##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552048_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	570122
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.601250258716554	31.0	30.0	33.0	28.0	33.0
2	30.39243004128941	31.0	30.0	33.0	26.0	34.0
3	29.562747973240814	31.0	28.0	33.0	25.0	34.0
4	33.99436261010801	35.0	33.0	37.0	30.0	37.0
5	34.834109190664456	35.0	35.0	37.0	32.0	37.0
6	34.89369643690298	35.0	35.0	37.0	32.0	37.0
7	35.41112077765811	37.0	35.0	37.0	33.0	37.0
8	35.06297073258004	37.0	35.0	37.0	32.0	37.0
9	37.01268149624116	39.0	37.0	39.0	32.0	39.0
10	36.33482833498795	38.0	35.0	39.0	32.0	39.0
11	36.46931183150273	38.0	35.0	39.0	32.0	39.0
12	36.80976878632994	39.0	35.0	39.0	33.0	39.0
13	36.72317328571779	39.0	35.0	39.0	32.0	39.0
14	37.900140671645715	40.0	37.0	41.0	33.0	41.0
15	37.949645865270945	40.0	37.0	41.0	33.0	41.0
16	37.78168707750271	39.0	37.0	41.0	33.0	41.0
17	37.48774648233185	39.0	36.0	41.0	32.0	41.0
18	37.310668593739585	39.0	36.0	40.0	32.0	41.0
19	36.79535257365967	38.0	36.0	40.0	32.0	41.0
20	36.620268995057195	38.0	35.0	40.0	31.0	41.0
21	36.89491547423183	38.0	35.0	40.0	32.0	41.0
22	37.20971300879461	39.0	35.0	40.0	33.0	41.0
23	37.29937276582907	39.0	35.0	40.0	33.0	41.0
24	37.218856665766275	39.0	35.0	41.0	33.0	41.0
25	36.65470723809992	38.0	35.0	40.0	31.0	41.0
26	36.767904062639225	38.0	35.0	40.0	32.0	41.0
27	37.026517131420995	39.0	35.0	40.0	33.0	41.0
28	36.92927654081056	39.0	35.0	40.0	32.0	41.0
29	36.857163905269395	39.0	35.0	41.0	32.0	41.0
30	36.538591740013544	39.0	35.0	40.0	31.0	41.0
31	36.54544816723438	39.0	35.0	40.0	31.0	41.0
32	36.229577178217994	39.0	35.0	40.0	30.0	41.0
33	35.78940823192229	39.0	35.0	41.0	27.0	41.0
34	35.533194649566234	39.0	35.0	41.0	24.0	41.0
35	35.300700551811715	39.0	35.0	41.0	22.0	41.0
36	35.12015323036122	39.0	35.0	41.0	21.0	41.0
37	35.05290271205111	39.0	35.0	41.0	21.0	41.0
38	34.85655701762079	39.0	35.0	40.0	20.0	41.0
39	34.77596198708347	39.0	35.0	40.0	18.0	41.0
40	34.585432591620744	39.0	34.0	40.0	18.0	41.0
41	34.38743286524639	38.0	34.0	40.0	18.0	41.0
42	34.38996565647353	38.0	34.0	40.0	18.0	41.0
43	34.32791227140858	38.0	34.0	40.0	18.0	41.0
44	34.29945871234578	38.0	34.0	40.0	17.0	41.0
45	34.2350128568973	38.0	34.0	40.0	18.0	41.0
46	34.00443063063695	38.0	33.0	40.0	17.0	41.0
47	33.920815193940946	38.0	33.0	40.0	17.0	41.0
48	33.88270229880622	38.0	33.0	40.0	17.0	41.0
49	33.82067522389944	38.0	33.0	40.0	15.0	41.0
50	33.727410975194786	38.0	33.0	40.0	15.0	41.0
51	31.99984213905094	35.0	30.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	14.0
9	34.0
10	37.0
11	16.0
12	20.0
13	25.0
14	32.0
15	49.0
16	103.0
17	219.0
18	423.0
19	710.0
20	1063.0
21	1578.0
22	2412.0
23	3323.0
24	5676.0
25	9161.0
26	12878.0
27	14731.0
28	14184.0
29	13093.0
30	13354.0
31	14658.0
32	18377.0
33	24719.0
34	36759.0
35	42227.0
36	51806.0
37	76486.0
38	122186.0
39	89767.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.030516275463846	15.22060190625866	18.477448686421504	25.271433131855986
2	35.39523119612995	27.01895383795047	22.017918971728857	15.567895994190717
3	27.40325754838438	25.64503737796472	33.313220679082725	13.638484394568179
4	24.575967950719317	20.863253829882026	38.06097642259025	16.499801796808402
5	24.79486846674922	24.64367275776062	32.33834161810981	18.22311715738035
6	21.723069799095633	32.443757651871	32.86226456793459	12.970907981098783
7	68.80404544992125	4.855802793086392	22.424849418194704	3.915302338797661
8	68.85613956311106	7.177235749541326	19.256930972669007	4.709693714678612
9	63.414848050066475	6.898523473923126	20.104819670175857	9.581808805834541
10	31.578679651022064	29.02501569839438	26.65429504562181	12.742009604961746
11	24.291116638193227	22.138068694068988	36.70460006805561	16.866214599682174
12	21.58625697657694	20.389320180592925	39.533292874156764	18.491129968673373
13	19.687365160439345	21.849884761507184	41.97066592764356	16.492084150409912
14	18.45412034617152	25.527694072496764	36.74634551902926	19.271840062302452
15	17.20421243172514	23.893307046561965	41.80473653007602	17.097743991636875
16	20.261628212908818	24.970971125478407	35.491877177165584	19.275523484447188
17	20.003262459613907	23.732288878520738	36.73722466419468	19.527223997670674
18	20.07938651727174	23.296417258060558	37.12556259888235	19.49863362578536
19	19.673859279241988	24.990616043583653	35.02723978376558	20.30828489340878
20	21.00129445978229	25.01745240492386	37.50267486608129	16.478578269212555
21	20.928853824269193	25.8085111607691	36.45903859174001	16.8035964232217
22	20.30214585650089	21.3226291916467	37.082238538418096	21.292986413434317
23	19.357961980067422	24.95781604639007	37.30780429451942	18.376417679023085
24	20.793619611241102	22.925619428824007	35.97475628023476	20.306004679700134
25	18.78790855290622	27.34327038774157	34.468061221984065	19.400759837368142
26	18.822813362753937	24.62209842805575	35.41698092688933	21.13810728230098
27	20.969371467861265	24.28953802870263	34.68450612325081	20.056584380185292
28	17.455737543894116	24.546500573561445	37.04066848849895	20.957093394045483
29	20.03799186840711	25.074983950803514	34.27126123882257	20.615762941966807
30	21.059527609879993	24.895373270984106	35.280168104370645	18.76493101476526
31	21.015852747306717	23.359386236630055	32.50479721884088	23.119963797222347
32	20.551039952852197	25.63959994527487	33.04818968571639	20.761170416156542
33	19.059780187398488	25.609957167062486	32.75246350781061	22.577799137728416
34	19.139412266146543	25.547338990602007	33.11536828959415	22.197880453657287
35	19.53003041454285	25.358256653839	33.48528911355815	21.626423818059994
36	19.44162828306924	27.671971963895448	32.72194372432567	20.164456028709647
37	20.41931376091433	25.113396781741454	33.023282735975805	21.444006721368407
38	19.337966259853154	27.37063295224531	30.340874409336948	22.950526378564586
39	21.187921181782148	24.8701155191345	30.960390933870297	22.98157236521306
40	20.6297950263277	26.0840662174061	33.51931691813331	19.76682183813289
41	18.577427287492853	26.726384879025893	31.96175555407439	22.734432279406864
42	20.058338390730405	24.880814983459683	34.50612325081298	20.554723374996932
43	21.889700800881215	24.399865291990135	31.85230529605944	21.858128611069212
44	20.501401454425544	24.678928369717358	31.43221977050526	23.387450405351835
45	19.987476364707906	24.00521291934007	30.861640140180523	25.1456705757715
46	22.602706087469	25.763257688705227	30.553109685295425	21.08092653853035
47	18.87245186118059	24.34654337141875	35.83706645244351	20.94393831495715
48	19.930295620937272	25.072703737094866	31.872827219437244	23.124173422530617
49	19.771733067659202	23.16995309775802	35.45083333040998	21.60748050417279
50	19.90100364483391	23.38639799902477	33.85170191643192	22.860896439709396
51	19.233427231364512	23.054013000726158	31.70935343663286	26.003206331276463
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	130.0
1	558.5
2	987.0
3	11158.5
4	21330.0
5	14610.5
6	7891.0
7	7285.0
8	6679.0
9	6524.5
10	6370.0
11	6214.0
12	6058.0
13	5755.0
14	5452.0
15	5151.0
16	4850.0
17	4495.0
18	4140.0
19	3811.0
20	3482.0
21	3518.0
22	3554.0
23	3658.5
24	3763.0
25	3988.5
26	4876.5
27	5539.0
28	6280.0
29	7021.0
30	8439.5
31	9858.0
32	10724.0
33	11590.0
34	13086.0
35	14582.0
36	16207.0
37	17832.0
38	19516.5
39	21201.0
40	22780.0
41	24359.0
42	26584.5
43	28810.0
44	31291.5
45	33773.0
46	52435.5
47	71098.0
48	57480.5
49	43863.0
50	44406.0
51	44949.0
52	39496.0
53	34043.0
54	30946.5
55	27850.0
56	25251.5
57	22653.0
58	20672.5
59	18692.0
60	16819.5
61	14947.0
62	13183.5
63	11420.0
64	9903.0
65	8386.0
66	6937.5
67	5489.0
68	4716.5
69	3944.0
70	3471.5
71	2999.0
72	2612.5
73	2226.0
74	1940.0
75	1344.5
76	1035.0
77	824.0
78	613.0
79	493.5
80	374.0
81	290.5
82	207.0
83	151.5
84	96.0
85	73.5
86	51.0
87	40.0
88	29.0
89	24.0
90	19.0
91	15.0
92	11.0
93	8.5
94	6.0
95	3.5
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	570122.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.95942713292772
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.48155284923367	29.017586309770245
2	8.112385882803588	6.321078133505559
3	3.653154973717522	4.269744750115208
4	2.3760014121050044	3.7027061553055316
5	1.8294591732451835	3.563734067635596
6	1.4958529165177963	3.49665436215915
7	1.254935840061681	3.4224106992167753
8	1.0416887016885776	3.2466876054904183
9	0.8915458681202412	3.126070465622399
>10	4.790250143266276	28.57977632627086
>50	0.049084483466730916	1.2773478163948517
>100	0.018633924277827126	1.3097507343202883
>500	9.089719159915671E-4	0.2114157600078984
>1k	0.0027269157479747007	2.197378209127319
>5k	9.089719159915671E-4	2.282369469432002
>10k+	9.089719159915671E-4	3.975289135625902
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11652	2.0437730871638	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTC	10799	1.8941559876657978	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCG	6514	1.142562469085564	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGC	6376	1.118357123563027	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT	3451	0.6053090391179431	No Hit
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC	3377	0.5923293610841188	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCT	1534	0.26906521762008834	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTC	1498	0.2627507796576873	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT	1360	0.23854543413515003	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTA	1190	0.20872725486825627	No Hit
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT	628	0.11015186223299574	TruSeq Adapter, Index 16 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13628661935515557	0.0
2	0.0	0.0	0.0	1.031709002634524	0.0
3	0.0	0.0	0.0	1.2576255608448719	0.0
4	0.0	0.0	0.0	2.122878962748324	0.0
5	0.0	0.0	0.0	4.7249536064210815	0.0
6	0.0	0.0	0.0	5.467601671221248	0.0
7	0.0	0.0	0.0	6.129039047782755	0.0
8	0.0	0.0	0.0	6.973945927362915	0.0
9	0.0	0.0	0.0	7.246519166073226	0.0
10	0.0	0.0	0.0	8.943699769523015	0.0
11	0.0	0.0	0.0	9.616889016736769	0.0
12	0.0	0.0	0.0	11.433342337254132	0.0
13	0.0	0.0	0.0	11.790985087402346	0.0
14	0.0	0.0	0.0	11.987434268454821	0.0
15	0.0	0.0	0.0	12.41190482037178	0.0
16	0.0	0.0	0.0	12.817782860510558	0.0
17	0.0	0.0	0.0	13.26926517482223	0.0
18	0.0	0.0	0.0	13.756353903199667	0.0
19	0.0	0.0	0.0	14.14013141047004	0.0
20	0.0	0.0	0.0	14.451994485390847	0.0
21	0.0	0.0	0.0	14.809111032375526	0.0
22	0.0	0.0	0.0	15.198676774444769	0.0
23	0.0	0.0	0.0	15.585085297532808	0.0
24	0.0	0.0	0.0	15.908700243105862	0.0
25	0.0	0.0	0.0	16.193551555631952	0.0
26	0.0	0.0	0.0	16.482261691357287	0.0
27	1.754010545111397E-4	0.0	0.0	16.77798786926307	0.0
28	1.754010545111397E-4	0.0	0.0	17.093534366328612	0.0
29	1.754010545111397E-4	0.0	0.0	17.40294182648626	0.0
30	1.754010545111397E-4	0.0	0.0	17.78022949473972	0.0
31	1.754010545111397E-4	0.0	0.0	18.12138454576389	0.0
32	1.754010545111397E-4	0.0	0.0	18.4711342484591	0.0
33	1.754010545111397E-4	0.0	0.0	18.791416573996443	0.0
34	1.754010545111397E-4	0.0	0.0	19.113628311133407	0.0
35	1.754010545111397E-4	0.0	0.0	19.455309565321105	0.0
36	1.754010545111397E-4	0.0	0.0	19.771031463441158	0.0
37	1.754010545111397E-4	0.0	0.0	20.106222878611945	0.0
38	1.754010545111397E-4	0.0	0.0	20.45000894545378	0.0
39	1.754010545111397E-4	0.0	0.0	20.812212123019282	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCAT	20	7.030291E-4	45.000004	29
GCGTTAG	20	7.030291E-4	45.000004	1
TAGCCGT	20	7.030291E-4	45.000004	44
CTAGGCG	20	7.030291E-4	45.000004	1
TAGGCGT	25	3.888324E-5	45.0	1
AGTACGG	35	1.2104829E-7	45.0	2
CGTTTTT	6865	0.0	43.623455	1
ACGGCTG	680	0.0	42.35294	8
TACGGCT	675	0.0	42.333336	7
TGATACC	815	0.0	41.134968	4
GAATGAT	2325	0.0	39.870964	1
ATGATAC	2140	0.0	39.42757	3
CGCACGG	40	3.4541335E-7	39.375004	2
GAATCTG	1460	0.0	39.297943	1
ATACGGC	1255	0.0	39.262947	6
CGGTTTT	190	0.0	39.07895	1
TCATGCA	75	0.0	39.0	24
GATACCT	855	0.0	38.94737	5
AATGATA	2200	0.0	38.659092	2
CTATGCG	35	6.24275E-6	38.571426	1
>>END_MODULE