##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552047_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	560965
Sequences flagged as poor quality	0
Sequence length	42-51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.96485698751259	30.0	28.0	33.0	25.0	33.0
2	28.12277593076217	31.0	26.0	33.0	16.0	33.0
3	27.672997424081718	31.0	25.0	33.0	16.0	34.0
4	32.86845525121888	35.0	32.0	35.0	28.0	37.0
5	34.128963482570214	35.0	33.0	35.0	32.0	37.0
6	34.07528811957965	35.0	35.0	37.0	30.0	37.0
7	34.73084595295607	35.0	35.0	37.0	32.0	37.0
8	34.13682315295963	35.0	35.0	37.0	30.0	37.0
9	36.152004135730394	39.0	35.0	39.0	31.0	39.0
10	35.78131612489193	37.0	35.0	39.0	31.0	39.0
11	36.27188327257494	37.0	35.0	39.0	32.0	39.0
12	36.4618184735233	38.0	35.0	39.0	32.0	39.0
13	36.52580285757578	39.0	35.0	39.0	32.0	39.0
14	37.399855605964724	39.0	36.0	40.0	32.0	41.0
15	37.59718164234845	39.0	37.0	40.0	32.0	41.0
16	37.39692315919888	39.0	36.0	40.0	32.0	41.0
17	37.29149946966388	39.0	36.0	40.0	32.0	41.0
18	37.04048024386548	39.0	36.0	40.0	32.0	41.0
19	36.62183023896321	38.0	35.0	40.0	31.0	41.0
20	36.512359951155595	38.0	35.0	40.0	31.0	41.0
21	36.79337926608612	38.0	35.0	40.0	32.0	41.0
22	37.06291123332115	39.0	35.0	40.0	32.0	41.0
23	37.19173566978332	39.0	35.0	40.0	33.0	41.0
24	37.147923667251966	39.0	35.0	40.0	32.0	41.0
25	36.547923667251965	38.0	35.0	40.0	31.0	41.0
26	36.66645156114909	38.0	35.0	40.0	31.0	41.0
27	36.830281746633034	39.0	35.0	40.0	32.0	41.0
28	36.77698786911839	39.0	35.0	40.0	31.0	41.0
29	36.76810139670033	39.0	35.0	40.0	31.0	41.0
30	36.334660807715274	38.0	35.0	40.0	30.0	41.0
31	36.356054299287834	39.0	35.0	40.0	30.0	41.0
32	35.905753478381	39.0	35.0	40.0	29.0	41.0
33	35.60587380674374	39.0	35.0	40.0	25.0	41.0
34	35.398197748522634	39.0	35.0	40.0	23.0	41.0
35	35.13626696852745	39.0	35.0	41.0	21.0	41.0
36	35.01572468870607	39.0	35.0	41.0	20.0	41.0
37	34.95693135935397	39.0	35.0	41.0	19.0	41.0
38	34.83106432665139	39.0	35.0	40.0	20.0	41.0
39	34.78414339575553	39.0	35.0	40.0	18.0	41.0
40	34.6147923667252	39.0	34.0	40.0	18.0	41.0
41	34.53919585000847	39.0	34.0	40.0	18.0	41.0
42	34.606971914468815	39.0	35.0	40.0	18.0	41.0
43	34.55142106408502	39.0	35.0	40.0	18.0	41.0
44	34.59343700935386	39.0	35.0	40.0	18.0	41.0
45	34.560632846966065	39.0	34.0	40.0	18.0	41.0
46	34.41404644019085	38.0	34.0	40.0	18.0	41.0
47	34.32993901375293	38.0	34.0	40.0	18.0	41.0
48	34.28697886973114	38.0	34.0	40.0	18.0	41.0
49	34.331118812575646	38.0	34.0	40.0	18.0	41.0
50	34.23106431881905	38.0	34.0	40.0	18.0	41.0
51	33.395417848413835	37.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	5.0
10	8.0
11	8.0
12	8.0
13	21.0
14	32.0
15	50.0
16	100.0
17	168.0
18	358.0
19	759.0
20	1695.0
21	2774.0
22	3793.0
23	4754.0
24	6563.0
25	9169.0
26	12372.0
27	13396.0
28	12687.0
29	11603.0
30	12301.0
31	14657.0
32	18832.0
33	26099.0
34	37118.0
35	44131.0
36	57794.0
37	89032.0
38	125848.0
39	54822.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.04914745126701	15.384917062561835	18.106120702717636	24.459814783453513
2	35.421996024707425	26.844277272200586	21.674792544989437	16.058934158102556
3	27.35126077384507	25.780931074131185	32.54623728753131	14.321570864492436
4	24.44359273751482	20.898273510825096	38.06066332124108	16.59747043041901
5	26.803454760992217	24.235914896651305	31.094988100861908	17.865642241494566
6	21.664453219006532	33.706024440027456	31.39144153378553	13.238080807180483
7	67.76501207740233	4.821334664373	23.475083115702404	3.938570142522261
8	68.15416291568994	8.87131995757311	18.007540577397876	4.966976549339085
9	62.74740848359524	6.846238178852514	18.903496653088876	11.502856684463381
10	33.633114365423864	28.598575668713732	25.369675469949104	12.398634495913292
11	26.510923141372455	21.804568912498997	35.24658401148022	16.437923934648328
12	21.413813696041643	20.287183692387227	37.71500895777811	20.583993653793016
13	22.171436720651023	21.278332872817376	40.09893665380193	16.451293752729672
14	18.396334887203302	27.16978777642099	35.423065610153934	19.010811726221778
15	17.22888237234052	23.34673286212152	39.9930476945977	19.431337070940256
16	20.08841906357794	24.203292540532832	34.56418849660852	21.144099899280704
17	19.947412048880057	25.247386200565096	35.82166445321901	18.98353729733584
18	19.97932134803419	22.56112235166187	36.077830167657524	21.381726132646424
19	19.53312595259954	24.369078284741473	34.36756303869225	21.730232723966736
20	22.670933124169956	24.340556006167944	36.8527448236521	16.13576604601
21	21.066020161685667	26.783489165990748	35.32947688358454	16.821013788739048
22	20.409829490253404	21.080103036731344	38.168869715579405	20.341197757435847
23	21.05247207936324	24.516502812118404	36.15377073435954	18.277254374158815
24	20.201973385148804	24.507589600064176	35.382243098945565	19.90819391584145
25	18.67300098936654	28.096227037337446	33.76039503355825	19.470376939737776
26	18.513276229354773	24.41435740197695	36.382305491429946	20.69006087723833
27	22.04558216644532	23.92484379595875	34.44279054842993	19.58678348916599
28	17.307675166899898	26.229443904699938	36.03754244917241	20.425338479227758
29	19.821557494674355	24.49172408260765	35.432157086449244	20.25456133626875
30	20.55903666004118	26.420543171142587	34.424964124321484	18.595456044494753
31	20.80735874787197	23.395933792660863	31.572379738486354	24.22432772098081
32	21.861435205404973	25.76684819908551	32.095585286069536	20.276131309439982
33	20.70343069531967	25.220646564402415	31.492160829998305	22.583761910279605
34	19.382670933124167	25.536174271122086	31.982030964498676	23.09912383125507
35	20.82732434287344	25.31049174190903	32.64018254258287	21.222001372634654
36	19.444528624780514	28.661146417334415	31.38555881382974	20.508766144055333
37	20.78864100255809	25.242394801814733	32.10485502660594	21.86410916902124
38	19.694811619263234	27.15534837289314	29.473853092438922	23.675986915404703
39	20.924300090023443	25.47966450669828	29.989571541896552	23.606463861381727
40	20.426051536192098	26.002691790040377	32.3004108990757	21.27084577469183
41	18.83183442817288	26.074888807679624	31.103188255951796	23.990088508195697
42	20.19306017309458	24.803686504505627	33.35769611294822	21.64555720945157
43	21.389428930609487	24.93458051497499	31.357110356151768	22.318880198263756
44	21.007253244896383	24.43748235247569	31.18085992226996	23.374404480357967
45	20.0626947971951	23.836792098225445	30.590960510910442	25.50955259366901
46	22.573364243103303	25.9183456918459	30.00285957336604	21.505430491684756
47	18.937929273218746	25.612784990656927	34.721515322097666	20.727770414026658
48	19.817203121431152	25.12306057079962	31.37887188931473	23.6808644184545
49	20.109634963769746	23.160745791121652	35.00873159037241	21.72088765473619
50	20.07330579270431	23.81790812316668	33.148210428630264	22.960575655498744
51	19.07191557244522	23.66305952768481	31.454407789549787	25.810617110320184
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	110.0
1	488.5
2	867.0
3	9650.5
4	18583.0
5	12950.5
6	7055.0
7	6621.0
8	6291.0
9	6136.0
10	5991.0
11	5859.0
12	5727.0
13	5441.5
14	5156.0
15	4887.0
16	4618.0
17	4366.5
18	4118.0
19	3830.0
20	3529.0
21	3465.0
22	3411.0
23	3589.5
24	3768.0
25	3850.0
26	4609.0
27	5288.0
28	5903.0
29	6516.0
30	8113.5
31	9711.0
32	10551.0
33	11391.0
34	12885.0
35	14407.5
36	15900.5
37	17390.5
38	18986.0
39	20595.5
40	22152.0
41	23669.0
42	25685.5
43	27702.0
44	29604.5
45	31507.0
46	48230.5
47	64954.0
48	55005.5
49	45087.0
50	45090.5
51	44988.5
52	40231.5
53	35550.0
54	32771.5
55	29993.0
56	27290.5
57	24588.0
58	22241.0
59	19894.0
60	17740.5
61	15589.5
62	13644.5
63	11673.5
64	10260.5
65	8843.0
66	7193.5
67	5572.0
68	4829.0
69	4086.0
70	3575.5
71	3065.0
72	2652.5
73	2229.5
74	2011.0
75	1500.0
76	1197.0
77	945.0
78	693.0
79	532.5
80	368.0
81	285.0
82	206.5
83	137.5
84	68.0
85	45.0
86	22.0
87	15.0
88	8.0
89	7.0
90	6.0
91	4.5
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
42	4938.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	556027.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.06448862029034
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.47097612726408	30.9917704034024
2	8.03397768924927	6.598223707916863
3	3.585057236429233	4.416556262653134
4	2.2833715904099186	3.7506194676112945
5	1.8079507279910347	3.712128604781674
6	1.4513421061353335	3.575917284092558
7	1.186378330796492	3.4102613625046114
8	1.0003145839150236	3.2861925478311163
9	0.8032914571845702	2.968807761209901
>10	4.307957673754539	26.589239749084626
>50	0.04538224902536849	1.232304738630487
>100	0.018327446718059175	1.3410744019786442
>500	8.727355580028178E-4	0.22847005111207527
>1k	0.0030545744530098623	2.379134798913744
>5k	0.0013091033370042269	3.675948728245578
>10k+	4.363677790014089E-4	1.8433501300313084
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10287	1.833804248036865	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTC	9062	1.6154305527082795	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGC	5940	1.0588895920422843	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCG	5512	0.9825924968580927	No Hit
GCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC	3046	0.5429928783435687	TruSeq Adapter, Index 13 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCT	2994	0.5337231378071716	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCCACTTT	2309	0.41161213266424823	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTA	1453	0.25901794229586517	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCT	1249	0.22265203711461498	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTC	1182	0.2107083329619495	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTT	1044	0.18610786769228027	No Hit
CCTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGC	642	0.1144456427762873	TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGCCACTTTCGTATGCCGTCTTCTGCT	633	0.11284126460652626	TruSeq Adapter, Index 16 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11890224880340128	0.0
2	0.0	0.0	0.0	0.8911429411817137	0.0
3	0.0	0.0	0.0	1.0865205494103911	0.0
4	0.0	0.0	0.0	1.8348738334833723	0.0
5	0.0	0.0	0.0	3.9669141568547057	0.0
6	0.0	0.0	0.0	4.560534079666289	0.0
7	0.0	0.0	0.0	5.1527278885491965	0.0
8	0.0	0.0	0.0	5.88592871212999	0.0
9	0.0	0.0	0.0	6.141737898086333	0.0
10	0.0	0.0	0.0	7.6651841023949805	0.0
11	0.0	0.0	0.0	8.319948659898568	0.0
12	0.0	0.0	0.0	10.042159493016499	0.0
13	0.0	0.0	0.0	10.410453415097198	0.0
14	0.0	0.0	0.0	10.605831023325877	0.0
15	0.0	0.0	0.0	11.076805148271282	0.0
16	0.0	0.0	0.0	11.490556451828546	0.0
17	0.0	0.0	0.0	11.947447701728272	0.0
18	0.0	0.0	0.0	12.392930040198586	0.0
19	0.0	0.0	0.0	13.145561666057597	0.0
20	0.0	0.0	0.0	13.498168334922855	0.0
21	0.0	0.0	0.0	13.851131532270283	0.0
22	0.0	0.0	0.0	14.252404338951628	0.0
23	0.0	0.0	0.0	14.616954711969553	0.0
24	0.0	0.0	0.0	14.924282263599332	0.0
25	0.0	0.0	0.0	15.198096137905218	0.0
26	0.0	0.0	0.0	15.502393197436561	0.0
27	0.0	0.0	0.0	15.810790334512848	0.0
28	0.0	0.0	0.0	16.086208586988494	0.0
29	1.7826424108455965E-4	0.0	0.0	16.38872300410899	0.0
30	1.7826424108455965E-4	0.0	0.0	16.74132967297425	0.0
31	1.7826424108455965E-4	0.0	0.0	17.052400773666808	0.0
32	1.7826424108455965E-4	0.0	0.0	17.37898086333372	0.0
33	1.7826424108455965E-4	0.0	0.0	17.69147807795495	0.0
34	1.7826424108455965E-4	0.0	0.0	18.004331821058354	0.0
35	1.7826424108455965E-4	0.0	0.0	18.32663356893924	0.0
36	1.7826424108455965E-4	0.0	0.0	18.63948731204264	0.0
37	1.7826424108455965E-4	0.0	0.0	18.9655326089863	0.0
38	1.7826424108455965E-4	0.0	0.0	19.323130676601927	0.0
39	1.7826424108455965E-4	0.0	0.0	19.67823304484237	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCCGT	25	3.7286103E-5	45.320503	44
GTAGCCG	25	3.7286103E-5	45.320503	43
CGTAAGC	30	2.060313E-6	45.320503	43
ACAACGG	25	3.9290127E-5	44.92058	2
CGGCGAT	95	0.0	44.92058	31
ATGTACG	20	7.091635E-4	44.92058	1
TACGCGG	20	7.091635E-4	44.92058	2
ACGCTCT	45	3.9108272E-10	44.92058	28
ATACGGA	50	2.1827873E-11	44.92058	28
TATCGCG	20	7.091635E-4	44.92058	32
TGTAACG	20	7.091635E-4	44.92058	1
CGTTTTT	6615	0.0	44.07174	1
CGATGAA	960	0.0	42.113045	19
CCGATGA	970	0.0	41.67889	18
CCCCGCA	60	3.6379788E-12	41.543797	40
TACTGCG	60	3.6379788E-12	41.1772	29
TACGGCT	710	0.0	40.49179	7
TACGAAT	50	1.0986696E-9	40.428524	12
GCTACGA	50	1.0986696E-9	40.428524	10
ACGGCTG	715	0.0	40.208633	8
>>END_MODULE