##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3552050_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 267334 Sequences flagged as poor quality 0 Sequence length 51 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.707837386939186 31.0 30.0 33.0 28.0 34.0 2 30.536157017064795 31.0 30.0 33.0 26.0 34.0 3 29.69820898202249 31.0 28.0 33.0 25.0 34.0 4 34.18904815698714 35.0 33.0 37.0 32.0 37.0 5 35.02364457943995 35.0 35.0 37.0 33.0 37.0 6 35.084747918334365 35.0 35.0 37.0 33.0 37.0 7 35.620800197505744 37.0 35.0 37.0 35.0 37.0 8 35.240998152124305 37.0 35.0 37.0 33.0 37.0 9 37.24186598038409 39.0 37.0 39.0 34.0 39.0 10 36.50632916127391 38.0 35.0 39.0 32.0 39.0 11 36.63905451607353 39.0 35.0 39.0 32.0 39.0 12 36.869994089790296 39.0 37.0 39.0 33.0 39.0 13 36.875814524153306 39.0 37.0 39.0 33.0 39.0 14 37.84738566736741 40.0 37.0 41.0 33.0 41.0 15 38.13416924147322 40.0 37.0 41.0 33.0 41.0 16 37.78477111029648 39.0 36.0 41.0 33.0 41.0 17 37.67255193877322 39.0 36.0 41.0 33.0 41.0 18 37.522907673546946 39.0 36.0 40.0 33.0 41.0 19 36.902283286076596 38.0 36.0 40.0 32.0 41.0 20 36.934321859546486 38.0 35.0 40.0 32.0 41.0 21 37.01576679359902 39.0 35.0 40.0 32.0 41.0 22 37.24791459372919 39.0 35.0 40.0 33.0 41.0 23 37.334207395991534 39.0 35.0 41.0 33.0 41.0 24 37.308082772860914 39.0 35.0 41.0 33.0 41.0 25 36.69423642335057 38.0 35.0 40.0 31.0 41.0 26 36.843689915985244 39.0 35.0 40.0 32.0 41.0 27 37.016713175278866 39.0 35.0 40.0 33.0 41.0 28 36.96481554908841 39.0 35.0 41.0 33.0 41.0 29 36.932099919950325 39.0 35.0 41.0 32.0 41.0 30 36.64482632212887 39.0 35.0 41.0 31.0 41.0 31 36.53676300059102 39.0 35.0 41.0 31.0 41.0 32 36.29987955142256 39.0 35.0 41.0 30.0 41.0 33 36.005367817037865 39.0 35.0 41.0 29.0 41.0 34 35.74582357649981 39.0 35.0 41.0 27.0 41.0 35 35.53081912513934 39.0 35.0 41.0 24.0 41.0 36 35.328734841060246 39.0 35.0 41.0 23.0 41.0 37 35.19629751546754 39.0 35.0 41.0 23.0 41.0 38 35.046941279448184 39.0 35.0 41.0 22.0 41.0 39 34.98129680474612 39.0 35.0 41.0 22.0 41.0 40 34.877344445525075 39.0 35.0 40.0 21.0 41.0 41 34.69915162306329 38.0 34.0 40.0 21.0 41.0 42 34.67240605385024 38.0 34.0 40.0 20.0 41.0 43 34.64509190750148 38.0 34.0 40.0 20.0 41.0 44 34.53052361465433 38.0 34.0 40.0 20.0 41.0 45 34.44768716287491 38.0 34.0 40.0 20.0 41.0 46 34.297036665743974 38.0 34.0 40.0 20.0 41.0 47 34.256809833391934 38.0 34.0 40.0 20.0 41.0 48 34.216081755407096 38.0 34.0 40.0 20.0 41.0 49 34.13496225695198 37.0 34.0 40.0 20.0 41.0 50 34.04602482288074 37.0 34.0 40.0 20.0 41.0 51 32.48267335991681 35.0 31.0 39.0 15.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 1.0 9 19.0 10 26.0 11 23.0 12 14.0 13 12.0 14 27.0 15 33.0 16 55.0 17 102.0 18 201.0 19 256.0 20 460.0 21 726.0 22 971.0 23 1526.0 24 2304.0 25 3654.0 26 5331.0 27 6316.0 28 6377.0 29 5811.0 30 5725.0 31 6272.0 32 8167.0 33 11470.0 34 19259.0 35 20233.0 36 22627.0 37 35132.0 38 59809.0 39 44392.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.47621327627612 19.5388540178204 22.160293864603826 19.824638841299645 2 40.611744110363816 21.63510814187496 21.933237074221758 15.819910673539466 3 31.008027411402967 19.956309335886942 33.55577666888611 15.479886583823982 4 27.69045463727023 22.792087800279802 31.261642739045538 18.25581482340443 5 22.3656549484914 27.65566669409802 31.527602175555675 18.45107618185491 6 24.122259046735543 31.496928935339312 31.46999633417373 12.910815683751412 7 72.44832307151354 6.055720559300351 17.40781195059364 4.0881444185924725 8 73.74595075822754 5.385398041401393 16.846342029072247 4.022309171298825 9 67.82788571599572 7.655217817411927 19.080625734100416 5.436270732491939 10 38.18743594155626 23.45118840102643 25.28634591933686 13.075029738080454 11 29.10217181503288 24.289465612305207 30.381470370398077 16.226892202263834 12 27.663896100009726 21.289473093583307 33.93021463786873 17.116416168538233 13 23.69956683399792 22.756925793202512 34.541435058765444 19.00207231403413 14 19.65668414791983 25.81340196159112 34.67946463973905 19.85044925075 15 19.658928531350295 24.3579193069344 38.049780424487714 17.93337173722759 16 22.062289121473512 22.09184016997464 37.739307383273356 18.10656332527849 17 21.835232331091444 22.10680272617774 34.84367869406809 21.214286248662724 18 21.610045860234763 23.630365011558574 35.508016189485815 19.25157293872085 19 23.636724097944892 24.014528642073213 33.18881997800504 19.159927281976852 20 25.235473228246313 23.95393028945065 32.943808120179256 17.866788362123785 21 24.12749594140663 23.647197887287064 33.48208607958584 18.74322009172047 22 21.969147209109206 23.298570327754796 33.55876918012674 21.173513283009267 23 22.084358891873087 23.912035132081964 34.08096239161498 19.922643584429963 24 21.0347355742255 23.545826569011048 35.42609619427383 19.99334166248962 25 21.944833055279165 23.214405949112347 33.73906798237411 21.10169301323438 26 20.46690656631779 25.37873970389101 33.9257258710078 20.228627858783394 27 21.149573193084308 25.074625749062967 34.361510320423136 19.41429073742958 28 19.742344782182588 25.443826823374504 34.79879102545879 20.015037368984117 29 21.05156844995399 24.54008842870716 34.32485205772554 20.083491063613305 30 21.819895710983264 23.353183657896114 34.14380512766801 20.68311550345261 31 22.02450866706068 25.226121630619375 32.505779287333446 20.243590414986496 32 22.440093665601832 25.87587063373907 32.562262937000156 19.121772763658942 33 22.19021897701003 25.209288754890885 31.75129239079204 20.84919987730704 34 20.738476961404086 25.398191026955047 32.832337076466146 21.030994935174725 35 21.21316405694749 25.043952508846612 31.618499704489516 22.124383729716385 36 22.332737324844576 26.8514292981813 30.76451180919748 20.05132156777664 37 22.27700180298802 27.06165321283488 30.2928172248947 20.368527759282394 38 21.435358016563548 26.56040758003097 30.29730599175563 21.706928411649844 39 22.360043989915237 24.859539003643384 30.867005319188728 21.91341168725265 40 22.462537499906485 24.4129067009808 32.713758818556556 20.41079698055616 41 20.21067279133967 25.879985336694922 32.05203977047439 21.85730210149102 42 20.788975588589555 26.043451263213807 31.46662975902803 21.700943389168607 43 22.155056969932744 24.69981371617527 31.37535816618911 21.769771147702873 44 21.469771895830682 25.802928172248947 30.00516208189007 22.7221378500303 45 21.741716354822056 25.52163211563063 30.00254363455453 22.734107894992782 46 21.372515280510523 26.004922680990823 30.533340315859565 22.089221722639095 47 21.030620871269647 25.668639230326107 32.180343689915986 21.120396208488255 48 20.777753671437228 24.94819214914676 32.48109106959833 21.79296310981768 49 20.848077685591807 25.1490644661734 31.94206498238159 22.0607928658532 50 19.911421667277637 24.930985209513192 31.96675320011671 23.19083992309246 51 19.666783873356923 25.276246193899766 31.15503452609844 23.901935406644874 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 170.0 1 507.0 2 844.0 3 5255.0 4 9666.0 5 6579.0 6 3492.0 7 3294.5 8 3097.0 9 2966.5 10 2836.0 11 2645.5 12 2455.0 13 2330.0 14 2205.0 15 2075.5 16 1946.0 17 1851.0 18 1756.0 19 1657.5 20 1559.0 21 1542.0 22 1525.0 23 1573.0 24 1621.0 25 1890.0 26 2094.0 27 2029.0 28 2508.0 29 2987.0 30 3499.5 31 4012.0 32 4700.5 33 5389.0 34 5737.5 35 6086.0 36 6617.0 37 7148.0 38 7889.0 39 8630.0 40 9823.0 41 11016.0 42 11921.5 43 12827.0 44 14245.5 45 15664.0 46 16303.5 47 16943.0 48 18427.5 49 19912.0 50 19866.5 51 19821.0 52 18407.0 53 16993.0 54 16239.5 55 15486.0 56 14447.5 57 13409.0 58 13123.5 59 12838.0 60 12353.0 61 11868.0 62 10612.5 63 9357.0 64 7840.0 65 6323.0 66 5388.0 67 4453.0 68 3843.0 69 3233.0 70 2548.0 71 1863.0 72 1621.0 73 1379.0 74 1223.5 75 815.5 76 563.0 77 456.0 78 349.0 79 253.5 80 158.0 81 114.5 82 71.0 83 59.0 84 47.0 85 39.0 86 31.0 87 28.0 88 25.0 89 21.0 90 17.0 91 10.5 92 4.0 93 4.0 94 4.0 95 2.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 267334.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.4917986690311 #Duplication Level Percentage of deduplicated Percentage of total 1 74.84399999997714 28.060361795841064 2 7.565999999997689 5.673258974596053 3 3.068999999999063 3.4518699034566382 4 1.9739999999993971 2.960352422905791 5 1.5240000000298914 2.856875058636204 6 1.318999999999787 2.9671009466666414 7 1.1439999999996517 3.0023432374150962 8 0.9709999999997033 2.912362920609446 9 0.8889999999997283 2.999718811508262 >10 6.61299999999798 40.17696128969484 >50 0.06399999999998045 1.5562845627510056 >100 0.021999999999993282 1.39282032055408 >500 0.0 0.0 >1k 0.0 0.0 >5k 9.999999999996945E-4 1.9896897553648725 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5307 1.985157144246523 No Hit GCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC 409 0.15299213717671528 Illumina Paired End PCR Primer 2 (95% over 24bp) GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 268 0.10024912656078164 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.11147104371310795 0.0 2 0.0 0.0 0.0 0.47730554287894544 0.0 3 0.0 0.0 0.0 0.636656766441979 0.0 4 0.0 0.0 0.0 0.8203221438350528 0.0 5 0.0 0.0 0.0 1.3084755399612469 0.0 6 0.0 0.0 0.0 1.6069785362131266 0.0 7 0.0 0.0 0.0 1.9208181525731856 0.0 8 0.0 0.0 0.0 2.4542332812137624 0.0 9 0.0 0.0 0.0 2.6674497071079624 0.0 10 0.0 0.0 0.0 3.143633058271675 0.0 11 0.0 0.0 0.0 3.8603395004002485 0.0 12 0.0 0.0 0.0 4.418816910681021 0.0 13 0.0 0.0 0.0 4.640262742486926 0.0 14 0.0 0.0 0.0 4.7277936962750715 0.0 15 0.0 0.0 0.0 4.912955329288455 0.0 16 0.0 0.0 0.0 5.305348365714798 0.0 17 0.0 0.0 0.0 5.737018112174284 0.0 18 0.0 0.0 0.0 6.243874703554355 0.0 19 0.0 0.0 0.0 6.5247966962675905 0.0 20 0.0 0.0 0.0 6.805718688980826 0.0 21 0.0 0.0 0.0 7.131528350303366 0.0 22 0.0 0.0 0.0 7.476041206879783 0.0 23 0.0 0.0 0.0 7.833646300133915 0.0 24 0.0 0.0 0.0 8.15421906678537 0.0 25 0.0 0.0 0.0 8.405964074902556 0.0 26 0.0 0.0 0.0 8.660327530355286 0.0 27 0.0 0.0 0.0 8.950227056790382 0.0 28 0.0 0.0 0.0 9.194490786806018 0.0 29 0.0 0.0 0.0 9.47728309904464 0.0 30 0.0 0.0 0.0 9.81543686923474 0.0 31 0.0 0.0 0.0 10.131520869025264 0.0 32 0.0 0.0 0.0 10.43974952680916 0.0 33 0.0 0.0 0.0 10.744611609447357 0.0 34 0.0 0.0 0.0 11.0303964329266 0.0 35 0.0 0.0 0.0 11.348350752242514 0.0 36 0.0 0.0 0.0 11.68538233071738 0.0 37 0.0 0.0 0.0 12.024658292622712 0.0 38 0.0 0.0 0.0 12.345979187084321 0.0 39 0.0 0.0 0.0 12.747349757232525 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGTAC 20 7.023563E-4 45.000004 6 ACGTTGG 20 7.023563E-4 45.000004 2 TGTATCG 20 7.023563E-4 45.000004 44 GTTAACG 20 7.023563E-4 45.000004 1 AACGCCG 20 7.023563E-4 45.000004 13 ATGGTCG 20 7.023563E-4 45.000004 44 ATCGCCA 20 7.023563E-4 45.000004 24 CGCTTTA 20 7.023563E-4 45.000004 19 GGTAGCG 20 7.023563E-4 45.000004 1 CGCCAGT 20 7.023563E-4 45.000004 26 ACACGCG 85 0.0 45.0 36 TGTTGCG 35 1.2072451E-7 45.0 1 TCTAAAC 25 3.8827482E-5 45.0 34 GCTACGA 50 2.1827873E-11 45.0 10 CTCACGA 30 2.1592314E-6 44.999996 24 CGACGGT 30 2.1592314E-6 44.999996 28 CGTGCGG 30 2.1592314E-6 44.999996 2 TACGAGG 30 2.1592314E-6 44.999996 2 CGGTCTA 30 2.1592314E-6 44.999996 31 CGTTTTT 2770 0.0 43.05054 1 >>END_MODULE