FastQCFastQC Report
Sat 18 Jun 2016
SRR3552041_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552041_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390029
Sequences flagged as poor quality0
Sequence length42-51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104642.682877427063116No Hit
GAATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC62121.5927020811273007No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCG27640.7086652530965646No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGC26200.6717449215314758No Hit
GAACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT22070.5658553594732699TruSeq Adapter, Index 15 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC15970.40945673270449123TruSeq Adapter, Index 21 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACCAGTAT9070.23254681062177426No Hit
GAATGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTT8370.21459942722207836No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTC6840.17537157493417158No Hit
GAATGACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCT6670.17101292467995968No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACCAGTATCGTA5180.1328106371577498No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGG207.092708E-444.9157682
CGCGCAA207.092708E-444.91576818
CGACAGG207.092708E-444.9157682
TATTGCG207.092708E-444.9157681
AGTGCGA207.092708E-444.91576811
GGACGTA253.9292812E-544.9157688
AACGACG207.092708E-444.91576813
CGTTTTT64050.043.9690671
CGATGAA5250.042.7769219
ATCCGGC700.041.7074976
CCGATGA5300.041.52589818
TCGGGAT603.6379788E-1241.1727875
ACCACCG6450.040.7375614
GCGAACC1050.040.6380833
ATGGGCG950.040.1877945
ACACGAC850.039.6315626
TGTACGG403.5014637E-739.3012962
GTTTTTT73650.039.000182
CCACCGA6400.038.95039415
GATGAAT6300.038.85570520
Produced by FastQC (version 0.11.5)