FastQCFastQC Report
Sat 18 Jun 2016
SRR3552037_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552037_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509170
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21840.42893336213838207No Hit
GCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC10970.2154486713671269No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCC6400.12569475813578962No Hit
CTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGCT5440.10684054441542118No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5220.10251978710450341No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAAT850.045.00000412
GTACCGG207.029581E-445.02
ACGATAG207.029581E-445.01
TCATCGG207.029581E-445.02
CGCCGTT207.029581E-445.026
TAGTGCG406.8030204E-945.01
CGTCATA207.029581E-445.038
CGTTTAC207.029581E-445.033
TACCGGT207.029581E-445.040
CGTAGCG302.1631113E-644.9999962
CGTAAGG302.1631113E-644.9999962
GTACTAG501.0786607E-940.51
CGAAAGG356.241433E-638.571432
CATACGG356.241433E-638.571432
GCGATCT356.241433E-638.571439
CGTTTTT11800.038.516951
CGAATAT1000.038.2514
TAACGGG950.037.8947373
ACGGGAT1500.037.55
AGATACG301.1390292E-437.4999961