FastQCFastQC Report
Sat 18 Jun 2016
SRR3552042_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552042_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393696
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113742.889031130618548No Hit
GAATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC72951.8529525319027877No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCG32410.8232240104039665No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGC29810.7571832073478013No Hit
GAACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT26320.6685361293993335TruSeq Adapter, Index 15 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC18890.47981183451190773TruSeq Adapter, Index 21 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTT11990.30454970332439246No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTC8160.20726652036088758No Hit
GAATGACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCT7680.1950743721043648No Hit
GAATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTT5240.13309761846704057No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACCAGTATCGTA4720.11988945785580751No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT253.886116E-545.0000046
GCGCGAC253.886116E-545.0000049
GCGATTA302.1618507E-645.0000049
TTGCACG253.886116E-545.0000041
TCGACGG253.886116E-545.0000042
TAGCCCG207.027629E-445.01
CGTTTTT65000.044.030771
CGACGGG700.041.7857133
TGCACGG650.041.5384642
TACGGCT3600.041.257
ACGGCTG3700.040.1351368
GACACGA850.039.70588325
GCGGGCA800.039.3755
GAATCTG9450.039.2857171
AACACGT750.039.00000441
TAGGGCG1100.038.8636365
TGATACC4250.038.6470574
GATACCT4200.038.571435
AGGGCGA3150.038.571436
AGTTGCG356.2378276E-638.5714261
Produced by FastQC (version 0.11.5)