FastQCFastQC Report
Sat 18 Jun 2016
SRR3552051_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552051_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124057
Sequences flagged as poor quality0
Sequence length42-51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33462.697147279073329No Hit
GAATCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTC12491.0067952634675996No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGC12230.9858371555011004No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCG10940.8818526967442385No Hit
GCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC5610.4522114834310035RNA PCR Primer, Index 40 (95% over 24bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAAGAAGCT4990.4022344567416591No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTA3260.26278243065687545No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTC2150.17330743126143627No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCT2140.17250135018580168No Hit
GAACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCT2020.16282837727818664RNA PCR Primer, Index 40 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGCT1310.10559662090813093Illumina PCR Primer Index 8 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCAG206.7558594E-445.3368638
CGCTCGA351.1317752E-745.3368641
CCGCGAA206.7558594E-445.3368642
CCCGCGA206.7558594E-445.3368641
CTCGAAT551.8189894E-1245.33685743
TCGAATG600.045.33685744
CGGCGAG253.9132974E-544.9165731
ATAGCGG253.9132974E-544.916572
GTGGCGT253.9132974E-544.9165734
GGTATGC253.9132974E-544.916578
CGATGAA3750.044.9165719
CGGTCGG253.9132974E-544.916571
GGCGAAC502.1827873E-1144.9165732
TGGCGTT207.07332E-444.91656535
TAACGGG207.07332E-444.9165653
TAGCCGG207.07332E-444.9165652
AACGGGA302.1774613E-644.9165654
ACGGCGC207.07332E-444.91656530
TCTGGGT302.1774613E-644.9165654
GGTAGAC207.07332E-444.9165658
Produced by FastQC (version 0.11.5)