##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552034_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	576366
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24672343614995	33.0	31.0	34.0	30.0	34.0
2	32.344862465863706	34.0	31.0	34.0	30.0	34.0
3	32.32479709073748	34.0	31.0	34.0	30.0	34.0
4	35.90535527772283	37.0	35.0	37.0	35.0	37.0
5	35.96282049947429	37.0	35.0	37.0	35.0	37.0
6	35.94597009539078	37.0	35.0	37.0	35.0	37.0
7	36.12383971296017	37.0	35.0	37.0	35.0	37.0
8	35.92970612423356	37.0	35.0	37.0	35.0	37.0
9	37.835077017034315	39.0	38.0	39.0	35.0	39.0
10	37.55465277271733	39.0	37.0	39.0	35.0	39.0
11	37.50830895646169	39.0	37.0	39.0	35.0	39.0
12	37.444804169572805	39.0	37.0	39.0	35.0	39.0
13	37.40579076489592	39.0	37.0	39.0	35.0	39.0
14	38.706440005135626	40.0	38.0	41.0	35.0	41.0
15	38.72053868548804	40.0	38.0	41.0	35.0	41.0
16	38.74697848242263	40.0	38.0	41.0	35.0	41.0
17	38.673577206150256	40.0	38.0	41.0	35.0	41.0
18	38.54376906340762	40.0	38.0	41.0	35.0	41.0
19	38.413258242158626	40.0	37.0	41.0	34.0	41.0
20	38.20382014206251	40.0	36.0	41.0	34.0	41.0
21	38.1806005211966	40.0	36.0	41.0	34.0	41.0
22	38.19482238716371	40.0	36.0	41.0	34.0	41.0
23	38.11656655666712	40.0	36.0	41.0	34.0	41.0
24	38.071699232779174	40.0	36.0	41.0	34.0	41.0
25	37.94092122019689	40.0	36.0	41.0	34.0	41.0
26	37.95084199970158	40.0	36.0	41.0	34.0	41.0
27	37.911020080990205	40.0	36.0	41.0	34.0	41.0
28	37.80653612461526	40.0	36.0	41.0	33.0	41.0
29	37.75980887144627	40.0	36.0	41.0	33.0	41.0
30	37.6101574346856	40.0	35.0	41.0	33.0	41.0
31	37.52867101806838	40.0	35.0	41.0	33.0	41.0
32	37.29359469503753	40.0	35.0	41.0	33.0	41.0
33	37.07427225061853	40.0	35.0	41.0	32.0	41.0
34	36.87003397146952	40.0	35.0	41.0	31.0	41.0
35	36.7142822442684	40.0	35.0	41.0	31.0	41.0
36	36.609860748205136	40.0	35.0	41.0	31.0	41.0
37	36.564007939399616	40.0	35.0	41.0	30.0	41.0
38	36.47353244292689	40.0	35.0	41.0	30.0	41.0
39	36.383650319415096	40.0	35.0	41.0	30.0	41.0
40	36.32363116491951	39.0	35.0	41.0	30.0	41.0
41	36.17738728516255	39.0	35.0	41.0	29.0	41.0
42	36.07181027333326	39.0	35.0	41.0	29.0	41.0
43	35.96540219235694	39.0	35.0	41.0	28.0	41.0
44	36.02620383575714	39.0	35.0	41.0	29.0	41.0
45	35.98755998792434	39.0	35.0	41.0	28.0	41.0
46	35.9023259526065	39.0	35.0	41.0	28.0	41.0
47	35.79880319102792	39.0	35.0	41.0	28.0	41.0
48	35.791311423644004	39.0	35.0	41.0	28.0	41.0
49	35.76926813864801	39.0	35.0	41.0	28.0	41.0
50	35.672621216379866	38.0	35.0	41.0	28.0	41.0
51	34.85773449509513	37.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	6.0
10	8.0
11	6.0
12	13.0
13	15.0
14	13.0
15	36.0
16	37.0
17	93.0
18	158.0
19	335.0
20	654.0
21	1183.0
22	1927.0
23	2651.0
24	3733.0
25	5032.0
26	7017.0
27	8175.0
28	8017.0
29	7559.0
30	8169.0
31	9358.0
32	11808.0
33	15719.0
34	27915.0
35	40481.0
36	40639.0
37	56637.0
38	103918.0
39	214770.0
40	282.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.313738145553344	18.41382038496372	23.04924301572265	16.223198453760286
2	34.578722547825514	27.15184448770399	22.091691737541773	16.17774122692872
3	28.89396668089374	26.37022308741321	29.439626903738247	15.296183327954807
4	26.31782582595087	23.524461887064817	33.05035342126357	17.10735886572074
5	26.90859627389541	26.8950632063654	27.022933344437387	19.173407175301804
6	24.325168382590228	35.39417661694132	26.663613051429124	13.617041949039328
7	73.65736355024411	5.912042001089586	16.13471301221793	4.295881436448368
8	74.04583198870162	8.406290447389333	13.003022385081703	4.544855178827342
9	67.58500674918368	8.150203169513816	14.591249310334058	9.673540770968447
10	36.12912628434016	28.299379213902277	21.27988118660712	14.291613315150443
11	28.27387458663419	22.828549914464073	29.708379744814927	19.189195754086814
12	24.578306145747668	20.531398451678275	33.6626379765635	21.227657426010555
13	24.08972770774126	21.9060458111686	35.47433401692675	18.529892464163396
14	20.847170027378436	27.141434435757834	31.487631123279304	20.523764413584423
15	19.04154651731712	23.86157406925461	36.83093728637706	20.265942127051215
16	20.934093961128866	23.509367311742885	32.7363168542211	22.820221872907144
17	20.48368571359171	24.610750807646532	32.17573555692043	22.72982792184133
18	20.79806928236572	24.21725084408171	32.48578160405021	22.498898269502366
19	21.51792437444263	25.1973572348126	30.59601017409077	22.688708216654003
20	23.21944736504235	25.015007824889047	31.847818920616415	19.91772588945219
21	22.396879760430004	26.817334818500743	30.99332715670251	19.79245826436674
22	21.19608026844054	22.926404402757967	31.4282938271862	24.44922150161529
23	21.44314550129605	25.49213520575468	31.337205872657304	21.727513420291967
24	21.07324165547586	25.562229555525484	31.075393066211397	22.289135722787258
25	21.047737028207774	28.4400884160412	28.968919054906085	21.54325550084495
26	20.576855678509837	25.53811293518355	30.72283930696814	23.162192079338475
27	23.353216532550498	24.66956760114233	29.866959536128086	22.110256330179087
28	20.13633698032153	25.11685283309564	32.26769101577817	22.479119170804662
29	22.53810946516623	23.197586255955418	30.671309549834653	23.5929947290437
30	21.227657426010555	25.33633142829383	30.38538012304681	23.050631022648805
31	22.45916657124119	24.889740199803597	28.717516300406338	23.933576928548874
32	23.264731091008144	25.27577962614033	27.807504259446254	23.651985023405267
33	21.79812827266008	24.797437739214317	27.033343396383547	26.371090591742053
34	21.838727475250103	26.138599431611166	28.09152517671063	23.931147916428102
35	21.943174996443233	24.190358209887467	28.807736750606384	25.058730043062916
36	21.8319609414851	27.65759951142156	28.210893772359924	22.299545774733414
37	20.166526130965394	26.62440185576526	29.885003626168093	23.324068387101253
38	19.7745876751925	26.15699052338271	29.000322711610334	25.06809908981446
39	21.58697771901882	26.45367700384825	27.93658890357863	24.022756373554305
40	20.805876821325338	25.13350891620949	30.66731902992196	23.39329523254321
41	19.8203919037556	28.105405245972175	28.68455113591017	23.389651714362056
42	20.50658782787326	25.866897075816407	31.1211972947745	22.505317801535828
43	21.809926331532395	25.384911670709236	28.848335953196408	23.956826044561964
44	21.932764944497073	24.484095175634927	29.25762449554623	24.325515384321765
45	21.125812417803967	23.941731469240032	29.279832606364707	25.652623506591297
46	23.152129029123856	25.908710784466816	28.415451293101953	22.52370889330738
47	19.96873514398837	25.712654806147484	32.005704708466496	22.312905341397656
48	20.757990582373008	25.194060718362987	29.614168774702186	24.433779924561826
49	20.33169895517779	24.282313668745207	31.99217164093649	23.393815735140517
50	20.235232473810044	23.764413584423785	31.367221522435397	24.63313241933077
51	20.35616257725126	23.968624103434276	29.173303074782343	26.501910244532116
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	490.0
1	764.5
2	1039.0
3	7166.5
4	13294.0
5	8394.5
6	3495.0
7	3431.0
8	3367.0
9	3458.0
10	3549.0
11	3487.5
12	3426.0
13	3373.0
14	3320.0
15	3068.5
16	2817.0
17	2637.5
18	2458.0
19	2376.5
20	2295.0
21	2366.0
22	2437.0
23	2708.5
24	2980.0
25	3319.5
26	4465.5
27	5272.0
28	6209.0
29	7146.0
30	8176.0
31	9206.0
32	10349.5
33	11493.0
34	12453.5
35	13414.0
36	14424.0
37	15434.0
38	16819.0
39	18204.0
40	20406.0
41	22608.0
42	24816.5
43	27025.0
44	30090.0
45	33155.0
46	47246.0
47	61337.0
48	55135.5
49	48934.0
50	48157.0
51	47380.0
52	42599.5
53	37819.0
54	34660.0
55	31501.0
56	29560.5
57	27620.0
58	25963.5
59	24307.0
60	23381.0
61	22455.0
62	20425.0
63	18395.0
64	16064.0
65	13733.0
66	11647.5
67	9562.0
68	8120.5
69	6679.0
70	6004.5
71	5330.0
72	4515.5
73	3701.0
74	3141.5
75	2081.5
76	1581.0
77	1280.5
78	980.0
79	721.5
80	463.0
81	362.0
82	261.0
83	186.5
84	112.0
85	75.5
86	39.0
87	23.0
88	7.0
89	5.0
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	576366.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.813792086875452
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.30449870572797	23.3209408085666
2	7.996904808935135	5.088237338599937
3	3.1871879713921123	3.0418960639097703
4	1.955993292906841	2.489102557754445
5	1.4155604204061156	2.251717245060508
6	1.2119566091132632	2.3134161348386364
7	1.043628260307552	2.3241240742588394
8	0.9334483343227473	2.3757224985587277
9	0.8793812066111462	2.51788057850094
>10	7.957793290151792	44.22050708258059
>50	0.06698041649902417	1.4993159479559954
>100	0.03843138651583226	2.564242478091965
>500	0.003843138651583225	0.8334967035934103
>1k	0.002745099036845161	1.642889772422384
>5k	0.0016470594221070967	3.516510715307236
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9325	1.6178955732989109	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTC	5805	1.0071725257909037	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCG	5003	0.868024831443909	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGC	4091	0.7097920418622888	No Hit
GCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCTGC	1700	0.2949514718078443	RNA PCR Primer, Index 14 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTTCT	1448	0.25122925363397564	RNA PCR Primer, Index 14 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCT	1150	0.1995259956347182	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCC	1017	0.17645038048739864	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTC	852	0.1478227376354608	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTCCATAT	840	0.14574072724622897	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTCCATATCGTA	685	0.11884809305198432	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCTCCATATCGTATGCCGTCTT	662	0.11485757313928996	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	602	0.10444752119313076	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.205025973079605E-4	0.0	0.0	0.09837499089120455	0.0
2	5.205025973079605E-4	0.0	0.0	0.5860859245687636	0.0
3	5.205025973079605E-4	0.0	0.0	0.8411321972496643	0.0
4	5.205025973079605E-4	0.0	0.0	1.3965084685772582	0.0
5	5.205025973079605E-4	0.0	0.0	3.2604282695370648	0.0
6	5.205025973079605E-4	0.0	0.0	4.063910778914787	0.0
7	5.205025973079605E-4	0.0	0.0	4.860279752795966	0.0
8	5.205025973079605E-4	0.0	0.0	5.930086091129595	0.0
9	5.205025973079605E-4	0.0	0.0	6.394200907062526	0.0
10	5.205025973079605E-4	0.0	0.0	8.09815290978302	0.0
11	5.205025973079605E-4	0.0	0.0	9.142801622580096	0.0
12	5.205025973079605E-4	0.0	0.0	10.680192794162043	0.0
13	5.205025973079605E-4	0.0	0.0	11.078724282834171	0.0
14	5.205025973079605E-4	0.0	0.0	11.25552166505311	0.0
15	5.205025973079605E-4	0.0	0.0	11.622128994423683	0.0
16	5.205025973079605E-4	0.0	0.0	12.080518281786226	0.0
17	5.205025973079605E-4	0.0	0.0	12.619585471731504	0.0
18	5.205025973079605E-4	0.0	0.0	13.229094013179125	0.0
19	5.205025973079605E-4	0.0	0.0	13.720968967635148	0.0
20	5.205025973079605E-4	0.0	0.0	14.107875898300733	0.0
21	6.940034630772807E-4	0.0	0.0	14.500681858402473	0.0
22	6.940034630772807E-4	0.0	0.0	14.96618468126156	0.0
23	8.675043288466009E-4	0.0	0.0	15.385883275557545	0.0
24	8.675043288466009E-4	0.0	0.0	15.727159478525797	0.0
25	8.675043288466009E-4	0.0	0.0	16.049003584527888	0.0
26	8.675043288466009E-4	0.0	0.0	16.35957013425497	0.0
27	8.675043288466009E-4	0.0	0.0	16.675341709955134	0.0
28	8.675043288466009E-4	0.0	0.0	16.99232779171568	0.0
29	8.675043288466009E-4	0.0	0.0	17.303067842308533	0.0
30	8.675043288466009E-4	0.0	0.0	17.66637865522949	0.0
31	8.675043288466009E-4	0.0	0.0	17.986487752573886	0.0
32	8.675043288466009E-4	0.0	0.0	18.288726260744042	0.0
33	8.675043288466009E-4	0.0	0.0	18.59252627670612	0.0
34	8.675043288466009E-4	0.0	0.0	18.91176786972167	0.0
35	8.675043288466009E-4	0.0	0.0	19.259637105589157	0.0
36	8.675043288466009E-4	0.0	0.0	19.579225700336245	0.0
37	8.675043288466009E-4	0.0	0.0	19.898814295083334	0.0
38	8.675043288466009E-4	0.0	0.0	20.190989753038867	0.0
39	8.675043288466009E-4	0.0	0.0	20.514221865967112	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	35	1.2105193E-7	45.0	28
TACGCGC	20	7.030356E-4	45.0	34
AATTGCG	20	7.030356E-4	45.0	1
TAAGACG	35	1.2105193E-7	45.0	1
GTCGATG	25	3.888378E-5	44.999996	1
CGCACGG	25	3.888378E-5	44.999996	2
TACAGTC	30	2.1636115E-6	44.999996	45
TAATGCG	50	2.1827873E-11	44.999996	1
CGTTTTT	3730	0.0	42.82842	1
AATGCGG	95	0.0	42.63158	2
CCCTCGT	75	0.0	42.000004	14
TGCGAAG	55	6.002665E-11	40.909092	1
CGATGAA	590	0.0	40.805084	19
TATACGG	40	3.4542245E-7	39.375	2
GATGAAT	630	0.0	38.928574	20
GCGGGCA	220	0.0	38.863636	5
CCTATCG	35	6.2428717E-6	38.571426	40
CGTTTAT	35	6.2428717E-6	38.571426	39
CCGATGA	625	0.0	38.52	18
CGGGTAC	65	9.094947E-12	38.07692	6
>>END_MODULE