FastQCFastQC Report
Sat 18 Jun 2016
SRR3552034_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552034_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences576366
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93251.6178955732989109No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTC58051.0071725257909037No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCG50030.868024831443909No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGC40910.7097920418622888No Hit
GCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC17000.2949514718078443RNA PCR Primer, Index 14 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCT14480.25122925363397564RNA PCR Primer, Index 14 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCT11500.1995259956347182No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCC10170.17645038048739864No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTC8520.1478227376354608No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTCCATAT8400.14574072724622897No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTCCATATCGTA6850.11884809305198432No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTT6620.11485757313928996No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT6020.10444752119313076No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT351.2105193E-745.028
TACGCGC207.030356E-445.034
AATTGCG207.030356E-445.01
TAAGACG351.2105193E-745.01
GTCGATG253.888378E-544.9999961
CGCACGG253.888378E-544.9999962
TACAGTC302.1636115E-644.99999645
TAATGCG502.1827873E-1144.9999961
CGTTTTT37300.042.828421
AATGCGG950.042.631582
CCCTCGT750.042.00000414
TGCGAAG556.002665E-1140.9090921
CGATGAA5900.040.80508419
TATACGG403.4542245E-739.3752
GATGAAT6300.038.92857420
GCGGGCA2200.038.8636365
CCTATCG356.2428717E-638.57142640
CGTTTAT356.2428717E-638.57142639
CCGATGA6250.038.5218
CGGGTAC659.094947E-1238.076926
Produced by FastQC (version 0.11.5)