##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552035_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	448964
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23699004819986	33.0	31.0	34.0	30.0	34.0
2	32.31965146425994	34.0	31.0	34.0	30.0	34.0
3	32.32895287818177	34.0	31.0	34.0	30.0	34.0
4	35.88777273901694	37.0	35.0	37.0	35.0	37.0
5	35.9663670138363	37.0	35.0	37.0	35.0	37.0
6	35.936769986012244	37.0	35.0	37.0	35.0	37.0
7	36.13304184745325	37.0	35.0	37.0	35.0	37.0
8	35.961359930863054	37.0	35.0	37.0	35.0	37.0
9	37.85669452339163	39.0	38.0	39.0	35.0	39.0
10	37.525414064379326	39.0	37.0	39.0	35.0	39.0
11	37.472300674441605	39.0	37.0	39.0	35.0	39.0
12	37.392808777541184	39.0	37.0	39.0	35.0	39.0
13	37.37144626295204	39.0	37.0	39.0	35.0	39.0
14	38.5901965413708	40.0	38.0	41.0	35.0	41.0
15	38.65094974207286	40.0	38.0	41.0	35.0	41.0
16	38.65222601366702	40.0	38.0	41.0	35.0	41.0
17	38.63169875535678	40.0	38.0	41.0	35.0	41.0
18	38.54508156555982	40.0	38.0	41.0	35.0	41.0
19	38.46784374693739	40.0	37.0	41.0	34.0	41.0
20	38.402143601714165	40.0	37.0	41.0	34.0	41.0
21	38.26951381402518	40.0	37.0	41.0	34.0	41.0
22	38.28825696492369	40.0	37.0	41.0	34.0	41.0
23	38.256679822881125	40.0	37.0	41.0	34.0	41.0
24	38.17535927156743	40.0	37.0	41.0	34.0	41.0
25	38.00061697597135	40.0	36.0	41.0	34.0	41.0
26	38.0450837929099	40.0	36.0	41.0	34.0	41.0
27	38.047455920741974	40.0	36.0	41.0	34.0	41.0
28	37.99445167095803	40.0	36.0	41.0	34.0	41.0
29	37.943777229354694	40.0	36.0	41.0	34.0	41.0
30	37.785780151637994	40.0	36.0	41.0	33.0	41.0
31	37.72869539651286	40.0	36.0	41.0	33.0	41.0
32	37.633177715807946	40.0	36.0	41.0	33.0	41.0
33	37.51259343733573	40.0	35.0	41.0	33.0	41.0
34	37.38919824306626	40.0	35.0	41.0	33.0	41.0
35	37.314717883839236	40.0	35.0	41.0	33.0	41.0
36	37.21273866056076	40.0	35.0	41.0	33.0	41.0
37	37.181119198866725	40.0	35.0	41.0	33.0	41.0
38	37.058289751516824	40.0	35.0	41.0	32.0	41.0
39	37.01128821019057	39.0	35.0	41.0	32.0	41.0
40	36.88632718881692	39.0	35.0	41.0	32.0	41.0
41	36.79370283586212	39.0	35.0	41.0	32.0	41.0
42	36.7145606329238	39.0	35.0	41.0	31.0	41.0
43	36.560216409333485	39.0	35.0	41.0	31.0	41.0
44	36.56370666690425	39.0	35.0	41.0	31.0	41.0
45	36.531024759223456	39.0	35.0	41.0	31.0	41.0
46	36.4334423249971	39.0	35.0	41.0	31.0	41.0
47	36.36693587904598	39.0	35.0	41.0	31.0	41.0
48	36.324620682281875	38.0	35.0	41.0	31.0	41.0
49	36.27916714925918	38.0	35.0	41.0	31.0	41.0
50	36.17283122922996	38.0	35.0	40.0	31.0	41.0
51	35.33524068744933	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	7.0
11	6.0
12	5.0
13	10.0
14	14.0
15	20.0
16	27.0
17	66.0
18	113.0
19	181.0
20	332.0
21	495.0
22	771.0
23	1095.0
24	1643.0
25	2455.0
26	3363.0
27	4134.0
28	4532.0
29	5062.0
30	5713.0
31	6983.0
32	8990.0
33	12800.0
34	25039.0
35	36061.0
36	33603.0
37	49874.0
38	87416.0
39	157998.0
40	150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.47868425976248	19.94591994012883	24.178553291577945	13.396842508530751
2	32.869450557282995	24.066517582701508	24.90088292157055	18.163148938444955
3	32.84405876640443	23.47404246220187	26.258898263557878	17.423000507835816
4	29.33531419000187	24.718017480243404	26.25756185351164	19.689106476243083
5	25.601829990823315	29.10344704697927	24.57413066526492	20.720592296932494
6	26.61260145579601	33.981789185769905	24.635160057376538	14.770449301057544
7	77.84633066348304	6.825268841154301	10.285011715861407	5.043388779501252
8	77.87194518936931	6.225443465400344	10.073636193547813	5.82897515168254
9	71.51063336926792	8.130718721322868	12.69010432907761	7.668543580331607
10	40.59456882957208	24.369659928190234	19.435188567457523	15.600582674780162
11	31.238807565862743	24.297939255708698	24.053598952254525	20.409654226174037
12	27.216658796696397	21.63714685364528	30.106868256697645	21.039326092960682
13	25.37419481294714	22.396227759909483	31.073538190144422	21.156039236998957
14	21.11951069573507	26.60079650038756	30.2498641316453	22.02982867223207
15	19.62919075917	24.427348295186253	34.113202840316816	21.83025810532693
16	22.245658894699798	22.738794201762282	32.70329024153384	22.31225666200408
17	22.4002369900482	22.909631952673266	28.710987963400182	25.97914309387835
18	22.81207401929776	24.02330699120642	30.045393394570613	23.119225594925204
19	25.137872969770402	24.8521039548828	27.36165928671341	22.64836378863339
20	26.390757388120207	24.281011395122995	27.255860158052762	22.07237105870404
21	24.771473882092995	25.296237560249818	27.81336588234246	22.118922675314725
22	23.573605010646734	23.875633681096925	26.962295417895422	25.58846589036092
23	23.318573426822642	25.421860104596362	27.297957074509316	23.961609394071683
24	23.316123341737867	24.202385937402553	28.01716841439403	24.46432230646555
25	23.80925864880035	24.7280405555902	26.346878591601996	25.115822204007447
26	22.706943095660233	25.382436008232283	27.713803333897598	24.196817562209887
27	23.931094698015876	24.47657273188942	27.315107670102723	24.27722489999198
28	21.863000151459804	25.41183702924956	27.707121283666396	25.018041535624235
29	23.763152502205077	24.393492574014843	26.915298331269323	24.928056592510757
30	23.90303008704484	24.244037383843693	26.76896143120607	25.0839710979054
31	24.52512896356946	25.563074099482364	23.915057777460998	25.996739159487177
32	24.962580518705284	24.987526839568428	25.073725287550896	24.97616735417539
33	23.668712858937464	24.570566905141614	25.92969592216748	25.831024313753442
34	22.753717447278625	25.0739480225586	26.10721572330966	26.06511880685311
35	23.503443483219144	23.029463386819433	26.878769790005435	26.588323339955988
36	23.813713348954483	25.157028180433176	27.040698140608153	23.988560330004187
37	22.67397831451965	26.37249311748826	26.452677720262653	24.50085084772944
38	21.658529414385118	26.121916233818304	26.73956041018879	25.47999394160779
39	23.416576830213558	23.6664855088604	28.190456250389783	24.72648141053626
40	22.56617457078964	23.9453497385091	30.911832574549408	22.57664311615185
41	21.142452401528853	24.640505697561498	28.448828859329478	25.768213041580175
42	22.53833269482631	24.228668668311936	28.503176201209897	24.729822435651858
43	23.080915173599664	23.03258167692733	28.469543215046194	25.416959934426814
44	22.567733715843584	24.028207161375967	27.294616049393717	26.10944307338673
45	23.07668320845324	23.135039780472376	26.90861628103812	26.87966073003626
46	23.545094929660284	24.385474113737406	27.818043317504298	24.251387639098013
47	22.07148011867321	24.283016010192355	29.451359129017025	24.19414474211741
48	21.590372502026888	23.366684188487273	28.842624352954804	26.20031895653104
49	22.651927548756692	22.553924145365777	29.93313495068647	24.861013355191062
50	21.02685293252911	22.94972425406046	29.38364768667421	26.63977512673622
51	21.145570691636745	23.238388824048254	27.596199249828494	28.01984123448651
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	290.0
1	605.5
2	921.0
3	2500.0
4	4079.0
5	2699.0
6	1319.0
7	1363.5
8	1408.0
9	1387.5
10	1367.0
11	1415.5
12	1464.0
13	1455.0
14	1446.0
15	1456.0
16	1466.0
17	1364.0
18	1262.0
19	1397.5
20	1533.0
21	1388.0
22	1243.0
23	1474.0
24	1705.0
25	1815.5
26	2388.0
27	2850.0
28	3705.5
29	4561.0
30	5297.0
31	6033.0
32	6523.5
33	7014.0
34	7818.5
35	8623.0
36	9304.0
37	9985.0
38	10912.0
39	11839.0
40	13419.5
41	15000.0
42	17514.0
43	20028.0
44	23152.5
45	26277.0
46	30470.0
47	34663.0
48	38806.5
49	42950.0
50	41582.5
51	40215.0
52	35293.0
53	30371.0
54	27592.0
55	24813.0
56	24113.5
57	23414.0
58	22597.5
59	21781.0
60	22406.0
61	23031.0
62	21009.5
63	18988.0
64	17284.5
65	15581.0
66	13314.5
67	11048.0
68	9903.5
69	8759.0
70	7688.0
71	6617.0
72	5831.0
73	5045.0
74	4244.0
75	2793.5
76	2144.0
77	1653.0
78	1162.0
79	899.5
80	637.0
81	501.0
82	365.0
83	298.5
84	232.0
85	136.5
86	41.0
87	23.5
88	6.0
89	5.5
90	5.0
91	5.5
92	6.0
93	5.5
94	5.0
95	4.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	448964.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.718398393769487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.7169203015243	23.064642480917318
2	9.148015116288954	5.803207759413572
3	3.3091155233994667	3.148795335065739
4	1.8704365600957285	2.373090079335523
5	1.2765524394060281	2.024509942180934
6	0.9609594645873234	1.8288057082826488
7	0.8553198995477512	1.899056412958213
8	0.743958590441787	1.8877739968079832
9	0.7056735863846826	2.014455235401852
>10	8.267146505951652	49.379076093982405
>50	0.08880992057444909	1.8739148064823161
>100	0.052863047960981596	3.1772938713584677
>500	0.0028193625579190184	0.6644326896761446
>1k	0.0014096812789595092	0.8609455881368885
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2530	0.5635195694977772	No Hit
GCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	1321	0.2942329451804599	Illumina PCR Primer Index 6 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGCT	862	0.19199757664311615	RNA PCR Primer, Index 27 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	794	0.17685159611906523	RNA PCR Primer, Index 27 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCC	792	0.17640612610365197	No Hit
TCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	524	0.11671314403827479	Illumina PCR Primer Index 6 (95% over 22bp)
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA	473	0.10535365864523659	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTC	449	0.10000801846027745	RNA PCR Primer, Index 27 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.682050231198938E-4	0.0	0.0	0.19845689186660845	0.0
2	6.682050231198938E-4	0.0	0.0	0.895171995972951	0.0
3	6.682050231198938E-4	0.0	0.0	1.252216213326681	0.0
4	6.682050231198938E-4	0.0	0.0	1.7466879304354024	0.0
5	6.682050231198938E-4	0.0	0.0	3.1577142042569113	0.0
6	6.682050231198938E-4	0.0	0.0	3.9767108275941947	0.0
7	6.682050231198938E-4	0.0	0.0	4.80350317620121	0.0
8	6.682050231198938E-4	0.0	0.0	6.120535276770521	0.0
9	6.682050231198938E-4	0.0	0.0	6.7114512522162135	0.0
10	6.682050231198938E-4	0.0	0.0	7.873237052414002	0.0
11	6.682050231198938E-4	0.0	0.0	9.29695922167479	0.0
12	6.682050231198938E-4	0.0	0.0	10.492823478051692	0.0
13	6.682050231198938E-4	0.0	0.0	10.945421013711567	0.0
14	6.682050231198938E-4	0.0	0.0	11.11314047451466	0.0
15	6.682050231198938E-4	0.0	0.0	11.484216997353908	0.0
16	6.682050231198938E-4	0.0	0.0	12.167345265990146	0.0
17	6.682050231198938E-4	0.0	0.0	12.989460179435323	0.0
18	6.682050231198938E-4	0.0	0.0	13.881736620308088	0.0
19	6.682050231198938E-4	0.0	0.0	14.364626117016064	0.0
20	6.682050231198938E-4	0.0	0.0	14.853083988916707	0.0
21	6.682050231198938E-4	0.0	0.0	15.448677399524238	0.0
22	8.90940030826525E-4	0.0	0.0	16.05830311561729	0.0
23	0.0011136750385331564	0.0	0.0	16.654787466255648	0.0
24	0.0011136750385331564	0.0	0.0	17.124758332516638	0.0
25	0.0011136750385331564	0.0	0.0	17.570673817945313	0.0
26	0.0011136750385331564	0.0	0.0	17.957341791324026	0.0
27	0.0011136750385331564	0.0	0.0	18.356705660142016	0.0
28	0.0011136750385331564	0.0	0.0	18.76186063916038	0.0
29	0.0011136750385331564	0.0	0.0	19.174588608440764	0.0
30	0.0011136750385331564	0.0	0.0	19.620058623854028	0.0
31	0.0011136750385331564	0.0	0.0	20.075328979606383	0.0
32	0.0011136750385331564	0.0	0.0	20.498302759241277	0.0
33	0.0011136750385331564	0.0	0.0	20.92439482898406	0.0
34	0.0013364100462397875	0.0	0.0	21.34113202840317	0.0
35	0.0013364100462397875	0.0	0.0	21.796179649147817	0.0
36	0.0013364100462397875	0.0	0.0	22.207571208381964	0.0
37	0.0013364100462397875	0.0	0.0	22.643686353471548	0.0
38	0.0013364100462397875	0.0	0.0	23.06487825304479	0.0
39	0.0013364100462397875	0.0	0.0	23.468251352001495	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTAG	25	3.8869952E-5	45.0	1
TCTAGCG	20	7.028689E-4	45.0	1
ATACTCG	20	7.028689E-4	45.0	44
CCCAACG	20	7.028689E-4	45.0	31
GTGCTCG	20	7.028689E-4	45.0	1
TACGTTG	25	3.8869952E-5	45.0	1
GTACAAC	30	2.1625347E-6	44.999996	14
GTCTTAC	65	0.0	41.538464	31
TCGTTCA	50	1.0786607E-9	40.5	16
CACGACG	50	1.0786607E-9	40.5	26
CGTTAGG	50	1.0786607E-9	40.5	2
TGTTGCG	50	1.0786607E-9	40.5	1
CTAGCGG	95	0.0	40.263157	2
TAGCGGG	270	0.0	40.000004	3
TTAGTCA	45	1.9237632E-8	40.000004	32
CACAACG	45	1.9237632E-8	40.000004	12
GCGTAAG	45	1.9237632E-8	40.000004	1
CGTTTTT	1210	0.0	39.79339	1
GTAGTCA	85	0.0	39.705883	9
TTCGGCA	40	3.4519508E-7	39.375	18
>>END_MODULE