##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552022_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	370765
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.36878076409585	33.0	31.0	34.0	31.0	34.0
2	32.465858427845134	34.0	31.0	34.0	31.0	34.0
3	32.43811309050207	34.0	31.0	34.0	31.0	34.0
4	35.98892020552102	37.0	35.0	37.0	35.0	37.0
5	36.05593030625868	37.0	35.0	37.0	35.0	37.0
6	36.0494949631168	37.0	35.0	37.0	35.0	37.0
7	36.241597777568	37.0	37.0	37.0	35.0	37.0
8	36.1096921230429	37.0	37.0	37.0	35.0	37.0
9	38.00151578493116	39.0	39.0	39.0	35.0	39.0
10	37.67309212034577	39.0	37.0	39.0	35.0	39.0
11	37.63349561042709	39.0	37.0	39.0	35.0	39.0
12	37.60999554974175	39.0	37.0	39.0	35.0	39.0
13	37.63524334821248	39.0	37.0	39.0	35.0	39.0
14	38.91677207934946	40.0	38.0	41.0	35.0	41.0
15	38.98131430960312	40.0	38.0	41.0	35.0	41.0
16	38.96335414615727	40.0	38.0	41.0	35.0	41.0
17	38.91649697247583	40.0	38.0	41.0	35.0	41.0
18	38.783102504281686	40.0	38.0	41.0	35.0	41.0
19	38.66732566450447	40.0	38.0	41.0	35.0	41.0
20	38.54081695952962	40.0	37.0	41.0	35.0	41.0
21	38.43743341469664	40.0	37.0	41.0	35.0	41.0
22	38.44982401251467	40.0	37.0	41.0	35.0	41.0
23	38.41492859358354	40.0	37.0	41.0	35.0	41.0
24	38.36442220813723	40.0	37.0	41.0	35.0	41.0
25	38.19446279988672	40.0	37.0	41.0	34.0	41.0
26	38.243178293528246	40.0	37.0	41.0	34.0	41.0
27	38.26458268714684	40.0	37.0	41.0	34.0	41.0
28	38.17860639488625	40.0	37.0	41.0	34.0	41.0
29	38.133828705514276	40.0	37.0	41.0	34.0	41.0
30	37.967116637223036	40.0	36.0	41.0	34.0	41.0
31	37.88072229039958	40.0	36.0	41.0	34.0	41.0
32	37.69637371380794	40.0	36.0	41.0	33.0	41.0
33	37.51760818847518	40.0	36.0	41.0	33.0	41.0
34	37.34482219195447	40.0	36.0	41.0	33.0	41.0
35	37.196763448545575	40.0	36.0	41.0	33.0	41.0
36	37.09861772281634	40.0	36.0	41.0	32.0	41.0
37	37.045651558264666	40.0	35.0	41.0	32.0	41.0
38	36.94463878737206	40.0	35.0	41.0	32.0	41.0
39	36.894275349614986	40.0	35.0	41.0	31.0	41.0
40	36.81790082666918	40.0	35.0	41.0	31.0	41.0
41	36.7284506358475	40.0	35.0	41.0	31.0	41.0
42	36.60476582201664	40.0	35.0	41.0	31.0	41.0
43	36.46903024827047	39.0	35.0	41.0	30.0	41.0
44	36.48880827478322	39.0	35.0	41.0	31.0	41.0
45	36.47508259949024	39.0	35.0	41.0	31.0	41.0
46	36.4157727940879	39.0	35.0	41.0	31.0	41.0
47	36.38780629239545	39.0	35.0	41.0	31.0	41.0
48	36.33989723949132	39.0	35.0	41.0	30.0	41.0
49	36.28556902620258	39.0	35.0	41.0	30.0	41.0
50	36.199341901204264	39.0	35.0	41.0	30.0	41.0
51	35.41489622806899	38.0	35.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	6.0
10	3.0
11	7.0
12	3.0
13	8.0
14	10.0
15	19.0
16	24.0
17	42.0
18	95.0
19	146.0
20	258.0
21	418.0
22	604.0
23	891.0
24	1503.0
25	2587.0
26	3775.0
27	4602.0
28	4640.0
29	4437.0
30	4773.0
31	5344.0
32	6524.0
33	8802.0
34	16926.0
35	24278.0
36	24909.0
37	36201.0
38	70392.0
39	148353.0
40	180.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.2657613313015	19.367793615902258	23.960729842352972	15.405715210443272
2	36.10939543915958	23.206613353471877	23.886558871522393	16.797432335846153
3	31.283157795369032	22.743516782867854	29.69401103124621	16.279314390516905
4	27.764486939166318	24.864806548622443	28.883524604533868	18.487181907677368
5	24.157889768451714	28.71441479104015	27.398486912195057	19.729208528313084
6	26.181273852709936	32.527611829595564	26.779226733915014	14.511887583779485
7	78.12360929429693	5.529917872506844	11.923994983345246	4.422477849850984
8	79.17953420630319	5.024476420374092	11.57148058743409	4.224508785888636
9	72.36362655590469	7.6342157431257	13.680093859992178	6.322063840977439
10	34.18310789853411	31.882998664922525	19.608377274014536	14.325516162528826
11	27.02817148328456	24.141167585937183	30.30949523282942	18.521165697948835
12	25.06897900287244	22.139090798753926	33.037638396288756	19.75429180208488
13	24.82219195447251	22.056558736666084	34.07117716073524	19.05007214812617
14	21.615848313621836	25.267757204698395	32.48850349952126	20.627890982158508
15	20.63544293555217	24.34021550038434	35.039984896093216	19.984356667970278
16	23.296967081574582	23.334457135921678	33.36372095532211	20.004854827181635
17	22.89509527598344	22.997855784661443	31.518347201057274	22.588701738297846
18	23.252464499076233	22.94607096139064	32.20800237347107	21.59346216606206
19	23.415101209661106	24.268741655765783	30.988361900395127	21.327795234177984
20	24.201583213086455	24.924413037908106	30.77744663061508	20.096557118390354
21	24.35451026930805	24.523620082801774	31.372162960365728	19.749706687524444
22	22.784243388669374	23.223335535986404	30.903941849958873	23.088479225385353
23	21.24823001092336	25.58466953461087	31.28693377206586	21.880166682399903
24	21.562984639866222	24.5400725526951	32.16700605504835	21.729936752390326
25	21.68678273299799	25.721953258802742	30.693026580178817	21.898237428020444
26	21.00764635281108	25.8311868703896	31.131039877011048	22.030126899788275
27	21.77605761061589	24.412228770245303	31.43069059916659	22.38102301997222
28	20.00242741359082	25.442800695858566	32.49039148786967	22.064380402680943
29	21.050260946961014	24.505819049802437	32.19451674241096	22.24940326082559
30	21.786846115463973	23.76572761722385	31.706336898035143	22.741089369277034
31	21.71105686890618	25.513735115234716	30.261216673634245	22.51399134222486
32	22.00531333863768	26.346877402128033	29.86959394764878	21.778215311585505
33	21.838361226113577	25.67879923941041	28.917508394805335	23.565331139670683
34	21.647674402923688	25.867867786873088	30.53578412201799	21.948673688185238
35	21.948134262942833	25.33491564737772	29.61120925653716	23.10574083314229
36	22.562809326662443	26.819144201852925	28.57119738918183	22.046849082302806
37	22.360524860760858	26.57181772821059	29.290790662549053	21.776866748479495
38	21.750165198980486	26.9305355144094	28.53289819697113	22.78640108963899
39	22.7108815557024	25.945275309158095	28.145051447682494	23.198791687457014
40	23.17209013795801	25.214893530942778	29.57156150122045	22.041454829878766
41	21.91468989791377	25.652906827775006	29.658408965247528	22.773994309063692
42	23.14215203700457	26.09469610130406	28.855744204550053	21.907407657141313
43	22.84708642940946	25.070327565978452	29.47041926826966	22.612166736342427
44	21.992636845441183	25.54583091715777	29.243051528596283	23.21848070880477
45	22.132887408466278	24.791984140897874	29.160519466508433	23.914608984127412
46	22.60056909363074	25.809609860693435	28.84711340067159	22.74270764500425
47	21.20588512939463	25.601931142367807	30.72377381899586	22.4684099092417
48	21.71105686890618	24.893396086469867	30.360470918236622	23.035076126387334
49	21.889336911520772	24.32996642077866	30.615349345272612	23.165347322427955
50	20.679675805429316	24.565964964330504	30.693566005421225	24.060793224818955
51	20.93859992178334	24.232060739282293	29.83480101951371	24.994538319420656
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	223.0
1	453.0
2	683.0
3	3809.0
4	6935.0
5	4493.0
6	2051.0
7	2046.0
8	2041.0
9	1995.0
10	1949.0
11	1938.5
12	1928.0
13	1900.5
14	1873.0
15	1760.5
16	1648.0
17	1604.0
18	1560.0
19	1515.5
20	1471.0
21	1477.0
22	1483.0
23	1636.5
24	1790.0
25	2050.0
26	2814.5
27	3319.0
28	3880.5
29	4442.0
30	5032.5
31	5623.0
32	6483.0
33	7343.0
34	8263.0
35	9183.0
36	10296.0
37	11409.0
38	12421.5
39	13434.0
40	14542.0
41	15650.0
42	17680.0
43	19710.0
44	21285.5
45	22861.0
46	25820.0
47	28779.0
48	31007.0
49	33235.0
50	32676.0
51	32117.0
52	28980.0
53	25843.0
54	23746.5
55	21650.0
56	19920.5
57	18191.0
58	17191.0
59	16191.0
60	15374.5
61	14558.0
62	13160.0
63	11762.0
64	10476.5
65	9191.0
66	7596.0
67	6001.0
68	5212.0
69	4423.0
70	3626.0
71	2829.0
72	2493.5
73	2158.0
74	1638.5
75	924.0
76	729.0
77	630.0
78	531.0
79	408.0
80	285.0
81	219.5
82	154.0
83	117.5
84	81.0
85	45.5
86	10.0
87	8.0
88	6.0
89	4.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	370765.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.764352053266954
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.93410003488952	26.410159742459566
2	8.153895785491763	6.158531820979211
3	3.9453687317557598	4.4698288129792765
4	2.9138160216650033	4.401534962424276
5	2.3092772503166126	4.36041795347784
6	1.977991763416044	4.481854638726348
7	1.7256009618333492	4.561634155829146
8	1.5556266118923938	4.699778482794819
9	1.362852221446676	4.632050797856029
>10	6.05473912573561	31.60457916751097
>50	0.04161748935832563	1.044610299732236
>100	0.02296137343907621	1.6280204100625872
>500	0.001435085839942263	0.38261731341683974
>1k	7.175429199711316E-4	1.1643814417508218
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4297	1.158955133305463	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCC	762	0.20552101735600717	No Hit
GCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	650	0.17531320378137094	Illumina Single End Adapter 2 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGCT	483	0.1302711960406187	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	373	0.10060280770838673	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13377746011624614	0.0
2	0.0	0.0	0.0	0.5305247259045487	0.0
3	0.0	0.0	0.0	0.7929551063342009	0.0
4	0.0	0.0	0.0	1.1745984653351853	0.0
5	0.0	0.0	0.0	2.133157121087481	0.0
6	0.0	0.0	0.0	2.8373767750461885	0.0
7	0.0	0.0	0.0	3.433981093145254	0.0
8	0.0	0.0	0.0	4.332933259611884	0.0
9	0.0	0.0	0.0	4.8138308632152444	0.0
10	0.0	0.0	0.0	5.742451418014106	0.0
11	0.0	0.0	0.0	6.859601095033242	0.0
12	0.0	0.0	0.0	7.7499224576214045	0.0
13	0.0	0.0	0.0	8.096233463244912	0.0
14	0.0	0.0	0.0	8.249969657330114	0.0
15	0.0	0.0	0.0	8.515636589214193	0.0
16	0.0	0.0	0.0	9.008671260771647	0.0
17	0.0	0.0	0.0	9.610939543915958	0.0
18	0.0	0.0	0.0	10.270117190133913	0.0
19	0.0	0.0	0.0	10.651490836513695	0.0
20	0.0	0.0	0.0	11.027739943090637	0.0
21	0.0	0.0	0.0	11.47195663021051	0.0
22	0.0	0.0	0.0	11.963642738661955	0.0
23	0.0	0.0	0.0	12.43213356169002	0.0
24	0.0	0.0	0.0	12.81836203525144	0.0
25	0.0	0.0	0.0	13.1584696505873	0.0
26	0.0	0.0	0.0	13.476460830984585	0.0
27	0.0	0.0	0.0	13.792564023033458	0.0
28	0.0	0.0	0.0	14.117028306339595	0.0
29	0.0	0.0	0.0	14.469812414871955	0.0
30	0.0	0.0	0.0	14.85550146319097	0.0
31	0.0	0.0	0.0	15.202351894056882	0.0
32	0.0	0.0	0.0	15.550550888028805	0.0
33	0.0	0.0	0.0	15.91439321403045	0.0
34	0.0	0.0	0.0	16.242363761412214	0.0
35	0.0	0.0	0.0	16.598114708777796	0.0
36	0.0	0.0	0.0	16.941728588189285	0.0
37	0.0	0.0	0.0	17.268080859843835	0.0
38	0.0	0.0	0.0	17.586072040241124	0.0
39	0.0	0.0	0.0	17.91943684004693	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTC	20	7.0270966E-4	45.000004	45
ACGTTAG	20	7.0270966E-4	45.000004	1
TACGATG	20	7.0270966E-4	45.000004	1
TTAAGCG	20	7.0270966E-4	45.000004	1
TATAGCG	20	7.0270966E-4	45.000004	1
CGTAAAT	20	7.0270966E-4	45.000004	14
CAATGCG	20	7.0270966E-4	45.000004	1
GTTAGCG	25	3.885677E-5	45.0	1
GCTTACG	25	3.885677E-5	45.0	1
CGAATAT	45	3.8380676E-10	45.0	14
CGTTTTT	2025	0.0	41.666668	1
CACGCCG	50	1.0768417E-9	40.5	26
CGTTAGG	50	1.0768417E-9	40.5	2
TACGAAT	50	1.0768417E-9	40.5	12
GGACGAA	35	6.2368454E-6	38.571426	8
TTACGGG	135	0.0	38.333332	3
AACGGGC	65	9.094947E-12	38.076927	4
AGACACG	95	0.0	37.894737	24
TACGGGA	155	0.0	37.741936	4
CGTATGG	30	1.13843074E-4	37.500004	2
>>END_MODULE