FastQCFastQC Report
Sat 18 Jun 2016
SRR3552040_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552040_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245663
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58862.39596520436533No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTC43461.7690901763798375No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCG43151.7564712634788309No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGC40031.6294680110557962No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC14360.5845406105111475No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT8730.355364869760607No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCT8310.3382682780882754No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTA7130.2902349967231532No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTC6970.2837220094194079No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTT4590.18684132327619543No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTT2790.11357021610905998No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCTA253.881823E-545.00000445
AGCTACG253.881823E-545.0000049
GTTGATC351.2067176E-745.016
GGTACAC207.022448E-445.08
CGAAAAA351.2067176E-745.022
CGATTTA207.022448E-445.010
ATGTCGA207.022448E-445.011
CATACGA351.2067176E-745.018
TAAGGCG207.022448E-445.01
AATAGCG207.022448E-445.01
TAAGCCT207.022448E-445.045
CGTATGG302.158511E-644.9999962
CGTTTTT33600.043.6607131
TACGGCT5050.042.7722747
TGATACC5600.041.7857134
GATACCT5600.041.7857135
GAATCTG5900.041.56781
ATACGGC9100.041.0439576
GCGACTG1100.040.90909211
TGATACG9150.040.819674