##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552036_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	282079
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30586466911752	33.0	31.0	34.0	30.0	34.0
2	32.38779207243361	34.0	31.0	34.0	31.0	34.0
3	32.388299022614234	34.0	31.0	34.0	30.0	34.0
4	35.94284934362359	37.0	35.0	37.0	35.0	37.0
5	36.00705121614867	37.0	35.0	37.0	35.0	37.0
6	35.99498012967998	37.0	35.0	37.0	35.0	37.0
7	36.16911574417096	37.0	36.0	37.0	35.0	37.0
8	35.991860436260765	37.0	35.0	37.0	35.0	37.0
9	37.89262582468032	39.0	38.0	39.0	35.0	39.0
10	37.59470928356949	39.0	37.0	39.0	35.0	39.0
11	37.56550824414437	39.0	37.0	39.0	35.0	39.0
12	37.488033494162984	39.0	37.0	39.0	35.0	39.0
13	37.456914552306266	39.0	37.0	39.0	35.0	39.0
14	38.68817600743054	40.0	38.0	41.0	35.0	41.0
15	38.74855980062323	40.0	38.0	41.0	35.0	41.0
16	38.76889098444053	40.0	38.0	41.0	35.0	41.0
17	38.7249316680788	40.0	38.0	41.0	35.0	41.0
18	38.652154183757034	40.0	38.0	41.0	35.0	41.0
19	38.576831313213674	40.0	38.0	41.0	35.0	41.0
20	38.48357729572212	40.0	37.0	41.0	35.0	41.0
21	38.39872163471935	40.0	37.0	41.0	34.0	41.0
22	38.42552972748769	40.0	37.0	41.0	34.0	41.0
23	38.377755876899734	40.0	37.0	41.0	34.0	41.0
24	38.316485807167496	40.0	37.0	41.0	34.0	41.0
25	38.14429291085121	40.0	37.0	41.0	34.0	41.0
26	38.1775601870398	40.0	37.0	41.0	34.0	41.0
27	38.18268995565072	40.0	37.0	41.0	34.0	41.0
28	38.118261196331524	40.0	37.0	41.0	34.0	41.0
29	38.082193286277956	40.0	37.0	41.0	34.0	41.0
30	37.93537980494826	40.0	36.0	41.0	34.0	41.0
31	37.89776977371587	40.0	36.0	41.0	34.0	41.0
32	37.75451203386285	40.0	36.0	41.0	33.0	41.0
33	37.67120558425122	40.0	36.0	41.0	33.0	41.0
34	37.56185678480142	40.0	36.0	41.0	33.0	41.0
35	37.400394924826024	40.0	36.0	41.0	33.0	41.0
36	37.333534222682296	40.0	35.0	41.0	33.0	41.0
37	37.29135809471815	40.0	35.0	41.0	33.0	41.0
38	37.16455673765151	40.0	35.0	41.0	32.0	41.0
39	37.12993523091049	40.0	35.0	41.0	33.0	41.0
40	36.99119395630302	39.0	35.0	41.0	32.0	41.0
41	36.87532925173444	39.0	35.0	41.0	32.0	41.0
42	36.77684620265954	39.0	35.0	41.0	31.0	41.0
43	36.69170693316411	39.0	35.0	41.0	31.0	41.0
44	36.73903764548229	39.0	35.0	41.0	31.0	41.0
45	36.69069657790902	39.0	35.0	41.0	31.0	41.0
46	36.6240733978779	39.0	35.0	41.0	31.0	41.0
47	36.53294998918743	39.0	35.0	41.0	31.0	41.0
48	36.48486416925755	39.0	35.0	41.0	31.0	41.0
49	36.44024191804424	39.0	35.0	41.0	31.0	41.0
50	36.362483559570194	38.0	35.0	41.0	31.0	41.0
51	35.50110430056828	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	4.0
10	10.0
11	5.0
12	4.0
13	8.0
14	9.0
15	17.0
16	18.0
17	43.0
18	55.0
19	122.0
20	173.0
21	338.0
22	536.0
23	706.0
24	1012.0
25	1479.0
26	1998.0
27	2507.0
28	2715.0
29	2862.0
30	3240.0
31	4197.0
32	5440.0
33	7641.0
34	14544.0
35	21592.0
36	19806.0
37	30404.0
38	55652.0
39	104803.0
40	135.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.18967381478238	19.59628331070374	23.700098199440582	13.513944675073294
2	32.69119643787734	25.859422360402583	24.741295878105067	16.708085323615016
3	32.072929923886576	25.025613391993023	26.713792944529725	16.187663739590683
4	29.54704178616629	24.89444446413948	27.255839676119102	18.302674073575133
5	25.96542103453288	29.15353500260565	24.711516986376157	20.16952697648531
6	26.197625487895237	34.53748772506992	23.94471052435665	15.320176262678187
7	76.90717848545975	6.889204797237654	11.27698268924663	4.92663402805597
8	76.83095870305836	7.472020249646376	10.231885393808117	5.465135653487144
9	71.03754621932154	8.114393485512924	12.437295934826768	8.410764360338769
10	39.2102921521985	25.223784826236624	19.48106736056211	16.08485566100277
11	30.100787368077736	24.166634169860217	24.861829487484002	20.870748974578042
12	27.293417801396064	21.66485275401572	30.488621981785247	20.553107462802974
13	25.03518517862017	23.27362192860865	31.84852470407226	19.842668188698912
14	21.010071646595456	27.174656745096232	29.492801661945766	22.322469946362546
15	19.71468985638775	24.183296168803775	34.193257917108326	21.90875605770015
16	21.542546591557684	23.220445336235592	31.65283484413941	23.584173228067314
17	21.98320328702243	23.79900666125447	28.895805784904226	25.32198426681887
18	22.223561484548654	24.09821362100688	30.26208969827601	23.41613519616845
19	23.930884610339657	25.315603075734106	27.41820553816484	23.3353067757614
20	25.05042913510045	24.97704543762563	28.528887297530126	21.443638129743796
21	24.087223791916447	25.64707050152617	28.057742689104824	22.20796301745256
22	22.805313405109917	24.54064286955073	26.91515497431571	25.738888751023648
23	22.779079619539207	25.6580603306166	27.85496261685556	23.70789743298863
24	22.81878480851109	24.701236178517366	28.919912506780015	23.560066506191525
25	22.4086160260069	26.101198600392088	27.45188404666778	24.038301326933233
26	20.88351135674758	26.427348366946845	28.67813626679051	24.011004009515066
27	23.384583751360434	25.282279077846987	28.54980342386353	22.783333746929053
28	21.109689129640987	25.064255049117445	29.22975478500704	24.59630103623453
29	21.814810744507746	24.562268017115773	28.669273501394997	24.953647736981484
30	23.549076677101098	24.910751952467216	28.754001538576073	22.786169831855617
31	24.32155530897373	25.053265220027015	25.180534531106535	25.444644939892726
32	23.77879955615271	25.79738300263401	26.84637991484655	23.577437526366726
33	24.237181782408474	24.114521109334618	26.897074932908865	24.75122217534804
34	22.654646393386248	24.24249944164578	28.0921302188394	25.010723946128564
35	22.870543358420868	23.178258572952966	29.341425628990457	24.609772439635705
36	23.69726211451402	25.00363373381216	28.574264656355137	22.724839495318687
37	23.11586470456858	25.505266255198013	28.060224263415567	23.31864477681784
38	22.276383566305892	26.15366617153351	26.46669904530291	25.103251216857693
39	25.1504011287618	23.23746184579497	27.633038971351997	23.97909805409123
40	24.60551831224586	22.804604383878274	30.143328641976186	22.446548661899683
41	21.597850247625665	24.19960365713151	30.07880770989687	24.12373838534595
42	21.196189719901163	24.366578157182918	30.768685368283354	23.668546754632565
43	22.705695922064386	23.002421307506054	29.3595056703973	24.93237710003226
44	23.463639618688383	22.759227025053267	28.06518741203705	25.7119459442213
45	23.513625615519057	23.18889389142758	26.954505652671767	26.342974840381594
46	24.292485438476454	23.601189737626694	28.58773605975631	23.518588764140542
47	21.98391230825407	23.986188266407638	31.431265709251665	22.598633716086628
48	22.160104084316806	22.771280385991158	29.794490196008923	25.274125333683116
49	21.669461392021383	22.985759308562496	31.359654564855944	23.985124734560177
50	21.987457414412276	22.870543358420868	30.26847088936078	24.873528337806075
51	21.860188103332753	22.376001049351423	28.363330839942	27.400480007373822
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	147.0
1	353.0
2	559.0
3	1478.0
4	2397.0
5	1705.0
6	1013.0
7	939.5
8	866.0
9	887.5
10	909.0
11	894.5
12	880.0
13	862.0
14	844.0
15	845.5
16	847.0
17	848.0
18	849.0
19	840.5
20	832.0
21	889.5
22	947.0
23	1047.5
24	1148.0
25	1502.5
26	2053.0
27	2249.0
28	2578.0
29	2907.0
30	3500.5
31	4094.0
32	4529.5
33	4965.0
34	5394.5
35	5824.0
36	6321.5
37	6819.0
38	7434.5
39	8050.0
40	9037.5
41	10025.0
42	11389.0
43	12753.0
44	14512.5
45	16272.0
46	20622.0
47	24972.0
48	26085.0
49	27198.0
50	26748.0
51	26298.0
52	23352.0
53	20406.0
54	18248.5
55	16091.0
56	15078.5
57	14066.0
58	13769.5
59	13473.0
60	12886.0
61	12299.0
62	11820.0
63	11341.0
64	9857.0
65	8373.0
66	7191.0
67	6009.0
68	5342.5
69	4676.0
70	3980.5
71	3285.0
72	2766.5
73	2248.0
74	1907.0
75	1164.5
76	763.0
77	623.5
78	484.0
79	358.5
80	233.0
81	181.5
82	130.0
83	107.5
84	85.0
85	51.0
86	17.0
87	12.5
88	8.0
89	5.5
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	282079.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.30032012308609
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.20063253198833	24.765048089365035
2	8.56088637162288	5.872822861680593
3	3.246377410753044	3.340553532875542
4	2.022655394092234	2.7751091006420188
5	1.553424147838849	2.6641472778902364
6	1.2609297806809021	2.5950177078052605
7	1.1565413316245323	2.7768816537211207
8	1.038716745560907	2.8502653511959415
9	0.9643012175207226	2.976825641043821
>10	7.881844678256196	42.97058625420539
>50	0.06408003803460322	1.5481478592876463
>100	0.04134196002232466	2.4294612502171375
>500	0.0051677450027905825	1.1833564356084643
>1k	0.0031006470016743497	1.2517769844617996
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1445	0.5122678398604646	No Hit
GCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC	1052	0.37294516784305104	TruSeq Adapter, Index 21 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCG	1034	0.366563976758284	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGC	785	0.27829083341900673	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTC	780	0.2765182803399048	No Hit
CCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC	622	0.22050560304028305	TruSeq Adapter, Index 21 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGCT	622	0.22050560304028305	Illumina Paired End PCR Primer 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCC	529	0.1875361157689867	No Hit
TCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC	385	0.13648658709085043	TruSeq Adapter, Index 21 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	358	0.12691480046369988	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	318	0.11273437583088426	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	290	0.1028080785879133	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2265322835092297	0.0
2	0.0	0.0	0.0	1.0649498899244538	0.0
3	0.0	0.0	0.0	1.4293868029878154	0.0
4	0.0	0.0	0.0	1.9359824729951538	0.0
5	0.0	0.0	0.0	3.5248990531021454	0.0
6	0.0	0.0	0.0	4.311203598991772	0.0
7	0.0	0.0	0.0	5.138985886932384	0.0
8	0.0	0.0	0.0	6.283700665416426	0.0
9	0.0	0.0	0.0	6.820075227152677	0.0
10	0.0	0.0	0.0	8.207984288089508	0.0
11	0.0	0.0	0.0	9.607237688732589	0.0
12	0.0	0.0	0.0	10.934170923748312	0.0
13	0.0	0.0	0.0	11.381563320913644	0.0
14	0.0	0.0	0.0	11.564845309292787	0.0
15	0.0	0.0	0.0	11.964733283938187	0.0
16	0.0	0.0	0.0	12.672336473115687	0.0
17	0.0	0.0	0.0	13.50047327167212	0.0
18	0.0	0.0	0.0	14.45304329638151	0.0
19	0.0	0.0	0.0	14.982682156417173	0.0
20	0.0	0.0	0.0	15.4612714877747	0.0
21	0.0	0.0	0.0	16.009699410448846	0.0
22	0.0	0.0	0.0	16.644273412767344	0.0
23	0.0	0.0	0.0	17.235597119955756	0.0
24	0.0	0.0	0.0	17.733330024567586	0.0
25	0.0	0.0	0.0	18.12967289305478	0.0
26	0.0	0.0	0.0	18.521052612920492	0.0
27	0.0	0.0	0.0	18.90746918416472	0.0
28	0.0	0.0	0.0	19.285022990013434	0.0
29	0.0	0.0	0.0	19.67746624172661	0.0
30	0.0	0.0	0.0	20.12379510704448	0.0
31	0.0	0.0	0.0	20.538572527554336	0.0
32	0.0	0.0	0.0	20.95654054360658	0.0
33	3.5451061582039077E-4	0.0	0.0	21.325940605291425	0.0
34	3.5451061582039077E-4	0.0	0.0	21.734336834716515	0.0
35	3.5451061582039077E-4	0.0	0.0	22.160104084316806	0.0
36	3.5451061582039077E-4	0.0	0.0	22.550774782950874	0.0
37	3.5451061582039077E-4	0.0	0.0	22.939672928505843	0.0
38	3.5451061582039077E-4	0.0	0.0	23.322898904207687	0.0
39	3.5451061582039077E-4	0.0	0.0	23.71640568776832	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGA	30	2.159657E-6	45.000004	13
GTGTTAG	40	6.7830115E-9	45.000004	1
CGACGGA	20	7.0242246E-4	45.000004	4
ACTTCGG	20	7.0242246E-4	45.000004	12
GCCTAAA	20	7.0242246E-4	45.000004	12
CGTCATG	20	7.0242246E-4	45.000004	36
AGTACGG	20	7.0242246E-4	45.000004	2
GTAGTTT	20	7.0242246E-4	45.000004	8
TCGTTTA	25	3.8832975E-5	45.0	38
CGCATCG	25	3.8832975E-5	45.0	21
ACACAAC	25	3.8832975E-5	45.0	11
GTACTAG	35	1.2075725E-7	45.0	1
ATAACGC	25	3.8832975E-5	45.0	11
CACAACG	25	3.8832975E-5	45.0	12
CTAGGGT	45	3.8198777E-10	45.0	4
CGACCTC	100	0.0	42.75	12
TGTTAGG	70	0.0	41.785713	2
CGTTTTT	690	0.0	41.086956	1
AACGGGC	50	1.0750227E-9	40.5	4
TGAATGG	50	1.0750227E-9	40.5	2
>>END_MODULE