##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552027_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	446256
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03389086085117	33.0	31.0	34.0	30.0	34.0
2	32.11556595317486	33.0	31.0	34.0	30.0	34.0
3	32.12250815675308	33.0	31.0	34.0	30.0	34.0
4	35.772534150801334	37.0	35.0	37.0	35.0	37.0
5	35.80248332795525	37.0	35.0	37.0	35.0	37.0
6	35.76301719192571	37.0	35.0	37.0	35.0	37.0
7	35.93855544799398	37.0	35.0	37.0	35.0	37.0
8	35.6393572299308	37.0	35.0	37.0	33.0	37.0
9	37.553733731311176	39.0	37.0	39.0	35.0	39.0
10	37.33180506256498	39.0	37.0	39.0	34.0	39.0
11	37.28972831737837	39.0	37.0	39.0	34.0	39.0
12	37.2160688035567	39.0	37.0	39.0	34.0	39.0
13	37.123247642608725	39.0	37.0	39.0	33.0	39.0
14	38.31793410060593	40.0	38.0	41.0	34.0	41.0
15	38.28476703954681	40.0	38.0	41.0	33.0	41.0
16	38.40985667419598	40.0	38.0	41.0	34.0	41.0
17	38.28138557240687	40.0	37.0	41.0	33.0	41.0
18	38.17396516797533	40.0	37.0	41.0	34.0	41.0
19	38.10642321895952	40.0	37.0	41.0	34.0	41.0
20	37.81611003549532	40.0	36.0	41.0	33.0	41.0
21	37.87721621670073	40.0	36.0	41.0	33.0	41.0
22	37.87264933132552	40.0	36.0	41.0	33.0	41.0
23	37.77126806138181	40.0	36.0	41.0	33.0	41.0
24	37.7399788462228	40.0	36.0	41.0	33.0	41.0
25	37.684497239252806	39.0	35.0	41.0	33.0	41.0
26	37.65907013014951	40.0	35.0	41.0	33.0	41.0
27	37.572729554336526	40.0	35.0	41.0	33.0	41.0
28	37.483092664300315	40.0	35.0	41.0	33.0	41.0
29	37.44388646875336	40.0	35.0	41.0	33.0	41.0
30	37.315507242479654	39.0	35.0	41.0	33.0	41.0
31	37.197418073930656	39.0	35.0	41.0	32.0	41.0
32	36.928083880104694	39.0	35.0	41.0	31.0	41.0
33	36.75379602739235	39.0	35.0	41.0	31.0	41.0
34	36.602013194220355	39.0	35.0	41.0	30.0	41.0
35	36.45862464594313	39.0	35.0	41.0	30.0	41.0
36	36.42183186332509	39.0	35.0	41.0	30.0	41.0
37	36.28533173783658	39.0	35.0	41.0	30.0	41.0
38	36.24365834857122	39.0	35.0	41.0	30.0	41.0
39	36.174563479258545	39.0	35.0	41.0	29.0	41.0
40	36.150303861460685	39.0	35.0	41.0	29.0	41.0
41	35.97256059302284	39.0	35.0	41.0	28.0	41.0
42	35.89279471872647	39.0	35.0	41.0	28.0	41.0
43	35.78516815460184	39.0	35.0	41.0	28.0	41.0
44	35.93099028360403	39.0	35.0	41.0	29.0	41.0
45	35.889679914667816	39.0	35.0	41.0	28.0	41.0
46	35.849247965293465	39.0	35.0	41.0	29.0	41.0
47	35.65644607579506	38.0	35.0	40.0	28.0	41.0
48	35.69035710444229	38.0	35.0	40.0	28.0	41.0
49	35.71883851421605	39.0	35.0	40.0	28.0	41.0
50	35.59210632462084	38.0	35.0	40.0	28.0	41.0
51	34.83994388870962	37.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	4.0
11	8.0
12	6.0
13	14.0
14	15.0
15	25.0
16	37.0
17	69.0
18	117.0
19	324.0
20	722.0
21	1269.0
22	1988.0
23	2651.0
24	3420.0
25	4449.0
26	5352.0
27	5718.0
28	5594.0
29	5803.0
30	6535.0
31	8179.0
32	10570.0
33	14512.0
34	24882.0
35	33558.0
36	33321.0
37	47598.0
38	83938.0
39	145442.0
40	126.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.36419454304256	18.501712021799147	21.0000537807895	14.134039654368793
2	31.644840629593773	32.48359685920189	20.62627729375067	15.245285217453658
3	27.904162633107454	31.492461726004805	25.67674160123337	14.926634039654369
4	25.5378078950199	21.820658993940697	35.70865153633789	16.932881574701515
5	30.19455200602345	24.997983220393674	24.58588792083468	20.2215768527482
6	22.414936717937685	36.92499372557456	26.483005270517374	14.177064285970383
7	67.8628410598401	6.051459252088487	20.707844824495357	5.377854863576064
8	65.30421999928292	12.841508013337636	15.114418629665483	6.739853357713958
9	59.845021691585096	7.661745724427235	15.928525330751855	16.56470725323581
10	37.67971747158582	22.40597325302069	23.387472661432003	16.526836613961493
11	29.25025097701768	21.45024380624574	28.113459538919365	21.186045677817216
12	24.80885411064501	18.82910257789251	33.31675092323689	23.04529238822559
13	24.372557455810117	21.045319278620344	37.30840593739916	17.273717328170378
14	19.035486357606395	29.502796601054104	29.986375533326164	21.475341508013337
15	16.91629916460507	22.81470725323581	39.17347890000358	21.09551468215553
16	18.73633071600158	24.51597289448209	30.224579613495393	26.523116776020938
17	18.959072819188986	25.812089921480048	31.16820838263239	24.060628876698576
18	19.333745652719514	25.37377648703883	31.278234914488547	24.01424294575311
19	19.930264242945753	25.775339715320357	28.35435265856369	25.9400433831702
20	22.27421031874081	26.43594707970313	32.25816571653938	19.031676885016672
21	20.45978093291743	30.120379333835288	30.148390161700906	19.271449571546377
22	18.649833279552546	23.581531676885017	30.864571008569076	26.904064034993368
23	20.732494353017103	27.394813739199027	30.450683016026677	21.422008891757198
24	21.919481194650604	25.58889964504679	28.121974830590514	24.36964432971209
25	19.028091499049875	31.769208705317126	26.727035602882648	22.47566419275035
26	19.026074719443546	23.813909504858195	30.77314366641569	26.386872109282567
27	24.205612921731024	24.672161270660787	28.089258183643466	23.03296762396472
28	17.611191782295364	26.79067620379334	30.89325409630347	24.70487791760783
29	20.676472697285863	23.588702448818616	28.433231149833276	27.301593704062242
30	19.142151590118676	28.175307446846652	30.5802050840773	22.10233587895737
31	20.78291384317522	29.282295364095944	24.23967408841562	25.69511670431322
32	22.842045821232656	29.77797497400595	23.95598042379262	23.423998780968773
33	19.848024452332293	25.7686171166326	23.966512495070095	30.416845935965007
34	18.780027607471943	28.580456061094978	24.419615646624358	28.219900684808717
35	19.13139543221828	24.40348140977376	26.114382775805815	30.350740382202147
36	18.443673586461582	29.121849342081674	23.844161198953067	28.590315872503673
37	17.719649707791042	29.543580366426447	29.576969273242266	23.159800652540248
38	18.094322541321574	28.850256355096626	22.840701301495105	30.21471980208669
39	21.042182065899397	29.317701050518085	24.911709870567567	24.728407013014948
40	19.192795166899717	23.720913556344343	28.42650855114553	28.659782725610413
41	17.654888673765733	27.48377612850023	24.95114911620236	29.910186081531677
42	18.940249542863288	23.882928184719084	29.983014233982285	27.193808038435336
43	21.649008640780178	23.260415546233553	26.499139507367968	28.591436305618302
44	21.07198558674841	23.77110895987953	28.159397296618984	26.997508156753074
45	20.143594707970315	22.735380588720375	27.23638449679108	29.88464020651823
46	24.507233516188016	25.733211430210464	25.967381951167045	23.79217310243448
47	17.74250654332939	24.160571510523106	33.928059230576174	24.168862715571333
48	20.109981714531568	24.88347495607902	27.28613172708042	27.720411602308985
49	19.506740525617584	21.941665770320178	33.46263131476103	25.088962389301205
50	19.96499766949912	21.275456240364274	30.683284930622783	28.07626115951382
51	19.50741278548636	21.588505252590444	27.11067190133018	31.793410060593025
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	256.0
1	531.0
2	806.0
3	4252.5
4	7699.0
5	4931.5
6	2164.0
7	2077.5
8	1991.0
9	1982.5
10	1974.0
11	1935.0
12	1896.0
13	1969.5
14	2043.0
15	1871.5
16	1700.0
17	1711.5
18	1723.0
19	1643.5
20	1564.0
21	1623.5
22	1683.0
23	1748.5
24	1814.0
25	2065.0
26	2772.0
27	3228.0
28	3749.5
29	4271.0
30	4897.0
31	5523.0
32	5929.0
33	6335.0
34	7059.0
35	7783.0
36	8706.0
37	9629.0
38	10243.0
39	10857.0
40	12680.5
41	14504.0
42	16837.5
43	19171.0
44	22355.5
45	25540.0
46	48339.5
47	71139.0
48	60076.0
49	49013.0
50	46464.0
51	43915.0
52	36946.0
53	29977.0
54	26241.5
55	22506.0
56	20883.5
57	19261.0
58	17900.0
59	16539.0
60	15349.5
61	14160.0
62	13018.0
63	11876.0
64	10879.5
65	9883.0
66	8304.0
67	6725.0
68	5775.5
69	4826.0
70	4248.5
71	3671.0
72	3164.0
73	2657.0
74	2214.5
75	1294.5
76	817.0
77	702.5
78	588.0
79	400.5
80	213.0
81	180.5
82	148.0
83	100.5
84	53.0
85	41.0
86	29.0
87	21.0
88	13.0
89	8.0
90	3.0
91	1.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	446256.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.610891923351545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.40120398760955	26.49493234169996
2	8.986791001400796	6.400552861772639
3	3.507754962530886	3.747428485928623
4	2.033287760567395	2.8962876276255605
5	1.5498140329433754	2.7595130014220066
6	1.2379496709066935	2.6450695162324127
7	1.0118834377840293	2.522385021936955
8	0.8511936258008126	2.4249411371390783
9	0.7636276780140622	2.4474116440294797
>10	5.528293848039108	31.44156228608676
>50	0.07009950432890802	1.7260493307858962
>100	0.048627584084017274	3.354495350879796
>500	0.0012630541320523966	0.320471699852756
>1k	0.005683743594235785	3.511920045544307
>5k	0.001894581198078595	4.975070136240469
>10k+	6.315270660261983E-4	2.331909512823317
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTC	10369	2.323554193108888	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCG	9649	2.1622118246029185	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGC	6988	1.5659173209996058	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5485	1.229115126743394	No Hit
GCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	3523	0.7894571725646267	Illumina PCR Primer Index 11 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCT	2299	0.5151751461044782	Illumina PCR Primer Index 11 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCT	2298	0.5149510594815532	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTC	1572	0.3522641712380338	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTAACACCT	1392	0.31192857911154137	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTAACACCTCGTA	1265	0.2834695780000717	No Hit
CTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGCT	1204	0.2698002940016493	Illumina PCR Primer Index 11 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	1062	0.23797999354630528	Illumina PCR Primer Index 11 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCC	1001	0.22431070954788282	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTT	758	0.16985766017711806	No Hit
TCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	667	0.1494657774909469	Illumina PCR Primer Index 11 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2787637589186476	0.0
2	0.0	0.0	0.0	1.5948244953569253	0.0
3	0.0	0.0	0.0	2.090728191889857	0.0
4	0.0	0.0	0.0	3.2674070488688107	0.0
5	0.0	0.0	0.0	7.34107776702162	0.0
6	0.0	0.0	0.0	8.621284643791904	0.0
7	0.0	0.0	0.0	10.220590871607328	0.0
8	0.0	0.0	0.0	12.054963966871034	0.0
9	0.0	0.0	0.0	12.730809221612706	0.0
10	0.0	0.0	0.0	16.113620881287872	0.0
11	0.0	0.0	0.0	17.840880570793445	0.0
12	0.0	0.0	0.0	20.807115198451115	0.0
13	0.0	0.0	0.0	21.464809436735866	0.0
14	0.0	0.0	0.0	21.774048976372306	0.0
15	0.0	0.0	0.0	22.462891255243626	0.0
16	0.0	0.0	0.0	23.238230970563983	0.0
17	0.0	0.0	0.0	24.124269477609264	0.0
18	0.0	0.0	0.0	25.125936682083825	0.0
19	0.0	0.0	0.0	26.031694811946505	0.0
20	0.0	0.0	0.0	26.654879710300815	0.0
21	0.0	0.0	0.0	27.282322254490698	0.0
22	0.0	0.0	0.0	27.972509053099568	0.0
23	0.0	0.0	0.0	28.56925172994873	0.0
24	0.0	0.0	0.0	29.093390340970206	0.0
25	0.0	0.0	0.0	29.555905130687318	0.0
26	0.0	0.0	0.0	29.961053744935644	0.0
27	0.0	0.0	0.0	30.452027535764223	0.0
28	0.0	0.0	0.0	30.89190957656592	0.0
29	0.0	0.0	0.0	31.335825176580258	0.0
30	0.0	0.0	0.0	31.818507762360618	0.0
31	0.0	0.0	0.0	32.225000896346494	0.0
32	0.0	0.0	0.0	32.638888888888886	0.0
33	0.0	0.0	0.0	33.02633465992614	0.0
34	0.0	0.0	0.0	33.43170736079739	0.0
35	0.0	0.0	0.0	33.878087913663904	0.0
36	0.0	0.0	0.0	34.274497149618156	0.0
37	0.0	0.0	0.0	34.683903409702054	0.0
38	0.0	0.0	0.0	35.078071779427056	0.0
39	0.0	0.0	0.0	35.54394786848804	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCATCG	20	7.028642E-4	45.000004	21
TAGTGCG	20	7.028642E-4	45.000004	1
TTACCAC	20	7.028642E-4	45.000004	39
CGATACG	20	7.028642E-4	45.000004	10
CCCATCG	20	7.028642E-4	45.000004	40
GTGCCTT	20	7.028642E-4	45.000004	34
TCACGAC	25	3.8869577E-5	45.0	25
ACAACGA	35	1.2097007E-7	45.0	13
AACCGAT	35	1.2097007E-7	45.0	16
TAGGTCG	25	3.8869577E-5	45.0	1
CGTTCAT	50	2.1827873E-11	45.0	17
CCATTAC	45	3.8380676E-10	45.0	6
CGAACGG	30	2.1625056E-6	44.999996	2
CGAACCC	30	2.1625056E-6	44.999996	34
GGCCGAT	60	0.0	44.999996	8
CGATGAA	840	0.0	43.660713	19
CCGATGA	825	0.0	42.0	18
CGTTTTT	2260	0.0	41.81416	1
GATGAAT	915	0.0	41.80328	20
TACGGCT	770	0.0	41.493507	7
>>END_MODULE