##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552039_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	247250
Sequences flagged as poor quality	0
Sequence length	42-51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.0684812942366	30.0	28.0	33.0	25.0	33.0
2	28.181023255813955	31.0	26.0	33.0	16.0	33.0
3	27.804242669362992	31.0	25.0	33.0	16.0	34.0
4	32.96790697674419	35.0	32.0	35.0	28.0	37.0
5	34.160986855409504	35.0	33.0	35.0	32.0	37.0
6	34.12438422649141	35.0	35.0	37.0	30.0	37.0
7	34.67588270980789	35.0	35.0	37.0	32.0	37.0
8	33.719906976744184	35.0	35.0	37.0	28.0	37.0
9	35.8730111223458	39.0	35.0	39.0	30.0	39.0
10	35.624922143579376	37.0	34.0	39.0	31.0	39.0
11	36.17666734074823	37.0	35.0	39.0	32.0	39.0
12	36.29618604651163	37.0	35.0	39.0	32.0	39.0
13	36.28336097067745	38.0	35.0	39.0	32.0	39.0
14	37.150285136501516	39.0	36.0	40.0	32.0	41.0
15	37.36567037411527	39.0	36.0	40.0	32.0	41.0
16	37.19015571284125	39.0	36.0	40.0	32.0	41.0
17	37.09917492416582	38.0	35.0	40.0	32.0	41.0
18	36.85407077856421	39.0	36.0	40.0	32.0	41.0
19	36.38902325581395	37.0	35.0	40.0	31.0	41.0
20	36.14452173913043	37.0	35.0	40.0	31.0	41.0
21	36.494058645096054	38.0	35.0	40.0	31.0	41.0
22	36.68157330637007	38.0	35.0	40.0	32.0	41.0
23	36.82220829120324	38.0	35.0	40.0	32.0	41.0
24	36.79370677451972	38.0	35.0	40.0	32.0	41.0
25	36.27648533872598	38.0	35.0	40.0	31.0	41.0
26	36.33299494438827	38.0	35.0	40.0	31.0	41.0
27	36.404424671385236	38.0	35.0	40.0	31.0	41.0
28	36.336537917087966	38.0	35.0	40.0	31.0	41.0
29	36.39938523761375	38.0	35.0	40.0	31.0	41.0
30	35.94494236602629	38.0	35.0	40.0	30.0	41.0
31	35.875789686552075	38.0	35.0	40.0	30.0	41.0
32	35.3269160768453	38.0	35.0	40.0	25.0	41.0
33	34.98144792719919	38.0	35.0	40.0	22.0	41.0
34	34.69239635995955	38.0	35.0	40.0	21.0	41.0
35	34.35276036400404	38.0	34.0	40.0	18.0	41.0
36	34.20894236602629	38.0	34.0	40.0	16.0	41.0
37	34.075987866531854	38.0	34.0	40.0	15.0	41.0
38	33.96025075834176	38.0	34.0	40.0	15.0	41.0
39	33.89908998988878	38.0	34.0	40.0	14.0	41.0
40	33.768550050556115	38.0	33.0	40.0	13.0	41.0
41	33.71149848331648	38.0	33.0	40.0	15.0	41.0
42	33.66870778564206	38.0	33.0	40.0	13.0	41.0
43	33.634599838318515	38.0	33.0	40.0	12.0	41.0
44	33.75641622367572	38.0	34.0	40.0	15.0	41.0
45	33.760127548729	38.0	34.0	40.0	15.0	41.0
46	33.61254419702277	38.0	33.0	40.0	15.0	41.0
47	33.54686721703698	37.0	33.0	40.0	15.0	41.0
48	33.46761879098177	37.0	33.0	40.0	15.0	41.0
49	33.527824730735	37.0	33.0	40.0	15.0	41.0
50	33.39251447375942	37.0	33.0	40.0	13.0	41.0
51	32.5244645321444	36.0	31.0	40.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	2.0
10	2.0
11	4.0
12	10.0
13	14.0
14	24.0
15	19.0
16	39.0
17	127.0
18	243.0
19	611.0
20	1062.0
21	1547.0
22	1858.0
23	2349.0
24	3171.0
25	4659.0
26	6356.0
27	7009.0
28	6445.0
29	5878.0
30	5892.0
31	6970.0
32	8994.0
33	13113.0
34	19072.0
35	20689.0
36	24906.0
37	36977.0
38	48971.0
39	20235.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.92335692618806	14.432760364004043	17.22143579373104	25.422446916076847
2	37.04388270980788	27.877856420626895	20.248331648129422	14.829929221435794
3	25.69908998988878	26.58685540950455	32.90839231547017	14.805662285136501
4	24.179575328614764	20.21880687563195	40.4469160768453	15.154701718907987
5	28.412537917087967	22.625278058645097	31.778361981799797	17.18382204246714
6	20.706572295247724	34.659251769464106	32.17189079878665	12.462285136501517
7	62.690394337714864	5.354095045500506	27.633569261880687	4.321941354903943
8	62.55288169868554	11.710414560161778	20.379777553083922	5.356926188068757
9	58.25642062689585	6.444084934277047	20.77330637007078	14.52618806875632
10	38.5670374115268	19.36865520728008	28.19737108190091	13.866936299292215
11	31.372295247724974	20.25237613751264	32.306572295247726	16.068756319514662
12	23.74519716885743	18.476440849342772	36.37775530839232	21.40060667340748
13	24.31506572295248	20.324772497472193	39.261071789686554	16.099089989888775
14	19.26835187057634	27.00303336703741	35.02891809908999	18.69969666329626
15	17.28210313447927	20.969059656218402	41.991102123356924	19.7577350859454
16	18.961375126390294	21.30192113245703	36.32639029322548	23.4103134479272
17	19.34236602628918	23.877856420626895	37.050758341759355	19.72901921132457
18	19.663902932254803	20.68190091001011	37.4948432760364	22.159352881698684
19	20.25480283114257	22.55247724974722	34.59292214357937	22.59979777553084
20	23.201617795753286	22.216784630940346	38.22366026289181	16.35793731041456
21	21.190293225480282	26.63215369059656	35.41516683518706	16.762386248736096
22	19.258645096056622	20.459453993933266	39.26673407482305	21.015166835187056
23	21.5943377148635	23.650556117290193	36.25561172901921	18.4994944388271
24	20.28473205257836	23.673205257836198	35.76622851365015	20.27583417593529
25	18.287563195146614	27.64004044489383	34.88493427704753	19.18746208291203
26	17.768655207280084	21.794135490394336	39.811526794742164	20.625682507583416
27	22.236198179979777	22.918099089989887	35.58584428715875	19.25985844287159
28	16.94721941354904	25.085136501516686	38.77654196157735	19.191102123356927
29	19.44550050556117	20.367239635995958	40.37815975733064	19.809100101112236
30	18.4732052578362	26.494641051567243	37.45480283114257	17.577350859453993
31	22.848533872598583	21.70232558139535	33.050353892821036	22.398786653185034
32	23.472194135490394	24.569464105156726	34.879676440849344	17.07866531850354
33	22.858240647118304	24.106370070778567	31.248533872598582	21.78685540950455
34	21.12881698685541	23.380788675429727	33.27927199191102	22.21112234580384
35	21.238827098078865	25.809908998988877	32.34701718907988	20.604246713852376
36	23.547017189079877	26.5840242669363	31.210920121334677	18.65803842264914
37	20.108796764408492	24.83720930232558	36.12173913043478	18.932254802831142
38	22.385035389282105	27.166835187057636	28.56784630940344	21.880283114256823
39	21.556319514661272	25.562386248736097	31.693427704752274	21.187866531850354
40	20.150455005055612	27.663902932254803	31.298281092012132	20.887360970677452
41	21.505763397371084	24.552881698685542	29.062891809908997	24.878463094034377
42	19.908594539939333	25.5267947421638	32.52659251769464	22.038018200202224
43	23.58385798159444	23.72063398740844	30.553718265925216	22.1417897650719
44	20.997770755248524	23.57895854258021	33.06059789487437	22.3626728072969
45	20.623780243828747	23.30458995778317	31.77490344022276	24.296726358165323
46	23.707977103288343	25.704498501588237	31.16941443538048	19.418109959742942
47	18.688093546622245	25.175359088051085	36.33138172345933	19.80516564186734
48	21.154144517119455	24.27222916309416	31.82553097670317	22.748095343083214
49	20.65195201816059	23.40747817708206	35.453565566742604	20.487004238014748
50	21.896817814360254	23.50995810979643	32.874010925749005	21.719213150094312
51	19.66716477629978	23.355217494263574	32.02436654336412	24.953251186072528
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	91.0
1	290.0
2	489.0
3	4876.5
4	9327.0
5	6526.5
6	3615.5
7	3408.5
8	3249.0
9	3196.0
10	3143.0
11	3060.0
12	2977.0
13	2906.0
14	2835.0
15	2612.5
16	2390.0
17	2190.0
18	1986.5
19	1860.5
20	1739.0
21	1578.0
22	1416.0
23	1483.5
24	1551.0
25	1668.0
26	1894.5
27	2006.5
28	2102.0
29	2195.0
30	2694.0
31	3193.0
32	3783.0
33	4373.0
34	4665.0
35	4963.5
36	5818.5
37	6685.0
38	6747.5
39	6829.0
40	8120.5
41	9375.0
42	10584.5
43	11794.0
44	20199.0
45	28604.0
46	24006.0
47	19408.0
48	18961.0
49	18517.0
50	16860.5
51	15168.0
52	14724.5
53	14314.0
54	13435.5
55	12557.0
56	11659.5
57	10762.0
58	9926.5
59	9091.0
60	8482.0
61	7860.0
62	7097.0
63	6344.5
64	5616.5
65	4881.0
66	4171.5
67	3472.0
68	3043.5
69	2615.0
70	2221.5
71	1828.0
72	1644.5
73	1458.0
74	1237.5
75	907.5
76	795.0
77	642.5
78	490.0
79	355.5
80	219.0
81	174.0
82	131.0
83	98.5
84	66.0
85	46.5
86	27.0
87	21.5
88	16.0
89	13.5
90	11.0
91	10.0
92	8.5
93	5.5
94	3.0
95	2.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
42	2324.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	244926.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.320121334681495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.25258437136773	29.982608695652175
2	7.488248181939744	5.888776541961578
3	3.194848743558358	3.7686552072800814
4	2.0572110389944354	3.2355915065722956
5	1.5655376006747652	3.0778564206268957
6	1.3361585698268856	3.1522750252780583
7	1.1201514107324702	3.083114256825076
8	0.9895185097563234	3.112639029322548
9	0.8681430584556517	3.072194135490394
>10	5.061767761445808	30.445702730030334
>50	0.041144220779888704	1.1599595551061679
>100	0.014400477272961046	0.9892821031344793
>500	0.004114422077988871	1.2020222446916078
>1k	0.005143027597486088	5.648938321536906
>5k	0.0010286055194972177	2.1803842264914057
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5391	2.1803842264914057	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTC	3917	1.58422649140546	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGC	3687	1.4912032355915066	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCG	3597	1.4548028311425683	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGTACAGT	1433	0.5795753286147624	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	1333	0.5391304347826087	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTA	864	0.34944388270980786	No Hit
GAACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCT	821	0.33205257836198177	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCT	683	0.27623862487360973	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTC	604	0.2442871587462083	No Hit
CCTGTCTCTTATACACATCTGACGCTTGTACAGTCGTATGCCGTCTTCTGC	249	0.10070778564206267	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08129423660262892	0.0
2	0.0	0.0	0.0	0.8311425682507584	0.0
3	0.0	0.0	0.0	0.9577350859453994	0.0
4	0.0	0.0	0.0	1.4580384226491405	0.0
5	0.0	0.0	0.0	3.4693629929221435	0.0
6	0.0	0.0	0.0	3.8390293225480283	0.0
7	0.0	0.0	0.0	4.373306370070779	0.0
8	0.0	0.0	0.0	4.985642062689585	0.0
9	0.0	0.0	0.0	5.1389282103134475	0.0
10	0.0	0.0	0.0	7.022851365015167	0.0
11	0.0	0.0	0.0	7.550657229524773	0.0
12	0.0	0.0	0.0	9.641253791708797	0.0
13	0.0	0.0	0.0	9.943377148634985	0.0
14	0.0	0.0	0.0	10.090192113245703	0.0
15	0.0	0.0	0.0	10.603437815975733	0.0
16	0.0	0.0	0.0	11.007077856420628	0.0
17	0.0	0.0	0.0	11.370677451971689	0.0
18	0.0	0.0	0.0	11.745197168857432	0.0
19	0.0	0.0	0.0	12.59211324570273	0.0
20	0.0	0.0	0.0	12.879271991911022	0.0
21	0.0	0.0	0.0	13.164004044489383	0.0
22	0.0	0.0	0.0	13.499696663296259	0.0
23	0.0	0.0	0.0	13.796157735085945	0.0
24	0.0	0.0	0.0	14.038422649140546	0.0
25	0.0	0.0	0.0	14.262082912032357	0.0
26	0.0	0.0	0.0	14.464711830131446	0.0
27	0.0	0.0	0.0	14.727603640040446	0.0
28	4.044489383215369E-4	0.0	0.0	14.963397371081902	0.0
29	4.044489383215369E-4	0.0	0.0	15.166430738119313	0.0
30	4.044489383215369E-4	0.0	0.0	15.409908998988877	0.0
31	4.044489383215369E-4	0.0	0.0	15.648533872598584	0.0
32	4.044489383215369E-4	0.0	0.0	15.895247724974721	0.0
33	4.044489383215369E-4	0.0	0.0	16.14196157735086	0.0
34	4.044489383215369E-4	0.0	0.0	16.386248736097066	0.0
35	4.044489383215369E-4	0.0	0.0	16.63417593528817	0.0
36	4.044489383215369E-4	0.0	0.0	16.88372093023256	0.0
37	4.044489383215369E-4	0.0	0.0	17.113447927199193	0.0
38	4.044489383215369E-4	0.0	0.0	17.356926188068755	0.0
39	4.044489383215369E-4	0.0	0.0	17.614964610717898	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAAGG	20	7.088668E-4	44.91446	2
GCGAACC	40	6.892151E-9	44.91446	33
CGTTGAT	45	3.8926373E-10	44.91446	25
ACGGGTC	30	2.1870273E-6	44.91446	5
ACGGGCG	30	2.1870273E-6	44.91446	5
AACGGGC	35	1.2249438E-7	44.91446	4
TGACCCA	30	2.1870273E-6	44.91446	11
ACAGCGG	35	1.2249438E-7	44.91446	2
GCGATTA	20	7.088668E-4	44.91446	9
GCGATAC	25	3.9257582E-5	44.91446	33
GGCATTA	20	7.088668E-4	44.91446	7
ATAACGC	20	7.088668E-4	44.91446	11
GCTACGA	30	2.1870273E-6	44.91446	10
GTAAGCG	20	7.088668E-4	44.91446	1
TAAGCGG	25	3.9257582E-5	44.91446	2
GGCCTAT	45	3.8926373E-10	44.91446	8
GCCAATC	20	7.088668E-4	44.91446	15
AATCGTT	45	3.8926373E-10	44.91446	22
AGTAACG	20	7.088668E-4	44.91446	1
TAATCGT	45	3.8926373E-10	44.91446	21
>>END_MODULE