FastQCFastQC Report
Sat 18 Jun 2016
SRR3552036_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552036_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282079
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14450.5122678398604646No Hit
GCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC10520.37294516784305104TruSeq Adapter, Index 21 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCG10340.366563976758284No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGC7850.27829083341900673No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTC7800.2765182803399048No Hit
CCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC6220.22050560304028305TruSeq Adapter, Index 21 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGCT6220.22050560304028305Illumina Paired End PCR Primer 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCC5290.1875361157689867No Hit
TCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC3850.13648658709085043TruSeq Adapter, Index 21 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA3580.12691480046369988No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG3180.11273437583088426No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG2900.1028080785879133No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGA302.159657E-645.00000413
GTGTTAG406.7830115E-945.0000041
CGACGGA207.0242246E-445.0000044
ACTTCGG207.0242246E-445.00000412
GCCTAAA207.0242246E-445.00000412
CGTCATG207.0242246E-445.00000436
AGTACGG207.0242246E-445.0000042
GTAGTTT207.0242246E-445.0000048
TCGTTTA253.8832975E-545.038
CGCATCG253.8832975E-545.021
ACACAAC253.8832975E-545.011
GTACTAG351.2075725E-745.01
ATAACGC253.8832975E-545.011
CACAACG253.8832975E-545.012
CTAGGGT453.8198777E-1045.04
CGACCTC1000.042.7512
TGTTAGG700.041.7857132
CGTTTTT6900.041.0869561
AACGGGC501.0750227E-940.54
TGAATGG501.0750227E-940.52
Produced by FastQC (version 0.11.5)