FastQCFastQC Report
Sat 18 Jun 2016
SRR3552048_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552048_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences570122
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116522.0437730871638No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTC107991.8941559876657978No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCG65141.142562469085564No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGC63761.118357123563027No Hit
GAACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT34510.6053090391179431No Hit
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC33770.5923293610841188TruSeq Adapter, Index 13 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCT15340.26906521762008834No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTC14980.2627507796576873No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT13600.23854543413515003No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTA11900.20872725486825627No Hit
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT6280.11015186223299574TruSeq Adapter, Index 16 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAT207.030291E-445.00000429
GCGTTAG207.030291E-445.0000041
TAGCCGT207.030291E-445.00000444
CTAGGCG207.030291E-445.0000041
TAGGCGT253.888324E-545.01
AGTACGG351.2104829E-745.02
CGTTTTT68650.043.6234551
ACGGCTG6800.042.352948
TACGGCT6750.042.3333367
TGATACC8150.041.1349684
GAATGAT23250.039.8709641
ATGATAC21400.039.427573
CGCACGG403.4541335E-739.3750042
GAATCTG14600.039.2979431
ATACGGC12550.039.2629476
CGGTTTT1900.039.078951
TCATGCA750.039.024
GATACCT8550.038.947375
AATGATA22000.038.6590922
CTATGCG356.24275E-638.5714261
Produced by FastQC (version 0.11.5)