Basic Statistics
Measure | Value |
---|---|
Filename | SRR3552020_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 525707 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 5796 | 1.1025152794237094 | No Hit |
GCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC | 1341 | 0.25508505688529926 | TruSeq Adapter, Index 16 (95% over 21bp) |
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCC | 1185 | 0.22541073259439193 | No Hit |
GAATGATACCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCG | 1106 | 0.21038335042143247 | No Hit |
GAATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTC | 1019 | 0.19383420802842646 | No Hit |
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGC | 965 | 0.18356232654311241 | No Hit |
CTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGCT | 838 | 0.1594043830498738 | TruSeq Adapter, Index 13 (95% over 22bp) |
CCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC | 645 | 0.1226919177412513 | TruSeq Adapter, Index 16 (95% over 21bp) |
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT | 558 | 0.1061427753482453 | No Hit |
TCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC | 549 | 0.10443079510069296 | TruSeq Adapter, Index 16 (95% over 21bp) |
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA | 548 | 0.10424057507318715 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ACGTTAG | 30 | 2.1632459E-6 | 45.000004 | 1 |
TTATGCG | 25 | 3.887908E-5 | 45.0 | 1 |
TAACGCG | 25 | 3.887908E-5 | 45.0 | 1 |
CTACGAA | 90 | 0.0 | 45.0 | 11 |
CGAATAT | 90 | 0.0 | 45.0 | 14 |
TCATACG | 20 | 7.029791E-4 | 45.0 | 31 |
TAGTGCG | 25 | 3.887908E-5 | 45.0 | 1 |
TCGACTC | 20 | 7.029791E-4 | 45.0 | 28 |
CGTCATA | 20 | 7.029791E-4 | 45.0 | 37 |
TTCGACT | 20 | 7.029791E-4 | 45.0 | 27 |
TTCGAAT | 20 | 7.029791E-4 | 45.0 | 16 |
AGTACGC | 20 | 7.029791E-4 | 45.0 | 1 |
GCTACGA | 95 | 0.0 | 42.63158 | 10 |
TACGGGA | 235 | 0.0 | 41.17021 | 4 |
CGTTTTT | 2280 | 0.0 | 40.55921 | 1 |
GACCTAT | 85 | 0.0 | 39.705883 | 33 |
AGGTACG | 40 | 3.4534423E-7 | 39.375 | 1 |
GGATCGA | 40 | 3.4534423E-7 | 39.375 | 8 |
AGTACGG | 115 | 0.0 | 39.130432 | 2 |
TACGAAT | 110 | 0.0 | 38.863636 | 12 |