FastQCFastQC Report
Sat 18 Jun 2016
SRR3552027_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552027_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences446256
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTC103692.323554193108888No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCG96492.1622118246029185No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGC69881.5659173209996058No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54851.229115126743394No Hit
GCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC35230.7894571725646267Illumina PCR Primer Index 11 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCT22990.5151751461044782Illumina PCR Primer Index 11 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCT22980.5149510594815532No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTC15720.3522641712380338No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTAACACCT13920.31192857911154137No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTAACACCTCGTA12650.2834695780000717No Hit
CTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGCT12040.2698002940016493Illumina PCR Primer Index 11 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC10620.23797999354630528Illumina PCR Primer Index 11 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCC10010.22431070954788282No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTT7580.16985766017711806No Hit
TCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC6670.1494657774909469Illumina PCR Primer Index 11 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG207.028642E-445.00000421
TAGTGCG207.028642E-445.0000041
TTACCAC207.028642E-445.00000439
CGATACG207.028642E-445.00000410
CCCATCG207.028642E-445.00000440
GTGCCTT207.028642E-445.00000434
TCACGAC253.8869577E-545.025
ACAACGA351.2097007E-745.013
AACCGAT351.2097007E-745.016
TAGGTCG253.8869577E-545.01
CGTTCAT502.1827873E-1145.017
CCATTAC453.8380676E-1045.06
CGAACGG302.1625056E-644.9999962
CGAACCC302.1625056E-644.99999634
GGCCGAT600.044.9999968
CGATGAA8400.043.66071319
CCGATGA8250.042.018
CGTTTTT22600.041.814161
GATGAAT9150.041.8032820
TACGGCT7700.041.4935077
Produced by FastQC (version 0.11.5)