##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552037_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	509170
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.356613704656596	33.0	31.0	34.0	30.0	34.0
2	32.42786692067482	34.0	31.0	34.0	31.0	34.0
3	32.44191527387709	34.0	31.0	34.0	30.0	34.0
4	35.9721232594222	37.0	35.0	37.0	35.0	37.0
5	36.03923444036373	37.0	35.0	37.0	35.0	37.0
6	36.01942966003496	37.0	35.0	37.0	35.0	37.0
7	36.20074238466524	37.0	36.0	37.0	35.0	37.0
8	36.04398138146395	37.0	36.0	37.0	35.0	37.0
9	37.917740636722506	39.0	38.0	39.0	35.0	39.0
10	37.60305398982658	39.0	37.0	39.0	35.0	39.0
11	37.56002906691282	39.0	37.0	39.0	35.0	39.0
12	37.45145432763124	39.0	37.0	39.0	35.0	39.0
13	37.43765343598405	39.0	37.0	39.0	35.0	39.0
14	38.68714574700002	40.0	38.0	41.0	35.0	41.0
15	38.75910599603276	40.0	38.0	41.0	35.0	41.0
16	38.740440324449594	40.0	38.0	41.0	35.0	41.0
17	38.69223049276273	40.0	38.0	41.0	35.0	41.0
18	38.61735569652572	40.0	38.0	41.0	35.0	41.0
19	38.56863522988393	40.0	38.0	41.0	35.0	41.0
20	38.527485908439225	40.0	38.0	41.0	35.0	41.0
21	38.40412239527073	40.0	37.0	41.0	34.0	41.0
22	38.428505214368485	40.0	37.0	41.0	34.0	41.0
23	38.3991888760139	40.0	37.0	41.0	34.0	41.0
24	38.331818449633715	40.0	37.0	41.0	34.0	41.0
25	38.16539269792015	40.0	37.0	41.0	34.0	41.0
26	38.185441011842805	40.0	37.0	41.0	34.0	41.0
27	38.187458019914764	40.0	37.0	41.0	34.0	41.0
28	38.136435768014614	40.0	37.0	41.0	34.0	41.0
29	38.09028615197282	40.0	36.0	41.0	34.0	41.0
30	37.91462969145865	40.0	36.0	41.0	34.0	41.0
31	37.889473064006125	40.0	36.0	41.0	34.0	41.0
32	37.784979476402775	40.0	36.0	41.0	33.0	41.0
33	37.683076379205374	40.0	36.0	41.0	33.0	41.0
34	37.56225032896675	40.0	36.0	41.0	33.0	41.0
35	37.44763634935287	40.0	35.0	41.0	33.0	41.0
36	37.372480703890645	40.0	35.0	41.0	33.0	41.0
37	37.36133707798967	40.0	35.0	41.0	33.0	41.0
38	37.26273543217393	40.0	35.0	41.0	33.0	41.0
39	37.207190132961486	40.0	35.0	41.0	33.0	41.0
40	37.105752499165305	40.0	35.0	41.0	32.0	41.0
41	37.026957597658935	39.0	35.0	41.0	32.0	41.0
42	36.94991456684408	39.0	35.0	41.0	32.0	41.0
43	36.82198283480959	39.0	35.0	41.0	32.0	41.0
44	36.816776322249936	39.0	35.0	41.0	32.0	41.0
45	36.77296777107842	39.0	35.0	41.0	32.0	41.0
46	36.68091403656932	39.0	35.0	41.0	32.0	41.0
47	36.61457666398256	39.0	35.0	41.0	32.0	41.0
48	36.56841722803779	39.0	35.0	41.0	31.0	41.0
49	36.527599819313785	39.0	35.0	41.0	31.0	41.0
50	36.43783215821828	38.0	35.0	41.0	31.0	41.0
51	35.59708938075692	37.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	4.0
10	4.0
11	12.0
12	6.0
13	17.0
14	16.0
15	23.0
16	41.0
17	72.0
18	103.0
19	181.0
20	308.0
21	512.0
22	732.0
23	1128.0
24	1682.0
25	2557.0
26	3611.0
27	4339.0
28	4777.0
29	5153.0
30	6100.0
31	7430.0
32	9694.0
33	13678.0
34	27598.0
35	40099.0
36	34762.0
37	52866.0
38	95922.0
39	195498.0
40	242.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.555845788243616	19.830704872635856	23.480173615884674	12.133275723235855
2	33.46799693619027	23.227409313195984	24.55682777854155	18.747765972072198
3	33.31480644971228	23.385509751163656	25.734626941885814	17.56505685723825
4	29.40432468527211	25.643891038356543	25.294302492291376	19.657481784079973
5	25.739144097256318	29.7305418622464	23.93326393935228	20.597050101145
6	26.857041852426498	34.29286878645639	23.421057799948937	15.429031561168175
7	79.53414380265923	6.508042500540094	9.364652277235502	4.593161419565175
8	79.5469096765324	5.967162244437025	9.270774004752832	5.215154074277746
9	72.57536775536659	8.24616532788656	11.872851896223265	7.305615020523597
10	39.37781094722784	26.37802698509339	18.680401437633794	15.563760630044976
11	32.19356992752911	24.53659877840407	23.725278394249464	19.54455289981735
12	28.166820511813345	22.63291238682562	29.134866547518513	20.065400553842526
13	25.887228234185045	23.46937172260738	29.629986055737767	21.013413987469804
14	21.73517685645266	26.37586660643793	29.351100811123988	22.537855725985427
15	21.308011076850562	24.973388062925938	32.525679046291025	21.19292181393248
16	23.698175462026434	23.884360822515074	30.751811772099693	21.6656519433588
17	23.771824734371624	23.88809238564723	28.30096038651138	24.039122493469765
18	24.497908360665395	24.171887581750692	28.683151010468016	22.647053047115897
19	25.300980026317337	25.341634424651883	27.00689357189151	22.350491977139267
20	26.95268770744545	24.499283147082508	27.332914350806213	21.21511479466583
21	26.228764459807135	25.969322623092484	26.782803385902547	21.019109531197834
22	24.530706836616453	24.61653278865605	26.372527839424947	24.48023253530255
23	23.767503977060706	26.300646149616043	27.265157020248644	22.66669285307461
24	23.788714967496123	24.08802561030697	28.81552330262977	23.30773611956714
25	23.81797827837461	25.61757369837186	26.92911994029499	23.63532808295854
26	22.739949329300625	26.1527584107469	27.75124221772689	23.356050042225583
27	23.572480703890644	25.551976746469744	27.59844452736807	23.277098022271538
28	21.701592788263252	26.639236404344324	28.308423512775693	23.35074729461673
29	24.25614234931359	25.817899719150777	26.5661763261779	23.35978160535774
30	23.526719956006833	25.154270675805723	27.83824655812401	23.480762810063435
31	24.49692637036746	25.804151854979672	25.43688748355166	24.2620342911012
32	24.752833042009545	26.186735275055483	25.457705677867903	23.60272600506707
33	23.970186774554666	26.463460141013805	25.67177956281792	23.89457352161361
34	23.00783628257753	26.278845965001867	26.532395859928904	24.180921892491703
35	22.904530903234676	25.49050415381896	26.623131763458176	24.981833179488184
36	22.926723883968027	28.147966298092975	26.034919574994596	22.8903902429444
37	23.074808020896754	27.35903529273131	26.09855254630084	23.467604140071096
38	21.916845061570793	27.21802148594772	25.68611662116778	25.179016831313707
39	23.383742168627375	26.262152129936954	26.41239664552114	23.94170905591453
40	23.86157864760296	25.13718404462164	28.096706404540722	22.904530903234676
41	20.987685841663883	26.357994383015498	27.805448082172944	24.84887169314767
42	22.651766600545987	25.236561462772748	27.381228273464657	24.730443663216608
43	23.21582182768034	24.743995129328123	27.215271913113497	24.824911129878036
44	22.39036078323546	25.476167095469098	26.86450497869081	25.26896714260463
45	23.31657403224856	25.017577626333054	25.797867117072883	25.867981224345506
46	23.017852583616474	26.890625920615907	25.81043659288646	24.281084902881158
47	22.057466072235208	26.159043148653694	28.048196083822692	23.73529469528841
48	21.756387846888074	24.548579060038886	28.43097590195809	25.26405719111495
49	22.121099043541452	24.914272246990198	28.514248679223048	24.4503800302453
50	21.00948602627806	25.22222440442288	28.354184260659505	25.414105308639552
51	20.82781782115993	24.699412769801835	27.011017931142838	27.461751477895397
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	253.0
1	612.5
2	972.0
3	2393.0
4	3814.0
5	2587.5
6	1361.0
7	1367.5
8	1374.0
9	1345.0
10	1316.0
11	1334.0
12	1352.0
13	1356.0
14	1360.0
15	1289.0
16	1218.0
17	1305.0
18	1392.0
19	1352.5
20	1313.0
21	1415.0
22	1517.0
23	1818.0
24	2119.0
25	2490.0
26	3694.0
27	4527.0
28	5136.0
29	5745.0
30	6283.5
31	6822.0
32	7796.5
33	8771.0
34	10318.5
35	11866.0
36	12235.0
37	12604.0
38	14126.0
39	15648.0
40	17890.0
41	20132.0
42	22091.0
43	24050.0
44	26486.0
45	28922.0
46	33014.0
47	37106.0
48	40062.5
49	43019.0
50	43242.5
51	43466.0
52	40000.5
53	36535.0
54	34069.5
55	31604.0
56	30127.0
57	28650.0
58	27371.5
59	26093.0
60	26202.5
61	26312.0
62	24067.5
63	21823.0
64	19478.5
65	17134.0
66	14524.5
67	11915.0
68	9861.0
69	7807.0
70	6542.5
71	5278.0
72	4843.0
73	4408.0
74	3681.5
75	2446.0
76	1937.0
77	1473.5
78	1010.0
79	717.0
80	424.0
81	346.0
82	268.0
83	171.0
84	74.0
85	50.5
86	27.0
87	18.5
88	10.0
89	6.0
90	2.0
91	2.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	509170.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.024956935265802
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.92766429681117	21.596250229660107
2	8.329487947274371	5.00185033819457
3	3.157612161971716	2.8442150754441697
4	1.7818929586808334	2.1400503739058157
5	1.3742280332468941	2.0630568758736505
6	1.1399095153130627	2.053544046442466
7	0.9812237923177419	2.062284147573888
8	0.8786211969434523	2.1104450880511076
9	0.8239471812092265	2.2265080769466605
>10	9.47344204364069	52.94975671665421
>50	0.0879805817272327	1.8203474687338141
>100	0.04070743333648081	2.1485777628037517
>500	0.0019697145162813293	0.33631284185183963
>1k	0.0013131430108542196	0.6468009578639424
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2184	0.42893336213838207	No Hit
GCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGC	1097	0.2154486713671269	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCC	640	0.12569475813578962	No Hit
CTGTCTCTTATACACATCTGACGCGTCCAACTTCGTATGCCGTCTTCTGCT	544	0.10684054441542118	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	522	0.10251978710450341	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.11135769978592612	0.0
2	0.0	0.0	0.0	0.5416658483414184	0.0
3	0.0	0.0	0.0	0.7588821022448298	0.0
4	0.0	0.0	0.0	1.0923660074238466	0.0
5	0.0	0.0	0.0	2.027417169118369	0.0
6	0.0	0.0	0.0	2.617789736237406	0.0
7	0.0	0.0	0.0	3.1940216430661663	0.0
8	0.0	0.0	0.0	4.141838678633855	0.0
9	0.0	0.0	0.0	4.586876681658385	0.0
10	0.0	0.0	0.0	5.448082172948132	0.0
11	0.0	0.0	0.0	6.479957578019129	0.0
12	0.0	0.0	0.0	7.297366302020936	0.0
13	0.0	0.0	0.0	7.615923954671328	0.0
14	0.0	0.0	0.0	7.755955771156981	0.0
15	0.0	0.0	0.0	8.0511420547165	0.0
16	0.0	0.0	0.0	8.579060038886816	0.0
17	0.0	0.0	0.0	9.193785965394662	0.0
18	0.0	0.0	0.0	9.888053106035313	0.0
19	0.0	0.0	0.0	10.282420409686353	0.0
20	0.0	0.0	0.0	10.670895771549777	0.0
21	0.0	0.0	0.0	11.112595007561325	0.0
22	0.0	0.0	0.0	11.589645894298565	0.0
23	0.0	0.0	0.0	12.074356305359704	0.0
24	0.0	0.0	0.0	12.475008346917532	0.0
25	0.0	0.0	0.0	12.820472533731367	0.0
26	0.0	0.0	0.0	13.165151128306853	0.0
27	0.0	0.0	0.0	13.487243946029814	0.0
28	0.0	0.0	0.0	13.837618084333327	0.0
29	0.0	0.0	0.0	14.166781232201426	0.0
30	0.0	0.0	0.0	14.577449574798202	0.0
31	0.0	0.0	0.0	14.970442092032131	0.0
32	0.0	0.0	0.0	15.34124162853271	0.0
33	0.0	0.0	0.0	15.698293300862188	0.0
34	0.0	0.0	0.0	16.04532867215272	0.0
35	0.0	0.0	0.0	16.41534261641495	0.0
36	0.0	0.0	0.0	16.781624997545023	0.0
37	0.0	0.0	0.0	17.14731818449634	0.0
38	0.0	0.0	0.0	17.54089989590903	0.0
39	0.0	0.0	0.0	17.92387611210401	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAAT	85	0.0	45.000004	12
GTACCGG	20	7.029581E-4	45.0	2
ACGATAG	20	7.029581E-4	45.0	1
TCATCGG	20	7.029581E-4	45.0	2
CGCCGTT	20	7.029581E-4	45.0	26
TAGTGCG	40	6.8030204E-9	45.0	1
CGTCATA	20	7.029581E-4	45.0	38
CGTTTAC	20	7.029581E-4	45.0	33
TACCGGT	20	7.029581E-4	45.0	40
CGTAGCG	30	2.1631113E-6	44.999996	2
CGTAAGG	30	2.1631113E-6	44.999996	2
GTACTAG	50	1.0786607E-9	40.5	1
CGAAAGG	35	6.241433E-6	38.57143	2
CATACGG	35	6.241433E-6	38.57143	2
GCGATCT	35	6.241433E-6	38.57143	9
CGTTTTT	1180	0.0	38.51695	1
CGAATAT	100	0.0	38.25	14
TAACGGG	95	0.0	37.894737	3
ACGGGAT	150	0.0	37.5	5
AGATACG	30	1.1390292E-4	37.499996	1
>>END_MODULE