##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552043_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	258186
Sequences flagged as poor quality	0
Sequence length	42-51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.999938029172768	30.0	28.0	33.0	25.0	33.0
2	28.208400145631444	31.0	27.0	33.0	16.0	33.0
3	27.855848109502453	31.0	25.0	33.0	16.0	34.0
4	32.92709132176028	35.0	32.0	35.0	28.0	37.0
5	34.15150705305477	35.0	33.0	35.0	32.0	37.0
6	34.114227727297376	35.0	35.0	37.0	30.0	37.0
7	34.70023548914349	35.0	35.0	37.0	32.0	37.0
8	33.864922962515394	35.0	35.0	37.0	28.0	37.0
9	35.97052899847397	39.0	35.0	39.0	30.0	39.0
10	35.70666496246892	37.0	34.0	39.0	31.0	39.0
11	36.21869504930554	37.0	35.0	39.0	32.0	39.0
12	36.26872487276614	37.0	35.0	39.0	32.0	39.0
13	36.26933683468507	38.0	35.0	39.0	32.0	39.0
14	37.112860495921545	39.0	36.0	40.0	32.0	41.0
15	37.33434423245257	39.0	36.0	40.0	32.0	41.0
16	37.12684266381601	39.0	35.0	40.0	32.0	41.0
17	37.07397767500949	38.0	35.0	40.0	32.0	41.0
18	36.772365658866086	38.0	35.0	40.0	32.0	41.0
19	36.325772892410896	37.0	35.0	40.0	31.0	41.0
20	36.18854236867995	37.0	35.0	40.0	31.0	41.0
21	36.43675489763194	38.0	35.0	40.0	31.0	41.0
22	36.6732859256505	38.0	35.0	40.0	32.0	41.0
23	36.82699294307205	38.0	35.0	40.0	32.0	41.0
24	36.766133717552464	38.0	35.0	40.0	32.0	41.0
25	36.210441309753435	38.0	35.0	40.0	31.0	41.0
26	36.3107875717506	38.0	35.0	40.0	31.0	41.0
27	36.40972399742821	38.0	35.0	40.0	31.0	41.0
28	36.31461427033224	38.0	35.0	40.0	31.0	41.0
29	36.32347609862657	38.0	35.0	40.0	31.0	41.0
30	35.9441720310164	38.0	35.0	40.0	30.0	41.0
31	35.82762427087449	38.0	35.0	40.0	30.0	41.0
32	35.34764472124747	38.0	35.0	40.0	26.0	41.0
33	35.032585035594494	38.0	35.0	40.0	23.0	41.0
34	34.774348725337546	38.0	35.0	40.0	22.0	41.0
35	34.49674265839356	38.0	34.0	40.0	19.0	41.0
36	34.3229725856553	38.0	34.0	40.0	18.0	41.0
37	34.26413128519749	38.0	34.0	40.0	18.0	41.0
38	34.16569449931445	38.0	34.0	40.0	18.0	41.0
39	34.16205758639121	38.0	34.0	40.0	18.0	41.0
40	34.14135545691865	38.0	34.0	40.0	18.0	41.0
41	33.839081127559204	38.0	33.0	40.0	18.0	41.0
42	33.93882317399084	38.0	34.0	40.0	16.0	41.0
43	33.861105357163794	38.0	34.0	40.0	15.0	41.0
44	33.949840344246724	38.0	34.0	40.0	17.0	41.0
45	33.922653920262015	38.0	34.0	40.0	18.0	41.0
46	33.81224327081289	37.0	33.0	40.0	18.0	41.0
47	33.73451026525914	37.0	33.0	40.0	18.0	41.0
48	33.72011584324424	37.0	33.0	40.0	18.0	41.0
49	33.741693797071086	37.0	33.0	40.0	18.0	41.0
50	33.54530744968987	37.0	33.0	40.0	15.0	41.0
51	32.810484517104854	36.0	32.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	10.0
12	12.0
13	14.0
14	17.0
15	22.0
16	50.0
17	91.0
18	249.0
19	477.0
20	879.0
21	1317.0
22	1767.0
23	2318.0
24	3220.0
25	4630.0
26	6029.0
27	6803.0
28	6612.0
29	6220.0
30	6585.0
31	7617.0
32	9861.0
33	14140.0
34	20866.0
35	22760.0
36	26263.0
37	38123.0
38	50555.0
39	20674.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.268364667332854	14.477547194658113	17.075674126404994	26.17841401160404
2	37.57678572811849	26.468127628918687	20.530160426979	15.424926215983826
3	27.76447987110068	24.82435143656124	33.15710379338926	14.25406489894882
4	24.559038832469614	20.066928493411726	39.25116001642227	16.122872657696387
5	27.350049963979455	22.811074186826552	31.96300341614185	17.87587243305214
6	21.626269433664103	33.393754889885585	32.43746756214512	12.542508114305189
7	63.50228130107751	4.946821283880613	26.801995460636906	4.748901954404964
8	62.82098951918385	10.661306190111006	20.574701959052778	5.943002331652374
9	57.941174192249	6.779221181628749	22.375729125514166	12.90387550060809
10	37.63527069631971	20.506921366766594	28.484503420015027	13.37330451689867
11	30.679045339406475	20.48949207160729	32.77211002920375	16.059352559782482
12	25.777927540610257	17.707001928841997	35.80984251663529	20.70522801391245
13	23.894014392724625	20.264073187546963	38.46451782823236	17.377394591496053
14	19.067261586608105	25.95493171589474	35.278442673111634	19.69936402438552
15	17.021449652576052	21.03057485688612	41.72728188205402	20.220693608483806
16	19.06997281029955	21.033673398247775	37.53340614905533	22.36294764239734
17	19.7613348516186	23.099238533460372	37.26576963894247	19.873656975978555
18	19.666442022417947	21.077827612651344	36.8490158258	22.406714539130704
19	20.397310466098084	23.045014059631427	34.93876507634031	21.618910397930176
20	24.403337129046502	21.528278063101794	38.008257612728805	16.060127195122895
21	21.266064000371827	26.638934721479863	35.14326880620948	16.951732471938836
22	19.97590884091314	21.123143780065533	38.46490514590257	20.43604223311876
23	21.960911900722735	23.747608313386472	36.079028297428984	18.212451488461806
24	21.567397147792676	22.15108487679425	35.86677821415569	20.41473976125739
25	18.863532492079354	26.047500639074155	34.46236434198601	20.62660252686048
26	18.21671198283408	21.735880334332613	38.59194534173038	21.455462341102926
27	21.183952654287992	24.673297545180606	34.36088711239184	19.781862688139558
28	16.697264762613003	26.508795984290394	37.17939779848636	19.614541454610244
29	19.659470304354226	22.225062551803738	36.55233049042164	21.563136653420404
30	19.171837357563927	27.18001750675869	34.86091422462876	18.787230911048624
31	21.21183952654288	24.673297545180606	32.13148660268178	21.983376325594726
32	23.938943242468607	26.387952871185888	31.617903371987637	18.055200514357868
33	21.562749335750194	24.211227564623954	31.38318886384235	22.842834235783506
34	20.23115118557939	26.107922195626408	31.61829068965784	22.04263592913636
35	21.443842810996724	26.108309513296618	30.863408550424886	21.584439125281772
36	19.53126815551579	29.489980091871754	29.836629406706795	21.142122345905666
37	20.121927602581085	30.093808339724077	31.41882208950137	18.365441968193473
38	20.423648067672143	28.47559511360027	28.876856219934467	22.22390059879312
39	20.925224450589887	28.386899367122925	30.443168878250564	20.244707304036623
40	21.941933334882606	24.7647045153494	30.423802994740225	22.869559155027773
41	18.630754572285095	27.918632303842966	30.911048623860317	22.53956450001162
42	21.473666271602642	24.732944466392446	31.871983763643264	21.921405498361647
43	20.5477149880991	24.55102926175336	31.22100499095219	23.68025075919535
44	19.49089942664629	25.270164111262666	31.914344786076924	23.324591676014116
45	20.296799459085527	24.383361408253638	30.810629125743073	24.509210006917765
46	22.197425966239745	27.00507693570387	30.198582835345476	20.598914262710906
47	18.13353239819747	25.49176707847559	35.411919660130614	20.962780863196322
48	19.71015738891516	25.431969452402264	32.257888010380555	22.59998514830202
49	20.559830846976702	23.37149177489516	35.15944079448767	20.909236583640464
50	19.80278508420522	23.75333674661831	33.48823393769322	22.955644231483255
51	18.558369127228243	23.452785279622297	32.41578500994673	25.57306058320273
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	126.0
1	387.0
2	648.0
3	6444.5
4	12323.5
5	8187.5
6	3905.5
7	3631.0
8	3405.0
9	3300.5
10	3211.0
11	2980.0
12	2749.0
13	2618.0
14	2487.0
15	2309.0
16	2131.0
17	1960.5
18	1787.5
19	1618.5
20	1448.5
21	1487.5
22	1530.0
23	1512.0
24	1494.0
25	1573.5
26	1794.5
27	1937.0
28	2230.5
29	2523.0
30	2937.5
31	3352.0
32	3819.0
33	4286.0
34	4873.5
35	5467.5
36	5966.5
37	6467.0
38	6770.0
39	7098.0
40	8159.0
41	9187.0
42	10519.0
43	11851.0
44	19263.0
45	26675.0
46	23006.0
47	19337.0
48	19252.0
49	19153.0
50	17214.0
51	15279.0
52	15114.0
53	14959.0
54	14172.5
55	13386.0
56	12792.0
57	12198.0
58	11448.0
59	10698.0
60	10189.5
61	9663.0
62	8819.5
63	7985.0
64	6973.5
65	5962.5
66	4931.5
67	3909.0
68	3300.0
69	2691.0
70	2248.0
71	1805.0
72	1644.0
73	1478.5
74	1247.5
75	827.0
76	633.0
77	515.0
78	397.0
79	289.5
80	178.5
81	131.5
82	88.0
83	62.5
84	37.0
85	23.0
86	9.0
87	5.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	1.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
42	2323.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	255863.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.19389891470867
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.02365624283192	29.40469598993406
2	8.0448558374428	6.30618532952274
3	3.4218070330146637	4.023418768728478
4	2.2307848412863955	3.497326222793737
5	1.7628275316748876	3.454604204026547
6	1.445858473346845	3.400129850959874
7	1.2058819612458673	3.3084250984498813
8	1.0099011449323108	3.166557071066043
9	0.8819136730975523	3.1109071819396408
>10	4.908519525210407	29.356100312110872
>50	0.03399667220557216	0.928804487427113
>100	0.020997944597559277	1.5723053179567836
>500	0.002999706371079897	0.8182885104421148
>1k	0.004999510618466495	4.766687333576361
>5k	9.999021236932989E-4	2.8855643210657527
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7363	2.8518200057323013	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGC	3329	1.2893805241182714	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTC	3199	1.2390292269913938	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCG	2903	1.1243831966101958	No Hit
GCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	1456	0.5639345278210283	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAAACAGTT	1276	0.4942173471838132	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTA	754	0.2920375233358896	No Hit
GAACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCT	721	0.27925604021906686	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCT	613	0.23742573183673787	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTC	488	0.18901102306089407	No Hit
CCTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGC	369	0.1429202203062908	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCAAACAGTTCGTATGCCGTCTTCTGCT	320	0.12394165446616004	Illumina Single End Adapter 1 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	296	0.11464603038119806	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13633581991277607	0.0
2	0.0	0.0	0.0	1.0383986738242972	0.0
3	0.0	0.0	0.0	1.2022340483217526	0.0
4	0.0	0.0	0.0	1.7010992075480467	0.0
5	0.0	0.0	0.0	3.456035571254832	0.0
6	0.0	0.0	0.0	3.8387054294191008	0.0
7	0.0	0.0	0.0	4.425104382112121	0.0
8	0.0	0.0	0.0	5.05449559619809	0.0
9	0.0	0.0	0.0	5.228788547791127	0.0
10	0.0	0.0	0.0	6.828797843415212	0.0
11	0.0	0.0	0.0	7.48336470606462	0.0
12	0.0	0.0	0.0	9.435058446236434	0.0
13	0.0	0.0	0.0	9.745687217742248	0.0
14	0.0	0.0	0.0	9.901001603495155	0.0
15	0.0	0.0	0.0	10.402190668742689	0.0
16	0.0	0.0	0.0	10.81816984654474	0.0
17	0.0	0.0	0.0	11.234536342016996	0.0
18	0.0	0.0	0.0	11.648191613797804	0.0
19	0.0	0.0	0.0	12.452650414817224	0.0
20	0.0	0.0	0.0	12.791166058577925	0.0
21	0.0	0.0	0.0	13.121548031264282	0.0
22	0.0	0.0	0.0	13.463549534056844	0.0
23	0.0	0.0	0.0	13.798966636455889	0.0
24	0.0	0.0	0.0	14.051885075100897	0.0
25	0.0	0.0	0.0	14.278853229842053	0.0
26	0.0	0.0	0.0	14.504659431572588	0.0
27	0.0	0.0	0.0	14.772295941685451	0.0
28	0.0	0.0	0.0	15.002362637788261	0.0
29	0.0	0.0	0.0	15.238626416614379	0.0
30	0.0	0.0	0.0	15.49348144361042	0.0
31	0.0	0.0	0.0	15.755308188670183	0.0
32	0.0	0.0	0.0	16.020620792761807	0.0
33	0.0	0.0	0.0	16.278574361119503	0.0
34	0.0	0.0	0.0	16.53768988248782	0.0
35	0.0	0.0	0.0	16.802227851239028	0.0
36	0.0	0.0	0.0	17.075286808734788	0.0
37	0.0	0.0	0.0	17.352218942932616	0.0
38	0.0	0.0	0.0	17.653552090353465	0.0
39	0.0	0.0	0.0	17.95604719078494	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTCGAA	20	6.7793916E-4	45.323627	42
CGATCGA	20	6.7793916E-4	45.323627	41
CCGATCG	25	3.7214173E-5	45.323624	40
TAGGGCA	45	3.8926373E-10	44.91982	5
GCGTCCC	45	3.8926373E-10	44.91982	7
ATAGGGC	35	1.2240889E-7	44.919815	4
ATTCGCG	20	7.085089E-4	44.919815	1
TACGGGT	20	7.085089E-4	44.919815	4
ACGGGTA	30	2.1856104E-6	44.919815	5
GCCGATA	20	7.085089E-4	44.919815	32
CTAGTAG	20	7.085089E-4	44.919815	1
GCGATTA	20	7.085089E-4	44.919815	9
GCGATAT	20	7.085089E-4	44.919815	9
CGTAAGG	40	6.886694E-9	44.919815	2
CGCCGGT	20	7.085089E-4	44.919815	28
GCGTACG	20	7.085089E-4	44.919815	1
GCGTAAG	20	7.085089E-4	44.919815	1
CGGCGAA	100	0.0	44.91981	31
TACGGGA	25	3.923481E-5	44.91981	4
ACGGGTC	25	3.923481E-5	44.91981	5
>>END_MODULE