##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552041_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	390029
Sequences flagged as poor quality	0
Sequence length	42-51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.116970789351562	30.0	28.0	33.0	25.0	33.0
2	28.40101889859472	31.0	28.0	33.0	16.0	33.0
3	27.99660794453746	31.0	25.0	33.0	16.0	34.0
4	32.876891205525745	35.0	32.0	35.0	28.0	37.0
5	34.125852180222495	35.0	33.0	35.0	32.0	37.0
6	34.09626720064406	35.0	35.0	37.0	30.0	37.0
7	34.70265287965767	35.0	35.0	37.0	32.0	37.0
8	34.27445138694815	35.0	35.0	37.0	31.0	37.0
9	36.267087831930446	39.0	35.0	39.0	31.0	39.0
10	35.87744501049922	37.0	35.0	39.0	31.0	39.0
11	36.31129480115581	37.0	35.0	39.0	32.0	39.0
12	36.49046096572306	38.0	35.0	39.0	32.0	39.0
13	36.54444925890126	39.0	35.0	39.0	32.0	39.0
14	37.47953357314456	39.0	36.0	40.0	32.0	41.0
15	37.676893256655276	39.0	37.0	41.0	33.0	41.0
16	37.480764250863395	39.0	36.0	41.0	32.0	41.0
17	37.35725035830669	39.0	36.0	40.0	32.0	41.0
18	37.04901430406457	39.0	36.0	40.0	32.0	41.0
19	36.54495434954837	37.0	35.0	40.0	31.0	41.0
20	36.348809960285	38.0	35.0	40.0	31.0	41.0
21	36.5539075299529	38.0	35.0	40.0	32.0	41.0
22	36.77182209528009	38.0	35.0	40.0	32.0	41.0
23	36.96344118001482	38.0	35.0	40.0	32.0	41.0
24	36.89557699555674	38.0	35.0	40.0	32.0	41.0
25	36.28129703175917	38.0	35.0	40.0	31.0	41.0
26	36.37462598934951	38.0	35.0	40.0	31.0	41.0
27	36.55958915875486	38.0	35.0	40.0	32.0	41.0
28	36.4762286906871	38.0	35.0	40.0	31.0	41.0
29	36.48629973668625	38.0	35.0	40.0	31.0	41.0
30	36.032584756518105	38.0	35.0	40.0	30.0	41.0
31	35.933910042586575	38.0	35.0	40.0	30.0	41.0
32	35.362778152393794	38.0	35.0	40.0	25.0	41.0
33	34.93115127336685	38.0	35.0	40.0	22.0	41.0
34	34.50973901940625	38.0	34.0	40.0	18.0	41.0
35	34.12173966551205	38.0	34.0	40.0	15.0	41.0
36	33.99805399085709	38.0	34.0	40.0	15.0	41.0
37	33.920005948275644	38.0	34.0	40.0	12.0	41.0
38	33.78366993223581	38.0	34.0	40.0	12.0	41.0
39	33.699471064972094	38.0	33.0	40.0	12.0	41.0
40	33.52754282373875	38.0	33.0	40.0	11.0	41.0
41	33.40346743447282	38.0	33.0	40.0	12.0	41.0
42	33.44852818636563	38.0	33.0	40.0	10.0	41.0
43	33.43910467923884	38.0	33.0	40.0	10.0	41.0
44	33.46282048295976	38.0	33.0	40.0	11.0	41.0
45	33.4289729276492	38.0	33.0	40.0	11.0	41.0
46	33.27956388179364	38.0	33.0	40.0	10.0	41.0
47	33.24546788506479	37.0	33.0	40.0	11.0	41.0
48	33.16105732526934	37.0	33.0	40.0	10.0	41.0
49	33.17840681764659	37.0	33.0	40.0	10.0	41.0
50	33.10523046823444	37.0	33.0	40.0	10.0	41.0
51	32.23192782776799	36.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	5.0
10	6.0
11	9.0
12	12.0
13	12.0
14	12.0
15	54.0
16	80.0
17	162.0
18	359.0
19	654.0
20	1204.0
21	1917.0
22	2684.0
23	3689.0
24	5419.0
25	8106.0
26	11326.0
27	12353.0
28	11213.0
29	9787.0
30	9666.0
31	10775.0
32	13428.0
33	18904.0
34	26141.0
35	31626.0
36	39011.0
37	58197.0
38	79015.0
39	34201.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.960949057634174	14.236890077404501	17.77508851905884	29.027072345902482
2	40.803888941591524	24.277169133577246	20.038253565760495	14.880688359070735
3	26.656222998802654	23.23596450520346	36.2234603067977	13.88435218919619
4	23.427232334005932	20.17824315627812	40.40058559748122	15.993938912234732
5	24.84020418994485	23.16802083947604	35.028420963569374	16.963354007009734
6	21.5717292816688	30.635414289706663	34.97124572788177	12.821610700742767
7	64.95234969707381	4.524278963871917	26.714167408064526	3.809203930989747
8	65.52102535965274	7.284073748362302	22.44089542059693	4.754005471388025
9	60.050919290616854	6.2382540785428775	23.561324927120804	10.149501703719467
10	33.83645831463814	24.754569532009157	29.320127477700375	12.088844675652323
11	26.039858574618812	20.692307495083696	37.46849593235375	15.799337997943743
12	21.936573947065476	18.545544049288644	40.26213435411213	19.255747649533753
13	22.046309376995044	19.5631606880514	42.221219447784655	16.169310487168904
14	17.990200728663766	24.93660727792036	39.1940086506388	17.879183342777075
15	17.3069182035182	21.846580638875572	42.63939348099757	18.207107676608665
16	20.144399519010125	22.74061672337185	37.95435724010266	19.160626517515364
17	20.00158962538683	23.537224155126925	38.39945234841512	18.061733871071127
18	20.07978893877122	21.80709639539624	38.430219291386024	19.682895374446517
19	20.19926723397491	22.823943860584723	36.874950324206665	20.101838581233704
20	22.992392873350443	22.814713777693456	38.73660676513798	15.456286583818127
21	21.516348784321167	24.478436218845264	37.99691817787908	16.008296818954488
22	20.442069692253654	20.137733348033095	40.219573416335706	19.200623543377542
23	21.482761538244592	22.342441203090026	39.061710795863895	17.113086462801483
24	21.391229882906142	22.461919498293717	37.64284194252222	18.504008676277916
25	19.491114763261194	25.667578564670833	36.41139505011166	18.429911621956315
26	19.2393386132826	22.089383097154315	39.452707362785844	19.21857092677724
27	21.71120608980358	22.62652264318807	36.871617238718144	18.790654028290206
28	17.59510190267903	24.765081570857554	38.549441195398295	19.090375331065125
29	19.77596537693351	22.06041089252338	39.61961802840301	18.544005702140097
30	19.310358973307114	25.480669386122575	37.223129562160764	17.985842078409554
31	20.713331572780486	21.801968571567755	35.02508787808086	22.459611977570898
32	21.857092677723962	23.803614603016698	35.25225047368273	19.087042245576612
33	21.483530711818865	25.696294378110345	33.0529268336457	19.767248076425087
34	21.562242807586102	24.804822205528307	34.56204538636871	19.070889600516885
35	20.430019306256714	26.217024887892954	33.576477646533974	19.77647815931636
36	19.859292514146386	29.38166136364219	31.76635583507893	18.992690287132497
37	20.930494911916806	27.214899404916043	31.941214627630256	19.9133910555369
38	20.412584705239865	27.18541441790226	30.487989354637733	21.914011522220143
39	22.737027246691913	25.732958318484012	30.905906996659223	20.624107438164856
40	20.995874665729986	25.987554771568266	33.5090467631894	19.507523799512345
41	20.089788195236764	27.62563809357766	31.486633045235095	20.79794066595048
42	20.648464601350156	25.952429178343152	32.99293129485243	20.406174925454263
43	21.685830298983717	25.936248896894227	31.172669373642954	21.205251430479105
44	21.466891299115705	25.135284116053196	31.65557738044404	21.74224720438706
45	20.897805175345294	23.797064768160162	31.60485392420984	23.700276132284703
46	22.435295244158393	25.045482895067146	31.670328589655	20.84889327111946
47	19.87738380834815	25.345682942167496	34.726675620909454	20.050257628574904
48	20.698793247569284	24.858116658773476	32.50907716952762	21.934012924129615
49	20.40558061535834	23.87444391823172	35.17024706981463	20.549728396595317
50	19.879195360356512	24.495547463956584	33.890773765621404	21.7344834100655
51	19.51507340667531	23.7952532161518	33.24715521636401	23.442518160808884
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	108.0
1	412.0
2	716.0
3	9534.0
4	18479.5
5	12838.5
6	6973.5
7	6511.5
8	6140.5
9	6070.0
10	6005.0
11	5746.0
12	5487.0
13	5252.5
14	5018.0
15	4758.0
16	4498.0
17	4092.0
18	3676.5
19	3472.5
20	3283.5
21	3041.5
22	2794.0
23	2723.5
24	2653.0
25	2839.0
26	3258.5
27	3494.0
28	3944.0
29	4392.0
30	5162.5
31	5933.0
32	6480.0
33	7027.0
34	8133.5
35	9254.0
36	9841.5
37	10438.5
38	11456.0
39	12489.5
40	13592.0
41	14655.0
42	15821.0
43	16987.0
44	27370.0
45	37753.0
46	31774.0
47	25795.0
48	26091.0
49	26363.5
50	24543.0
51	22743.5
52	21730.5
53	20720.0
54	19809.5
55	18899.0
56	17929.0
57	16959.0
58	16023.5
59	15088.0
60	14643.5
61	14175.5
62	12897.5
63	11621.0
64	10241.0
65	8864.0
66	7374.0
67	5903.0
68	5002.5
69	4102.0
70	3580.0
71	3058.0
72	2528.0
73	1994.5
74	1687.5
75	1121.5
76	859.0
77	648.0
78	437.0
79	353.0
80	267.5
81	189.0
82	111.5
83	88.0
84	65.0
85	37.0
86	9.0
87	6.5
88	4.0
89	3.0
90	2.0
91	2.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
42	3620.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	386409.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.132756936334346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.99940809830426	34.40663358393652
2	9.571615183182084	9.022732238340971
3	5.154012626871989	7.287684731674666
4	3.34356266035035	6.30365304686786
5	2.400714893907576	5.6576155783991755
6	1.7558042984304782	4.965353833541688
7	1.2715336239548751	4.195161966396905
8	1.0016471323486027	3.776831265997041
9	0.6891521630096267	2.9233477252153626
>10	1.7656171240840335	11.703010378307631
>50	0.022637882562359667	0.7062925623450124
>100	0.01822073474531373	1.3805755501990664
>500	0.002760717385653595	0.940248720616254
>1k	0.002208573908522876	2.3910891904295988
>5k	5.52143477130719E-4	1.616613632014439
>10k+	5.52143477130719E-4	2.7231559957178195
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10464	2.682877427063116	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTC	6212	1.5927020811273007	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCG	2764	0.7086652530965646	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGC	2620	0.6717449215314758	No Hit
GAACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCT	2207	0.5658553594732699	TruSeq Adapter, Index 15 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTTCTGC	1597	0.40945673270449123	TruSeq Adapter, Index 21 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACCAGTAT	907	0.23254681062177426	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCTT	837	0.21459942722207836	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTC	684	0.17537157493417158	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTACCAGTATCGTATGCCGTCT	667	0.17101292467995968	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACCAGTATCGTA	518	0.1328106371577498	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06204666832466304	0.0
2	0.0	0.0	0.0	0.6091854708239643	0.0
3	0.0	0.0	0.0	0.7835314809924391	0.0
4	0.0	0.0	0.0	1.532193759951183	0.0
5	0.0	0.0	0.0	3.4876893769437656	0.0
6	0.0	0.0	0.0	4.140974132692698	0.0
7	0.0	0.0	0.0	4.662217424858151	0.0
8	0.0	0.0	0.0	5.290888626230357	0.0
9	0.0	0.0	0.0	5.591379102579552	0.0
10	0.0	0.0	0.0	6.722320647951819	0.0
11	0.0	0.0	0.0	7.176389447964126	0.0
12	0.0	0.0	0.0	8.255796363860123	0.0
13	0.0	0.0	0.0	8.5042394283502	0.0
14	0.0	0.0	0.0	8.667560617287432	0.0
15	0.0	0.0	0.0	8.933181891602931	0.0
16	0.0	0.0	0.0	9.1593189224391	0.0
17	0.0	0.0	0.0	9.424427414371753	0.0
18	0.0	0.0	0.0	9.641847144699497	0.0
19	0.0	0.0	0.0	10.05386778931823	0.0
20	0.0	0.0	0.0	10.269492781306006	0.0
21	0.0	0.0	0.0	10.454607221514298	0.0
22	0.0	0.0	0.0	10.686128467370375	0.0
23	0.0	0.0	0.0	10.898933156252484	0.0
24	0.0	0.0	0.0	11.078406990249443	0.0
25	0.0	0.0	0.0	11.258649997820674	0.0
26	0.0	0.0	0.0	11.43914939658333	0.0
27	0.0	0.0	0.0	11.625802183940168	0.0
28	0.0	0.0	0.0	11.806301582702824	0.0
29	0.0	0.0	0.0	11.99705662912245	0.0
30	0.0	0.0	0.0	12.211912447535953	0.0
31	0.0	2.5639119142422745E-4	0.0	12.437023913606424	0.0
32	0.0	2.5639119142422745E-4	0.0	12.648546646531411	0.0
33	0.0	2.5639119142422745E-4	0.0	12.865453594476309	0.0
34	0.0	2.5639119142422745E-4	0.0	13.064669550212933	0.0
35	0.0	2.5639119142422745E-4	0.0	13.277730630286467	0.0
36	0.0	2.5639119142422745E-4	0.0	13.487971407254332	0.0
37	0.0	2.5639119142422745E-4	0.0	13.720261826684682	0.0
38	0.0	2.5639119142422745E-4	0.0	13.991010924828666	0.0
39	0.0	2.5639119142422745E-4	0.0	14.282014927095165	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACGG	20	7.092708E-4	44.915768	2
CGCGCAA	20	7.092708E-4	44.915768	18
CGACAGG	20	7.092708E-4	44.915768	2
TATTGCG	20	7.092708E-4	44.915768	1
AGTGCGA	20	7.092708E-4	44.915768	11
GGACGTA	25	3.9292812E-5	44.915768	8
AACGACG	20	7.092708E-4	44.915768	13
CGTTTTT	6405	0.0	43.969067	1
CGATGAA	525	0.0	42.77692	19
ATCCGGC	70	0.0	41.707497	6
CCGATGA	530	0.0	41.525898	18
TCGGGAT	60	3.6379788E-12	41.172787	5
ACCACCG	645	0.0	40.73756	14
GCGAACC	105	0.0	40.63808	33
ATGGGCG	95	0.0	40.187794	5
ACACGAC	85	0.0	39.63156	26
TGTACGG	40	3.5014637E-7	39.301296	2
GTTTTTT	7365	0.0	39.00018	2
CCACCGA	640	0.0	38.950394	15
GATGAAT	630	0.0	38.855705	20
>>END_MODULE