FastQCFastQC Report
Sat 18 Jun 2016
SRR3552020_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552020_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences525707
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57961.1025152794237094No Hit
GCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC13410.25508505688529926TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCC11850.22541073259439193No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCG11060.21038335042143247No Hit
GAATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTC10190.19383420802842646No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGC9650.18356232654311241No Hit
CTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGCT8380.1594043830498738TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC6450.1226919177412513TruSeq Adapter, Index 16 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT5580.1061427753482453No Hit
TCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC5490.10443079510069296TruSeq Adapter, Index 16 (95% over 21bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA5480.10424057507318715No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG302.1632459E-645.0000041
TTATGCG253.887908E-545.01
TAACGCG253.887908E-545.01
CTACGAA900.045.011
CGAATAT900.045.014
TCATACG207.029791E-445.031
TAGTGCG253.887908E-545.01
TCGACTC207.029791E-445.028
CGTCATA207.029791E-445.037
TTCGACT207.029791E-445.027
TTCGAAT207.029791E-445.016
AGTACGC207.029791E-445.01
GCTACGA950.042.6315810
TACGGGA2350.041.170214
CGTTTTT22800.040.559211
GACCTAT850.039.70588333
AGGTACG403.4534423E-739.3751
GGATCGA403.4534423E-739.3758
AGTACGG1150.039.1304322
TACGAAT1100.038.86363612