FastQCFastQC Report
Sat 18 Jun 2016
SRR3552047_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552047_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences560965
Sequences flagged as poor quality0
Sequence length42-51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102871.833804248036865No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTC90621.6154305527082795No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGC59401.0588895920422843No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCG55120.9825924968580927No Hit
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC30460.5429928783435687TruSeq Adapter, Index 13 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT29940.5337231378071716No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCCACTTT23090.41161213266424823No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTA14530.25901794229586517No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCT12490.22265203711461498No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTC11820.2107083329619495No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT10440.18610786769228027No Hit
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC6420.1144456427762873TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT6330.11284126460652626TruSeq Adapter, Index 16 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGT253.7286103E-545.32050344
GTAGCCG253.7286103E-545.32050343
CGTAAGC302.060313E-645.32050343
ACAACGG253.9290127E-544.920582
CGGCGAT950.044.9205831
ATGTACG207.091635E-444.920581
TACGCGG207.091635E-444.920582
ACGCTCT453.9108272E-1044.9205828
ATACGGA502.1827873E-1144.9205828
TATCGCG207.091635E-444.9205832
TGTAACG207.091635E-444.920581
CGTTTTT66150.044.071741
CGATGAA9600.042.11304519
CCGATGA9700.041.6788918
CCCCGCA603.6379788E-1241.54379740
TACTGCG603.6379788E-1241.177229
TACGGCT7100.040.491797
TACGAAT501.0986696E-940.42852412
GCTACGA501.0986696E-940.42852410
ACGGCTG7150.040.2086338
Produced by FastQC (version 0.11.5)