##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3552021_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	328109
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25237344906723	33.0	31.0	34.0	30.0	34.0
2	32.351200363293906	34.0	31.0	34.0	30.0	34.0
3	32.32411789984426	34.0	31.0	34.0	30.0	34.0
4	35.9203862131182	37.0	35.0	37.0	35.0	37.0
5	35.99458106909594	37.0	35.0	37.0	35.0	37.0
6	35.99944835405307	37.0	35.0	37.0	35.0	37.0
7	36.17389647952357	37.0	36.0	37.0	35.0	37.0
8	35.99452316151035	37.0	35.0	37.0	35.0	37.0
9	37.904687161888276	39.0	38.0	39.0	35.0	39.0
10	37.632993913607976	39.0	37.0	39.0	35.0	39.0
11	37.598316413143195	39.0	37.0	39.0	35.0	39.0
12	37.509608087556266	39.0	37.0	39.0	35.0	39.0
13	37.48048666754036	39.0	37.0	39.0	35.0	39.0
14	38.70460121484019	40.0	38.0	41.0	35.0	41.0
15	38.75622125574123	40.0	38.0	41.0	35.0	41.0
16	38.769527809356035	40.0	38.0	41.0	35.0	41.0
17	38.73342700139283	40.0	38.0	41.0	35.0	41.0
18	38.59151684348799	40.0	38.0	41.0	35.0	41.0
19	38.430131450219285	40.0	37.0	41.0	35.0	41.0
20	38.263452084520694	40.0	36.0	41.0	34.0	41.0
21	38.126826755742755	40.0	36.0	41.0	34.0	41.0
22	38.15288516925778	40.0	36.0	41.0	34.0	41.0
23	38.138679524182514	40.0	36.0	41.0	34.0	41.0
24	38.0583098909204	40.0	35.0	41.0	34.0	41.0
25	37.88690038980948	40.0	35.0	41.0	34.0	41.0
26	37.91461374116528	40.0	35.0	41.0	34.0	41.0
27	37.89226750866328	40.0	35.0	41.0	34.0	41.0
28	37.79581175767809	40.0	35.0	41.0	34.0	41.0
29	37.722128926667665	40.0	35.0	41.0	34.0	41.0
30	37.52959230011978	40.0	35.0	41.0	33.0	41.0
31	37.40324709166771	40.0	35.0	41.0	33.0	41.0
32	37.17177828099808	40.0	35.0	41.0	33.0	41.0
33	36.94439347899631	40.0	35.0	41.0	32.0	41.0
34	36.73675821144803	40.0	35.0	41.0	31.0	41.0
35	36.59376609602297	40.0	35.0	41.0	31.0	41.0
36	36.459204105952594	39.0	35.0	41.0	30.0	41.0
37	36.40391150501815	39.0	35.0	41.0	30.0	41.0
38	36.297568795735565	39.0	35.0	41.0	30.0	41.0
39	36.266972256170966	39.0	35.0	41.0	30.0	41.0
40	36.16205894992213	39.0	35.0	41.0	30.0	41.0
41	36.08243297196968	39.0	35.0	41.0	29.0	41.0
42	35.96929069303189	39.0	35.0	41.0	29.0	41.0
43	35.85822394387231	39.0	35.0	41.0	29.0	41.0
44	35.880155679972205	39.0	35.0	41.0	29.0	41.0
45	35.85739495106809	39.0	35.0	41.0	29.0	41.0
46	35.768512902724396	39.0	35.0	41.0	29.0	41.0
47	35.724484851070834	39.0	35.0	41.0	29.0	41.0
48	35.712507124156915	38.0	35.0	41.0	29.0	41.0
49	35.68342837288828	38.0	35.0	41.0	28.0	41.0
50	35.595622186529475	38.0	35.0	41.0	28.0	41.0
51	34.81099268840538	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	7.0
11	6.0
12	7.0
13	10.0
14	16.0
15	12.0
16	31.0
17	34.0
18	78.0
19	153.0
20	266.0
21	423.0
22	717.0
23	1068.0
24	1768.0
25	2769.0
26	4150.0
27	5104.0
28	4814.0
29	4701.0
30	4783.0
31	5283.0
32	6685.0
33	8972.0
34	17423.0
35	26238.0
36	23776.0
37	32479.0
38	60155.0
39	116048.0
40	129.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.95072064466382	19.50297004958718	22.88721126211107	16.659098043637936
2	36.07063506334785	23.996598691288565	23.238923650372286	16.6938425949913
3	31.41334129816616	22.722631808331986	30.292067575104614	15.57195931839724
4	28.165944853691915	23.025884690758254	30.429826673453032	18.378343782096803
5	24.944149657583303	26.611583345778385	28.850168693940127	19.59409830269819
6	25.0346683571618	32.347482086745565	27.96448741119567	14.65336214489697
7	72.56856715298879	6.438409187190842	15.88344117351246	5.109582486307904
8	72.23056971920917	6.712098723290126	15.251943713826806	5.805387843673901
9	65.41118957419638	8.639506993102902	17.75172275067127	8.197580682029447
10	36.49549387551088	24.298632466649803	23.729919020813206	15.47595463702611
11	29.316172369547928	23.167605887068017	28.53167697320098	18.984544770183078
12	26.38635331551405	20.804976395039454	33.674480127030954	19.13419016241554
13	23.844515084926048	23.077696741022038	34.556808865346575	18.52097930870534
14	19.779097799816526	26.87765346272123	32.69888969824052	20.644359039221722
15	18.573705689267896	23.873164100954256	37.81670115723738	19.736429052540466
16	19.631585844947868	23.764054018634052	35.31052180830151	21.29383832811657
17	20.132334071909032	23.06428656330671	32.796723040209194	24.006656324575065
18	20.99393799011914	23.617456394064167	33.79517172646895	21.593433889347747
19	23.007293308016543	24.31295697466391	31.802541228677057	20.87720848864249
20	24.847230645913402	24.00238944984746	32.060382372930945	19.089997531308192
21	22.112163945518105	25.56772292134626	32.66627858425096	19.653834548884667
22	20.35695454864085	24.548549414980997	31.060105026073654	24.0343910103045
23	21.480666485832455	25.049297641942157	31.784864176234116	21.685171695991272
24	21.921678466607133	23.564425236735353	32.22861914790512	22.285277148752396
25	20.585232346567757	25.928883389361463	30.851332941187227	22.634551322883553
26	20.03023385521275	26.091329405776737	31.91256564129603	21.96587109771448
27	22.74853783346388	25.43148770682913	30.992139807198214	20.82783465250877
28	19.525828307056496	25.666165816847453	33.25845984108939	21.54954603500666
29	21.70406785549924	24.847230645913402	31.25973380797235	22.18896769061501
30	22.605597530089085	25.087699514490613	29.95346058779247	22.353242367627832
31	22.614131279544296	25.98313365375531	28.501504073341483	22.901230993358915
32	23.71833750369542	27.401259947151708	27.652700779314195	21.22770176983868
33	22.373967187733346	25.407410342294785	28.316199799456886	23.90242267051498
34	21.450493585972954	25.281842314596677	29.092770999881136	24.174893099549237
35	22.776577295959573	25.66494670978242	27.577116141282314	23.981359852975686
36	22.210911617785552	27.35493387868056	28.63377718989726	21.800377313636627
37	23.0277133513558	26.31930242693739	29.254912239530157	21.398071982176656
38	21.638540850753866	27.221441655059753	27.448805122687887	23.691212371498498
39	23.282506727947116	23.630257018246986	28.78829901038984	24.29893724341606
40	21.65652267996306	24.64424931958587	29.62917810849443	24.070049891956636
41	19.454815320518488	26.900511720190543	28.996461541743752	24.648211417547216
42	20.894580764319173	25.88956718651424	30.60415898375235	22.611693065414237
43	23.353214937718867	23.58240706594455	30.33260288501687	22.731775111319713
44	22.22645523286469	23.844210308159788	29.363412768317847	24.565921690657678
45	22.588225254412407	23.44403841406362	27.982164463638608	25.985571867885366
46	22.544946953603834	25.66494670978242	28.29974185407898	23.49036448253477
47	20.463016863298478	24.792066051220786	31.641619096093066	23.103297989387674
48	20.457226104739583	24.12064283515539	30.542594076968328	24.879536983136703
49	21.043616603019117	22.985349380846	32.21277075605972	23.758263260075157
50	20.287160669167868	22.888735145942356	31.575787314581433	25.24831687030834
51	20.08265545900905	22.893306797436217	28.880646370565877	28.14339137298885
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	246.0
1	535.0
2	824.0
3	4521.5
4	8219.0
5	5276.0
6	2333.0
7	2232.0
8	2131.0
9	2119.5
10	2108.0
11	2070.0
12	2032.0
13	2035.5
14	2039.0
15	1941.5
16	1844.0
17	1758.0
18	1672.0
19	1726.5
20	1781.0
21	1723.5
22	1666.0
23	1685.0
24	1704.0
25	1944.0
26	2626.5
27	3069.0
28	3691.5
29	4314.0
30	4603.5
31	4893.0
32	5087.5
33	5282.0
34	5859.5
35	6437.0
36	7175.5
37	7914.0
38	8261.0
39	8608.0
40	10144.5
41	11681.0
42	13573.0
43	15465.0
44	17585.0
45	19705.0
46	23627.5
47	27550.0
48	29646.0
49	31742.0
50	31096.5
51	30451.0
52	27048.0
53	23645.0
54	20703.0
55	17761.0
56	16249.5
57	14738.0
58	14146.0
59	13554.0
60	13044.0
61	12534.0
62	11326.0
63	10118.0
64	8965.0
65	7812.0
66	6808.0
67	5804.0
68	5039.0
69	4274.0
70	3891.0
71	3508.0
72	3002.0
73	2496.0
74	2146.5
75	1383.0
76	969.0
77	833.5
78	698.0
79	487.5
80	277.0
81	225.0
82	173.0
83	99.0
84	25.0
85	15.5
86	6.0
87	12.5
88	19.0
89	11.0
90	3.0
91	3.0
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	328109.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.48878746651814
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.74177926978497	22.658258442328645
2	11.090607759426039	7.206407967410191
3	3.942229034345749	3.8423472372358827
4	2.1011936032447758	2.730609296073078
5	1.4862074591585401	2.414253913587787
6	1.089726771423182	2.124234088400565
7	0.9583107377728041	2.179404772046798
8	0.8577932090620778	2.2294929007552327
9	0.8120159167436444	2.3743271284662733
>10	7.785795445113493	44.18657866618212
>50	0.08159959334687471	1.7846239741118388
>100	0.043785147649542534	2.504292989759113
>500	0.006965818944245403	1.6588597121680877
>1k	9.951169920350575E-4	0.39410446094969775
>5k	9.951169920350575E-4	1.7122044505246916
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5296	1.6140977541000097	No Hit
GCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGC	1219	0.37152287806795914	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCG	884	0.2694226613716783	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCC	826	0.25174560892874015	No Hit
CTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGCT	809	0.24656440390236173	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGC	722	0.2200488252379545	No Hit
CCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGC	705	0.21486762021157602	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTC	668	0.2035908798600465	No Hit
TCTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGC	517	0.15756958815515576	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	432	0.1316635630232636	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	391	0.11916771560670388	No Hit
ACTGTCTCTTATACACATCTGACGCAGTGCCATTCGTATGCCGTCTTCTGC	355	0.1081957520214319	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	337	0.10270977022879592	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.25692681395511857	0.0
2	0.0	0.0	0.0	1.1392555522707393	0.0
3	0.0	0.0	0.0	1.569905122992664	0.0
4	0.0	0.0	0.0	2.1773252181439706	0.0
5	0.0	0.0	0.0	3.8514639951967182	0.0
6	0.0	0.0	0.0	4.702705503354069	0.0
7	0.0	0.0	0.0	5.63227464043961	0.0
8	0.0	0.0	0.0	6.965977769582669	0.0
9	0.0	0.0	0.0	7.567302329408824	0.0
10	0.0	0.0	0.0	9.072899554721145	0.0
11	0.0	0.0	0.0	10.881444885693474	0.0
12	0.0	0.0	0.0	12.470855721726622	0.0
13	0.0	0.0	0.0	12.994766982923357	0.0
14	0.0	0.0	0.0	13.210244156667448	0.0
15	0.0	0.0	0.0	13.70611595536849	0.0
16	0.0	0.0	0.0	14.557967017058356	0.0
17	0.0	0.0	0.0	15.547272400330378	0.0
18	0.0	0.0	0.0	16.693233041458782	0.0
19	0.0	0.0	0.0	17.318025412286772	0.0
20	0.0	0.0	0.0	17.915387874151577	0.0
21	0.0	0.0	0.0	18.61058367798506	0.0
22	0.0	0.0	0.0	19.287797652609346	0.0
23	0.0	0.0	0.0	20.008289928042206	0.0
24	0.0	0.0	0.0	20.548049581084335	0.0
25	0.0	0.0	0.0	21.036606737395196	0.0
26	0.0	0.0	0.0	21.517544474549616	0.0
27	0.0	0.0	0.0	21.94575583114148	0.0
28	3.047767662575547E-4	0.0	0.0	22.42425535416584	0.0
29	3.047767662575547E-4	0.0	0.0	22.86435300464175	0.0
30	3.047767662575547E-4	0.0	0.0	23.373634981058125	0.0
31	3.047767662575547E-4	0.0	0.0	23.86889722622665	0.0
32	3.047767662575547E-4	0.0	0.0	24.356844829004995	0.0
33	3.047767662575547E-4	0.0	0.0	24.78749439972692	0.0
34	3.047767662575547E-4	0.0	0.0	25.246792986477054	0.0
35	3.047767662575547E-4	0.0	0.0	25.711882331786082	0.0
36	3.047767662575547E-4	0.0	0.0	26.18245765888775	0.0
37	3.047767662575547E-4	0.0	0.0	26.651204325391866	0.0
38	3.047767662575547E-4	0.0	0.0	27.083987333477594	0.0
39	3.047767662575547E-4	0.0	0.0	27.504884047679276	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCAAT	25	3.8846927E-5	45.0	17
GACCCAC	40	6.7902874E-9	45.0	12
TGTTGCG	25	3.8846927E-5	45.0	1
ACGATTG	25	3.8846927E-5	45.0	1
TCTACTC	20	7.0259115E-4	45.0	28
CGGGATA	20	7.0259115E-4	45.0	6
TCGTAAG	20	7.0259115E-4	45.0	1
CGAGCCA	20	7.0259115E-4	45.0	24
CCGACAG	20	7.0259115E-4	45.0	1
CGTAAGG	40	6.7902874E-9	45.0	2
TATAGAG	35	1.2083729E-7	45.0	1
CACAACG	25	3.8846927E-5	45.0	12
ACATAGT	40	6.7902874E-9	45.0	35
GGTAGTC	20	7.0259115E-4	45.0	8
ATAGCGG	30	2.160743E-6	44.999996	2
CTACGAA	60	0.0	44.999996	11
TACGAAT	60	0.0	44.999996	12
CGAATAT	60	0.0	44.999996	14
CGTTTTT	2465	0.0	43.63083	1
ACCTATG	65	0.0	41.538464	34
>>END_MODULE