FastQCFastQC Report
Sat 18 Jun 2016
SRR3552021_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552021_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328109
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52961.6140977541000097No Hit
GCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGC12190.37152287806795914No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCG8840.2694226613716783No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCC8260.25174560892874015No Hit
CTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGCT8090.24656440390236173No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGC7220.2200488252379545No Hit
CCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGC7050.21486762021157602No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTC6680.2035908798600465No Hit
TCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGC5170.15756958815515576No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT4320.1316635630232636No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3910.11916771560670388No Hit
ACTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGC3550.1081957520214319No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3370.10270977022879592No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCAAT253.8846927E-545.017
GACCCAC406.7902874E-945.012
TGTTGCG253.8846927E-545.01
ACGATTG253.8846927E-545.01
TCTACTC207.0259115E-445.028
CGGGATA207.0259115E-445.06
TCGTAAG207.0259115E-445.01
CGAGCCA207.0259115E-445.024
CCGACAG207.0259115E-445.01
CGTAAGG406.7902874E-945.02
TATAGAG351.2083729E-745.01
CACAACG253.8846927E-545.012
ACATAGT406.7902874E-945.035
GGTAGTC207.0259115E-445.08
ATAGCGG302.160743E-644.9999962
CTACGAA600.044.99999611
TACGAAT600.044.99999612
CGAATAT600.044.99999614
CGTTTTT24650.043.630831
ACCTATG650.041.53846434