##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552051_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	124057
Sequences flagged as poor quality	0
Sequence length	42-51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.92585666266313	30.0	28.0	33.0	25.0	33.0
2	28.156524823266725	31.0	26.0	33.0	16.0	33.0
3	27.850020555067427	31.0	25.0	33.0	16.0	34.0
4	32.86303070362817	35.0	32.0	35.0	28.0	37.0
5	34.07987457378463	35.0	33.0	35.0	32.0	37.0
6	34.01448527692915	35.0	35.0	37.0	30.0	37.0
7	34.57926598257253	35.0	35.0	37.0	32.0	37.0
8	33.794134954093686	35.0	35.0	37.0	28.0	37.0
9	35.80153477836801	39.0	35.0	39.0	30.0	39.0
10	35.656964137452945	37.0	34.0	39.0	31.0	39.0
11	36.21330517423443	37.0	35.0	39.0	32.0	39.0
12	36.30462609929307	37.0	35.0	39.0	32.0	39.0
13	36.310123572228896	38.0	35.0	39.0	32.0	39.0
14	37.145739458474736	39.0	36.0	40.0	32.0	41.0
15	37.442151591607086	39.0	36.0	40.0	32.0	41.0
16	37.1823436001193	39.0	36.0	40.0	32.0	41.0
17	37.07947153324682	39.0	36.0	40.0	32.0	41.0
18	36.887237318329475	39.0	36.0	40.0	32.0	41.0
19	36.34040803824048	37.0	35.0	40.0	31.0	41.0
20	36.13529264773451	38.0	35.0	40.0	31.0	41.0
21	36.41884778771049	38.0	35.0	40.0	31.0	41.0
22	36.63802123217553	38.0	35.0	40.0	31.0	41.0
23	36.77775538663679	38.0	35.0	40.0	31.0	41.0
24	36.748204454404025	38.0	35.0	40.0	32.0	41.0
25	36.17927243122113	38.0	35.0	40.0	31.0	41.0
26	36.201262322964446	38.0	35.0	40.0	30.0	41.0
27	36.258768146900216	38.0	35.0	40.0	31.0	41.0
28	36.21747261339546	38.0	35.0	40.0	30.0	41.0
29	36.23036991060561	38.0	35.0	40.0	30.0	41.0
30	35.77073442046801	38.0	35.0	40.0	29.0	41.0
31	35.696671691238706	38.0	35.0	40.0	29.0	41.0
32	35.20002095810796	38.0	35.0	40.0	25.0	41.0
33	34.85399453477031	38.0	34.0	40.0	22.0	41.0
34	34.53111875992487	38.0	34.0	40.0	19.0	41.0
35	34.18173097850182	38.0	34.0	40.0	16.0	41.0
36	34.019491040408845	38.0	34.0	40.0	15.0	41.0
37	33.84853736588826	38.0	34.0	40.0	13.0	41.0
38	33.72507798834407	38.0	33.0	40.0	12.0	41.0
39	33.67236028599756	38.0	33.0	40.0	12.0	41.0
40	33.555663928677944	38.0	33.0	40.0	13.0	41.0
41	33.4506799293873	38.0	33.0	40.0	13.0	41.0
42	33.43255922680703	38.0	33.0	40.0	11.0	41.0
43	33.355871538736864	38.0	33.0	40.0	11.0	41.0
44	33.42830182539941	38.0	33.0	40.0	11.0	41.0
45	33.40606188787297	38.0	33.0	40.0	12.0	41.0
46	33.203055347671885	37.0	33.0	40.0	11.0	41.0
47	33.17091562815215	37.0	33.0	40.0	12.0	41.0
48	33.17664237139231	37.0	33.0	40.0	12.0	41.0
49	33.195376305599844	37.0	33.0	40.0	12.0	41.0
50	32.994972830507926	37.0	33.0	40.0	11.0	41.0
51	32.13556275013829	35.0	31.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	3.0
12	5.0
13	9.0
14	14.0
15	11.0
16	40.0
17	69.0
18	187.0
19	469.0
20	691.0
21	794.0
22	994.0
23	1341.0
24	1751.0
25	2460.0
26	3268.0
27	3606.0
28	3291.0
29	3086.0
30	3049.0
31	3559.0
32	4545.0
33	6376.0
34	8978.0
35	10395.0
36	12420.0
37	18240.0
38	24369.0
39	10031.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.20640512022699	14.815770170163715	17.292051234513167	25.685773475096124
2	38.637884198392676	26.19279847167028	19.750598515198657	15.418718814738385
3	27.88476264942728	24.511313347896532	33.271802477893225	14.332121524782963
4	24.51453767219907	19.974689054225074	39.77848892041562	15.732284353160242
5	27.358391707037892	23.469856598176644	31.75798221785147	17.41376947693399
6	20.443828240244404	34.808999089128385	32.30692343035862	12.440249240268585
7	62.34714687603279	5.7401033395938965	27.67034508330848	4.2424047010648325
8	63.05327389828869	10.986078979823791	20.723538373489607	5.2371087483979135
9	57.65656109691513	7.1854067082067115	22.184157282539477	12.973874912338685
10	36.84354772403008	21.53606809772927	28.662630887414657	12.957753290825991
11	30.793103170316872	20.931507291003328	32.55680856380535	15.718580974874452
12	23.37070862587359	19.495876895298128	36.41068218641431	20.72273229241397
13	24.469397131963532	20.19877959325149	38.84101662945259	16.490806645332388
14	18.10780528305537	27.75659575840138	35.56590921914926	18.569689739393986
15	17.780536366347725	21.293437693963256	40.66517810361366	20.260847836075353
16	18.93565054773209	21.514303908687136	36.97171461505599	22.578330928524792
17	19.314508653280345	24.857928210419402	36.72666596806307	19.100897168237182
18	19.872316757619483	21.69083566425111	36.974132858282886	21.462714719846524
19	20.127038377520012	22.434042415986198	35.36519503131625	22.07372417517754
20	24.409747132366576	21.605391070233846	38.14053217472613	15.844329622673447
21	21.125772830231266	26.8481423861612	35.53769638150205	16.488388402105482
22	19.389474193314364	20.966168777255618	39.930032162634916	19.714324866795103
23	22.645235657802463	23.25302078883094	36.508217996566096	17.5935255568005
24	19.5837397325423	24.401686321610228	36.54932813142346	19.465245814424016
25	18.880030953513305	27.044826168616037	35.38454097713148	18.690601900739175
26	18.398800551359457	22.84836808886238	39.26340311308512	19.489428246693052
27	22.485631604826814	23.836623487590384	35.52318692214063	18.154557985442178
28	17.43150326059795	27.60021602972827	37.320747720805755	17.64753298886802
29	21.39419782841758	21.718242420822687	38.60161054998912	18.285949200770613
30	18.55276203680566	28.756942373263904	35.25718016718121	17.43311542274922
31	21.380494450131796	24.137291728802083	33.2758328832714	21.206380937794723
32	25.54712753008698	25.18036064067324	32.480230861620065	16.792280967619725
33	22.06888768872373	25.726077528877855	31.73702410988497	20.468010672513444
34	21.606197151309477	25.694640366928105	31.684628839968727	21.014533641793694
35	23.710874839791384	25.487477530490015	30.91482141273769	19.886826216980904
36	21.947169446302908	29.496924800696455	29.277670748123846	19.27823500487679
37	21.36598499077037	27.97343156774708	31.43635586867327	19.224227572809273
38	20.626808644413455	28.835938318676092	28.973778182609607	21.563474854300846
39	22.921721466745126	27.668732921157208	29.740361285538096	19.669184326559567
40	22.129343769396325	26.68853833318555	30.828570737644796	20.35354715977333
41	19.395116760843806	27.547014678736385	29.605745745907125	23.452122814512684
42	22.342149173363858	25.54954577331388	30.732647089644278	21.375657963677988
43	22.381479191748284	25.470992093189732	29.696905606351482	22.450623108710506
44	20.9522337552468	26.70988839358344	31.22864673152637	21.10923111964338
45	20.66020238831224	25.50841115413399	30.634009045651254	23.197377411902515
46	23.447922428659748	27.135326847362766	29.347118732307294	20.069631991670192
47	19.4766212214883	27.58191520515407	33.87970585364267	19.061757719714965
48	20.317736634887577	26.93114892786256	31.21156411674747	21.53955032050239
49	21.763251228321348	23.840822568574495	34.13675853317281	20.259167669931344
50	20.32180392412065	25.125272508378615	32.707513096671335	21.845410470829403
51	20.46985325220447	24.878794780854456	31.018774607099857	23.632577359841214
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	56.0
1	198.5
2	341.0
3	2836.5
4	5369.0
5	3641.0
6	1849.0
7	1757.0
8	1690.0
9	1682.0
10	1676.0
11	1592.0
12	1508.0
13	1459.5
14	1411.0
15	1325.5
16	1240.0
17	1128.5
18	1016.5
19	933.5
20	850.0
21	862.0
22	875.0
23	849.0
24	823.0
25	862.5
26	981.5
27	1061.5
28	1181.0
29	1300.0
30	1586.5
31	1873.0
32	2058.0
33	2243.0
34	2477.5
35	2717.0
36	2999.0
37	3284.5
38	3614.5
39	3943.0
40	4461.0
41	4972.0
42	5244.5
43	5517.0
44	6085.0
45	6653.0
46	9501.5
47	12350.0
48	10781.5
49	9221.5
50	9157.0
51	9077.0
52	8470.0
53	7870.0
54	7361.0
55	6852.0
56	6260.0
57	5668.0
58	5329.5
59	4991.0
60	4565.0
61	4134.0
62	3770.5
63	3403.0
64	2948.0
65	2496.5
66	2024.5
67	1558.0
68	1300.0
69	1042.0
70	955.5
71	869.0
72	731.0
73	594.5
74	499.5
75	334.5
76	266.0
77	206.0
78	146.0
79	106.0
80	65.5
81	53.5
82	42.0
83	29.0
84	16.0
85	13.0
86	10.0
87	6.5
88	3.0
89	2.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
42	1125.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	122932.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.302796295251376
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.2753199939923	35.87705651434421
2	9.019908883066602	8.71373642760989
3	4.549171436677069	6.592131036539656
4	3.082288937469753	5.955326986788331
5	2.35468851693006	5.686901988602014
6	1.813994626437261	5.2572607752887786
7	1.3033392854162842	4.406845240494288
8	0.9478831166663886	3.6628324076835646
9	0.6808737880279693	2.9599297097302046
>10	1.9291423994125794	12.759457346219882
>50	0.023363316255861688	0.7311155356005707
>100	0.011681658127930844	1.3735621528813369
>500	0.0016688083039901205	0.4522114834310035
>1k	0.006675233215960482	5.571632394786268
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3346	2.697147279073329	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTC	1249	1.0067952634675996	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGC	1223	0.9858371555011004	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCG	1094	0.8818526967442385	No Hit
GCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	561	0.4522114834310035	RNA PCR Primer, Index 40 (95% over 24bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAAGAAGCT	499	0.4022344567416591	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTA	326	0.26278243065687545	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTC	215	0.17330743126143627	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCT	214	0.17250135018580168	No Hit
GAACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCT	202	0.16282837727818664	RNA PCR Primer, Index 40 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGCT	131	0.10559662090813093	Illumina PCR Primer Index 8 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.060810756345873E-4	0.0	0.0	0.11526959381574599	0.0
2	8.060810756345873E-4	0.0	0.0	0.7738378326092038	0.0
3	8.060810756345873E-4	0.0	0.0	0.8826587778198731	0.0
4	8.060810756345873E-4	0.0	0.0	1.2204067485107652	0.0
5	8.060810756345873E-4	0.0	0.0	2.6407216037789083	0.0
6	8.060810756345873E-4	0.0	0.0	2.9365533585368016	0.0
7	8.060810756345873E-4	0.0	0.0	3.342012139580999	0.0
8	8.060810756345873E-4	0.0	0.0	3.84823105507952	0.0
9	8.060810756345873E-4	0.0	0.0	4.00299862160136	0.0
10	8.060810756345873E-4	0.0	0.0	5.295952666919239	0.0
11	8.060810756345873E-4	0.0	0.0	5.840057392972585	0.0
12	8.060810756345873E-4	0.0	0.0	7.36274454484632	0.0
13	8.060810756345873E-4	0.0	0.0	7.639230353788984	0.0
14	8.060810756345873E-4	0.0	0.0	7.75449994760473	0.0
15	8.060810756345873E-4	0.0	0.0	8.190589809523042	0.0
16	8.060810756345873E-4	0.0	0.0	8.52591953698703	0.0
17	8.060810756345873E-4	0.0	0.0	8.883013453493152	0.0
18	8.060810756345873E-4	0.0	0.0	9.236883045696736	0.0
19	8.060810756345873E-4	0.0	0.0	9.915603311381059	0.0
20	8.060810756345873E-4	0.0	0.0	10.190476958172454	0.0
21	8.060810756345873E-4	0.0	0.0	10.41859790257704	0.0
22	8.060810756345873E-4	0.0	0.0	10.723296549166916	0.0
23	8.060810756345873E-4	0.0	0.0	10.963508709706023	0.0
24	8.060810756345873E-4	0.0	0.0	11.16502897861467	0.0
25	8.060810756345873E-4	0.0	0.0	11.36735532859895	0.0
26	8.060810756345873E-4	0.0	0.0	11.55356005707054	0.0
27	8.060810756345873E-4	0.0	0.0	11.754274244903552	0.0
28	8.060810756345873E-4	0.0	0.0	11.972722216400525	0.0
29	8.060810756345873E-4	0.0	0.0	12.175854647460442	0.0
30	8.060810756345873E-4	0.0	0.0	12.401557348638127	0.0
31	8.060810756345873E-4	0.0	0.0	12.60065937431987	0.0
32	8.060810756345873E-4	0.0	0.0	12.819107345816843	0.0
33	8.060810756345873E-4	0.0	0.0	13.056901263129046	0.0
34	8.060810756345873E-4	0.0	0.0	13.28260396430673	0.0
35	8.060810756345873E-4	0.0	0.0	13.491378962896087	0.0
36	8.060810756345873E-4	0.0	0.0	13.704990447939254	0.0
37	8.060810756345873E-4	0.0	0.0	13.914571527604247	0.0
38	8.060810756345873E-4	0.0	0.0	14.135437742328124	0.0
39	8.060810756345873E-4	0.0	0.0	14.38612895685048	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCAG	20	6.7558594E-4	45.33686	38
CGCTCGA	35	1.1317752E-7	45.33686	41
CCGCGAA	20	6.7558594E-4	45.33686	42
CCCGCGA	20	6.7558594E-4	45.33686	41
CTCGAAT	55	1.8189894E-12	45.336857	43
TCGAATG	60	0.0	45.336857	44
CGGCGAG	25	3.9132974E-5	44.91657	31
ATAGCGG	25	3.9132974E-5	44.91657	2
GTGGCGT	25	3.9132974E-5	44.91657	34
GGTATGC	25	3.9132974E-5	44.91657	8
CGATGAA	375	0.0	44.91657	19
CGGTCGG	25	3.9132974E-5	44.91657	1
GGCGAAC	50	2.1827873E-11	44.91657	32
TGGCGTT	20	7.07332E-4	44.916565	35
TAACGGG	20	7.07332E-4	44.916565	3
TAGCCGG	20	7.07332E-4	44.916565	2
AACGGGA	30	2.1774613E-6	44.916565	4
ACGGCGC	20	7.07332E-4	44.916565	30
TCTGGGT	30	2.1774613E-6	44.916565	4
GGTAGAC	20	7.07332E-4	44.916565	8
>>END_MODULE