##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552044_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	260428
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.621603667808376	31.0	30.0	33.0	28.0	33.0
2	30.426025619365046	31.0	30.0	33.0	26.0	34.0
3	29.650202743176617	31.0	28.0	33.0	25.0	34.0
4	34.037158062881105	35.0	33.0	37.0	32.0	37.0
5	34.87376165389282	35.0	35.0	37.0	33.0	37.0
6	34.94411123227917	35.0	35.0	37.0	32.0	37.0
7	35.43361696898951	37.0	35.0	37.0	33.0	37.0
8	34.91601517501958	37.0	35.0	37.0	32.0	37.0
9	36.878845592639806	39.0	37.0	39.0	32.0	39.0
10	36.33987128880151	38.0	35.0	39.0	32.0	39.0
11	36.491041669866526	38.0	35.0	39.0	32.0	39.0
12	36.70277773511297	39.0	35.0	39.0	33.0	39.0
13	36.547633127006314	39.0	35.0	39.0	32.0	39.0
14	37.68214631299246	40.0	36.0	41.0	33.0	41.0
15	37.74443224230882	40.0	36.0	41.0	33.0	41.0
16	37.567312270569985	39.0	36.0	41.0	33.0	41.0
17	37.27910593330978	39.0	36.0	41.0	32.0	41.0
18	37.11297940313638	39.0	36.0	40.0	32.0	41.0
19	36.53293808653447	37.0	35.0	40.0	31.0	41.0
20	36.37084338089606	38.0	35.0	40.0	31.0	41.0
21	36.595715514460814	38.0	35.0	40.0	32.0	41.0
22	36.861090205354266	38.0	35.0	40.0	32.0	41.0
23	36.96787211820541	38.0	35.0	40.0	33.0	41.0
24	36.850941527024744	38.0	35.0	40.0	32.0	41.0
25	36.32370559233262	38.0	35.0	40.0	31.0	41.0
26	36.43334434085428	38.0	35.0	40.0	31.0	41.0
27	36.63049672078271	38.0	35.0	40.0	32.0	41.0
28	36.48854577848772	38.0	35.0	40.0	31.0	41.0
29	36.46254626998633	38.0	35.0	40.0	31.0	41.0
30	36.16049733515597	38.0	35.0	40.0	31.0	41.0
31	36.02973566590382	38.0	35.0	40.0	30.0	41.0
32	35.71164390925707	38.0	35.0	40.0	29.0	41.0
33	35.27559632604789	38.0	35.0	40.0	25.0	41.0
34	34.98203342190548	38.0	35.0	40.0	23.0	41.0
35	34.69024836039136	38.0	34.0	40.0	21.0	41.0
36	34.47610471992259	38.0	34.0	40.0	19.0	41.0
37	34.37080498256716	38.0	34.0	40.0	18.0	41.0
38	34.246732302210205	38.0	34.0	40.0	18.0	41.0
39	34.215660374460505	38.0	34.0	40.0	18.0	41.0
40	34.12922573609597	38.0	34.0	40.0	18.0	41.0
41	33.83603145591104	38.0	33.0	40.0	17.0	41.0
42	33.88670189073372	38.0	33.0	40.0	17.0	41.0
43	33.77553104888875	38.0	33.0	40.0	15.0	41.0
44	33.80629195017433	38.0	33.0	40.0	16.0	41.0
45	33.76304391232893	37.0	33.0	40.0	17.0	41.0
46	33.61689603268466	37.0	33.0	40.0	17.0	41.0
47	33.53939284562336	37.0	33.0	40.0	17.0	41.0
48	33.53134839571782	37.0	33.0	40.0	17.0	41.0
49	33.463083846591	37.0	33.0	40.0	17.0	41.0
50	33.37170734329642	37.0	33.0	40.0	15.0	41.0
51	31.750514537607323	35.0	30.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	10.0
10	14.0
11	13.0
12	7.0
13	11.0
14	13.0
15	22.0
16	49.0
17	115.0
18	202.0
19	362.0
20	558.0
21	831.0
22	1186.0
23	1751.0
24	2619.0
25	4170.0
26	6050.0
27	7082.0
28	7050.0
29	6693.0
30	6803.0
31	7628.0
32	9327.0
33	12945.0
34	20733.0
35	22073.0
36	23171.0
37	33320.0
38	50334.0
39	35278.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.32735343357857	14.298385734252845	17.62521695055831	26.749043881610273
2	37.7835716589614	26.198795828405547	20.921329503739997	15.096303008893052
3	28.014652802310042	24.207458491406452	34.08542860214723	13.692460104136266
4	24.947010306111476	20.273933678406316	38.92438601072081	15.854670004761392
5	25.143609750103675	23.387270186001505	33.128542245841466	18.340577818053358
6	21.564501512894157	32.38975839771453	33.978681247792096	12.067058841599215
7	65.15889228500775	4.90884236718018	25.228086073694072	4.70417927411799
8	64.01423810035787	8.989048796596371	21.630546638610287	5.366166464435468
9	59.1591533936443	6.925906584545441	23.377286620486277	10.537653401323976
10	35.53957331776921	20.93476891885665	29.696499608337046	13.829158155037094
11	28.372525227702088	20.829557497657703	34.21022317108759	16.587694103552614
12	26.320902514322576	18.07102154914218	37.43645076566268	18.171625170872563
13	21.301856943185832	20.785399419417267	40.433824319965595	17.478919317431306
14	19.338934369576236	24.223969772835485	36.214615939914296	20.222479917673983
15	17.283471823306247	21.81255471761869	43.63394105088547	17.270032408189596
16	19.56702044327031	21.802187168814413	38.318844363893284	20.311948024021994
17	19.82659314666626	21.28112184557728	38.48702904449598	20.40525596326048
18	19.816609581151027	21.971907782573304	37.96980355414932	20.241679082126346
19	20.723962093169707	23.704440382754544	35.82103306864085	19.750564455434898
20	22.820894834656798	22.307893160489655	38.77271261154714	16.098499393306405
21	21.12292073048981	25.726496382877418	36.218839756093814	16.93174313053896
22	19.979802478996113	21.549526164621316	37.17572611239959	21.294945243982983
23	20.2201760179397	24.525012671448536	37.24061928824858	18.014192022363186
24	22.42232018062574	20.62259050486123	36.18197736034528	20.773111954167756
25	18.99373339272275	25.45962799698957	35.25312178413996	20.293516826147727
26	18.703826009492065	21.81485861735297	37.24522708771714	22.23608828543782
27	19.867295375305265	25.30104289861305	34.78735005452563	20.044311671556052
28	16.95977391063941	24.669774371419358	38.15833934907153	20.212112368869704
29	19.982874345308492	22.610471992258894	35.25811356689757	22.148540095535044
30	19.383860414394764	25.658147357427	35.817961202328476	19.140031025849755
31	21.427419478704287	25.469995545793843	32.77258973689465	20.329995238607214
32	22.74601809329258	26.919916445236304	32.50072956824919	17.83333589322193
33	20.13032392830264	24.510421306464742	32.32524920515459	23.034005560078025
34	20.32653938900579	26.320134547744484	32.790636951479875	20.562689111769856
35	20.197137020596863	26.76363524659407	31.20939376718325	21.829833965625816
36	19.318967238545778	28.912405732102535	31.31422120509316	20.45440582425853
37	20.026264456970832	30.52743944583532	31.894419954843567	17.551876142350284
38	19.9675150137466	28.789531079607418	29.699955457938472	21.54299844870751
39	21.123304713778857	28.11640837390757	31.13874084199856	19.62154607031502
40	22.41848034773527	24.865221865544413	31.696668560984225	21.019629225736097
41	18.3206106870229	28.81717787641882	31.691292794937564	21.170918641620716
42	21.269218363616815	25.056445543489946	32.95997358194971	20.714362510943523
43	21.365598169167676	24.335708909948238	31.731227056998478	22.5674658638856
44	19.48676793585943	25.069500975317556	32.1977667531909	23.245964335632113
45	20.085013900195065	24.09187952140323	31.23588861412751	24.587217964274195
46	22.329012241387254	26.654967975793692	30.7393982213894	20.276621561429646
47	18.25763742761915	24.322653478120632	36.529098253644	20.890610840616215
48	19.993241894112767	24.68167785337982	32.80330840001843	22.521771852488982
49	19.438770024728523	23.35578355629963	36.119004100941524	21.08644231803032
50	19.648424900548328	23.578493863947042	33.929147403505	22.84393383199963
51	18.686162778195893	23.170319627689803	32.483066336953016	25.660451257161288
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	164.0
1	461.5
2	759.0
3	7305.0
4	13851.0
5	9076.5
6	4302.0
7	3862.5
8	3423.0
9	3332.5
10	3242.0
11	3062.5
12	2883.0
13	2685.0
14	2487.0
15	2304.5
16	2122.0
17	1978.5
18	1835.0
19	1650.0
20	1465.0
21	1534.5
22	1604.0
23	1577.0
24	1550.0
25	1663.0
26	1821.0
27	1866.0
28	2213.5
29	2561.0
30	3039.0
31	3517.0
32	4020.0
33	4523.0
34	5083.0
35	5643.0
36	6119.0
37	6595.0
38	6955.5
39	7316.0
40	8428.0
41	9540.0
42	10925.0
43	12310.0
44	20834.0
45	29358.0
46	23886.0
47	18414.0
48	18966.0
49	19518.0
50	17298.0
51	15078.0
52	14337.5
53	13597.0
54	12942.0
55	12287.0
56	11907.0
57	11527.0
58	10938.0
59	10349.0
60	9957.5
61	9566.0
62	8718.0
63	7870.0
64	6845.5
65	5821.0
66	4727.0
67	3633.0
68	3119.5
69	2606.0
70	2214.5
71	1823.0
72	1598.0
73	1373.0
74	1156.5
75	760.5
76	581.0
77	469.5
78	358.0
79	259.5
80	161.0
81	133.5
82	106.0
83	84.5
84	63.0
85	44.0
86	25.0
87	23.5
88	22.0
89	14.5
90	7.0
91	4.0
92	1.0
93	3.5
94	6.0
95	3.5
96	1.0
97	2.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	260428.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.20672124349148
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.33592627209777	28.783387346982657
2	7.647159325031909	5.843457692721213
3	3.2884091617169675	3.769179965287911
4	2.244198550768334	3.429738737770132
5	1.7256107978814283	3.2964965364707326
6	1.3587803137656906	3.1148724407513786
7	1.184912714445081	3.169014084507042
8	1.0210952653742174	3.121016173376135
9	0.8200922603792926	2.8199732747630826
>10	5.3024592717661125	31.129141259772375
>50	0.04020060099898493	1.016403766108099
>100	0.022110330549441715	1.416898336584392
>500	0.004020060099898494	1.0855207581366058
>1k	0.004020060099898494	4.938025097147772
>5k	0.0010050150249746234	3.066874529620471
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7987	3.066874529620471	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTC	4086	1.5689557190471068	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCG	3609	1.3857956901715638	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGC	3539	1.3589168599382555	No Hit
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	1626	0.6243568279908458	Illumina Single End Adapter 1 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCT	852	0.3271537622682661	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCT	773	0.2968190824335325	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTC	618	0.23730167263120708	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTA	584	0.22424624080360023	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	347	0.13324220129939945	No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	343	0.1317062681432104	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT	302	0.11596295329227271	Illumina Single End Adapter 1 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.8398328904726064E-4	0.0	0.0	0.13593008432273027	0.0
2	3.8398328904726064E-4	0.0	0.0	1.1381264687360806	0.0
3	3.8398328904726064E-4	0.0	0.0	1.345093461532554	0.0
4	3.8398328904726064E-4	0.0	0.0	1.8964934646044205	0.0
5	3.8398328904726064E-4	0.0	0.0	4.048719799714316	0.0
6	3.8398328904726064E-4	0.0	0.0	4.482620916337721	0.0
7	3.8398328904726064E-4	0.0	0.0	5.1772466862242155	0.0
8	3.8398328904726064E-4	0.0	0.0	5.911806718171625	0.0
9	3.8398328904726064E-4	0.0	0.0	6.100726496382878	0.0
10	3.8398328904726064E-4	0.0	0.0	7.970725114043037	0.0
11	3.8398328904726064E-4	0.0	0.0	8.632712304360513	0.0
12	3.8398328904726064E-4	0.0	0.0	10.725421229668084	0.0
13	3.8398328904726064E-4	0.0	0.0	11.019168445789239	0.0
14	3.8398328904726064E-4	0.0	0.0	11.187737109680986	0.0
15	3.8398328904726064E-4	0.0	0.0	11.618182376702967	0.0
16	3.8398328904726064E-4	0.0	0.0	12.039796028076857	0.0
17	3.8398328904726064E-4	0.0	0.0	12.466401462208365	0.0
18	3.8398328904726064E-4	0.0	0.0	12.902222495277005	0.0
19	7.679665780945213E-4	0.0	0.0	13.246655505552399	0.0
20	7.679665780945213E-4	0.0	0.0	13.530419156158324	0.0
21	7.679665780945213E-4	0.0	0.0	13.866788517363725	0.0
22	7.679665780945213E-4	0.0	0.0	14.215445343818637	0.0
23	7.679665780945213E-4	0.0	0.0	14.528775707681202	0.0
24	7.679665780945213E-4	0.0	0.0	14.811003425130938	0.0
25	7.679665780945213E-4	0.0	0.0	15.06865621208165	0.0
26	7.679665780945213E-4	0.0	0.0	15.313253567204756	0.0
27	7.679665780945213E-4	0.0	0.0	15.565530588108805	0.0
28	7.679665780945213E-4	0.0	0.0	15.798608444560493	0.0
29	7.679665780945213E-4	0.0	0.0	16.068932680049762	0.0
30	7.679665780945213E-4	0.0	0.0	16.35269633065569	0.0
31	7.679665780945213E-4	0.0	0.0	16.64836346322208	0.0
32	7.679665780945213E-4	0.0	0.0	16.9328950804061	0.0
33	0.001151949867141782	0.0	0.0	17.224722380082017	0.0
34	0.001151949867141782	0.0	0.0	17.46855176862703	0.0
35	0.001151949867141782	0.0	0.0	17.739259987405347	0.0
36	0.001151949867141782	0.0	0.0	18.005744390004146	0.0
37	0.001151949867141782	0.0	0.0	18.298723639547205	0.0
38	0.001151949867141782	0.0	0.0	18.616277819589293	0.0
39	0.001151949867141782	0.0	0.0	18.939591748967086	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTTTAC	20	7.023229E-4	45.0	18
CGTGACA	20	7.023229E-4	45.0	42
GCTTGCG	20	7.023229E-4	45.0	1
CGTTAGG	20	7.023229E-4	45.0	2
ACGTGAC	20	7.023229E-4	45.0	41
TAGGGTC	25	3.8824714E-5	45.0	5
GCGATAT	20	7.023229E-4	45.0	9
TATAGCG	20	7.023229E-4	45.0	1
GAGCACG	20	7.023229E-4	45.0	1
ACCCGAT	20	7.023229E-4	45.0	41
CAACTAC	20	7.023229E-4	45.0	16
CCCGATT	20	7.023229E-4	45.0	42
CACGACC	30	2.159015E-6	44.999996	27
TATACTG	60	0.0	44.999996	18
AACACGT	30	2.159015E-6	44.999996	41
CGTTTTT	3490	0.0	43.58166	1
ATAGGGC	65	0.0	41.53846	4
TACGAAT	65	0.0	41.53846	12
CGAATAT	65	0.0	41.53846	14
GCTACGA	65	0.0	41.53846	10
>>END_MODULE