FastQCFastQC Report
Sat 18 Jun 2016
SRR3552043_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552043_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258186
Sequences flagged as poor quality0
Sequence length42-51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73632.8518200057323013No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGC33291.2893805241182714No Hit
GAATCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTC31991.2390292269913938No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCG29031.1243831966101958No Hit
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC14560.5639345278210283Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAAACAGTT12760.4942173471838132No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTA7540.2920375233358896No Hit
GAACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCT7210.27925604021906686No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCT6130.23742573183673787No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTC4880.18901102306089407No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC3690.1429202203062908Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT3200.12394165446616004Illumina Single End Adapter 1 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT2960.11464603038119806No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGAA206.7793916E-445.32362742
CGATCGA206.7793916E-445.32362741
CCGATCG253.7214173E-545.32362440
TAGGGCA453.8926373E-1044.919825
GCGTCCC453.8926373E-1044.919827
ATAGGGC351.2240889E-744.9198154
ATTCGCG207.085089E-444.9198151
TACGGGT207.085089E-444.9198154
ACGGGTA302.1856104E-644.9198155
GCCGATA207.085089E-444.91981532
CTAGTAG207.085089E-444.9198151
GCGATTA207.085089E-444.9198159
GCGATAT207.085089E-444.9198159
CGTAAGG406.886694E-944.9198152
CGCCGGT207.085089E-444.91981528
GCGTACG207.085089E-444.9198151
GCGTAAG207.085089E-444.9198151
CGGCGAA1000.044.9198131
TACGGGA253.923481E-544.919814
ACGGGTC253.923481E-544.919815
Produced by FastQC (version 0.11.5)