FastQCFastQC Report
Sat 18 Jun 2016
SRR3552019_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3552019_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157394
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30721.9517897759762124No Hit
GCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC5100.3240275995273009TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCC4850.3081438936681195No Hit
CTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGCT3790.24079698082519027TruSeq Adapter, Index 27 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC3410.21665374791923453TruSeq Adapter, Index 20 (95% over 21bp)
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG2460.15629566565434513No Hit
TCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC2240.1423180044982655TruSeq Adapter, Index 20 (95% over 21bp)
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG2190.13914126332642923No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA2020.12834034334218586No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG1910.12135151276414602No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC1890.12008081629541151No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGC1720.10927989631116813No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG1610.10229106573312832No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA1580.10038502103002656No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTAAG207.014718E-445.0000041
GGTACTT302.1535325E-645.0000047
AAATCAC207.014718E-445.00000419
CATATTA406.7502697E-945.00000428
ATTAGTC302.1535325E-645.00000431
CGTCTCG207.014718E-445.00000442
TCGATGG207.014718E-445.0000042
TGCGCCA207.014718E-445.00000419
CAAGGGT207.014718E-445.0000044
TAGATGA207.014718E-445.00000416
TATCGCG207.014718E-445.0000041
CGTCACG207.014718E-445.00000437
AAGGACC207.014718E-445.00000437
AGTACGG207.014718E-445.0000042
GTAACGG207.014718E-445.0000042
TTCAGTG253.875424E-545.017
ACGTTGG253.875424E-545.02
CTATCAC253.875424E-545.019
TATGGGT253.875424E-545.04
ATGTACG253.875424E-545.01