Basic Statistics
Measure | Value |
---|---|
Filename | SRR3552019_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 157394 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 47 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 3072 | 1.9517897759762124 | No Hit |
GCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC | 510 | 0.3240275995273009 | TruSeq Adapter, Index 20 (95% over 21bp) |
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCC | 485 | 0.3081438936681195 | No Hit |
CTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGCT | 379 | 0.24079698082519027 | TruSeq Adapter, Index 27 (95% over 23bp) |
CCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC | 341 | 0.21665374791923453 | TruSeq Adapter, Index 20 (95% over 21bp) |
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG | 246 | 0.15629566565434513 | No Hit |
TCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC | 224 | 0.1423180044982655 | TruSeq Adapter, Index 20 (95% over 21bp) |
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG | 219 | 0.13914126332642923 | No Hit |
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAAAAGA | 202 | 0.12834034334218586 | No Hit |
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG | 191 | 0.12135151276414602 | No Hit |
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC | 189 | 0.12008081629541151 | No Hit |
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGC | 172 | 0.10927989631116813 | No Hit |
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG | 161 | 0.10229106573312832 | No Hit |
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA | 158 | 0.10038502103002656 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AAGTAAG | 20 | 7.014718E-4 | 45.000004 | 1 |
GGTACTT | 30 | 2.1535325E-6 | 45.000004 | 7 |
AAATCAC | 20 | 7.014718E-4 | 45.000004 | 19 |
CATATTA | 40 | 6.7502697E-9 | 45.000004 | 28 |
ATTAGTC | 30 | 2.1535325E-6 | 45.000004 | 31 |
CGTCTCG | 20 | 7.014718E-4 | 45.000004 | 42 |
TCGATGG | 20 | 7.014718E-4 | 45.000004 | 2 |
TGCGCCA | 20 | 7.014718E-4 | 45.000004 | 19 |
CAAGGGT | 20 | 7.014718E-4 | 45.000004 | 4 |
TAGATGA | 20 | 7.014718E-4 | 45.000004 | 16 |
TATCGCG | 20 | 7.014718E-4 | 45.000004 | 1 |
CGTCACG | 20 | 7.014718E-4 | 45.000004 | 37 |
AAGGACC | 20 | 7.014718E-4 | 45.000004 | 37 |
AGTACGG | 20 | 7.014718E-4 | 45.000004 | 2 |
GTAACGG | 20 | 7.014718E-4 | 45.000004 | 2 |
TTCAGTG | 25 | 3.875424E-5 | 45.0 | 17 |
ACGTTGG | 25 | 3.875424E-5 | 45.0 | 2 |
CTATCAC | 25 | 3.875424E-5 | 45.0 | 19 |
TATGGGT | 25 | 3.875424E-5 | 45.0 | 4 |
ATGTACG | 25 | 3.875424E-5 | 45.0 | 1 |