066362E-4	44.91968	13
ACAACCA	20	7.066362E-4	44.91968	36
ATGGGCT	45	3.8380676E-10	44.91968	5
GGGTGTA	20	7.066362E-4	44.91968	7
GACAACC	25	3.9079285E-5	44.91968	35
CGACCTG	30	2.1734904E-6	44.91968	12
AAGACGG	20	7.066362E-4	44.91968	2
GTAGGGC	30	2.1734904E-6	44.91968	4
>>END_MODULE