FastQCFastQC Report
Sat 18 Jun 2016
SRR3552044_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552044_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260428
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79873.066874529620471No Hit
GAATCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTC40861.5689557190471068No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCG36091.3857956901715638No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGC35391.3589168599382555No Hit
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC16260.6243568279908458Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCT8520.3271537622682661No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCT7730.2968190824335325No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTC6180.23730167263120708No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTA5840.22424624080360023No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT3470.13324220129939945No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC3430.1317062681432104Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT3020.11596295329227271Illumina Single End Adapter 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTTAC207.023229E-445.018
CGTGACA207.023229E-445.042
GCTTGCG207.023229E-445.01
CGTTAGG207.023229E-445.02
ACGTGAC207.023229E-445.041
TAGGGTC253.8824714E-545.05
GCGATAT207.023229E-445.09
TATAGCG207.023229E-445.01
GAGCACG207.023229E-445.01
ACCCGAT207.023229E-445.041
CAACTAC207.023229E-445.016
CCCGATT207.023229E-445.042
CACGACC302.159015E-644.99999627
TATACTG600.044.99999618
AACACGT302.159015E-644.99999641
CGTTTTT34900.043.581661
ATAGGGC650.041.538464
TACGAAT650.041.5384612
CGAATAT650.041.5384614
GCTACGA650.041.5384610