##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552028_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	409274
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.283570419816556	33.0	31.0	34.0	30.0	34.0
2	32.374951743819544	34.0	31.0	34.0	30.0	34.0
3	32.376923528003246	34.0	31.0	34.0	30.0	34.0
4	35.93377297360692	37.0	35.0	37.0	35.0	37.0
5	35.99936472876362	37.0	35.0	37.0	35.0	37.0
6	35.974298391786434	37.0	35.0	37.0	35.0	37.0
7	36.14642024658298	37.0	36.0	37.0	35.0	37.0
8	35.97648763420105	37.0	35.0	37.0	35.0	37.0
9	37.88019517487062	39.0	38.0	39.0	35.0	39.0
10	37.56648602158945	39.0	37.0	39.0	35.0	39.0
11	37.54021755596495	39.0	37.0	39.0	35.0	39.0
12	37.471903907895445	39.0	37.0	39.0	35.0	39.0
13	37.438913783919816	39.0	37.0	39.0	35.0	39.0
14	38.69734700958282	40.0	38.0	41.0	35.0	41.0
15	38.75708938266296	40.0	38.0	41.0	35.0	41.0
16	38.748642718569954	40.0	38.0	41.0	35.0	41.0
17	38.686154019067914	40.0	38.0	41.0	35.0	41.0
18	38.616418340769265	40.0	38.0	41.0	35.0	41.0
19	38.54743032784882	40.0	38.0	41.0	35.0	41.0
20	38.480721961326644	40.0	37.0	41.0	34.0	41.0
21	38.37348084657222	40.0	37.0	41.0	34.0	41.0
22	38.40502450680962	40.0	37.0	41.0	34.0	41.0
23	38.364240093433736	40.0	37.0	41.0	34.0	41.0
24	38.283795208100194	40.0	37.0	41.0	34.0	41.0
25	38.10306298469974	40.0	37.0	41.0	34.0	41.0
26	38.127953400411464	40.0	37.0	41.0	34.0	41.0
27	38.12928747000787	40.0	37.0	41.0	34.0	41.0
28	38.05640719908912	40.0	36.0	41.0	34.0	41.0
29	38.01293754306406	40.0	36.0	41.0	34.0	41.0
30	37.85822456349536	40.0	36.0	41.0	33.0	41.0
31	37.840346564892954	40.0	36.0	41.0	33.0	41.0
32	37.72705326993652	40.0	36.0	41.0	33.0	41.0
33	37.652812052561366	40.0	36.0	41.0	33.0	41.0
34	37.530954812668284	40.0	36.0	41.0	33.0	41.0
35	37.40281816093864	40.0	35.0	41.0	33.0	41.0
36	37.32045280179049	40.0	35.0	41.0	33.0	41.0
37	37.246724688106255	40.0	35.0	41.0	33.0	41.0
38	37.13732609449904	40.0	35.0	41.0	32.0	41.0
39	37.09282045768849	40.0	35.0	41.0	32.0	41.0
40	36.9739978596246	39.0	35.0	41.0	32.0	41.0
41	36.89382662959289	39.0	35.0	41.0	32.0	41.0
42	36.76724639239238	39.0	35.0	41.0	32.0	41.0
43	36.652210988237705	39.0	35.0	41.0	31.0	41.0
44	36.64879029696487	39.0	35.0	41.0	31.0	41.0
45	36.61601518786925	39.0	35.0	41.0	31.0	41.0
46	36.53419713932475	39.0	35.0	41.0	31.0	41.0
47	36.46092593226054	39.0	35.0	41.0	31.0	41.0
48	36.392663594560126	39.0	35.0	41.0	31.0	41.0
49	36.324845946725176	38.0	35.0	41.0	31.0	41.0
50	36.23125827685121	38.0	35.0	41.0	31.0	41.0
51	35.37281625512493	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	6.0
10	12.0
11	9.0
12	9.0
13	6.0
14	21.0
15	25.0
16	25.0
17	49.0
18	101.0
19	172.0
20	294.0
21	527.0
22	751.0
23	1006.0
24	1512.0
25	2155.0
26	3092.0
27	3647.0
28	3893.0
29	4378.0
30	5088.0
31	6203.0
32	8177.0
33	11094.0
34	21845.0
35	31201.0
36	29473.0
37	44807.0
38	78020.0
39	151459.0
40	216.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.23802635886961	20.74698123995172	24.082155230969963	11.932837170208712
2	33.97381705165732	25.322644487556012	23.447616999858287	17.255921460928374
3	32.691057824342614	24.607964346623532	26.335657774498255	16.365320054535594
4	29.524963716239	25.418912513377347	26.087657657217417	18.96846611316624
5	26.124063585764063	29.095666961497674	23.808988599324657	20.971280853413607
6	26.68774464050978	34.79893665368433	23.65114813059222	14.862170575213671
7	78.05528814437271	7.097934391141387	9.805900203775465	5.040877260710428
8	78.14764680873938	7.205197496053989	9.39859360721668	5.248562087989953
9	71.47827616706655	9.03209097084105	11.616667562562	7.872965299530388
10	39.425177265108466	25.697210181931908	18.866578380253817	16.011034172705816
11	30.411411426086193	24.461363292073283	24.289107052976735	20.83811822886379
12	26.943807815790887	22.51108059637309	29.724829820609177	20.820281767226845
13	24.875755606268662	23.722982647321842	31.051080694107124	20.350181052302368
14	21.400333273064014	26.603693369234303	29.661302696970733	22.334670660730954
15	21.775876307803575	24.88748369063268	32.386371965969005	20.950268035594735
16	23.156369571485115	24.34066175716024	30.193708860079067	22.309259811275574
17	23.375782483128663	24.53955052116675	28.060663516372898	24.024003479331697
18	23.924314762237522	24.69861266535377	28.845712163489495	22.531360408919205
19	24.67931019317132	25.554029818654495	26.872950639424932	22.89370934874925
20	25.962558090667866	24.70203335662661	27.791161911091343	21.544246641614176
21	25.40278639737682	26.275307007041736	27.209155724526845	21.1127508710546
22	24.00665568787658	24.1141631278801	27.13854288325181	24.740638300991513
23	24.433020421526898	25.341946959738465	27.643339181086507	22.581693437648127
24	24.01374140551318	24.543948552803258	28.455509023294905	22.986801018388658
25	22.872696530930376	26.316599637406725	27.184722215435137	23.625981616227758
26	22.729027497471133	25.955228037940355	27.63894114945	23.676803315138514
27	24.373891329524962	25.83696985393648	27.13829854816089	22.650840268377664
28	22.028274456720926	27.110444347796342	28.033542321281097	22.827738874201636
29	24.25416713497559	25.967933462668043	26.445852900501865	23.332046501854503
30	23.5536584293163	26.960178266882334	26.741987030693377	22.744176273107993
31	25.097123198639544	26.62885988359876	24.19064001133715	24.083376906424547
32	24.926821640270333	27.791406246182266	25.094924182821288	22.186847930726113
33	23.959499015329584	27.070373392885937	25.088327135366526	23.881800456417952
34	23.503569735678298	26.869529948152092	25.94349995357633	23.683400362593275
35	24.67026979480739	27.183256204889634	25.46387017010609	22.682603830196886
36	25.106896602276223	28.138606410375445	24.882352653723423	21.872144333624906
37	23.949481276601983	29.33707003132376	25.205363643915813	21.508085048158446
38	22.509370250736673	29.014059041131368	25.372000175921265	23.104570532210694
39	25.325087838465183	26.040012314488582	26.328816391952582	22.306083455093653
40	24.467471669346207	25.852607299755174	27.605955912176196	22.073965118722423
41	22.571920034011445	27.293695665984156	26.055161090125438	24.079223209878954
42	22.72756148692563	27.448604113625592	26.311468600497466	23.512365798951315
43	24.696657984626434	24.79634670172061	26.757135806330233	23.749859507322725
44	23.42318349076658	25.752185577388254	26.451228272502043	24.373402659343128
45	23.634044674228022	25.322400152465097	26.025107873942638	25.018447299364237
46	23.61352052659099	27.242140961800654	25.92688516739397	23.21745334421439
47	23.30736865767188	26.170487253038306	27.97514623455191	22.546997854737903
48	22.551884556556242	25.745588529933492	27.591784476903	24.11074243660726
49	22.573386044556948	25.590924417382976	28.915347664400866	22.92034187365921
50	22.00579562835655	25.16602569427816	28.860372268944523	23.967806408420763
51	22.12161046145125	25.311893743555665	26.87417231487952	25.692323480113565
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	254.0
1	597.0
2	940.0
3	2129.5
4	3319.0
5	2253.5
6	1188.0
7	1164.5
8	1141.0
9	1139.0
10	1137.0
11	1158.0
12	1179.0
13	1200.5
14	1222.0
15	1155.5
16	1089.0
17	1134.5
18	1180.0
19	1186.0
20	1192.0
21	1233.0
22	1274.0
23	1450.0
24	1626.0
25	2147.0
26	3131.5
27	3595.0
28	4298.5
29	5002.0
30	5798.0
31	6594.0
32	7036.0
33	7478.0
34	8917.0
35	10356.0
36	10643.0
37	10930.0
38	11879.5
39	12829.0
40	14174.5
41	15520.0
42	17455.5
43	19391.0
44	21568.0
45	23745.0
46	27840.0
47	31935.0
48	33709.0
49	35483.0
50	34760.5
51	34038.0
52	31018.0
53	27998.0
54	25654.0
55	23310.0
56	22705.0
57	22100.0
58	21176.5
59	20253.0
60	19587.0
61	18921.0
62	17345.5
63	15770.0
64	14702.0
65	13634.0
66	11555.0
67	9476.0
68	8342.5
69	7209.0
70	6136.0
71	5063.0
72	4534.0
73	4005.0
74	3156.0
75	1776.5
76	1246.0
77	1057.0
78	868.0
79	683.0
80	498.0
81	333.0
82	168.0
83	136.0
84	104.0
85	67.5
86	31.0
87	17.5
88	4.0
89	3.0
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	409274.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.296856514544295
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.385682886522	22.341474744922394
2	7.901246428551005	4.945683515208328
3	3.1461494188711865	2.9539376080718536
4	1.9937507653685818	2.495925265180135
5	1.6300742799416081	2.550810042369081
6	1.335170699468968	2.5071987482222426
7	1.159822880507379	2.5409167191467423
8	1.0758316396679986	2.693611876839702
9	1.020160098747837	2.873502380913696
>10	9.210293627449788	47.76934936093107
>50	0.08226968986121494	1.7510974391741103
>100	0.05406293905165553	3.0625200837201323
>500	0.004701125134926568	1.0613008985780072
>1k	7.835208558210948E-4	0.452671316722505
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1846	0.4510425778329432	No Hit
GCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC	961	0.23480602237132092	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCG	787	0.19229171655174773	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTC	726	0.1773872760058054	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGC	645	0.15759613364152134	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	615	0.1502660809140087	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCC	594	0.14513504400474989	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	488	0.11923552436753862	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	457	0.1116611365491089	No Hit
CTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGCT	431	0.10530842418526463	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	424	0.10359807854884502	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10872911545810386	0.0
2	0.0	0.0	0.0	0.5829835269281703	0.0
3	0.0	0.0	0.0	0.81705654402674	0.0
4	0.0	0.0	0.0	1.174763117129356	0.0
5	0.0	0.0	0.0	2.300659216075294	0.0
6	0.0	0.0	0.0	2.9344644419142187	0.0
7	0.0	0.0	0.0	3.5465238446615226	0.0
8	0.0	0.0	0.0	4.455694717964005	0.0
9	0.0	0.0	0.0	4.83856780543108	0.0
10	0.0	0.0	0.0	5.812731812917508	0.0
11	0.0	0.0	0.0	6.872657437315833	0.0
12	0.0	0.0	0.0	7.778651954436392	0.0
13	0.0	0.0	0.0	8.09921959371961	0.0
14	2.443350909170873E-4	0.0	0.0	8.240445276269687	0.0
15	2.443350909170873E-4	0.0	0.0	8.54219911355229	0.0
16	2.443350909170873E-4	0.0	0.0	9.062144187023852	0.0
17	2.443350909170873E-4	0.0	0.0	9.662475505407135	0.0
18	2.443350909170873E-4	0.0	0.0	10.297013736518812	0.0
19	2.443350909170873E-4	0.0	0.0	10.680131159076804	0.0
20	2.443350909170873E-4	0.0	0.0	11.05298650781628	0.0
21	4.886701818341746E-4	0.0	0.0	11.487169964375944	0.0
22	4.886701818341746E-4	0.0	0.0	11.929416478935872	0.0
23	4.886701818341746E-4	0.0	0.0	12.373129004041303	0.0
24	4.886701818341746E-4	0.0	0.0	12.745984352780777	0.0
25	4.886701818341746E-4	0.0	0.0	13.089275155519285	0.0
26	4.886701818341746E-4	0.0	0.0	13.389562982256386	0.0
27	4.886701818341746E-4	0.0	0.0	13.681054745720472	0.0
28	4.886701818341746E-4	0.0	0.0	14.011151453549456	0.0
29	4.886701818341746E-4	0.0	0.0	14.332452098105426	0.0
30	4.886701818341746E-4	0.0	0.0	14.701642420481145	0.0
31	4.886701818341746E-4	0.0	0.0	15.053729286492667	0.0
32	4.886701818341746E-4	0.0	0.0	15.391644717231	0.0
33	4.886701818341746E-4	0.0	0.0	15.728582807605664	0.0
34	4.886701818341746E-4	0.0	0.0	16.06429922252574	0.0
35	4.886701818341746E-4	0.0	0.0	16.408078695446083	0.0
36	4.886701818341746E-4	0.0	0.0	16.757477875457518	0.0
37	4.886701818341746E-4	0.0	0.0	17.092705620195762	0.0
38	4.886701818341746E-4	0.0	0.0	17.42255799293383	0.0
39	4.886701818341746E-4	0.0	0.0	17.73775026021687	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATGGGC	140	0.0	45.000004	4
TACGGTT	35	1.2093733E-7	45.000004	33
ACGGTTA	35	1.2093733E-7	45.000004	34
ACCGAGC	20	7.0279575E-4	45.0	33
TAACCGG	20	7.0279575E-4	45.0	2
TATAGCG	20	7.0279575E-4	45.0	1
ATCGCAT	20	7.0279575E-4	45.0	10
GTACGAG	20	7.0279575E-4	45.0	1
AACACGT	55	1.8189894E-12	45.0	41
CGTAAGG	30	2.1620635E-6	44.999996	2
TACGAAT	80	0.0	42.1875	12
CGAATAT	80	0.0	42.1875	14
ACACGTG	60	3.6379788E-12	41.249996	42
TTAGGGT	115	0.0	41.08696	4
CACGACC	55	6.002665E-11	40.909092	27
CGAAAGG	50	1.0786607E-9	40.5	2
TATGGGT	50	1.0786607E-9	40.5	4
ATAGCGG	50	1.0786607E-9	40.5	2
CTATGCT	85	0.0	39.705883	36
CTCGTAC	40	3.4509503E-7	39.375	29
>>END_MODULE