FastQCFastQC Report
Sat 18 Jun 2016
SRR3552039_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552039_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247250
Sequences flagged as poor quality0
Sequence length42-51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53912.1803842264914057No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTC39171.58422649140546No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGC36871.4912032355915066No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCG35971.4548028311425683No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTACAGT14330.5795753286147624No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC13330.5391304347826087No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTA8640.34944388270980786No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT8210.33205257836198177No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCT6830.27623862487360973No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTC6040.2442871587462083No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC2490.10070778564206267No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGG207.088668E-444.914462
GCGAACC406.892151E-944.9144633
CGTTGAT453.8926373E-1044.9144625
ACGGGTC302.1870273E-644.914465
ACGGGCG302.1870273E-644.914465
AACGGGC351.2249438E-744.914464
TGACCCA302.1870273E-644.9144611
ACAGCGG351.2249438E-744.914462
GCGATTA207.088668E-444.914469
GCGATAC253.9257582E-544.9144633
GGCATTA207.088668E-444.914467
ATAACGC207.088668E-444.9144611
GCTACGA302.1870273E-644.9144610
GTAAGCG207.088668E-444.914461
TAAGCGG253.9257582E-544.914462
GGCCTAT453.8926373E-1044.914468
GCCAATC207.088668E-444.9144615
AATCGTT453.8926373E-1044.9144622
AGTAACG207.088668E-444.914461
TAATCGT453.8926373E-1044.9144621
Produced by FastQC (version 0.11.5)