##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552042_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	393696
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.72153641388279	31.0	30.0	33.0	28.0	34.0
2	30.555784158335367	31.0	30.0	33.0	26.0	34.0
3	29.676168922214096	31.0	28.0	33.0	25.0	34.0
4	33.99639823620255	35.0	33.0	37.0	30.0	37.0
5	34.86719956514671	35.0	35.0	37.0	32.0	37.0
6	34.93870143460944	35.0	35.0	37.0	32.0	37.0
7	35.43714947573763	37.0	35.0	37.0	33.0	37.0
8	35.1599381248476	37.0	35.0	37.0	32.0	37.0
9	37.115589185564495	39.0	37.0	39.0	33.0	39.0
10	36.50062992765992	38.0	35.0	39.0	32.0	39.0
11	36.58382609932537	39.0	35.0	39.0	32.0	39.0
12	36.87351154190035	39.0	37.0	39.0	33.0	39.0
13	36.802106193611316	39.0	37.0	39.0	33.0	39.0
14	37.994262070226775	40.0	37.0	41.0	33.0	41.0
15	38.044148276843046	40.0	37.0	41.0	33.0	41.0
16	37.88046614646834	39.0	37.0	41.0	33.0	41.0
17	37.57818977078761	39.0	36.0	41.0	32.0	41.0
18	37.31893643826709	39.0	36.0	40.0	32.0	41.0
19	36.73341613834024	38.0	36.0	40.0	32.0	41.0
20	36.460672701780055	38.0	35.0	40.0	31.0	41.0
21	36.67787836300089	38.0	35.0	40.0	32.0	41.0
22	36.97471399252215	38.0	35.0	40.0	33.0	41.0
23	37.067252397789154	38.0	35.0	40.0	33.0	41.0
24	36.98082022677396	38.0	35.0	40.0	33.0	41.0
25	36.43696405348289	38.0	35.0	40.0	31.0	41.0
26	36.55035103226855	38.0	35.0	40.0	32.0	41.0
27	36.7800409452979	38.0	35.0	40.0	33.0	41.0
28	36.674401060716896	38.0	35.0	40.0	32.0	41.0
29	36.62203832398602	38.0	35.0	40.0	32.0	41.0
30	36.24352038120784	38.0	35.0	40.0	31.0	41.0
31	36.10945755100382	38.0	35.0	40.0	30.0	41.0
32	35.683326729253025	38.0	35.0	40.0	28.0	41.0
33	35.09506065593757	38.0	35.0	40.0	23.0	41.0
34	34.64467253921808	38.0	34.0	40.0	19.0	41.0
35	34.26349772413233	38.0	34.0	40.0	16.0	41.0
36	34.05657918800293	38.0	34.0	40.0	15.0	41.0
37	33.92061387466472	38.0	34.0	40.0	15.0	41.0
38	33.74493009835	38.0	33.0	40.0	12.0	41.0
39	33.620712427863126	38.0	33.0	40.0	12.0	41.0
40	33.410555352353086	38.0	33.0	40.0	10.0	41.0
41	33.21333465414939	38.0	33.0	40.0	10.0	41.0
42	33.206425770137365	38.0	33.0	40.0	10.0	41.0
43	33.165264569617165	38.0	33.0	40.0	10.0	41.0
44	33.124057648541005	38.0	33.0	40.0	10.0	41.0
45	33.09521559782167	38.0	33.0	40.0	10.0	41.0
46	32.88322970007315	37.0	32.0	40.0	10.0	41.0
47	32.77243863285378	37.0	32.0	40.0	10.0	41.0
48	32.73822441680891	37.0	32.0	40.0	10.0	41.0
49	32.67690299114037	37.0	32.0	40.0	10.0	41.0
50	32.59165193448752	37.0	31.0	40.0	10.0	41.0
51	30.874161789807363	35.0	27.0	39.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	9.0
9	24.0
10	33.0
11	15.0
12	19.0
13	18.0
14	28.0
15	36.0
16	71.0
17	157.0
18	334.0
19	562.0
20	897.0
21	1379.0
22	1942.0
23	2964.0
24	4712.0
25	7904.0
26	11636.0
27	13128.0
28	12344.0
29	10982.0
30	10148.0
31	10937.0
32	12808.0
33	17178.0
34	25702.0
35	29268.0
36	33902.0
37	49083.0
38	77849.0
39	57625.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.380628708445094	14.160672193773877	17.797996423636512	29.660702674144517
2	40.59934568804356	24.78816142404292	20.237696090384457	14.374796797529058
3	26.619777696496786	23.40841664634642	36.861182231975945	13.11062342518085
4	23.498587742826953	19.987757051125744	40.780703893359345	15.732951312687963
5	23.054336340729904	23.345677883443063	36.206362269365194	17.39362350646184
6	21.308573112249046	29.768399983743805	36.450713240673004	12.472313663334146
7	65.53686092822889	4.478328456474031	26.218960822563602	3.76584979273348
8	65.77613183776315	6.005649028692188	23.7165223929123	4.501696740632366
9	60.160631553279686	6.297752580671381	24.870458424774444	8.671157441274486
10	32.09049622043404	24.909828903519468	30.564699666747945	12.434975209298544
11	24.13740551085101	21.079716329350564	38.798717792408354	15.984160367390068
12	22.061184264000648	18.524953263431684	41.99026660164187	17.42359587092579
13	19.871931642688775	19.963626757701373	44.12541656506543	16.039025034544423
14	17.888167520117047	23.75716288710071	40.357026741445175	17.997642851337073
15	17.134032349833376	22.339063643013898	44.335223116313095	16.19168089083963
16	20.183085426318783	23.220200357636347	38.94248354059985	17.654230675445014
17	20.040081687393318	22.17675566934894	39.48452613183776	18.29863651141998
18	19.90495204421686	22.404850442981385	39.58511135495408	18.10508615784768
19	20.125934731366332	23.265920913598308	37.820043891733725	18.78810046330163
20	21.478755181663008	23.41781476062749	39.67223441437048	15.431195643339024
21	21.454116882061285	23.61644517597334	39.107839551328944	15.82159839063643
22	20.332947248638543	20.335487279525317	39.59781150938795	19.733753962448183
23	19.962102739169307	22.787125091441112	40.324514346094446	16.92625782329513
24	21.872205966024545	21.032471754856537	38.363102495326345	18.73221978379257
25	19.571699991871903	25.116587417703002	36.960751442737546	18.350961147687556
26	19.30880679509063	22.162531496382996	38.943245549865885	19.58541615866049
27	20.504907339673252	22.91946070064212	37.4400552710721	19.13557668861253
28	17.37381126554499	23.232138502804194	39.81269812240917	19.58135210924165
29	19.660601072909046	22.384276192798506	39.30367593269934	18.651446801593107
30	19.147768836869055	24.55600260099163	38.43320734780135	17.863021214337966
31	20.93087051938552	21.81353125254003	36.03440217833049	21.221196049743966
32	20.690837600585223	23.54049825245875	36.180453954320086	19.588210192635945
33	19.9882650573031	26.07798910834756	34.242410387710315	19.69133544663903
34	21.57756238315858	24.564892709095343	36.14616353734861	17.711381370397465
35	19.24098797041372	26.441213525156464	34.593188653174025	19.724609851255792
36	19.536139559457045	29.096815817280337	32.781384621637	18.58566000162562
37	20.534625701048526	27.334034381858086	32.900512070226775	19.230827846866617
38	20.301704868731203	27.641378119157928	30.892363651141995	21.164553360968867
39	23.160255628708445	25.37135251564659	31.672661139559455	19.795730716085508
40	21.10943469072584	26.23267698935219	34.46415508412583	18.19373323579615
41	19.84018125660408	28.493558481671137	32.17279322116557	19.493467040559214
42	20.360633585304395	26.046492725351538	33.940400715272695	19.652472974071365
43	22.20469600910347	25.848624319271725	31.37268349183126	20.573996179793546
44	21.100798585710802	25.128525562870845	31.921838169552142	21.84883768186621
45	20.654007152726976	23.959349345688043	31.927680240591727	23.458963260993254
46	22.467589205884746	25.090171096480535	31.998547102332765	20.44369259530196
47	19.777696496789403	24.182618060635615	35.99401568723076	20.045669755344225
48	20.35809355441762	24.761999105909126	33.11514468015931	21.764762659513938
49	19.79852475006096	24.156709745590508	35.815959522067786	20.228805982280747
50	19.7380720149557	23.9179468422336	34.977749329431845	21.36623181337885
51	19.45435056490287	23.54405429570024	33.40013614565553	23.601458993741364
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	124.0
1	446.5
2	769.0
3	10809.0
4	20849.0
5	14252.5
6	7656.0
7	7131.0
8	6606.0
9	6353.0
10	6100.0
11	5841.0
12	5582.0
13	5253.5
14	4925.0
15	4710.0
16	4495.0
17	4087.0
18	3679.0
19	3464.5
20	3250.0
21	3118.5
22	2987.0
23	2864.0
24	2741.0
25	2914.0
26	3262.0
27	3437.0
28	4022.5
29	4608.0
30	5380.5
31	6153.0
32	6788.5
33	7424.0
34	8429.5
35	9435.0
36	10110.0
37	10785.0
38	11811.5
39	12838.0
40	13915.5
41	14993.0
42	16692.0
43	18391.0
44	29990.5
45	41590.0
46	33408.0
47	25226.0
48	25952.5
49	26679.0
50	24348.5
51	22018.0
52	20783.0
53	19548.0
54	18589.0
55	17630.0
56	16764.5
57	15899.0
58	15190.5
59	14482.0
60	14107.5
61	13733.0
62	12450.5
63	11168.0
64	9817.0
65	8466.0
66	6971.0
67	5476.0
68	4663.5
69	3851.0
70	3300.5
71	2750.0
72	2229.5
73	1709.0
74	1369.5
75	849.0
76	668.0
77	540.0
78	412.0
79	304.0
80	196.0
81	152.0
82	108.0
83	87.5
84	67.0
85	50.5
86	34.0
87	27.5
88	21.0
89	15.0
90	9.0
91	7.0
92	5.0
93	5.0
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	393696.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.0217350438117
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.93226140020252	32.38515213862122
2	9.654459947682358	8.693210755112949
3	5.184945852632601	7.003057751812058
4	3.511764422771716	6.324229095132503
5	2.5648638371466954	5.773731004973637
6	1.9076022421995285	5.153013763037298
7	1.4033663110458499	4.4227390357722385
8	1.021196697471526	3.6780837712942875
9	0.7246564907742532	2.936276327287513
>10	2.0382974194755406	12.814133566936786
>50	0.03429416884690267	1.0738401649359164
>100	0.016575514942669584	1.3229360862534236
>500	0.0017147084423451294	0.5424526881583771
>1k	0.002857847403908549	3.0730407979067076
>5k	5.715694807817098E-4	1.8772259772843267
>10k+	5.715694807817098E-4	2.926877075480731
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11374	2.889031130618548	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC	7295	1.8529525319027877	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCG	3241	0.8232240104039665	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGC	2981	0.7571832073478013	No Hit
GAACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT	2632	0.6685361293993335	TruSeq Adapter, Index 15 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	1889	0.47981183451190773	TruSeq Adapter, Index 21 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTT	1199	0.30454970332439246	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTC	816	0.20726652036088758	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCT	768	0.1950743721043648	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTT	524	0.13309761846704057	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACCAGTATCGTA	472	0.11988945785580751	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5400308867755834E-4	0.0	0.0	0.07721693895797772	0.0
2	2.5400308867755834E-4	0.0	0.0	0.7795354791514265	0.0
3	2.5400308867755834E-4	0.0	0.0	0.9753718605218239	0.0
4	2.5400308867755834E-4	0.0	0.0	1.8610806307404697	0.0
5	2.5400308867755834E-4	0.0	0.0	4.322624563114688	0.0
6	2.5400308867755834E-4	0.0	0.0	5.123496301715029	0.0
7	2.5400308867755834E-4	0.0	0.0	5.671888970169877	0.0
8	2.5400308867755834E-4	0.0	0.0	6.371413476387873	0.0
9	2.5400308867755834E-4	0.0	0.0	6.682567260017882	0.0
10	2.5400308867755834E-4	0.0	0.0	7.974680972120621	0.0
11	2.5400308867755834E-4	0.0	0.0	8.433918556449646	0.0
12	2.5400308867755834E-4	0.0	0.0	9.637639193692594	0.0
13	2.5400308867755834E-4	0.0	0.0	9.895960334877673	0.0
14	2.5400308867755834E-4	0.0	0.0	10.077064537104771	0.0
15	2.5400308867755834E-4	0.0	0.0	10.343767780216208	0.0
16	2.5400308867755834E-4	0.0	0.0	10.575672600178818	0.0
17	2.5400308867755834E-4	0.0	0.0	10.81672153133382	0.0
18	2.5400308867755834E-4	0.0	0.0	11.071232626188735	0.0
19	2.5400308867755834E-4	0.0	0.0	11.283833211411851	0.0
20	2.5400308867755834E-4	0.0	0.0	11.461127367308787	0.0
21	2.5400308867755834E-4	0.0	0.0	11.653661708526375	0.0
22	2.5400308867755834E-4	0.0	0.0	11.882264488336178	0.0
23	2.5400308867755834E-4	0.0	0.0	12.110105258879948	0.0
24	2.5400308867755834E-4	0.0	0.0	12.279017312850524	0.0
25	2.5400308867755834E-4	0.0	0.0	12.457073478013493	0.0
26	2.5400308867755834E-4	0.0	0.0	12.6290335690482	0.0
27	2.5400308867755834E-4	0.0	0.0	12.832236039990246	0.0
28	2.5400308867755834E-4	0.0	0.0	13.017912297813542	0.0
29	2.5400308867755834E-4	0.0	0.0	13.238386978785663	0.0
30	2.5400308867755834E-4	0.0	0.0	13.468005770950175	0.0
31	2.5400308867755834E-4	2.5400308867755834E-4	0.0	13.685432414858164	0.0
32	2.5400308867755834E-4	2.5400308867755834E-4	0.0	13.92825936763391	0.0
33	2.5400308867755834E-4	2.5400308867755834E-4	0.0	14.154830122734293	0.0
34	2.5400308867755834E-4	2.5400308867755834E-4	0.0	14.362858652361213	0.0
35	2.5400308867755834E-4	2.5400308867755834E-4	0.0	14.57952328700317	0.0
36	2.5400308867755834E-4	2.5400308867755834E-4	0.0	14.795679915467773	0.0
37	2.5400308867755834E-4	2.5400308867755834E-4	0.0	15.026568723075673	0.0
38	2.5400308867755834E-4	2.5400308867755834E-4	0.0	15.316386247256766	0.0
39	2.5400308867755834E-4	2.5400308867755834E-4	0.0	15.625254003088678	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	25	3.886116E-5	45.000004	6
GCGCGAC	25	3.886116E-5	45.000004	9
GCGATTA	30	2.1618507E-6	45.000004	9
TTGCACG	25	3.886116E-5	45.000004	1
TCGACGG	25	3.886116E-5	45.000004	2
TAGCCCG	20	7.027629E-4	45.0	1
CGTTTTT	6500	0.0	44.03077	1
CGACGGG	70	0.0	41.785713	3
TGCACGG	65	0.0	41.538464	2
TACGGCT	360	0.0	41.25	7
ACGGCTG	370	0.0	40.135136	8
GACACGA	85	0.0	39.705883	25
GCGGGCA	80	0.0	39.375	5
GAATCTG	945	0.0	39.285717	1
AACACGT	75	0.0	39.000004	41
TAGGGCG	110	0.0	38.863636	5
TGATACC	425	0.0	38.647057	4
GATACCT	420	0.0	38.57143	5
AGGGCGA	315	0.0	38.57143	6
AGTTGCG	35	6.2378276E-6	38.571426	1
>>END_MODULE