SRR3552035_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3552035_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	448964
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2530	0.5635195694977772	No Hit
GCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	1321	0.2942329451804599	Illumina PCR Primer Index 6 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGCT	862	0.19199757664311615	RNA PCR Primer, Index 27 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	794	0.17685159611906523	RNA PCR Primer, Index 27 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCC	792	0.17640612610365197	No Hit
TCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	524	0.11671314403827479	Illumina PCR Primer Index 6 (95% over 22bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	473	0.10535365864523659	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTC	449	0.10000801846027745	RNA PCR Primer, Index 27 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAG	25	3.8869952E-5	45.0	1
TCTAGCG	20	7.028689E-4	45.0	1
ATACTCG	20	7.028689E-4	45.0	44
CCCAACG	20	7.028689E-4	45.0	31
GTGCTCG	20	7.028689E-4	45.0	1
TACGTTG	25	3.8869952E-5	45.0	1
GTACAAC	30	2.1625347E-6	44.999996	14
GTCTTAC	65	0.0	41.538464	31
TCGTTCA	50	1.0786607E-9	40.5	16
CACGACG	50	1.0786607E-9	40.5	26
CGTTAGG	50	1.0786607E-9	40.5	2
TGTTGCG	50	1.0786607E-9	40.5	1
CTAGCGG	95	0.0	40.263157	2
TAGCGGG	270	0.0	40.000004	3
TTAGTCA	45	1.9237632E-8	40.000004	32
CACAACG	45	1.9237632E-8	40.000004	12
GCGTAAG	45	1.9237632E-8	40.000004	1
CGTTTTT	1210	0.0	39.79339	1
GTAGTCA	85	0.0	39.705883	9
TTCGGCA	40	3.4519508E-7	39.375	18